l******l 发帖数: 2651 | 1 这个是纯扯淡。
有tg政府资金来源的在湾区的incubator就有好几家。
拿着RMB资本的华人VC少说也有30~50。
体制问题决定了,大部分中国资本现在以跟投和占小股或找代理人为主。 |
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b*********g 发帖数: 506 | 2 POSITION TITLE: Investment Manager
LOCATION: Palo Alto, US
Established in 2008, We now managers 4 USD funds and 4 RMB funds aggregating
15.5 billion RMB. We focus on early stage investment and have already more
than 300 startups. We put our attention to Internet and mobile Internet,
specifically including trading platform, enterprise service SAAS, O2O,
Internet finance, mobile healthcare, new culture, social network, etc. Our
star ortfolio includes MOMO, Cheetah Mobile, Didi Chuxing, Koudai, ele... 阅读全帖 |
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c*********l 发帖数: 3438 | 3 【 以下文字转载自 JobHunting 讨论区 】
发信人: octane (octane), 信区: JobHunting
标 题: 同学们,学习一下阿三如何包装申请intern
发信站: BBS 未名空间站 (Wed Apr 19 02:05:58 2017, 美东)
阿三简历包装和相关技术令人发指,所有网上材料都是包装
举一个今天我发觉的阿三的包装简历为例,它实际经验美国学习9个月美国工作0个月本
国实习3个月,但是简历写得好于是HR发给我看,而且在github上像模像样搞了一个项
目,把别人的很复杂的代码拷贝过来,每隔一两周加一两个无关文件,看起来很厉害,
实际上什么也没做。但是这个很容易糊弄HR和没时间的经理。另外还在阿三国参加假的
高科技公司实际是一个培训公司,然后在美国某些创投网站上发布自己的一个假的公司
,自己作为唯一员工,开发机器人技术,实际上都是骗人的。
简历如下(人名省略):
OBJECTIVE: Summer Internship/ Co-op in Robotics Engineering with focus on
perception, learnin... 阅读全帖 |
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r*********e 发帖数: 29495 | 4 http://realestate.yahoo.com/promo/10-best-cities-for-the-next-decade
1. Austin, Tex.
Austin is arguably the the country's best crucible for small business, offer
ing a dozen community programs that form a neural network of business brainp
ower to help entrepreneurs. Now overlay that net with a dozen venture-capita
l funds and 20 or so business associations, plus incubators, educational opp
ortunities and networking events. Mix all these elements in what many call a
classless society, where hippi |
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e****c 发帖数: 840 | 5 LOL
建议你加入xbox incubation组 |
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h***o 发帖数: 1494 | 6 5月20日上午消息,微软Pioneer Studios部门已经被解散,而该部门位于西雅图的
办公室也已经关闭。
微软的双屏平板电脑Courier就是由Pioneer Studios开发的,该部门的办事处周围
坐落着多家创业企业。一年前,负责创立Pioneer Studios的微软前首席设计师J·阿拉
德(J Allard)刚刚离职。其他一些参与创建这一部门的人要么已经离开微软,要么被调
到了其他部门。
Pioneer Studios联合创始人乔治·派施尼格(Georg Petschnigg)在LinkedIn上透
露,他目前正在从事一个新项目,但并未披露详情。微软发言人称,他目前任职于微软
的创业业务集团。
派施尼格的资料显示,在Pioneer Studios工作期间,他为Courier的开发工作融资
2000万美元。
Courier是一款非常独特的平板电脑,它配备两个屏幕,并通过一个铰链相连,而
且提供了非常先进的用户界面。网上曝光的视频显示,这是一款令人印象深刻的产品。
但就在视频曝光后不久,微软就终止了这一项目。
乔纳森·哈里斯(Jonathan Harris)也在LinkedI... 阅读全帖 |
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Q*K 发帖数: 3464 | 7 This is a Seattle Technical Forum Event Announcement.
Your can stop reading if you are not interested in technical events around
Seattle area.
Sea-Tech Forum and ChinaSoft will co-host a special event about Mobile
Development.
Please feel free to forward this event announcement to your friends, team,
company and organization.
