h****g
发帖数: 439 | 1 我们都是用hetero的。我想hetero产生的cre足以切掉loxp吧，而且hetero好mating，
pups会有一半对照。 |
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G******O
发帖数: 189 | 2 Homo和hetero 在功能上应该是差不多的,但是通常我们用Hetero的原因除了能得到Wild
-Type 对照外,一个重要原因是减少了出现假阳性的PHENOTYPE的几率.因为一般Cre是转
基因进去的，不确定Cre的具体染色体定位，假如碰巧和某基因在相同的位点，HOMO会
带来无关性状。
这度【 在 htscorpion (snoopy) 的大作中提到: 】 |
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r***e
发帖数: 2539 | 3 我想绝大多数情况下hetero足够了，但我做过一个primary cell culture subclone，大
多数细胞是-/-,但的确发现一些是flox/-，就是说切了一个allele，应该和cre promot
er的强度有关。 |
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c****e
发帖数: 1842 | 4 忽视掉的一点是，更适宜的比较指标，应该是hetero和homo relationship 分手率，而
不是对比hetero family divorce rate和homo breakup rate。hetero恋爱分手率不会低
于homo。
另外，hetero和homo两个sample size 也是不同的，虽然这里没有self-select问题。 |
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M******g
发帖数: 152 | 5 I got a wierd genotyping result:
the gene I'm working on is arrb1.
breeding pair: arrb1 hetero + CreERt2 homo,
I'm expecting some pups will be arrb1 hetero/CreERt2 hetero.
However I didn't get the above genotype.
What I got is arrb1 heter/CreERt2 homo. I don't why I can get CreERt2 homo
in this generation.
I have used different primers/PCR, and they all gave me the same result:
arrb1 hetero/CreERt2 homo.
This is most wierd genotyping I've ever met. I don't understand
Mouse expert: please ad... 阅读全帖 |
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p***i
发帖数: 96 | 6 我用的是CAMKII Cre 老鼠与我的flox老鼠杂交，希望只在forbrain区knockdown我的
gene. 目前得到了Cre Hetero/flox hetero还有Cre homo/flox hetero.
请问如何检测knock down efficiency呢？
1. take forbrain, extract genome, RT PCR
2. perfuse brain, immunostain brain sections with antibody against my gene
of interest. Can I costain with anti Cre antibody? If so, where can I buy a
good one?
3. DAB staining brain sections using antibody against my gene of interest.
请问一般Cre 的knock down efficiency 是多高？ Cre homo or hetero 会有很大区别
吗？
谢谢 |
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g********d
发帖数: 4174 | 7 Results
Top of page
Abstract
Introduction
Materials and methods
Results
Discussion
Conclusion
Acknowledgements
References
Review of epidemiological data from donors and other sources
The prevalence of anti-HIV in blood donors is very low. Both the number and
the proportion of anti-HIV-positive donors, reported as MSM, have fallen
over the years (Fig. 1). However, this route of infection still constituted
approximately 27% of anti-HIV-positive donations during 1998–2000. Sexual
intercourse betwee... 阅读全帖 |
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m******1
发帖数: 19713 | 8 如果CU和marriage有完全一样的权利了，那还要CU干什么？
那些只想登记CU而不想登记结婚的异性恋伴侣们怎么办？
难道再定义一套hetero-CU和一套homo-CU？
hetero-CU=regular CU？
homo-CU=gay marriage？
然后，异性恋伴侣们就说了：为什么要让我们的CU改名字？我们本来就叫CU，而同性伴
侣的CU就应该叫gay CU。
然后政府就说了，既然gay CU的权利其实和marriage一模一样，不如把gay CU改叫gay
marriage更合适。。。
于是，皆大欢喜，绕了一圈，又回到原地。
这不是脱了裤子放P么？ |
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h*******g
发帖数: 2201 | 9 No, I will stay far away from CA as possible. : )
I hope some of your gay friends are more sensible. I am here, try to learn
what caused gay, and I am not against gay as I laid it out at my first post(
support gay couples get equal financial benefits as hetero couples). But
some of the IDs goes directly to attack me on aspects has nothing to do with
this discussion and become ridiculous, such as express explicit hatred
against all structural biologists, I wonder: what the heck is wrong with
suc... 阅读全帖 |
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l****z
发帖数: 29846 | 10 by Mike Piccione
01/04/2011
Here is a sampling of the “Don’t Ask, Don’t Tell” responses I received
from my editorial last week. There was only one nasty name calling liberal
and I challenged him to write an article expressing his point. He has failed
to respond with nothing but name calling.
