c*****t
发帖数: 1879 | 1 我有一笨问题:
ATP hydrolysis 出的能量是怎么样产生的? 这里要的不是简单的 equation,
我这里指的是:
当一 ATPase (比如 MT motor proteins) hydrolyze ATP 时, 可以
想象 ATP 和该 ATPase 总是大约处于一定的位置, 那么反应的能量是以什么
形式放在什么原子上, 处于什么方向? 该原子又如何把能量传递? 是否一般
ATPase 的能量传递 (初始) 原理相象?
有什么书/Review paper 么? |
|
G***a
发帖数: 27294 | 2 好的话，我就去听了, 这是Vice Chancellor invite的讲座。。。 suppose是good
research
Erin E. Kershaw, MD, assistant professor of medicine, School of Medicine
“Lipolysis in Metabolic Syndrome: Beyond Adipose Tissue.”
Topic Overview:
Obesity and metabolic syndrome are global health threats. Insulin resistance
and metabolic complications of obesity are strongly associated with
intracellular accumulation of triacylglycerol (TG); however, the mechanisms
underlying TG accumulation and its relationship to lipid-induced tox... 阅读全帖 |
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O**********0
发帖数: 61 | 3 ☆─────────────────────────────────────☆
BHistory (往者的影子) 于 (Fri Sep 9 00:20:11 2011, 美东) 提到:
俺曾认为生命是进化起源的，后来越找越发现进化论像是信仰而非科学结论。可是每有
飞机网友觉着俺如此看待进化论是反科学了。
所以俺本着科学的精神，问个问题，求各位飞机进化论牛人给个科学的答案。
大家知道达尔文进化论是以遗传机制为基础的，第一个有遗传机制的生命怎么来的，当
时根本没有提及。
后人发现这一问题后，就又提出了化学进化论，就是无机分子在某种机制下，自动组合
出了第一个有遗传的生命。此后达尔文进化论似乎可以take care（其实未必，今后再
讨论）。
今天我们先看比第一个有遗传生命的出现简单万亿倍的问题，第一个蛋白质如何化学进化出
现？
先亮亮俺一个愚昧基督徒的愚见：
已知最简单的具有遗传机制的单细胞生命的复杂度已经远远超出人们最初的想象，大概
有400种蛋白质几十万个组成。其中较小的蛋白质大概100个氨基酸，如果在随机热运动
中组成，因为自然界对称的氨基酸不少于40种（20多种氨基酸及... 阅读全帖 |
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O******y
发帖数: 110 | 4 关键词 hydrolysis mechanism， kinetic analysis， COS hydrolysis
journal of Chemistry |
|
F***I
发帖数: 4170 | 5 Monosodium glutamate
From Wikipedia, the free encyclopedia
Jump to: navigation, search
This article is about the chemical compound. For its use in food, see
glutamic acid (flavor). For glutamic acid in general, see glutamic acid.
Monosodium glutamate
Chemical composition of monosodium glutamate
Structure of monosodium glutamate
Crystalline monosodium glutamate
IUPAC name[hide]
Sodium 2-Aminopentanedioate
Identifiers
CAS number 142-47-2 Yes
PubChem 85314
ChemSpider 76943 Yes
UNII ... 阅读全帖 |
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j*****9
发帖数: 716 | 6 http://scienceblogs.com/webeasties/2010/12/guest_post_arsenate-based_dna.php
In the wake of the NASA excitement over the new arsenic study, and my
promise to give a detailed review of the paper itself, I have recruited a
colleague with strong opinons about the work, a solid chemistry and
microbiology background, and "Dr." in front of his name to share his
analysis. I will be posting have posted my personal and less-technical take
on the whole thing soon, so stay tuned as well.
