m******5
发帖数: 1383 | 1 小弟现在正在做Chip
所用抗体IP effeciency尚可
目前打算用此抗体做Chip,不过问题来了，sample crosslink, lysis， 以及dilute后
用Beads-antibody pulldown，之后用protein loading buffer煮30分钟后上SDS-page.
跑SDS-page后目的带附近什么也没有。 同一块胶的阳性对照也正常
input目的带也没有跑出来
请问有同学遇到过这种问题么？
补充说明一下，Chip所用材料是invivo knock in了一个tag的老鼠的组织。 |
|
c****h
发帖数: 4968 | 2 看了一个报道，军事管制造成的延误只有7%,绝大部分是航空公司处理不当和空中交通
指挥不effecient。不过宁肯晚一点，空域利用的effeciency低一些，安全到达就好。 |
|
h******m
发帖数: 425 | 3 没有说房价高是否合理，只是说明房地产的重要性
没有这些年的建设，绝对不会有那么多人想要回国享受国内的好处。
如果不是因为美国建设的太落后，也绝对不会有那么多人在这边呆着不爽老想往国内跑的
房子之所以比iphone贵，是因为它不像手机一样可以批量生产。了解房地产行业就知道
这是一个需要投入太多effort却产出不那么effecient的行业。
总之一句话，不要享受了好处却take for grant. |
|
s**t
发帖数: 17016 | 4 你就是典型的根本不了解人家究竟要干么，就根据自己的想象批上了。
杨安泽并不反对AI发展，而是支持AI发展。每一次工业革新，都会导致大量工人失业，
从而导致暴力、混乱和抵制。杨安泽是要让这个过程变得更smooth，更soft，也更
effecient
。 |
|
u******b
发帖数: 483 | 5 so, 楼主想说什么呢？ 抱怨美国医疗费用贵？ 这个是全世界人都知道的，也是为什么
每次大选的总统候选人的施政计划里都肯定要谈的。 如果真要跟中国比的话，美国和
中国的医疗体系正好是一个硬币的两个面：
1. 美国的医疗体系是最不体现“市场经济”原则的成本奇高；而中国的医疗体系是过
渡开放缺少监管造成的另外一个极端。
2. 中国重效率（Effeciency），轻effectiveness。 (就如500个人拥挤在医院排队：
好处是很多人都能让见到医生；但这个经历太可怕了。） |
|
C****n
发帖数: 2324 | 6 We should say NO to any measure raising TAX.
Folks, when you run out of money, what do you do?
First thing first, you need to eliminate your wasteful spending! Make
yourself effecient!
Then if it's still not enough, borrow from your friends/families.
That's whay we need to do: California has enough money! We just wasted too
much. Ask for more money is ridulous! I will say NO Even raise money for the
KIDs! Let's get take a look of what money we can save to give to the KIDs
first! |
|
C****n
发帖数: 2324 | 7 First, you got it all wrong.
We do not want to hear people who has money, we want to hear people who
contribute to this country, and help others, people who pay TAX.
We do not want to hear people who does not contribute, but keep demanding
more money from others.
Why do you have a problem with people who has money? I love them. They "
exploited" me, but I love them. I beg them to "exploit" me. They give me job
, they pay more tax helping the country.
You are right we need to go forward. But you ... 阅读全帖 |
|
C****n
发帖数: 2324 | 8 1. TAX/Money is the most effecient way to idnetify your contribution to this
country. I don't see any other easy ways to do this.
Besides, when people contribute, we reward them with money. Give them an
"honor" like what we do in China, is fucking insulting their intelligence!