Mike P.
Army Warrant Officer
I enlisted in 1964 and retired in 2006. This gives me a fair amount of
exposure to military life including multiple tours in RVN, Korea, Japan and
the Philippines.(Purple Hearts... 阅读全帖 |
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j**4
发帖数: 10425 | 11 上网看看， 很多评论canada杀人案， 都拿住gay 这一点不放。。。 拜托, the same
thing can easily happen on hetero sexual ppl! the thing we need to learn is
never go into a stranger's house and have sex! no matter it's homo sex or
hetero sex!
mitbbs上的网民们， 底下搞ONS的， 都要当心了
masturbation sometimes not only saves u from sexual frustation, it may save
ur life as well |
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b*******n
发帖数: 26 | 12 //support一把. I always think homo is better than hetero(right one?). I feel
homo is more 自发, HETERO IS MOSTLY 自觉的. so homo is more real in a sense.
or you won't be willing to pay so much so be in a homo relationship. |
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h********n
发帖数: 4079 | 13 我昨天在想这个Cre的问题。 我想， 能不能用这个transgene头围的序列设计primer测
序， 测出transgene前后的序列， 然后设计primer根据区分homo hetero and wt.
homo和wt都是一条带， hetero两条带。
cre/
pay
you
the
cre,
different |
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D*a
发帖数: 6830 | 14 你genotyping判断CreERt2 homo还是hetero的依据是什么？
有的primer只能判断存在基因插入还是不存在基因插入，只有纯wt的时候是此条带缺失
的，hetero和homo有一样的带。 |
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s*********g
发帖数: 68 | 15 how to know if the Cre is homo or hetero. My understanding is that Cre is
randomly inserted in genome even potentially with multiple copies.
Is really a way to know if it is hetero or homo? |
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D*a
发帖数: 6830 | 16 哦对，我想错了。
hetero可以再详细分么？比如如果是A/C,按照测序原理会不会看出来是爸爸是A还是妈
妈是A？还是只能看出来是hetero呢？
X
SNP |
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s*****r
发帖数: 223 | 17 又来学习请教了， 据说这是一个很经典的题目
suppose that in each individual of a large population there is a pair of
genes, each of which can be either x or X, that controls eye color: those
with xx have blue eyes, while heterozygotes (those with Xx or xX) and those
with XX have brown eyes. The proportion of blue-eyed individuals is p2 and
of heterozygotes is 2p(1−p), where 0
its own genes to the child; if a parent is a heterozygote, the probability
that it transmits the gene o... 阅读全帖 |
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f**d
发帖数: 768 | 18 这是一本计算神经科学的优秀著作，全文拷贝这里（图和公式缺），有兴趣的同学可以
阅读
如需要，我可以分享PDF文件（－－仅供个人学习，无商业用途）
From Computer to Brain
William W. Lytton
From Computer to Brain
Foundations of Computational Neuroscience
Springer
William W. Lytton, M.D.
Associate Professor, State University of New York, Downstato, Brooklyn, NY
Visiting Associate Professor, University of Wisconsin, Madison
Visiting Associate Professor, Polytechnic University, Brooklyn, NY
Staff Neurologist., Kings County Hospital, Brooklyn, NY
In From Computer to Brain: ... 阅读全帖 |
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f****l
发帖数: 8042 | 19 WIKI
This is a list of notable Chinese Americans, including both original
immigrants who obtained American citizenship and their American
descendants.
These are people who have made significant contributions to the American
culture or society politically, artistically or scientifically, or have
appeared in the news numerous times.