Dr. Alex Bradley u... 阅读全帖 |
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l******g
发帖数: 1623 | 7 想过是不是hydrolysis, 我感觉自发hydrolysis是比较慢的反应, 今天作了个试验, 把
蛋白从pH8.5 稀释到pH4的buffer里, 5分钟内所有蛋白都变成truncated了 |
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d****i
发帖数: 360 | 8 E-mail: x**********[email protected]
A kinetic and mechanistic study of the hydrolysis of camptothecin and some
analogues
Author: A kinetic and mechanistic study of the hydrolysis of camptothecin
and some analogues Fassberg, J.
Journal: Journal of pharmaceutical sciences
ISSN: 0022-3549 Date: 1992
Volume: 81 Issue: 7 Page: 676 |
|
q****e
发帖数: 3660 | 9 1. Alkaline hydrolysis of polyacrylonitrile. On the reaction mechanism
Litmanovich, Arkady D.; Plate, Nicolai A.
Macromolecular Chemistry and Physics (2000), 201(16), 2176-2180.
2. Acidic Hydrolysis of Polyacrylonitrile: Effect of Neighboring
Groups
Liya B. Krentsel , Yaroslav V. Kudryavtsev , Alexandr I. Rebrov , Arkady D.
Litmanovich , and Nicolai A. Platé
Macromolecules, 2001, 34 (16), pp 5607–5610
please send them to [email protected]
(function(){try{var s,a,i,j,r,c,l,b=document.get... 阅读全帖 |
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c***s
发帖数: 70028 | 10 平安(资料图)
杨坤(资料图)
《中国好声音》虽然已经结束，但关于节目的争议并未终止，评委杨坤由于遭到网友近20万余条的围攻被迫关闭评论，并表示自己想当岳飞，但也背得起秦桧的骂名。平安则在微博力撑导师，称十分珍惜和导师之间的情谊，谢谢杨坤让自己在好声音上起飞。
杨坤被网友质疑过分袒护学员金志文和关喆，而他与丁丁的关系也遭网友质疑，尽管当事人作出了回应，但仍难平息网友的猜测。很多网友也来到杨坤的微博对他进行攻击。杨坤表示，“人人都想当岳飞，我杨坤更想当，而且从未改变过，可一不留神被污蔑成秦桧，伤心是肯定的，突然觉得如此渺小，如此无力，甚至连想替自己说句话的能力都没有了，不过我可以面对，也必须面对，我既然想做岳飞，就一定能背得起被秦桧这个骂名。”自从在9月30日下午，《中国好声音》决赛前发出微博之后，这条微博已经被转发了将近十万，评论超过六万。
也许是网友的谩骂让杨坤觉得不适应，他已经关闭了微博的评论。而杨坤最近的一条微博是10月2日凌晨1点发出的，内容是，“祝我们内蒙唯一的摇滚音乐节(安达音乐节)越办越成功！
新闻链接
杨坤力挺金志文被疑有黑幕 平安人气高却遭淘汰
《中国好声音》导师终极... 阅读全帖 |
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r******i
发帖数: 1445 | 11 这个怎么样？
Recent researches on heavy water
By Chang, Tsing-Lien
From Science (Washington, DC, United States) (1944), 100, 29-30. Language:
Unavailable, Database: CAPLUS
Work done in Free China since 1937 is summarized (cf. C. A. 34, 306.3; 35,
5008.9, 50228). The molal f.-p. lowering of D2O with acetone as solute is 2
.00° as compared with the calcd. value of 2.004°. Expts. on the reduction
of permanganate by H2O2 showed that the autocatalysis of Mn++ ion proceeds
more quickly in the presence of ... 阅读全帖 |
|
发帖数: 1 | 12 胡小健
基本信息
职称：副教授
电话：021-31246545
邮箱：[email protected]
地址：上海市杨浦区复旦大学新江湾校区生命科学大楼A201-5室
个人简介
1988-1992年就读于复旦大学生理学和生物物理学系并获学士学位，1992-1994年于武汉
大学医学院开始大学教师生涯，1994-2000年在复旦大学生理学和生物物理学系攻读研
究生并于2000年3月获理学博士学位，之后留校工作并于2008年晋升副教授。2001-2004
年在美国国立健康研究院从事博士后研究（导师：Wei Yang）。擅长于蛋白质晶体结构
技术和包涵体蛋白的可溶化技术。
主要研究方向 (Research Interests)
用蛋白质结构生物学、生物化学和生物物理学等手段， 研究蛋白质、酶的结构与功能
关系、相互作用的分子机制等。研究方向为：1)乙型肝炎病毒HBx蛋白的结构与功能。
实验室在2015年对该蛋白的可溶性研究有突破，首先将一个HBx的核心截断体可溶化得