Money is what I pay for your service, Period. So if you give money to the
country, you are contributing to the country. Don't tell me those great
scientist, artist, sportman, Police, Marine, who contribute a lot too.... 阅读全帖 |
|
j****3
发帖数: 682 | 9 直接忽略了。如版上所说，model老，价格没有优势，fuel effeciency 也没有什么优
势。all in all, cl除了丰田造，其它好像毫无起色。 |
|
j****3
发帖数: 682 | 10 直接忽略了。如版上所说，model老，价格没有优势，fuel effeciency 也没有什么优
势。all in all, 除了丰田造，其它好像毫无起色。 |
|
f********e
发帖数: 166 | 11 We have a N X N matrix whose rows and columns are in sorted order. How
effeciently can we find
the median of those N^2 keys ? |
|
s******e
发帖数: 108 | 12 We have a N X N matrix whose rows and columns are in sorted order. It is
called as Young's Tableau matrix. How effeciently can we find the median
of those N^2 keys ? |
|
R*******G
发帖数: 21 | 13 今天通知fail了。。
给大家分享个题目看看， 献丑了。
如果有更好的做法大家集思广益。
可能还是不够强大吧。
上来问phd项目，然后一直搞不懂我phd研究的意义。一直保持微笑拍马屁应对。
问完坏笑一个，问tcp reno和tcp vegas，我答对了。
然后系统设计题：单个机器上面很多table，然后thread pool容量有限，然后外部一个
很大的queue有很多update要处理。如何effecient的处理请求。size (queue) >> size
（table）>> size (thread poll)
我说两个方案：
一个把对table的updates hash 到 size of（thread poll），这样thread只处理自己
一部分table，不会有contention。但是hash的时候要根据table 的traffic hash，否
则一些thread会starve很长时间。
还有一个就是每个table自己有个local queue，当多个threads访问一个table的时候，
它们等待，超时以后把要写的updates放在那个table的queue上，... 阅读全帖 |
|
E**********e
发帖数: 1736 | 14 今天比较高兴， 网上搜了个比较简单的xml parser，比较efficient。 本来在real
xml file上测试了，比较满意。但是换到了experian 的xml files。 竟然有bug。 花
了一天的时间debug。最终搞定了。 总共就几个简单的functions， 用recursion。竟
然很好的解决的experina的问题。 说到这，不得不赞自己一下。 学习能力还是很厉害
的吗。IT组的一个资深软件工程师用C#编的parser，用了most brute force 的
algorithm，答应出来竟然要10多页纸。
另外，也要赞一下python。 发现用python处理 xml file，很有效。 xml转成
dictionary data structure， 然后到json，到dataframe，到mongoDB. 一下子全通了
。 |
|
|
|
|
E**********e
发帖数: 1736 | 18 pyhton 是有开源的。
不过，要是是么都拿开源的， 自己怎么会提高？ 是不是。 自己通过这个项目，很好
的明白了xml的数据结构，同时加深理解了python dictionary的数据结构。再加上
mongodb，json 数据结构。好像hive也就类似的数据结构。
而且pytyon dataframe 太赞了。 即使有的key missing， 但是最终dataframe 可以让
他们现行。很容易debug。
而且我这个比开源的要快。这是关键。 我现在能够熟练的操作xml， mongodb， sql。
这些是金融行业比较流行的数据结构。 我向data scientist 迈进了一小步。 |
|
p***r
发帖数: 4702 | 19 支持xpath 之类吗？这些玩意要一做一套，或者符合iso 不然没有什么卵用。
你的xml parser 支持namespace 吗 |
|
|
E**********e
发帖数: 1736 | 21 还不懂xpath。
现在是从xml转化到python dict。 namespace有相应的函数处理掉。
这个parser是用来处理工作的数据。目前没有必要考虑iso是么。也许处理别的vendor
的xml还会有bug。不过即使有debug，再改进也行。 核心的东西就是recursion。 而且
也有附属的数据转化功能， xml 相应的text 转化成相应的数据类型。
总的来说， 处理experian的信用报告的xml 也就足够了。 |
|
|
p**r
发帖数: 5853 | 23 你被骗了，一般真的认真学习的，都没时间来发帖，
吃饭拉屎都在想进化。 |
|
E**********e
发帖数: 1736 | 24 mitbbs上垃圾挺多的，充诉各个版面，见不的有人夸自己好。像您这位就是。
我吃饱了撑的，没事发个假贴，引来您这尊大神
确实该高兴。马工的是干不了。但作为data scientist，coding能力胜任的。这也是自
己正在提高的方向。工作中有时就应该自己找活干。我这个parser一搞定，就可以绕开
IT。不是说IT干的不好。不同组之间有时工作安排不同，必须等。 |
|
m********8
发帖数: 938 | 25 microwave basic, transmission line, smith chart, strip line , microstrip
line.
impedance matching, LNA, NF, Sensitivity, IP3, 1db compression, PA, loadpull
, Effeciency, PAE,
GSM,CDMA, WCDMA, LTE system and test
basic programming, C |
|
w********0
发帖数: 1995 | 26 相比Lowes Samsung那个deal哪个好呢，LG那个没有wool cycle，不知道这个是不是很
重要，但是看了很多评论，似乎samsung主要的评价是安静，但是马达有坏的评价，LG
有评价说洗得很干净。还有samsung high effeciency是不是说必须用high effecienty
的洗衣粉呢？多谢！ |
|
h****h
发帖数: 1168 | 27 just my personal feel:
for moving company, chinese are great. they are effecient and not lazy.
for installing hardwood floor, no. already got enough bad example from
mitbbs.
. |
|
j*********n
发帖数: 90 | 28 high effeciency 是 >92% AFUE. 现在的furnace 至少80% AFUE. high efficiency
furnace 要接PVC 管子把废气通到外面。如果你旧的furnace大小适当的话，用
90000BTU times 旧的furnace的efficiency 再除以新的furnace的efficiency 就是新
furnace的 BTU.80% efficiency 的furnace 可以仍然用chemney 排气 |
|
a***n
发帖数: 1616 | 29 Looks nice. How much is this set? $2K?