--------------------------------------
Event Details
--------------------------------------
What: Carnival of Mobile Development
When: Wed, August 17th, 2011 6:00 PM -- 9:0... 阅读全帖 |
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t******4 发帖数: 2300 | 8 WASHINGTON (AP) - Federal health officials said Wednesday more illnesses and
possibly more deaths may be linked to an outbreak of listeria in cantaloupe
in coming weeks.
So far, the outbreak has caused at least 72 illnesses - including up to 16
deaths - in 18 states, making it the deadliest food outbreak in the United
States in more than a decade.
The heads of the Centers for Disease Control and Prevention and the Food and
Drug Administration said consumers who have cantaloupes produced by Jense... 阅读全帖 |
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c*****n 发帖数: 96 | 9 An interesting comment from original blog post:
Don't be surprised - you should be angry.
Angry that no one did any basic research. Like checking Wikipedia...
"Appirio was founded by Chris Barbin (former Borland Software CIO), Narinder
Singh, Glenn Weinstein, and Mike O’Brien in 2006. In 2007, Appirio moved
its headquarters into Salesforce.com’s Startup Incubator in San Mateo."
"In early 2008, Appirio secured a Series A investment of $1.1 million from
Salesforce.com"
"In October, 2009, Appirio's... 阅读全帖 |
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s*****n 发帖数: 742 | 10 准确预测未来市场走向是异常重要的,比当前能够盈利,或者盈利多少都重要的多。
所以,保持一些有潜力的incubation projects and encourage innovation are super
critical.
I agree the review system in MS sucks though. It breaks the trust between
company and employees. In 2008-2010, lots of companies laid off ppl, do you
know what Monsanto did?
They announced we will need to adjust our product lines, and reorg groups.
If your group is closed, then we will assign you other tasks and we teach
you to the level that the position requires.
As for MS, while s... 阅读全帖 |
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s******m 发帖数: 2310 | 11 7/11买了PCC的桃子,收据还健在,桃子早已吃完包装销毁。昨儿开始嗓子痛,今儿加剧
,开始出现感冒症状。上周4吃了一堆生蚝和clam,周末吃了两只大螃蟹,搞不明白到底
什么引起的,打算明儿去看医生。今儿手欠给PCC发了email询问怎么个说法,PCC回了个
不痛不痒的email。
有木有人知道如果真的是得了李斯特啥病,是那些某个公司需要负责的还是自己看病自
己消化账单了?
email如下:
"Hi, XXX … and thank you for contacting us. PCC issued the “Sweet 2 Eat”
and “Sweet O” Peaches and Nectarines recall after the company that distrib
uted them – the Wawona Packing Company (Cutler, CA) – voluntarily recalled
them due to the potential of the fruit being contaminated with Listeria mon
ocy... 阅读全帖 |
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c*********l 发帖数: 3438 | 12 【 以下文字转载自 JobHunting 讨论区 】
发信人: octane (octane), 信区: JobHunting
标 题: 同学们,学习一下阿三如何包装申请intern
发信站: BBS 未名空间站 (Wed Apr 19 02:05:58 2017, 美东)
阿三简历包装和相关技术令人发指,所有网上材料都是包装
举一个今天我发觉的阿三的包装简历为例,它实际经验美国学习9个月美国工作0个月本
国实习3个月,但是简历写得好于是HR发给我看,而且在github上像模像样搞了一个项
目,把别人的很复杂的代码拷贝过来,每隔一两周加一两个无关文件,看起来很厉害,
实际上什么也没做。但是这个很容易糊弄HR和没时间的经理。另外还在阿三国参加假的
高科技公司实际是一个培训公司,然后在美国某些创投网站上发布自己的一个假的公司
,自己作为唯一员工,开发机器人技术,实际上都是骗人的。
简历如下(人名省略):
OBJECTIVE: Summer Internship/ Co-op in Robotics Engineering with focus on
perception, learnin... 阅读全帖 |
|
m**o 发帖数: 9805 | 13 incubation period is 27days, if i remember correctly |
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F*********n 发帖数: 2914 | 14 美帝所谓的self monitoring 就是个joke. 万一这个lab worker被感染了,游轮上的几
千人怎么隔离。
http://news.yahoo.com/texas-health-worker-isolated-cruise-ship-
(Reuters) - A Texas health worker who may have had contact with specimens
from the first patient diagnosed with Ebola in the United States has been
isolated on a cruise ship despite showing no symptoms of the disease, the
Department of State said on Friday.