到有功能的单体。之后优化相关技术可以获得高纯度、高稳定性的HBx核心截断体，
2018年初开始申请与该HBx核心截... 阅读全帖 |
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m****s
发帖数: 18160 | 13 【 以下文字转载自 Biology 讨论区 】
发信人: niceboat (Spore), 信区: Biology
标 题: Open Position: Research Scientist – Metabolic Engineering & Fermentation
发信站: BBS 未名空间站 (Fri Nov 15 16:01:59 2013, 美东)
Job ID: GF2013A003
Posting Title: Research Scientist – Metabolic Engineering & Fermentation
Brief Introduction of Gate Fuels Inc.: “Imagination, Innovation,
Implementation --- Enabled Technologies for a Green, Clean World”
Gate Fuels, Inc. is a biotechnology startup company that is developing
recombinant Bacillus s... 阅读全帖 |
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p*******r
发帖数: 156 | 14 Postdoctoral positions available
A postdoctoral position is available in the Laboratory of Cellular and
Molecular Biology at the National Cancer Institute. A focus of current
studies is understanding the contribution of integrin adhesion complexes,
including focal adhesions and invadopodia, to the control of cancer cell
proliferation and survival. A class of proteins called the Arf GTPase-
activating proteins (Arf GAPs), which regulate the Ras-superfamily proteins
Arfs and integrin adhesions, ... 阅读全帖 |
|
s********1
发帖数: 27 | 15 Research Fellow/Senior Research Fellow
Job responsibilities:
Full time commitment to the project
Catalyst screening/preparation for hydrogenation and hydrolysis /
dehydrogenation
Reaction thermodynamics, kinetics, pathways/mechanistic investigations
Develop new catalytic reactive separation process
Requirements:
Ph. D in catalysis or related fields
Postdoctoral experience in an overseas research establishment or
university
Broad expertise in hydrogenation/hyd |
|
m****s
发帖数: 18160 | 16 【 以下文字转载自 Biology 讨论区 】
发信人: niceboat (Spore), 信区: Biology
标 题: Open Position: Research Scientist – Metabolic Engineering & Fermentation
发信站: BBS 未名空间站 (Fri Nov 15 16:01:59 2013, 美东)
Job ID: GF2013A003
Posting Title: Research Scientist – Metabolic Engineering & Fermentation
Brief Introduction of Gate Fuels Inc.: “Imagination, Innovation,
Implementation --- Enabled Technologies for a Green, Clean World”
Gate Fuels, Inc. is a biotechnology startup company that is developing
recombinant Bacillus s... 阅读全帖 |
|
p*******r
发帖数: 156 | 17 Postdoctoral positions available
A postdoctoral position is available in the Laboratory of Cellular and
Molecular Biology at the National Cancer Institute. A focus of current
studies is understanding the contribution of integrin adhesion complexes,
including focal adhesions and invadopodia, to the control of cancer cell
proliferation and survival. A class of proteins called the Arf GTPase-
activating proteins (Arf GAPs), which regulate the Ras-superfamily proteins
