S**********g
发帖数: 109 | 1 现在在研究glial cell(神经胶质细胞)的一些功能，急需能阻断或抑制glial cell的阻
断剂，但是网上找了一些要么就是不能完全阻断要么就是毒性太高，哪位高手知道这方
面信息的吗？谢谢了 |
|
|
S**********g
发帖数: 109 | 3 就是阻断glia cell的活动，神经系统有两种细胞，一种是神经细胞也就是神经元，另
外一种就是glial cell也就是神经胶质细胞，想找到一种抑制剂，只抑制神经胶质细胞
但不抑制神经细胞 |
|
f**d
发帖数: 768 | 4 这是一本计算神经科学的优秀著作，全文拷贝这里（图和公式缺），有兴趣的同学可以
阅读
如需要，我可以分享PDF文件（－－仅供个人学习，无商业用途）
From Computer to Brain
William W. Lytton
From Computer to Brain
Foundations of Computational Neuroscience
Springer
William W. Lytton, M.D.
Associate Professor, State University of New York, Downstato, Brooklyn, NY
Visiting Associate Professor, University of Wisconsin, Madison
Visiting Associate Professor, Polytechnic University, Brooklyn, NY
Staff Neurologist., Kings County Hospital, Brooklyn, NY
In From Computer to Brain: ... 阅读全帖 |
|
e***o
发帖数: 344 | 5 glial cells 对大多数neuroscientist来说，直接就被忽略不管了。随着技术手段的提
高，比如在glial cells弄chr2等等， glial cells的作用被渐渐的挖掘开来了。。。
想和大家讨论一下，glial cells到底是确实功能上底neuron一等呢？还是不好做，比
如不好patch，杂乱等等。。。。
这里求一片文章，谢谢，好像很有意思的哦。
http://onlinelibrary.wiley.com/doi/10.1002/glia.21096/pdf
Roles of glial cells in neural circuit formation: Insights from research in
insects
l******[email protected] |
|
e***o
发帖数: 344 | 6 首先感谢文献帮助!!!
是否可以这么说，大多数 glial cell 主要功能是supporting ，保护，修复neurons。
但是glial cell太多了，分类不清。就如interneuron一样，没有好的marker， cre-
line就无从下手一样。
但是越来越多的功能正在被挖掘，譬如分泌axon guidance 信号, 回路布线，
tripartite synapse，neurotransmitter releasing, clearance。。。。。
也有些文章开始提Glial cell plasticity，虽然有炒作概念之嫌，但也并非孔穴来风
。最近也读过几篇Glial cell和记忆的文章，好像就是通过营养等通路来影响的。
去年science一片很有意思的paper，分享一下，
呼吸-----co2 浓度----ph 改变--- astrocytes ca2+ dependent ATP release---
activate chemoreceptor neuron.
应该说neuron还是执行者，但是glial cell的作用应该是被underestim... 阅读全帖 |
|
h*******o
发帖数: 4884 | 7 For primary neurons, we use neurobasal media +B 27. It is selective towards
neurons and glia usually cannot survive for long term in NBM+B27. With that
said, there is no pure neuronal culture. Anyway, cultured neurons do exhibit
some glial properties, such as glucose transporter expression, metabolic
activity, etc.
For embryonic glial culture, we use DMEM/F12 (1:1) +10% FBS. Because glial
cells are mitotic whereas neurons are post-mitotic, neurons will be easily
outnumbered by proliferative glia... 阅读全帖 |
|
m*******e
发帖数: 1598 | 8 BY TINA AMIRTHA8 MINUTE READ
This story contains interviews with Christian Kästner, assistant
professor at Carnegie Mellon University; Amy Hirotaka, state policy and
advocacy manager at Code.org; and Janet Siegmund, researcher at the
University of Passau.
In order to expose more students to computer science, 17 states have passed
legislation to create basic math and science requirements in the curriculum,
rather than count them as electives. But lawmakers in Texas have made
computer program... 阅读全帖 |
|
|
f*******3
发帖数: 122 | 9 RE: Applicant’s Name
To Whom It May Concern:
It gives me great pleasure to write this letter in support of Dr. XXX’s
research contributions to the field of ____________________.
I am Chair of Molecular Biology at the School of Biological Sciences at the
University of ____________________________. I have spent _______ years in
academic research, publishing approximately _________scientific publications
in peer-reviewed, internationally distributed journals, which have been
cited ________ tim... 阅读全帖 |
|
p*****c
发帖数: 20445 | 10 Marc R. Freeman, Ph.D.