The Texas Health Presbyterian Hospital worker, who did not have direct
contact with now deceased Liberian patient T... 阅读全帖 |
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s****y 发帖数: 18685 | 15 大家还是要小心
Utah's number of confirmed H1N1 swine flu cases continues to grow, up to 60
as of Friday.
Summit County Health Department: 32 confirmed cases.
Salt Lake Valley Health Department: 18 confirmed, 1 probable.
Weber-Morgan Health Department: 1 confirmed, 1 probable.
Utah County Health Department: 3 confirmed (2 were not present in Utah durin
g their incubation period or illness).
Central Utah Health Department: 3 confirmed.
Southwest Utah Public Health Department: 2 confirmed.
Southeast Utah |
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F*V 发帖数: 3978 | 16 大棋局与9/11
来源: ognc 于 2013-02-22 16:08:44[档案] [博客] [旧帖] [转至博客] [给我悄悄
话] 本文已被阅读:63次
字体:调大/调小/重置 | 加入书签| 打印| 所有跟帖 | 加跟贴| 查看当前最热讨论主题
The Moral Decoding of 9-11
By Prof. John McMurtry
(Journal of 9/11 Studies) - I was sceptical of the 9-11 event from the first
time I saw it on television. It was on every major network within minutes.
All the guilty parties were declared before any evidence was shown. The
first questions of any criminal investigation were erased. Who had the most
compelling motives for... 阅读全帖 |
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i******k 发帖数: 4625 | 17 真的啊,我根本都没注意到
也就是昨下午实验incubation中间一小时闲来无聊,发几句感想灌水而已 |
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p****u 发帖数: 22764 | 18 ☆─────────────────────────────────────☆
icebreak (茶坪摩天龙门岷-邛崃大雪沙鲁里) 于 (Tue Oct 19 17:50:28 2010, 美东) 提到:
来米国已经好几年了,开始时在中国超市买鱼,花鲢和草鱼嫌太大,肉柴且腥;
tilapia,国内都没人吃的罗非鱼,卖得还不便宜
去年夏天时见渔版的众高手钓的鲈鱼、墙眼,心想那得多美味啊,才买了permit开始钓
鱼。第一次搞了10来条大个的sunfish,吃着还不错,只是收拾起来麻烦点;第二次
搞了更多,怎么觉得鱼肚子里臭臭的
又向篮球学习钓bass,第一次钓到keeper那个兴奋哦,拿回家清蒸觉得好好吃啊。那一
个月钓了不少bass,自己吃不了还送人。但是天凉下来后吃冰箱里冻的大bass,觉得腥
味挺重的
然后再向crappie进军...这一年以来钓了不少鱼,MA的淡水鱼除了传说中的墙眼,大部
分都尝过了。但奇怪的是,为啥自己越来越不喜欢吃鱼了呢?除了crappie,总觉得鱼
腥,不干净,于是fillet去红肉去脂肪,上重口味,但我仍然尝得出腥味。然而偶尔在
中国店买条ti... 阅读全帖 |
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p****u 发帖数: 22764 | 19 They may not accept other geese's babies, but they may accept their eggs.
The number of eggs laid by one goose in a nest can range from 1 to 10, but
the average for giant Canada geese is between 5 and 6. The goose lay’s one
egg per day. If more than one goose is laying eggs in a nest, as sometimes
occurs when geese are nesting close to each other (such as on an island),
occasionally 15 or more eggs will be found in a nest. These nests, which
are referred to as “dump nests” because 2 or more h... 阅读全帖 |
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G***G 发帖数: 16778 | 20 hi, I found a very strange phenomenum about goose
two weeks ago I saw one couple of geese hatching egg.
they were very alert when you got closer to them.
But today they were all gone, leaving five goose eggs without protection
in cold nest.
I have no incubator, so I cannot pick them up. also picking them in wild
is illegal.
I am afraid that the five egges can escape raccoon's nose.
so can someone tell use why and under what situations goose parent will
leave their eggs behind? |
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i******k 发帖数: 4625 | 21 坑爹的weather.com!