To be included in this list, the person must have a Wikipedia article
showing they are Chinese American or must have references showing they
are Chinese American and a... 阅读全帖 |
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G*****7
发帖数: 1759 | 20 不涉及gay的立法也不是一蹴而就的，渐渐修改到今天的体系嘛。你数数宪法等等的修
正案。
先修改到homo/hetero都接受的一个起点，然后在根据两方面政法势力的角逐，逐步改
善阿。
如果够民主，科学的观察、政策研究，到时候都有发言权。 |
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G*****7
发帖数: 1759 | 21 那他们要搞跨国绑架收养了。
gay虽然性生活和hetero不一样，在对待后代方面内心还是差不多的，
你看看纽约好莱坞有头有脸的gay，哪个不是收养了孩子当宝贝一样的。 |
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t****v
发帖数: 9235 | 22 傻逼真是傻得可爱
你到底知道我是lesbian还是hetero
你知道我到底是女还是男
猥琐男受了刺激
还心甘情愿把自己排在白男后面
实在是傻逼中的极品 |
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t*******y
发帖数: 21396 | 23 最早是Hetero Junction的德国人Kroemer,然后是半导体激光器的苏联和美国人。他们
拿了，现在中村秀儿也拿了，说实话Holonyak挺亏 |
|
发帖数: 1 | 24 自己查那几个基因吧
看看携带者有多少
We previously reported that a number of patients show a thermal instable
phenotype of compound hetero/homozygous variants of CPT II. To understand
the mechanism of the metabolic disorder resulting from CPT II deficiency,
the present study investigated CPT II variants in patient fibroblasts, [c.
1102 G>A (p.V368I)] (heterozygous), [c.1102 G>A (p.V368I)] (homozygous
), and
[c.1055 T>G (p.F352C)] (heterozygous) [c.1102 G>A (p.V368I)] (
homozygous)
compared with fibroblasts from... 阅读全帖 |
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x****e
发帖数: 1780 | 25 你不是说米国医院撒谎吗，为什么你信这个
: 自己查那几个基因吧
: 看看携带者有多少
: We previously reported that a number of patients show a thermal
instable
: phenotype of compound hetero/homozygous variants of CPT II. To
understand
: the mechanism of the metabolic disorder resulting from CPT II
deficiency,
: the present study investigated CPT II variants in patient fibroblasts,
[c.
: 1102 G |
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m******8
发帖数: 2153 | 26 http://www.pinknews.co.uk/2010/02/18/first-gay-boutique-hotel-to-be-opened-in-new-york/
Developers hoping to capitalise on New York's popularity with gays and
lesbians are to open the first gay boutique hotel in the city.
The Out NYC is billed as the city's first "hetero-friendly" resort, a tongue
-in-cheek reference to premises being described as "gay-friendly".
The $20 million project will see an existing building in Hell's Kitchen
being redeveloped to make a property with 123 guest rooms, a g |
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R**o
发帖数: 311 | 27 those therapies often lead to serious mental disorder/trauma of the
patients. They are enforced to mortify their sexual desires instead of
being converted to hetero. |
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n*******t
发帖数: 7254 | 28 http://www.jeramyt.org/gay/gayhealth.html#prom
There is an extant myth, propagated by the Christian Right, that homosexuals
engage in highly promiscuous behavior. Granted, there are some homosexuals
that do engage in such behavior. But there are heterosexuals that do the sam
e thing. What is important to recognize is the substantial heterosexist bias
that causes people to ignore heterosexual flaws while highlighting homosexu
al flaws.
a) One study which appears to support the idea that homosexua... 阅读全帖 |
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n*******t
发帖数: 7254 | 29 第二个研究有意思，gay里面的多性伴的和无性生活的都比较高，结果平均下来少了。
homosexuals
homosexuals
sam
bias
homosexu
p
Chr
s |
|
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n*******t
发帖数: 7254 | 31 第二个研究结果同时显示滥交的只占同志比例不到20%.