There're a lot of redunant or nice-to-have tools that however cannot cover
many necesary auto repair projects.
But anyway, I have a lot of custom special tools collected purely based on
specific project needs, and now I'm happy every time when I got more stuff
to make my tool set more complete:)
And adding a tool box or cart is nice as well, but has to be cost effecient. |
|
r*********s
发帖数: 2157 | 30 I have to store some tools, so I bought a tooxbox. Then I bought more tools
to fill in the box, then I need another toolbox, then I need to fill in it..
..
effecient. |
|
w*****m
发帖数: 20421 | 31 氟利昂啊，新空调不用氟利昂了，怀疑你家空调不EFFECIENT，看看出风口的温度是多少 |
|
x****u
发帖数: 12955 | 32 Last year we installed a new 160000 btu 95% effecient furnace . It cost
around $1400. |
|
l******c
发帖数: 2555 | 33 ETF for foreign currency trade, safe but not effecient.. |
|
|
s****n
发帖数: 17 | 35 Data from last quarter's ER.
Pro:
revenue increasing a lot
large amount of repeat orders (Plus New orders)
competitive advantage (less space and higher effeciency)
exploring market in China
opened office in Beijing recently
(朋友小道消息说，国内销量喜人，供不应求)
very little long term debt
ER soon
5 baozi to PM you the pick.
If anything mentioned above is not true, you are welcomed to criticize me in
this Forum. |
|
c****i
发帖数: 2635 | 36 对。
先查一下员工手册看看是否有言语滥用和种族歧视这方面的规定。有的话是最好了。还
要加上一些可以预见的影响。
hr...working on sth.
lz: Hi xxx, how are you doing?
hr: ...
lz: r u busy at the moment? or i can drop by -- when might be a better time?
hr如果说有时间，继续，如果没时间，就会给你个时间，就可以说谢谢，那到时候见。
假设继续—— 全程要保持微笑气质！！
lz: just wondered if there's any changes to the regulation number _x_ bullet
point _y_ recently?
hr: which one exactly?
lz: the regulation about making offensive comments and being racist.(如果楼
主同事说话的时候不但讲到china这个字眼，还用上chinese，一定要扯上racist)
hr大概会翻条例什... 阅读全帖 |
|
h*i
发帖数: 27 | 37 V good suggestions. In my case, my major examiner and another minor examiner
stayed out of the confusing knots (or pretend to, I don't know), and I have to
deal with the other minor examiner, who was former chair of grad students.
During preparation for the exam, we were working much more effeciently than me
with my major examiner. Now really can't tell what he said was truth, and what
was to confuse me, and what else is to give me hints for alternatives. :<
Try to be optimistic even though the |
|
g*********r
发帖数: 2847 | 38
one day i told my colleagues i cooked at home, and they said, " you are an
actuary. it is not effecient for you to cook." From then on, i've never
cooked. |
|
I*****y
发帖数: 6402 | 39 ☆─────────────────────────────────────☆
baboben (baboben) 于 (Tue Nov 29 20:58:32 2011, 美东) 提到:
http://www.mapleleaffarms.com/194
☆─────────────────────────────────────☆
molle (SimpleMolle) 于 (Tue Nov 29 22:47:01 2011, 美东) 提到:
牛，啥coupon都有！
☆─────────────────────────────────────☆
maywhite (梅逐雨中黄) 于 (Wed Nov 30 11:21:43 2011, 美东) 提到:
爱吃~~~
☆─────────────────────────────────────☆
TVMAN (灰灰) 于 (Wed Nov 30 12:06:01 2011, 美东) 提到:
怎么觉得最近附近中国人吃鸭子的多了，成天有人做咸水鸭。是不是流行这菜谱
☆───────────────... 阅读全帖 |
|
l*****r
发帖数: 15615 | 40 你们房子新，空调很effecient，我们家是被后面的泳池拖惨了。 |
|
o**********e
发帖数: 18403 | 41 thanks for baozi. The water and air doesn't show significant radiation
increase at the current level. Also my geiger counter is not water proof.
at <100 becquerel/liter, the reading won't be significant unless i carefully
manage to gather all the particles across the 1 liter container.
I measure thyroid because its concentrate iodine (including radioactive)
with high effeciency (within a day). so it's easier to measure and early to
tell any significant difference. |
|
f*u
发帖数: 5576 | 42 That house has better heating system than average.
Heat pump is using electricity to move energry around, it's good for heating
and cooling.
Heat pump is usually a lot more effecient than both electricity or forced
air,
but the efficiency of heating is degrated when tempature dropped to certain
threshold, its efficiency could be worse than using electricity for heating
directly when outside is really cold.