周五根据当时的天气预报决定周日去海钓,周六早上来到实验室setup好实验,顺手再
查查天气。NND降水概率变成周六有10%,周日倒是40%,打电话给buddy,他也觉得今天
可以出动。但我的实验已经箭在弦上,只好争分赶秒,12点做到最后一步4度
incubation,可以走人了,钓个鱼真不容易啊
赶到buddy家,一行四人向南进发,到达钓点开钓已经3点过了。本来这次是冲着tautog
去的,怎奈净是些少年儿童,鱼情不如2周前,用squid上了两条scup不表。5点过,
squid杆终于上正主了,18"fluke!正愁今年岸钓还没上过keeper fluke呢,没想到在
open season快结束时还搞到一条,值了
后来临近天黑时seabass来了,17.5一条外加撤退前缠线莫名其妙上的一条小些的,
buddy也上了两条大的
scup、fluke、seabass的open season就快陆续结束了,这应该是今年最后一次mixed
bag,聊以纪念
buddy的老爷子上toadfish一条,吞钩太深所以keep了,后来又嫌人长得难看,我就一
并收了吧
据... 阅读全帖 |
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m**k 发帖数: 18660 | 22 http://ajpendo.physiology.org/cgi/content/abstract/00354.2007v1?papetoc
减肥的人。虽然体重降低。但是脂肪酸氧化能力没有提高(还是和胖人一样,同瘦人差
远)
但是运动以后就明显提高.
Obesity is associated with a decrement in the ability of skeletal muscle to
oxidize lipid. The purpose of this investigation was to determine if
clinical interventions (weight loss, exercise training) could reverse the
impairment in fatty acid oxidation (FAO) evident in extremely obese
individuals. FAO was assessed by incubating skeletal muscle homogenates with
1-14C pal |
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e********3 发帖数: 18578 | 25 老板愿意花钱就行,你管的着吗?市场也需要incubate的好不好? |
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t******g 发帖数: 17520 | 26 Microsoft revealed its ambitious plans earlier this year to offer
interactive video content with the Xbox One. A live-action Halo TV series is
one of the first examples of Microsoft's promised efforts, but the company
appears to be working on a lot more. In an interview with Bloomberg,
Microsoft Studios vice president Phil Spencer has revealed that the Xbox
maker has "literally hundreds of ideas" for shows that they're incubating at
the moment.
Details of the Xbox TV shows have been lacking so f... 阅读全帖 |
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c**i 发帖数: 6973 | 27 Of course things can be made by craftsmen, maybe cheaper. But it does not
hurt to have new perspectives.
3D printing is new. Basically a printer receives input from you and lay down
powder of metal or ceramic or plastic (rather than the traditional ink
powder on paper) one layer at a time, which is consolidated by adding glue.
The next layer then starts. The whole process is pretty quick actually.
`````````
Nick Bilton, With 3-D Copier, Print Your Dishes. New York Times, May 16,
2011 (title and ... 阅读全帖 |
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a****a 发帖数: 26187 | 28 写的太好了,鼓掌!
你的文笔不错,标题改成后院生态链更恰当。
我今年还真的想垒鸡窝养上几只芦花鸡,连INCUBATOR都准备买一个了。有个小顾虑,
我只想养母鸡,等着吃有机蛋,那么如果是公鸡怎么办? 它老人家除了欺负母鸡,不
下蛋也就罢了,大清早的还要打鸣,吵到邻居怎么办?
不言童鞋知道如何辨别童年时候的公母鸡吗? |
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y********n 发帖数: 441 | 29 孵蛋器是Incubator, 网上输入这个词就能找到很多可以买的地方。
受精蛋到附近的养鸡养鸭的农场或者农户家里要,网上也可以买到。
也可以直接买小鸡。 |
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C********7 发帖数: 1035 | 30 看了这个版上相关的讨论,对于理解电影的含义非常有帮助。小弟有几点疑问希望大家
指点。
如前面的帖子说的,engineer的飞船里那些罐子里黑色的液体其实是异型幼虫的培养液
,刚开始的地质学家和生物学家就是被稍微长大的幼虫攻击,为什么生物学家没有能够
直接培养出异型?是incubation的时间不够长还是主体不够条件(必须要女的)?地质
学家后来在人类飞船前冒出来的时候是被异型控制了还是僵尸(因为他的面罩已经破碎
)?