HHMI Early Career Scientist
University of Massachusetts Medical School
Neurons hog the attention, but our brains couldn’t operate without the
unassuming neural cells called glia. Marc Freeman is working to bring these
overlooked cells—which represent about 60 percent of brain cells—their
share of credit.
Freeman has been dissecting the role of glia in brain development, function,
and healing after injury, when glia hurry to the damaged area and devour
cellular debris to al... 阅读全帖 |
|
d****h
发帖数: 46 | 11 For rat neuron
GABRA1: (Neuron. 1989 Dec;3(6):745-53.A novel alpha subunit in rat brain
GABAA receptors.Khrestchatisky M, MacLennan AJ, Chiang MY, Xu WT, Jackson MB
, Brecha N, Sternini C, Olsen RW, Tobin AJ.)
GABBR2:similar to GABRA1, highly neuron-specific
MAP2:(A Harada, J Teng, Y Takei, K Oguchi, N Hirokawa
MAP2 is required for dendrite elongation, PKA anchoring in dendrites, and
proper PKA signal transduction J Cell Biol, 158 (2002), pp. 541–549)
Tuj1: (J Neurobiol. 1995 May;27(1):26-43.Div... 阅读全帖 |
|
l*w
发帖数: 567 | 12 没错，glial cell 主要是supporting neurons. 只是neuron是神经系统的主角，所以
以前大家把研究重点放在了神经元上了。但是研究的继续深入必然就要涉及所有参与者
，而且要治疗SCI之类的疾病，没胶质细胞也不行。当然，也不能低估在发育或损伤修
复中glial cell 和神经元的相互作用。这些事件中的机理，都有理论和应用的价值。 |
|
p****p
发帖数: 3360 | 13 Very interesting input.
What kind of neuro-protection function do you think is the most important
role for glial cells? Let's say, dopamine hyperactivity has been suggested
for some Parkinson's. Is there anyway to manipulate/intervene glial cells
to prevent or delay PD from happening?
microglia
outcomes |
|
m*********k
发帖数: 10521 | 14 成功奖励 20 伪币的用户:
flyingmd, aagent, waiting140, glial, MKZ, shiyuemudan, milkswan, kalel, chrr
, penguinfly, MarsLynn, baytennis, movinghut, insvf, ddtus, reknaz,
bigapples, MakingWaves, pathdream, lvhemi, dongogogo776, mountin2010, loveAP
, SinoGator, diandian23, autoking, lookacar, nothim, mySpringCa,
TheIslander2, sunnyjoy, Trim, tuantuan007, ycnan, foxer, saintknight,
tinyrice, huaimao1124, shiyuemudan, cxzrka, haipan, zxnk, PharmD, kenchendz,
kubusek, qiushuiyiren
奖励版面:(astrology)20伪币成功
奖励版面:(... 阅读全帖 |
|
r********n
发帖数: 7441 | 15 发信人: conti (conti), 信区: TsinghuaCent
标 题: zz pk饶毅进入第二轮的张旭的背景
发信站: 水木社区 (Sun Aug 28 15:38:09 2011), 站内
如果神经方向就只能上一个候选人，那么张旭入选还真是一点也不出格
发信人: WeiLiao (WeiLiao), 信区: Biology
标 题: 张旭的publications
发信站: BBS 未名空间站 (Sun Aug 28 01:39:20 2011, 美东)
去查了一下，张旭总共发表了90多篇英文文章（不算中文）。回国之后作为联系作者也
有快20篇了。其中有cell, neuron, Journal of neuroscience, PNAS 好多篇。
而且他
1995年就回国了，那个时候国内的环境有多差不用说了吧。这也快20年了。比饶益
2007
年才全职回去多了12年。比起培养的中国学生也多多了，算国内贡献肯定不会小。如果
他一直留在国外，成就未必就比饶差。谁把饶益的文章列一列。国内多少篇，总共多少
篇，培养了多少中国学生？
1. Li KC, Wang F, ... 阅读全帖 |
|
|
发帖数: 1 | 17 We are seeking a highly motivated post-doctoral research fellow to
investigate the molecular mechanisms of neurodegeneration in neural diseases
including stroke using both in vitro model of primary cultured neurons and
glial cells and in vivo mouse model. The position is available immediately.
