o**d
发帖数: 3080 | 1 pubmed: Gong Zhang, Zoya Ignatova
1.Polyglutamine expansion alters the dynamics and molecular architecture of
aggregates in dentatorubropallidoluysian atrophy.
Hinz J, Lehnhardt L, Zakrzewski S, Zhang G, Ignatova Z.
J Biol Chem. 2012 Jan 13;287(3):2068-78. Epub 2011 Dec 1.
PMID:
22134925
[PubMed - in process]
2.Length-dependent translation of messenger RNA by ribosomes.
Valleriani A, Zhang G, Nagar A, Ignatova Z, Lipowsky R.
Phys Rev E Stat Nonlin Soft Matter Phys. 2011 Apr;83(4 Pt 1):04... 阅读全帖 |
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l**********1
发帖数: 5204 | 2 if by GFP not luciferase
you can try CAG (cytomegalovirus (CMV) early enhancer element and chicken
beta-actin) promoter
paper link:
//www.ncbi.nlm.nih.gov/pubmed/22378886
Mice strain link:
//jaxmice.jax.org/strain/016532.html
Description
The CAGs-rtTA3 transgene has a CAG (cytomegalovirus (CMV) early enhancer
element and chicken beta-actin) promoter driving widespread expression of
reverse tetracycline-controlled transactivator 3 (rtTA3). High rtTA3
expression is observed in most tissues examin... 阅读全帖 |
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j*****q
发帖数: 82 | 3 sybr green的方法。primer express设计的引物， 没hairpin，没dimer，在载体上扩
增很漂亮，线性化也很好。但是拿到基因组DNA上扩增就很差。 不同的底物浓度的ct值
很接近，而且都偏大，28，29的水平。
这样的现象能是什么问题呢？引物本身的问题吗？
多谢了 |
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y***i
发帖数: 11639 | 4 人的genome有 30亿个base pair,哪里能说避免就避免的。而且每个人的genome又不
同。和一个人的基因不吻合不意味着和别人的基因不吻合。。。。
我一向支持转基因，不过这个RNAi转基因我现在还真不敢支持。。。。
hairpin
的代
不能 |
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C***H
发帖数: 508 | 5 可是做生物的该能看出来，不管有没有primer，oligo1和oligo2都是stable hairpin
without overhang，应该不会有反应才对啊 |
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S**********e
发帖数: 1789 | 6 在开玩笑吧。此人是印度人，Texas Tech的，那几篇牛文章是Harvard, 姓一样，名字
不一样。他就2篇文章。
Sensitivity enhancement and matrix effect evaluation during summation of
multiple transition pairs-case studies of clopidogrel and ramiprilat.
Manjunath Swamy J, Kamath N, Radha Shekar AK, Srinivas NR, Kristjansson F.
Biomed Chromatogr. 2010 May;24(5):528-34. doi: 10.1002/bmc.1322.
Lentiviral delivery of short hairpin RNAs protects CD4 T cells from multiple
clades and primary isolates of HIV.
Lee SK, Dykxhoorn DM, Kumar P, Ranjbar S, So... 阅读全帖 |
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b****n
发帖数: 110 | 7 hsa-mir-155 hairpin 上面貌似有两个functional miRNA，也就命名为-3p 和 -5p。
如果只有一个functional miRNA，和它match 的没有实际功能的就叫做 ＊ |
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s******n
发帖数: 47 | 8 请教版上的大侠，
pol III 转录终止信号一般是TTTTT，现在想用U6 promoter drive shRNA 的表达，而
shRNA的最后两个核苷酸是TT， 比如hairpin的seq是这样的-AAxxxxxxxxxxxxxxxxxxxx-
loop-yyyyyyyyyyyyyyyyyyyyTT-。 现在的问题是转录产物的3‘到底有几个T？ 如果转
录终止信号是TTTTT，那么 转录产物的3‘就可能不是T，因为最后的两个T会被pol III
默认为是转录终止信号的两个TT。
请问大侠，这样的转录产物到底是什么呢？
非常感谢！！ |
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S***a
发帖数: 257 | 9 我刚遇到类似的问题解决了，希望解决方案对你有帮助。
我的质粒表达hairpin shRNA,对细菌有毒性。涂板的时候克隆长出来没有问题，但是摇
菌的时候长的不好，拿到的质粒dna浓度很稀。
我是买来的质粒，所以打电话问了公司客服。他们建议：
1.用 terrific broth (TB)而不是普通LB摇菌；
2.用 carbenicillin，不要用Ampicillin (如果你的细菌是amp抗性的话）；
3.如果还不行，可以尝试room temperature 摇菌两到三天。
我的问题换了TB就解决了，祝你好运！ |
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c*******i
发帖数: 951 | 10 发给最早的文章，见RNAi。根据美国专利局的记录，Graham/Rice/Waterhouse在Fire／
Melo发
文前连hairpin版本都做出来了，但是没用。 |
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x****6
发帖数: 4339 | 11 这个牛大发了，取消了PAM的限制，以及5'hairpin stemloop，而且直接用单链DNA。这
些特质都完败crispr/cas9，效率看数据它们俩差不多。
如果能够得到crispr/cas9在美帝相应的资源投入，对这个自然系统进行工程优化，有
希望把crispr/cas9比下去。后发优势啊！
doudna,church,zhang看到这个工作估计特妈脸都绿了。
不过更有可能的是，作者直接把专利授权/卖给美帝的公司，最后中国纳税人的钱给美
帝的资本家做了嫁衣。 |
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q**x
发帖数: 1636 | 12 【 以下文字转载自 JobHunting 讨论区 】
发信人: quix (uglyDuck), 信区: JobHunting
标 题: Opportunity/Referral - Heat Transfer Engineer
发信站: BBS 未名空间站 (Mon Aug 26 15:39:06 2013, 美东)
I am a consultant in Houston, TX; Oil and Gas companies retain our firm to
find qualified candidates for their open positions. I wanted to see if you
or anyone you know may be interested in a Heat Transfer Engineer role with
one of my clients?