还有一个困扰我的问题就是,为什么engineer要培养异型?很明显那个巨大的八抓鱼后
来也把engineer给整倒了然后用他孕育了第一代异型(不需要主体是女的?)。为什么
要把装满异型培养种子的飞船飞到地球?使用地球人培养异形有什么好处?整个地球都
变成异形的养殖场对于engineer有什么好处?
从后来的alien系列到aliens vs. predator系列来看,这个异型是有百害而无一利的。
另外,从一开始女主角用carbon reader读出的结果是engineer已经死了2000年了,也
就是说他们2000年前就想把飞船开到地球上?所以engineer醒过来的时... 阅读全帖 |
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C***l 发帖数: 2625 | 31 poor lady....totally lived in an incubator. |
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G********d 发帖数: 10250 | 32 【 以下文字转载自 NewYork 讨论区 】
发信人: IEDE (丫头,上馕), 信区: NewYork
标 题: 你用过或者听说过多少?。美国《时代周刊》50最佳发明
发信站: BBS 未名空间站 (Sat Nov 13 23:43:21 2010, 美东)
2010年美国《时代周刊》50最佳发明揭晓(全部榜单)
北京时间11月12日消息,据美国《时代周刊》报道,2010年《时代周刊》“50个最佳发
明”今日揭晓,涉及技术、交通、医疗健康、生物工程、绿色能源、服装、机器人与软
件以及军事等领域,苹果iPad、飞行汽车、喷气飞行包、可以杀死传播疟疾的蚊子的激
光器均上榜,这些成果都堪称今年科学、技术与艺术领域最重大的也是最酷的突破。
以下为全部榜单:
技术
* 1、苹果平板电脑iPad
* 2、移动定制杂志Flipboard
* 3、耳机式微型摄像机Looxcie
* 4、帮助实现创意梦想Kickstarter
* 5、自助刷卡系统Square
* 6、革命性的相机Sony Alpha A55 Camera
交通
* 7、美国铁路公司:用牛脂驱动的火车Amtrak's Beef-... 阅读全帖 |
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x5 发帖数: 27871 | 33 也就0.5M,大点的组也不稀罕哪
对了,你们那儿是不是就是干这个的?好象显微镜上架了个incubator |
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l*****e 发帖数: 8596 | 34 不懂医药,专门去google下
似乎是抗血栓的作用,但是在有意义的浓度下没有溶栓作用。
http://www.ncbi.nlm.nih.gov/pubmed/7135348
The effect of sodium ibuprofen on platelet activity in vivo and the
lysability of ex vivo thrombi was investigated. The formation of a
hemostatic platelet plug in the rabbit mesentery and platelet embolism as a
response to a laser-induced injury in the ear chamber of rabbits were used
as models for determining platelet activity. Ibuprofen at a dose of 25 mg/kg
i.v. was found to increase the primary (PHT) and t... 阅读全帖 |
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w*********g 发帖数: 10097 | 35 我5点就起来了
早上迷迷糊糊想起里昨晚走的时候忘了把培养皿放到incubator里就吓醒了,然后睡不
着就只好来实验室了 |
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d**********2 发帖数: 684 | 36 你要是连宋美龄讲演的最后那一句都理解不了,我看还是不要浪费时间了。
MITBBS关于宋的谑误太多。
为了忠于历史,我特意上传英文原版影音和英文稿。请注意这个稿子是宋亲自准备的,
是历史上第二个女性有机会在美国国会演讲,也是第一个中国人这样做。这个讲演有重
要的史学意义. 请别忘了,她当时是代表整个中国来作这个讲演的。在抗战的早期,她
多次去前线劳军,冒生命危险,死里逃生,数次受伤。她和努力和民国政府给当代的
PRC留下了许多历史遗产如:联合国发起国,联合国安理会常任理事国,许多世界一流
的科学家和艺术家,包括两弹元勋等。这些历史贡献是超越意识形态的。
这个演讲应该编入所有华人学校的英文教科书。
我个人最喜欢的一句宋美龄语录:We write our destiny; we become what we do.