Qualified candidates must hold a Ph.D. degree or equivalent degree (
preferably a recently received degree) in neuroscience, physiology, cell
biology, bioengineering or a related field. Excellent oral and ... 阅读全帖 |
|
发帖数: 1 | 18 A postdoctoral position is available immediately in the laboratory of
Professor Shinghua Ding at Dalton Cardiovascular Research Center, the
University of Missouri-Columbia. The candidate will investigate the
molecular mechanisms of neurodegeneration in neural diseases including
stroke using both in vitro model of primary cultured neurons and glial cells
and in vivo mouse model. Qualified candidates must hold a Ph.D. degree or
equivalent degree (preferably a recently received degree) in neuroscie... 阅读全帖 |
|
A*****a
发帖数: 52743 | 19 可以这么说。干细胞本应该分化成astrocyte或者glial cell的，但没有完成便出现癌
变。 |
|
s******e
发帖数: 493 | 20 Bless!
What is a rhabdoid tumor?
A rhabdoid tumor is a rare and highly malignant tumor of childhood, first
described in 1978. These tumors were initially considered an aggressive
variant of Wilms' tumor of the kidney, however, with newer diagnostic
techniques, these tumors are believed to represent a distinct entity. Since
that time, there have been fewer than 50 cases reported, although it is
likely that some cases previously identified as medulloblastoma or as
primitive neuroectodermal tumo... 阅读全帖 |
|
r********3
发帖数: 306 | 21 http://www.newsrx.com/newsletters/Pain-and-Central-Nervous-Syst
Amphetamines
Research Data from Mayo Clinic Update Understanding of Amphetamines
Published in Pain and Central Nervous System Week, July 2nd, 2012
2012 JUL 2 (NewsRx) -- By a News Reporter-Staff News Editor at Pain &
Central Nervous System Week -- Data detailed on Amphetamines have been
presented. According to news reporting originating in Rochester, Minnesota,
by NewsRx journalists, researchers stated "Glutamate signaling plays an
... 阅读全帖 |
|
a*********0
发帖数: 213 | 22 Very small paper, no impact factor
journal biochemistry insight
Title Extracellular Matrix and Integrins in Embryonic Stem Cell
Differentiation
Abstract : Embryonic stem cells (ESC) are pluripotent cells with great
therapeutic potentials. The in vitro differentiation of ESC was designed by
recapitulating embryogenesis. Significant progress has been made to improve
the in vitro differentiation protocols by toning soluble maintenance factors
. However, more robust methods for lineage specifi... 阅读全帖 |
|
G****a
发帖数: 10208 | 23 【 以下文字转载自 Biology 讨论区 】
发信人: glial (胶质), 信区: Biology
标 题: 十年的轨迹
发信站: BBS 未名空间站 (Tue Jun 12 13:06:47 2012, 美东)
2000年在西部的一个小有名气州立大学里有六、七个国内来的博士们在做生物博士后。
他们的本科有来自复旦、清华、南开，也有来自湘雅、同济这样的医学院，而博士均是
毕业于中科院在北京或上海的几个生物相关的研究所。因为科研上做的大方向相似，又
都是中科院出来的，所以大家无论在上班或业余都有很多的交往。在最初的几年每人都
有在本领域top 1,2的杂志上发表的第一作者文章，有的还有CNS。
十年之后，大家都有了自己的生活轨道，却也是各不相同
1. 04年回国做了中科院某所的PI，继续在科研上努力进取
2. 在做完第一个薄厚，辗转换了几个实验室，完成了从千老到staff scientist的蜕变
，最近拿到几个faculty的位置，终于遂了做老板的心愿
3. 一直在同一个实验室，目前是lab manager，然而并未在科研上继续，转而研究房地
产，在房价低谷时滚雪球般的投资了七... 阅读全帖 |
|
s***a
发帖数: 384 | 24 neuroscience... 确切说是glial cells... |
|
a******g
发帖数: 129 | 25 astrocytes and glial cell are non-parenchymal cells in brain. They are the
other side of biology compared to various famous parenchymal cell types such
as neurons,beta cell, hepatocytes and so on. These non-parenchymal cells
provide unique niche to maintain the normal function of specific tissues and
organs. |
|
a******g
发帖数: 129 | 26 actually the developmental biology of glial cells is very elusive.
No one exactly knows how they differentiate and where they develop from.
Maybe you can be the one:)) |
|
S*****s
发帖数: 287 | 27 时间越长 neuron 越不容易培养，到最后长出来的都是 glial cell。还是尽量取年幼
的老鼠做效果比较好。 |
|
n***w
发帖数: 2405 | 28 Angiotensin and bradykinin: targets for the treatment of vascular and neuro-
glial pathology in diabetic retinopathy.