Arfs and integrin adhesions, ... 阅读全帖 |
|
s*******s
发帖数: 623 | 18 Job ID: GF2011A001
Posting Title: Research Scientist/Research Associate/Bacterial Geneticist
Brief Introduction of Gate Fuels Inc.: “Imagination, Innovation,
Implementation --- Enabled Technologies for a Green Clean World”
Gate Fuels Inc. is a research start-up firm based in Blacksburg, VA, with
the goal of developing microbial and cell-free platform technologies that
enable low-cost production of biochemicals and biofuels. The first platform
is based on industrially-safe Bacillus subtilis. Thi... 阅读全帖 |
|
p*******r
发帖数: 156 | 19 Postdoctoral positions available
A postdoctoral position is available in the Laboratory of Cellular and
Molecular Biology at the National Cancer Institute. A focus of current
studies is understanding the contribution of integrin adhesion complexes,
including focal adhesions and invadopodia, to the control of cancer cell
proliferation and survival. A class of proteins called the Arf GTPase-
activating proteins (Arf GAPs), which regulate the Ras-superfamily proteins
Arfs and integrin adhesions, ... 阅读全帖 |
|
a*****r
发帖数: 127 | 20 http://en.wikipedia.org/wiki/Aspartame
Chemistry
Aspartame is the methyl ester of the dipeptide of the natural amino acids L-
aspartic acid and L-phenylalanine. Under strongly acidic or alkaline
conditions, aspartame may generate methanol（甲醇） by hydrolysis. Under
more severe conditions, the peptide bonds are also hydrolyzed, resulting in
the free amino acids.[4]
For some markets, aspartame is manufactured from phenylalanine produced by a
genetically modified strain of E. coli,[5][6] a bacterium |
|
p*****r
发帖数: 805 | 21 Elsevier的材料类杂志， IF~2.3左右。
关键词：Mesoporous silica，high specific surface area，calcinations, XRF,
TGA, hydrolysis-aging,trimethylmethoxysilane,glycerol.
有意者请站内联系，先到先得，包括你的name，email/phone/fax(学校或单位的）,
list of publications（包括文章的title）给我, 如果背景合适我会向杂志编辑推荐
。如果你得到了审稿机会，希望秉持专业精神按时保质保量完成。 |
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l****5
发帖数: 2968 | 22 包子酬谢
轻发g***********[email protected]
1) Bartolomé, B., Santos, M., Jiménez, J. J., Del Nozal, M. J., & Gómez-
Cordovés, C. (2002). Pentoses and hydroxycinnamic acids in brewer's spent
grain. Journal of Cereal Science, 36(1), 51-58.
2) Heinzelmann, K., & Franke, K. (1999). Using freezing and drying
techniques of emulsions for the microencapsulation of fish oil to improve
oxidation stability.Colloids and Surfaces B: Biointerfaces, 12(3), 223-229.
3) Gao, M. T., Hirata, M., Toorisaka, E., & Hano, T. (200... 阅读全帖 |
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s***r
发帖数: 4013 | 23 收到5封。。大部分都做分子的，我收的稿子最好是做下游的pretreatment hydrolysis
啥，不过我都存下了，以后有相关的会推荐各位。谢谢！ |
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l******t
发帖数: 12659 | 24 u r so nice!