That's why there is still backup heat source such as electricity or gas
furnace.
In Seattl... 阅读全帖 |
|
s*******n
发帖数: 12995 | 43 ☆─────────────────────────────────────☆
yogibear (yogibear) 于 (Tue Mar 20 17:58:15 2012, 美东) 提到:
该房子的信息如下：
heat source： electricity
heat system： heat pump
之前看到的房子的对应信息一般是：
heat source： gas
heat system： forced air
请问该房子的加热系统有什么不好么？
☆─────────────────────────────────────☆
sonofagun (止戈为武) 于 (Tue Mar 20 18:05:47 2012, 美东) 提到:
heat pump就像空调，只不过是到冬天可以反过来。一般heat pump是从空气中抽取热量
，但气温低于冰点，就不好了。更好一点的heat pump是从土壤里抽取热量，土壤相当
恒温。
heat pump初装贵，修理贵，但平常花费少。
force air 就是烧个炉子吹热风，初装，修理便宜但平常花费要大。
☆────────... 阅读全帖 |
|
H**********e
发帖数: 1069 | 44 音乐楼到我这儿一定会歪成八卦楼的。
离婚贬值说的是普通人。名女人越嫁越好的多的是，不管是自己赚的名声财富还是前夫
家给的名声财富。古今中外啊，数都数不过来了，杨贵妃，宋丹丹，朱玲玲，jackie
kennedy, diana。。。还是climb social ladder重要，到时候自然就一览众山了。
婚姻市场highly effecient |
|
s*******h
发帖数: 3219 | 45 【 以下文字转载自 JobHunting 讨论区 】
发信人: RichyDING (RickyDING), 信区: JobHunting
标 题: amazon 系统设计题 和 遇到阿三的经历
发信站: BBS 未名空间站 (Thu Apr 16 06:55:07 2015, 美东)
今天通知fail了。。
给大家分享个题目看看， 献丑了。
如果有更好的做法大家集思广益。
可能还是不够强大吧。
上来问phd项目，然后一直搞不懂我phd研究的意义。一直保持微笑拍马屁应对。
问完坏笑一个，问tcp reno和tcp vegas，我答对了。
然后系统设计题：单个机器上面很多table，然后thread pool容量有限，然后外部一个
很大的queue有很多update要处理。如何effecient的处理请求。size (queue) >> size
（table）>> size (thread poll)
我说两个方案：
一个把对table的updates hash 到 size of（thread poll），这样thread只处理自己
一部分table，不会有contention。但... 阅读全帖 |
|
h********n
发帖数: 5148 | 46 HOME WARRANTY的原则是能修就修,我有个客户的房子空调都已经很坏了,找了外面好几
家公司QUOTE,都说需要换了.但是HOME OWNER WARRANTY的CONTRACTOR来了,换了个零件
修好了,不管功率是不是还那么EFFECIANT, 反正修好了, 就不会给换了.
记住一点,HOME OWNER WARRANTY只是对FAILURE负责的.所以看你换空调的原因是什么.
如果现在还没坏,快坏了,就赶紧买一个.如果已经坏了,但是你能忍受2, 3 weeks 没有
空调.那你可以现在买,然后2, 3 周后CLAIM. 但是就算空调REPLACE了,也不是说,你不
用付一分钱的.
看来,我又应该写一个选HOME OWNER WARRANTY的咨讯了. |
|
|
n******e
发帖数: 140 | 48 After the game my face was all burning so i went outside to smoke, and the
cold air and 2 feet of snow did calm me down quite a bit.
I was thinking, ppl might say it finally showed Ben was a rookie, but i feel
that's not a totally fair assessment. I think ultimately the Steelers WR should
shoulder at least some blame for the lose. Half way thru the game it's obvious
either side could run the ball effecient, as underdog pointed out the Pats
constantly put 8 or 9 defenders in the box, they stopped |
|
n******e
发帖数: 140 | 49 first, i find it hard to play run and even harder to play defense
with mouse&keyboard. If u want to enjoy this game to the fullness
i suggest u get a gamepad or joystick.
Ok, to run effeciently, u first need to understand the play u called.
That means know where to run before snapping the ball.
1. Try to follow the fullback who might create a hole for u in line of
scrimmage when u call a run up in the middle, if u didn't see a hole
immediately, try use "junk left/right", to shift to outside, the |
|
n******e
发帖数: 140 | 50 I sat at the first row at an endzone.
ND's offense is pretty much a carbon copy of Pats', only
it worked much more effeciently against the Wolverines.
>70% short passes to stretch the defense, and a surprising
running call here and there. The end result is <30% running
calls but more rushing yards (97 yards) than passing (94 yards)
in the first half. The score is 14-3 until the last 2 mins of
the game.
They absolutely killed us. |
|