Please send to n***[email protected]
Thank you!!! |
|
W*****o
发帖数: 1780 | 29 去查了一下，张旭总共发表了90多篇英文文章（不算中文）。回国之后作为联系作者也
有快20篇了。其中有cell, neuron, Journal of neuroscience, PNAS 好多篇。而且他
1995年就回国了，那个时候国内的环境有多差不用说了吧。这也快20年了。比饶益2007
年才全职回去多了12年。比起培养的中国学生也多多了，算国内贡献肯定不会小。如果
他一直留在国外，成就未必就比饶差。谁把饶益的文章列一列。国内多少篇，总共多少
篇，培养了多少中国学生？
1. Li KC, Wang F, Zhong YQ, Lu YJ, Wang Q, Zhang FX, Xiao HS, Bao L, Zhang X
. (2011) Reduction of follistatin-like 1 in primary afferent neurons
contributes to neuropathic pain hypersensitivity. Cell Res, 21: 697-699.
2. Li KC, Zhang FX, Li CL, Wang F, Yu MY, Z... 阅读全帖 |
|
m********a
发帖数: 239 | 30 Read it here: http://the-scientist.com/2011/08/31/puncturing-the-myth/
Puncturing the Myth
Purinergic signaling, not mystical energy, may explain how acupuncture works.
By Geoffrey Burnstock | August 31, 2011
4 Comments
According to traditional Chinese medical theory, acupuncture points are
situated on meridians along which qi, the vital energy, flows. However, I
have proposed a less mysterious neurophysiological mechanism to explain the
beneficial effects of this 2,000-year-old practice (Medica... 阅读全帖 |
|
c********n
发帖数: 67 | 31 Glial Response and Delayed Myelin Clearance in Area of Wallerian
Degeneration after Spinal Cord Hemisection in the Monkey
Thanks a lot for your kindly help
h***[email protected] |
|
w*e
发帖数: 740 | 32 果蝇的larva时期中枢神经系统神经干细胞的特异的分裂方式，很适合研究非对称分裂
，不过现在小鼠和人的脑细胞（比如outer radial glial cells）理论上也是很好的模
型。 |
|
c********n
发帖数: 67 | 33 Ultrastructure of rabbit retinal nerve fibre layer--neuro-glial
relationships, myelination, and nerve fibre spectrum
Circadian rhythmicity in neutrophil chemotaxis.
Thanks very much for you kindly help
h***[email protected] |
|
c********n
发帖数: 67 | 34 1, Ultrastructure of rabbit retinal nerve fibre layer--neuro-glial
relationships, myelination, and nerve fibre spectrum
2, Circadian rhythmicity in neutrophil chemotaxis.
3, Concentration of astrocytic filaments at the retinal optic nerve junction
is coincident with the absence of intra-retinal myelination: comparative
and developmental evidence
h***[email protected]
Thanks very much for you kindly help |
|
n*******l
发帖数: 190 | 35 MACS已经很简单了，但是glial progenitor cells也表达glast |
|
N******n
发帖数: 3003 | 36 先分化。glial cell is major cell type in the nerve system. |
|
N******n
发帖数: 3003 | 37 先分化。glial cell is major cell type in the nerve system. |
|
c**********r
发帖数: 891 | 38 Wiki: Crick published "The Astonishing Hypothesis" in 1994，positing that "a
person's mental activities are entirely due to the behavior of nerve cells,
glial cells, and the atoms, ions, and molecules that make them up and
influence them."