hydrolysis |
|
|
B******1
发帖数: 9094 | 26 TJ 在英特尔科学奖半决赛入围学生里又牛了一把！
Thomas Jefferson High School for Science and Technology
Brenner, Alec, 17, McLean
Viscoelastic Modeling of Tidal Heating in Terrestrial Exoplanets
Gondi, Suhas, 17, Centreville
Investigating microRNA-Mediated Regulation of Class Specific Dendrite
Morphogenesis
Ho, Katherine, 17, McLean
Real Time Apoptosis Imaging by an EB Conjugated Caspase 3 Activatable Probe
Peng, Jennifer, 17, Oak Hill
Investigating Rhodopsin Organization in Native and Model Membranes by Atomic
Force ... 阅读全帖 |
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B******1
发帖数: 9094 | 27 你说的太对了。东边的教学环境比西边好。因为经济的原因，学区的侧重点和可选项都
会不同。TJ 高中就是一个范例。
TJ 在英特尔科学奖半决赛入围学生里又牛了一把！
Thomas Jefferson High School for Science and Technology
Brenner, Alec, 17, McLean
Viscoelastic Modeling of Tidal Heating in Terrestrial Exoplanets
Gondi, Suhas, 17, Centreville
Investigating microRNA-Mediated Regulation of Class Specific Dendrite
Morphogenesis
Ho, Katherine, 17, McLean
Real Time Apoptosis Imaging by an EB Conjugated Caspase 3 Activatable Probe
Peng, Jennifer, 17, Oak Hill
Investigating Rhodopsin Org... 阅读全帖 |
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J***n
发帖数: 210 | 28
感谢万能的GOOGLE,找到了他家“鸡胸”的原料：
http://subway.com/subwayroot/MenuNutrition/Nutrition/frmUSIngre
px
OVEN ROASTED CHICKEN PATTY：
-Oven roasted chicken with rib meat, water,
-seasoning (corn syrup solids, vinegar powder [maltodextrin, modified
corn starch & tapioca starch, dried vinegar], brown sugar, salt,
dextrose, garlic powder, onion powder, chicken type flavor [hydrolyzed
corn gluten, autolyzed yeast extract, thiamine hydrochloride, disodium
inosinate & disodium guanylate]),
-sodium phosphate.
看来不算... 阅读全帖 |
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z********0
发帖数: 9013 | 29 ☆─────────────────────────────────────☆
littlesu (小苏夫妇的共用ID) 于 (Mon Mar 21 20:54:41 2011, 美东) 提到:
谁能给个蛋白质伤肾的权威一些的联接啊？是science还是myth？
我不吃蛋白粉，但每天的protein也在200g上下，主要来源是肉蛋奶和豆腐，我喝水多
（习惯），吃蔬菜也多。。。没有什么不适啊？
☆─────────────────────────────────────☆
dai (百合开过·风烛残年) 于 (Mon Mar 21 20:56:16 2011, 美东) 提到:
正常人一天200g啥事没有。
肾功能不全的人要限制摄入蛋白质。
☆─────────────────────────────────────☆
Rounin (旅) 于 (Mon Mar 21 20:57:16 2011, 美东) 提到:
说明你肾本来就好
吃进去的氨基酸不可能全部被同化，剩下的氨基酸只能分解掉，产生的又都是含氮废物
，肾
的负担肯定会加重的
☆──────────... 阅读全帖 |
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a**********e
发帖数: 418 | 30 "即使按3kcal/mol算，25度的K大约是0.3"
3 Kcal = 12.5 KJ, greater than 99%.
"随着肽链增
变得更稳定Only if a peptide reaches certain length of chain, which have
tertiary or higher structures. Let's say 100 amino acids only makes one
dipeptide at equilibrium based on 3 cal/mol. the chance to make a random
tripeptide is 1 out of 1000. How many amino acids are required to make a
RANDOM peptide of greater than 50 amino acids? you do the calculation.
要考虑能垒的话，多肽水解也不是什么容易发生的过程。
The activation energy for hyrolysis is lower t... 阅读全帖 |
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a**********e
发帖数: 418 | 31 同意LZ的概率计算是建立在错误的基础上的
Water is one of the major components on earth. Formation of peptides should
not happen under these conditions. The hydrolysis of the peptides, if by any
chance formed, will dominate. If there were any "natural" amino acids ever
existed, they had to be monomers. I highly doubt there would be a chance to
form a di or tri peptide by nature, not mentioning the complicated
polypeptide structure. |
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t*******d
发帖数: 2570 | 32 “The hydrolysis of the peptides, if by any chance formed, will dominate. ”
这是怎么得出来的？
should
any
ever
to |
|
a**********e
发帖数: 418 | 33 http://en.wikipedia.org/wiki/Peptide_bond
A peptide bond can be broken by amide hydrolysis (the adding of water). The
peptide bonds in proteins are metastable, meaning that in the presence of
water they will break spontaneously, releasing 2-4 kcal/mol [1] of free
energy, but this process is extremely slow.