homosexuals
homosexuals
sam
bias
homosexu
p
Chr
s |
|
n*******t
发帖数: 7254 | 32 有些担心一些人会打着争取同志权利的名义来鼓吹滥交正当。你怎么看这个？ |
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m******1
发帖数: 19713 | 33 我本来就觉得无所谓，都是成年人了，自己对自己的行为负责。滥交没错，错的是不安
全的性行为 |
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n*******t
发帖数: 7254 | 34 我也觉得wiener没错呀，可是他硬是被弄下台了。 |
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m******1
发帖数: 19713 | 35 你可真会偷换概念
老百姓的行为和政治人物有可比性吗？
政治人物撒个谎还铺天盖地地报道呢，老百姓能有这待遇？ |
|
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|
|
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n*******t
发帖数: 7254 | 40 但不是他下台的根本原因。更深层次的原因是他的行为不符合主流社会的规范, 会让他
失去一大部分选民的信任。岂止他妻子受了伤害，子女父母朋友都蒙羞了。 |
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m******1
发帖数: 19713 | 41 美国社会对政治人物的行为规范的标准定得比常人高很多，这也是美国的特色，这种事
情如果发生在一些欧洲国家，不会是什么大事。 |
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m******1
发帖数: 19713 | 44 In the midst of a natural disaster (or zombie apocalypse), you don’t want
to be caught without the vital information needed to keep you and your
family safe. In addition to a standard emergency kit (water, food,
flashlights, first aid items), there are several documents that everyone
should have access to at all times. These documents are crucial for same-sex
couples and LGBT parents who might not have the same obvious protections
that their hetero counterparts do in a time of crisis. Experts re... 阅读全帖 |
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h*******g
发帖数: 2201 | 45 In biology, homo or hetero has no bias, my major is bio, so please don't
take it that far, because I don't imply any derogatory, I only know fagot(
not sure about its spelling, but I am sure you know which word I refer to)
implies negative meaning, and I did not use that word, OK? |
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h*******g
发帖数: 2201 | 46 It's a bit contradicting that scientists don't know the cause and conclude
it is not a defect or illness, just like originally scientists thought AIDS
were caused by anal sex? POlitics influence science community, without
question. For example, some famous scientist made a comment about the
variations of IQ in different race, which is a blind truth and cause huge
backfire. It is a shame politics try to blend black and white.
I don't care if you are gay or not, and I do have gay friends. But not ... 阅读全帖 |
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s******y
发帖数: 28562 | 47 Surprisingly, according to results of many researches, that was not true.
The children grew up in a same-sex family have more open attitude to sex and
more of them had at least tried (or admitted to try) same-sex experience,
however, in the end, the percentage of those children end up being
homosexual are not significantly higher than people grew up in a hetero-
sexual family. |
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m**c
发帖数: 7299 | 48 【 以下文字转载自 Gay_Criminolgy 俱乐部 】
发信人: msgc (为了下一代免遭变态残害), 信区: Gay_Criminolgy
标 题: 美华裔男子遭同性恋人刺杀(图)
发信站: BBS 未名空间站 (Thu Apr 3 09:35:06 2014, 美东)
美华裔男子遭同性恋人刺杀(图)
排行榜 收藏 打印 发给朋友 发布者：admin
热度0票 浏览54次 时间：2014年4月02日 11:08
死者(右)与男友去年9月相恋 (图片取自Facebook)
侨报3月31日洛杉矶报道：根据法医办公室消息，3月29日下午于西好莱坞的一栋民
宅中发生的命案，死者为34岁的华裔男子马(Kurtland Ma)，犯罪嫌疑人为死者的室友
同时也是同性男友，36岁的戴维斯(Andre Damiane Davids)。目前嫌疑人已被拘留，保
释金为100万美元。
周六下午，警方接到报案，称位于好莱坞日落大道及圣莫尼卡大道之间的一栋公寓
中有人将室友杀死并企图自杀。探员赫南德兹(Crystal Hernandez)表示，警方赶到案
发现场时，民宅内无人响应，警方破门而入后发... 阅读全帖 |
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l**********1
发帖数: 5204 | 49 to date PD or visiting schloar on next week working dinner (hetero
genfer >90%) every friday afternoon;
to renew/get Cross field GRANT such as MURI, it looks like that
Francis Crick wants to borrow recent Tom Yi
Tang Zhang his primer number theory to fix false RNA-seq out put... |
|