这么明显的东西还需要写一本书？
明显是在骗纺锭，或者trolling宗教人士为乐。 |
|
i*****e
发帖数: 633 | 39 我不做DRG。我同事的DRG养得不错。开始时候遇到的问题是glial太多，在DRG neuron
下形成了个"毯子"，然后peel off连同neuron一起飘走了。后来加了阿糖胞苷以后就好
了。
不过说到电生理，倒有新的问题请教。我们看到的现象是，只有10%不到的
microelectrode可以有AP信号。然后大家的结论是，neuron必须正好长在电极上才行。
我一方面记得有这个说法，说是cell/electrode overlap的面积达，接触紧，那么信号
就好。另一方面，我觉得AP是个电压信号，微电极在溶液里捕获电压信号，貌似不用贴
得很紧，好像有个文章里说过，隔10个um都能感受到的。也不知道哪个说法是对的。 |
|
z****u
发帖数: 1007 | 40 不知道你试过Wnt1-Cre cross with reporter没。因为PNS全部来自于neural crest
cell. 所以Wnt1-Cre 标记PNS非常不错。我正好拍过肠子上的照片。这里贴几张供你参
考。这是Wnt1-Cre;R26-ZsGreen flox reporter ， mesentery上面伴行动脉静脉的神
经fiber 以及glial cells. |
|
|
b*********e
发帖数: 870 | 42 还是没看懂。你要inhibit glia 的啥活动？分裂？移动？还是别的啥？ |
|
b*********e
发帖数: 870 | 43 metabolic inhibitor，这种毒性不会小的，而且paper也说了，是acute inhibition，
如果想长期global inhibit cell activity，怎么样都会是“毒性”不小的。 |
|
|
h*******o
发帖数: 4884 | 45 直接换到Neurobasal media +B27 去养，这样基本glia就长不了了 |
|
j******5
发帖数: 183 | 46 大家好，
攒review申请绿卡，真诚希望大家能推荐审稿的机会。愿意提供简历，非常感谢！
本人背景：Ph.D., major in Neuroscience and developmental biology. Graduated
in 2010 and current position is associate research scientist.
Keywords: developmental biology, neuroscience, glial cell, oligodendrocyte,
myelination, white matter injury, dopamine receptor, G protein-coupled
receptor
Email: y********[email protected]
Thanks,
June |
|
W*****s
发帖数: 684 | 47 Carboxymethylchitosan/Poly(amidoamine) Dendrimer Nanoparticles in Central
Nervous Systems-Regenerative Medicine: Effects on Neuron/Glial Cell
Viability and Internalization Efficiency Salgado, A.J., Oliveira, J.M.,
Pirraco, R.P., Pereira, V.H., Fraga, J.S., Marques, A.P., Neves, N.M., (...)
, Sousa, N. 2010 Macromolecular Bioscience 10 (10), pp. 1130-1140
Thanks a lot! |
|
d****y
发帖数: 2180 | 48 ☆─────────────────────────────────────☆
jiyimobuqu (jiyimobuqu) 于 (Wed Sep 26 19:55:33 2012, 美东) 提到:
毕业年限：近20年，
USMLE step 1: 83%
USMLE step 2 78%
Step 2 CS:第二次过。
没有Externship,只有一次正式Observership还没有推荐信，靠在医院做义工候着脸皮
要了两份推荐信。。。
可是，我刚刚收到一封面试邀请信！使劲揉揉眼，没错，是面试邀请信！
我都能行，你们更行！大家加油！
☆─────────────────────────────────────☆
warmlion (warmlion) 于 (Wed Sep 26 20:02:45 2012, 美东) 提到:
恭喜！你一定有自己的闪光点。好好把握每一个面试，祝你成功MATCH！
☆─────────────────────────────────────☆
jiyimobuqu (jiyimobuqu) 于 (Wed Sep 26 2... 阅读全帖 |
|
d****y
发帖数: 2180 | 49 ☆─────────────────────────────────────☆
skyscorpio (天之蝎子) 于 (Thu Nov 1 23:56:44 2012, 美东) 提到:
这篇文章主要是想说说如何判断自己在申请时的定位的问题。完全来自我个人肤浅而有限
的人生经历，或者是道听途说到的故事，辅以丰富的想象以及我过人的总结能力 LOL
所以只是一家之言，欢迎大家发表不同看法。
先说一个最大的道理：connection beats everything else. 下面的讨论是在没有
connection的情况下。
不同科的申请，看重的方面显然不同，先说说内科的申请。内科申请总的形势是match
进去比较容易，但是想进好点的比如说大学的program比较难，虽然我也搞不清楚大学
的program好在哪里。
我觉得内科申请第一看重毕业年限。所以，如果你打算申请内科，尽量别拖太久，毕业
十年是一个大限，过了十年，你申请全国所有的内科programs,你的面试很难超过10个
（有关系的除外，超大牛的除外，惊为天人的除外，以下同）。换一句话说，如果你毕
业很久了，对自己也不... 阅读全帖 |
|
G*********y
发帖数: 100 | 50 glioblastoma是astrocytoma的一种。
astrocytoma就是从astrocyte来源的肿瘤，GFAP阳性。
成人的Glioblastoma multiforme (grade IV astrocytoma)
儿童的Pilocytic (low-grade) astrocytoma
是FA上需要掌握的两种astrocytoma，成人的恶性程度很高。
astrocyte就是一种Glial cells，所以你看GBM的名字就可以理解了。 |
|