consider the equilibrium reaction at rt: dipeptide + H2O ---2 amino acids
The free energy difference is 2-4 K/Cal, I am taking the average 3, which
correspond to a greater than 99% of the prod... 阅读全帖 |
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f*******c
发帖数: 94 | 34 you are right, DOE is not idiots, but this pathway can only work if
follwoing problem can be fixed:
1) a energy efficient pretreatment method to handle >30% biomass and convert
all cellulose + hemicellulose to simple sugars (glucose, fructose, sucrose
and xylose), those methods include solid acid catalysis, hydrothermo
hydrolysis or other advanced tech.
2) fast and energy saving separation method to remove lignin from biomass
hydrolysate
3) energy saving separation method for ethanol purificatio |
|
r******0
发帖数: 2753 | 35 碳酸氢盐: bicarbonate
碳酸盐: carbonate
碳酸: Carbonic acid
水解: hydrolysis
电离: electrolysis
All these words and detailed explanation are available in wiki. |
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M****e
发帖数: 70 | 36 cystal of ice will form that can mechanically shear the protein.
the same as DNA. for storage of DNA, it is better to be stored
in buffer to avoid such mechanical shearing and hydrolysis,
if you concern about EDTA, you can use Tris-HCl (pH 8.0).
Here is some general guide for storage of purified proteins:
For short term storage (up to 24 h), most proteins can be kept
at 4C. For storage times longer than 24 h r at 4C, it may be
necessary to filter sterilize the protein preparation (through
a 0.22 |
|
s******y
发帖数: 28562 | 37 The most complete way is hydrolysis in basic buffer and heating. |
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h*****t
发帖数: 1226 | 38 Why use enzyme, using hydrolysis, chemical treatment is much faster. |
|
m******i
发帖数: 73 | 39 I need to detect some modifications on lysine. But the compound is not very
stable, which can not survive the harsh condition used for acid hydrolysis.
So, I have to use mild condition like enzyme digestion. |
|
y*****6
发帖数: 47 | 40 Title: Invited review: The use of distillers products in dairy cattle
diets
Source: Journal of Dairy Science [0022-0302] Schingoethe yr:2009 vol:92
iss:12
pg:5802 -5813
Title: Hydrolysis and fermentation of pericarp and endosperm fibers recovered from
enzymatic corn dry-grind process
Source: Cereal chemistry [0009-0352] Dien yr:2005 vol:82 iss:5 pg:616 -620
信箱：j*****[email protected]
多谢 |
|
b******n
发帖数: 82 | 41 http://www.cell.com/abstract/S0092-8674%2810%2901180-3
The ER UDPase ENTPD5 Promotes Protein N-Glycosylation, the Warburg Effect,
and Proliferation in the PTEN Pathway
Min Fang, Zhirong Shen, Song Huang, Liping Zhao, She Chen, Tak W. Mak,
Xiaodong Wang
* Highlights
* The ER UDPase ENTPD5 is upregulated by AKT in PTEN mutant cells
* ENTPD5 hydrolyzes UDP to UMP to promote protein N-glycosylation
* ENTPD5, along with CMPK1 and AK-1, hydrolyze ATP to AMP, increasing
aerobic glycolys... 阅读全帖 |
|
r****i
发帖数: 323 | 42 J Recept Signal Transduct Res. 2010 Dec;30(6):372-5. Epub 2010 Aug 23.
On the roles of Mg in the activation of G proteins.
Birnbaumer L, Zurita AR.