My client is a leading international supplier and licensor of process
technology, catalysts, ... 阅读全帖 |
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t******e
发帖数: 196 | 13 Stabilization of DNA Hairpins by Stilbene Capping of the Terminal Base Pairs
, J. Chin. Chem. Soc., 2006, 53, 1501
Thank you very much for the kind help!!! |
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p**h
发帖数: 99 | 14
that is a tricky stuff.
there are many methods for site-directed mutagenesis. what is in your kit?
anyway,
make sure your oligos are correct (like it can not form a hairpin structure,
ect.)
if possible, you may try other kits. my favorite was a kit from promega
(forget its name), never lost with it. hehe. I hate PCR method (some ones
like it). |
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c*****t
发帖数: 1879 | 15 1: DNA有多少种构型?
DNA structures:
A: more RNA double helix like, 10% of all DNA?
B: the original proposed structure, majority.
Z: GC rich DNAs, left handed, under high salt (2M ?)
and appears in chromosome structure somewhat often.
There is also an over twisted structure, maybe called Y, forgot.
2. 如果supercoil, hairpin, catenated, knob至少要多少个bp才能有这些构型呢?
I don't quite understand your question. The minimum number of bp
for DNA to form a full circl |
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p********y
发帖数: 5141 | 16 小铁，像15mile和30mile这种hairpin怎么练？
我每次都速度降到快成静止了。。。
famed |
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i****x
发帖数: 17565 | 17 找到个网站把这条路列为great driving road
http://motoringconbrio.com/great-driving-roads/
Sonora Pass (California State Route 108 from Sonora to Junction US 395) –
the climb to Sonora pass is unbelieveable…twisties like you wouldn’t
believe, but be careful, there are no warning signs and the road is hard to
read… As soon as you get east of Oakdale, the terrain gets interesting. 108
is full of sweepers and hairpin turns. The road is smooth asphalt and
generally clean. As you climb in elevation, the temperatu... 阅读全帖 |
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i****x
发帖数: 17565 | 18 蒙特卡洛酒店阶梯下的著名hairpin
蒙特卡洛酒店门口的豪车，话说12C挺小挺短的，SLS屁股真难看，怪不得被人骂，有点
像acura那种中间一条楞的设计。
再贴下隔壁贴过的欧洲汽车见闻：
最近在欧洲大陆流窜，注意了一下汽车。欧洲街上过十辆车才有一辆带屁股的sedan，
可能要100辆才有一辆suv。大多数车都是subcompact，小部分compact。不夸张地说，
mini在这里算中等大小的车，只有smart才能让人觉得是小车。当然，偶尔来几辆法拉
利还是有的。意大利山顶上听到远处的引擎呼啸声，远远看去，山路中间一个红点，望
远镜才看出是458，好不拉风。法国大半都是法国车，雪铁龙和破肉（忘了怎么翻译了
），德国vw，意大利好车、大车多点，见到不少alpha romeo 147。奔驰见到的都是A和
B，C见过一两次，见到的E系全是出租车，到是S系见过很多，都在酒店门口接人。bmw
1 3 5 7各见过几辆，也很少。欧洲的豪车都是给美国造的，鉴定完毕。 |
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L*****g
发帖数: 311 | 19 我看你说的highway 9的那个高手开得很快啊，50/60过弯，甚至70吧。还没有响胎，所
以如果放到赛道上，应该算是连续的sweeper了，不应该对应到hairpin上。赛道的连续
弯也主要靠维持momentum啊，跟山路类似。不过有一个区别就是犯错空间的大小吧，赛
道上犯错还能保命，山路上犯错就惨了。如果让auto pilot开，应该没太大区别，让人
开山路上心理压力就大多了，所以一定得慢点儿开才行。 |
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w*******y
发帖数: 60932 | 20 Enter code "SHIPSFREE" at checkout.
Can alternatively be bought through Amazon with Discovery as the seller if
it saves taxes-(I cant tell, I get taxed on everything but food out of
Amazon) but the Discovery direct site guarantees delivery by 12/24.
Link:
http://store.discovery.com/detail.php?p=86155
Create colorful drawings as you drive this remote-controlled ladybug across
art surfaces.
Fun for boys and girls ranging in age
Makes hairpin turns and spins
Encourages hand-eye coordination
Sized f... 阅读全帖 |
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c*******r
发帖数: 13580 | 21 TR: Kangaroo Temple rock climb
We drove to Blue Lake Trail head Friday night and slept in the car. Saturday
morning at 6, we met at the hairpin (it's about half mile past Washington
pass if you drive east on North Cascades highway, you can't miss it). The
approach begins from here. There is no well maintained trail. You have to
find your way in the boulders and bushes. There are patches of snow but
nothing bigger than half of a basketball court. No ice ax needed as of mid
July 2008.
We encounter |
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