片断:
录音全版:
2-1:
2-2:
讲演原文(全)
Addresses to the House of Representatives and to the Senate, February 18,
1943.
by Soong May-ling
Mr. Speaker an... 阅读全帖 |
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v******d 发帖数: 398 | 37 因为据Dr. Sheldon Copper讲,那个烘手机是细菌的incubator。 |
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n****c 发帖数: 78 | 38 好吧,铊溶液。
这里是一片用醋酸铊做体外神经-肌肉萎缩实验的文章。作者报道1mM到5uM的醋酸铊溶
液在体外处理大鼠神经-隔肌40分钟,即可导致细胞不可逆性功能丧失,
醋酸铊分子量263.43g/mole, 1mM到5uM相当于300mg/L到1.3mg/L。4磅暖水瓶容量1.8L
(google到的),那么2.4mg到540mg的量都可能导致症状出现。如果不是加在暖水瓶而
是喝水杯子的话,所需质量应该更少。
醋酸铊溶于水。鉴于下面所引文章可以用到1mM的工作浓度,按照一般实验室的经验,
储液浓度应该在100到1000倍,即100mM到1000mM,相当于30g/L到300g/L。这样,从
1000mM溶液取8uL到1.8mL,就能使暖瓶中醋酸铊的量达到2.4mg到540mg;从100mM液取
80uL到18mL,可以达到同样效果。
所以,使用铊盐溶液,同样可以达成目的。
Arch Toxicol. 1984 Mar;55(1):55-8.
The action of thallium acetate on neuromuscular transmission in the rat
p... 阅读全帖 |
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m*s 发帖数: 6855 | 39 "这里是一片用醋酸铊做体外神经-肌肉萎缩实验的文章。作者报道1mM到5uM的醋酸铊溶
---引用的试验是直接作用隔肌,把表层细胞浸泡在铊溶液了。而且又不知道隔肌的
厚薄,样品如何处理的。这样的实验室试验和人体铊中毒的实际情形差别也许比较大?
这儿是网上的东西,也不知道对不对。
http://baike.baidu.com/view/1725.htm
...所以,如果从体外对投与201Tl的患者进行放射线测量,能够映出保持了钠、钾、
ATP酶活性的心肌细胞的图像。这样201Tl作为诊查心肌梗死患者心肌细胞活性的放射性
医药品之一,已经被广泛用于临床。
另外,因为201Tl能够被癌吸收,也用于做癌的图像诊断。
而且,以为临床的铊的投药为痕迹量(低于100μg/g),所以不产生毒性作用。[1] (
日)桜井 弘.元素新发现:科学出版社,2006年
这儿100ug/g 实际是100ug/Kg (刊误)
致命剂量按照临床量的1000倍算的话:100ug/kg X 1000=100mg/kg; 体重50kg, 致命
量是100mg/kg=5g/50kg。 如果按照10000倍算的话,是50g/5... 阅读全帖 |
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t******n 发帖数: 2939 | 40 ☆─────────────────────────────────────☆
water123 (字符串) 于 (Thu May 2 18:12:51 2013, 美东) 提到:
为了实现诺言,我码了这篇长文,完全自带干粮义务劳动,要骂水军的请自己开贴。
我是从06年才看到这个案子,以前也没关注过,06年那会儿,我就没觉得指控孙维是凶
手的论断有多站得住脚,不能自圆其说的漏洞太多了,这么些年过去了,这个案子重热
,可惜除了炒剩饭,和某些人的想象力更进一步的把集体作案这种神论抛出来,我还没
看到任何比较有用的新的证据。
06年我就怀疑过是不是真的存在二次中毒,因为这个问题太关键了,其实朱令第一次中
毒期间,正忙于129排练,应该在寝室的时间是很少的,估计也就是回去睡个觉,外面
的人有更多的投毒机会。但是在没有证据的情况下,我选择相信专家,所以我的立论基
础是,在朱令返校后,发生了二次中毒。
每次看到朱令的近况,我也觉得很难受,当年下手的人,心肠得是多么狠毒啊,但有时
想想,如果这个人不是孙维,而是我们视线外的那个,她(他)真的可以生活得好吗,
朱令奇迹般一直顽强的活着,就是... 阅读全帖 |
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e******e 发帖数: 104 | 41 EVD is a severe acute viral illness often characterized by the sudden onset
of fever, intense weakness, muscle pain, headache and sore throat. This is
followed by vomiting, diarrhoea, rash, impaired kidney and liver function,
and in some cases, both internal and external bleeding. Laboratory findings
include low white blood cell and platelet counts and elevated liver enzymes.