Laboratory of Neurobiology, National Institute of Environmental Health
Sciences, NIH, DHHS, Research Triangle Park, NC27709, USA. birnbau1@niehs.
nih.gov
Abstract
In this review, we highlight the evolution of our knowledge about the way Mg
(2+) participates in the activation of heterotrimeric G proteins, beginning
with its requirement in hormonal sti... 阅读全帖 |
|
|
s*******s
发帖数: 623 | 44 Job ID: GF2011A001
Posting Title: Research Scientist/Research Associate/Bacterial Geneticist
Brief Introduction of Gate Fuels Inc.: “Imagination, Innovation,
Implementation --- Enabled Technologies for a Green Clean World”
Gate Fuels Inc. is a research start-up firm based in Blacksburg, VA, with
the goal of developing microbial and cell-free platform technologies that
enable low-cost production of biochemicals and biofuels. The first platform
is based on industrially-safe Bacillus subtilis. Thi... 阅读全帖 |
|
A******y
发帖数: 2041 | 45 What are you talking about? Chemically,RNA is not stable because of the
hydroxy group (self hydrolysis)...in his/her situation the RNA is dry, so I
think it will still be good. |
|
l**********1
发帖数: 5204 | 46 GTP→GDP already
please go to new book
"New Insights into the Mechanisms
of Cytomotive Actin and Tubulin
Filaments" 2011 Elsevier Inc.
by Christopher H.S. Aylett, Jan Lo¨we, and Linda A. Amos
full text link:
//www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/AmosReview2011.pdf
its pp20
On phosphate release, Wang and Nogales proposed that the contact
between T7 and the nucleotide of the adjacent subunit within the E-site
interface becomes disrupted, leaving contacts only on the side of the
subunit–subunit ... 阅读全帖 |
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s****n
发帖数: 234 | 47 This is how we make home-made Halo-Atto647N in our lab using amine/NHS ester
chemistry. It is probably not the most cost-efficient way, but we did get
huge amount of Halo-Atto647N. Hope this would help.
Reagents and Reaction Set Up:
Dissolve 5mg of Atto NHS ester in 400ul of Anhydrous N,N DMF. (Final
Concentration 10mM)
Add 120 ul of 100mM stock solution of Amino HaloTag Ligand. (Final
Concentration 20mM)
Add 3.1 ul of DIPEA (reagent grade) to the solution. (Final Concentration
30mM)
Bring final... 阅读全帖 |
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g***y
发帖数: 205 | 48 1. HK dephosphorylate response regulator的时候，phosphate去哪了？
hk hydrolysis the RR~P, free P就到环境中去了 （可以去TLC 去测？）
还是说 先生称 HK~P， 然后 HK~P 自己dephosphorylation了，free P 去环境中了。
2.in vitro phosphorylation实验中，一个分子量很大的蛋白，能促进HK 的
dephosphorylation，但是不影响 phosphortransfer to RR. 这怎么解释啊？
除了conformation change这种万能解释。 |
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s*****g
发帖数: 7857 | 49 是Glucosamine吗?常见的关节保健品.
常是三种合一Glucosamine + Chondroitin + MSM
Glucosamine (C6H13NO5) is an amino sugar and a prominent precursor in the
biochemical synthesis of glycosylated proteins and lipids. Glucosamine is
part of the structure of the polysaccharides chitosan and chitin, which
compose the exoskeletons of crustaceans and other arthropods, cell walls in
fungi and many higher organisms. Glucosamine is one of the most abundant
monosaccharides.[1] It is produced commercially by the hydrolysis of
... 阅读全帖 |
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D*******r
发帖数: 162 | 50 papers are as below:
1.Stability of Vancomycin Hydrochloride Solutions at Various PH Values as
Determined by High-Performance Liquid Chromatography
Drug Development and Industrial Pharmacy ,1995, Vol. 21, No. 2 , Pages 257-
264 (doi:10.3109/03639049509048108)
2.Pharmaceutical Research
February 1987, Volume 4, Issue 1, pp 42-45
Stability of Lidocaine in Aqueous Solution: Effect of Temperature, pH,
Buffer, and Metal Ions on Amide Hydrolysis
please send to m*********[email protected]
Thanks! |
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