People are infectious as long as their blood and secretions contain the
virus. Ebola virus was isolated from semen 61 day... 阅读全帖 |
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b****d 发帖数: 4648 | 42 that is what i used and am using...
10. Elute bound proteins by adding 90 μl 0.2 M glycine pH 2.5 (
incubation time: 60 sec under constant mixing) followed by separation.
Transfer the supernatant to a fresh tube and add 10 μl 1M Tris-base (pH 10.
4) for neutralization.
so .....could i use high ph pbs next time? instead of stupid tris?
tris is bad for mass spec |
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J******r 发帖数: 2806 | 43 据说今天是粽子节,昨晚和老娘电话的时候她还很关切的问我有没有吃到粽子。于是今
天专门去早茶找粽子,不过茶市里incubated融化到没有味道的粽子真的不能抚平某种
思念。思念那种味道,思念那种时光,思念每一点一滴的乡土和市井。
我很喜欢《海角一号》,到不是因为里面俗套的爱情,而是很多乡土的刻画,真的栩栩
如生。印象最深的是一场乡间的婚礼,全村人聚集在户外,放着咿咿呀呀的民间音乐,
各种猪头鸡腿不精致的食物“川流不息”,席间猜拳拼酒。没有什么高雅,却很是热闹
,很是生活。
我一直生活在象牙塔里,其实并没有很多生活的气息。于是我曾经有段时间特别特别的
迷恋生活。我甚至有点幻想能抛弃现有的所谓的well-educated life,跑到哪个特别原
汁原味的地方,过一点“接地气”的生活。也许三毛当时和我一样“突发奇想”了。只
不过她去撒哈拉生活了,我去山里休假看那里的人民生活了。如果下辈子我不识字会怎
样,生在一个小地方,不用漂泊来去,不用担心那么多国计民生风花雪月。我是不是会
像一个真正的“大妈”一样,不用考虑减肥健身,不用考虑怎么舒缓自己的情绪,不用
计划短期和长期的未来。我也许会耕种,会... 阅读全帖 |
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o******1 发帖数: 12177 | 44 北京时间11月12日消息,据美国《时代》周刊报道,2010年《时代》“50个最佳发明”
揭晓,涉及技术、交通、医疗健康、生物工程、绿色能源、服装、机器人与软件以及军
事等领域,苹果iPad、飞行汽车、喷气飞行包、可以杀死传播疟疾的蚊子的激光器均上
榜,这些成果都堪称今年科学、技术与艺术领域最重大的也是最酷的突破。
以下为全部榜单:
技术
* 1、苹果平板电脑iPad
* 2、移动定制杂志Flipboard
* 3、耳机式微型摄像机Looxcie
* 4、帮助实现创意梦想Kickstarter
* 5、自助刷卡系统Square
* 6、革命性的相机Sony Alpha A55 Camera
交通
* 7、美国铁路公司:用牛脂驱动的火车Amtrak's Beef-Powered Train
* 8、谷歌的无人驾驶汽车Google's Driverless Car
* 9、私人飞行背包Martin Jetpack
* 10、史上最轻巧的汽车设计Edison2
*11、 廉价家庭新概念汽车Antro Electric Car
* 12、电动车充电站Electric-Car Charging S... 阅读全帖 |
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F*********u 发帖数: 12190 | 45 Sheldon: Hot air blowers are incubators and spewers of bacteria and
pestilence. Frankly it would be more hygienic if they just had a plague
infested gibbon sneeze my hands dry. |
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H********g 发帖数: 43926 | 46 刚刚研究了一下乌骨鸡,wikipedia说乌骨鸡是禽类里母性最强的,经常被用来孵其它
禽类的蛋
Silkies lay a fair number of cream-colored eggs, but production is often
interrupted due to their extreme tendency to go broody; a hen will produce
100 eggs in an ideal year. Their capacity for incubation, which has been
selectively bred out of most fowl bred especially for egg production, is
often exploited by poultry keepers by allowing Silkies to raise the
offspring of other birds. In addition to being good mothers, Silkies are
universally renow... 阅读全帖 |
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g**a 发帖数: 2129 | 47 incubation period. 可能已经感染病毒,病毒已经进入细胞,但是还没有开始大规模
扩散。这段时间验血好像查不出来。测序理论上可以,实际操作上根本来不及。 |
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