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g*********1
发帖数: 37 | 3 附件里面有用NIH droplet分析后图，更明显些 |
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s**s
发帖数: 122 | 4 我觉得这个根本就没有什么问题。只是因为中间那条带signal弱，所以看起来象是不同
的背景。
把帖子删了吧。
背靠背两篇cell的第一作者都是中国人，很好的事情。这些工作不做3－5年做不出来。 |
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g*********1
发帖数: 37 | 5 我也作过很多western blot,条带信号弱的话，背景应该一致，这条带的背景明显和两
边的不一致，懂行的过来看看 |
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g*********1
发帖数: 37 | 6 你辛辛苦苦做实验，人家copy paste 想要的结果，你很高兴吗？这样做是research
misconduct.需要向学校和nih反映 |
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s**s
发帖数: 122 | 7 羡慕嫉妒恨。莫名其妙。
首先，这个东西是不是copy paste凭你那个分析根本不能说明什么问题。有时候如果条
带比周围的条带信号弱，背景看起来会弱些。而有时条带信号强会使得背景看起来也强
些。
其次，这个工作，看这个文章的工作量，人家肯定是辛辛苦苦做了很多年，而觉不是
copy paste想要的结果。我们应该为这样的同胞高兴。
再次，楼主在第一个帖子里问别人这个图影响不影响结论。既然这个图影不影响结论楼
主都没有搞清楚，想必没有好好读这个文章。我就搞不明白楼主为什么对一个不去好好
读明白的文章的supplemental figures这么感兴趣。 |
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s**s
发帖数: 122 | 9 呵呵。为牛人当了一回辩护人。
牛人不需要辩护人。 |
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s*****j
发帖数: 6435 | 10 你贴几张你做的胶上来, 看看能不能调出什么"copy and paste"? |
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s*****j
发帖数: 6435 | 11 "我们对科学社区的责任", "科学社区"--真是做学问的人呀. |
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n****e
发帖数: 327 | 12 This is outrageous!
On the original scan, from left to right, Lanes 7 and 9 were overloaded,
resulting in higher level signals for all three proteins. The Vinculin
signal was very strong and reached saturation, so that the difference in
loading wasn’t obvious. Yet, lanes 7 and 9 were overloaded as indicated by
the non-specific bands between 76kDa and 120 kDa.
However, in the published figure, the P-p53 and p21 blots were flipped
horizontally, but the Vinculin blot wasn’t flipped. In this way the... 阅读全帖 |
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h****s
发帖数: 40 | 13 真牛人。完全同意这个分析。
唉。这个行业要自律呀。
科学社区的人们呀，聪明人加上不自律，比copy paste更过分呀。把人耍了还数钱呀。
by |
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s**s
发帖数: 122 | 14 这个分析很有道理。
希望作者不是有意的。
我为牛人的辩护是基于图片不是”copy paste“。
by |
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y******8
发帖数: 1764 | 16 依照经验，total protein没有over-load，lane之间的差异在20%以内。
150kd附近的带，量差异在100%以内。
p53, p21 positive的lanes比，量差异在5倍以上。
by |
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M******t
发帖数: 555 | 17 没看懂.意思即使不over-load,也有显著差异?
20%成100%到底有没有over-load? |
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n****e
发帖数: 327 | 18 Anyone who understands the basic principle of ECL knows its linearity is
quite limited. It turns to exaggerate differences between weaker signals but
minimize and underestimate the differences among strong saturated bands.
When the immunosignals are weak, a 2-fold change may appear to be 10-fold on
films. It is hard to know what the real result was given the information
here is limited, but it is rather puzzling that the author made such an
effort on flipping the images. Regardless of the tru... 阅读全帖 |
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y******8
发帖数: 1764 | 19 经验就是经验，你做过一千个WB就知道了。
我没看出作者在这张film上刻意manipulate Expo Time的地方。
but
on |
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n****e
发帖数: 327 | 21 Flipping and mis-presenting original images is clearly a research misconduct
that can’t be tolerated. It is not about how many westerns you have done.
What being questioned is the author’s integrity! |
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y******8
发帖数: 1764 | 22 那你去告啊。
别人最多加个erratum. 什么都没影响。我觉得作者很坦荡，integrity不比各位差。
misconduct
. |
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p****n
发帖数: 9263 | 24 靠，还真是这么回事
这位”胸怀坦荡“的牛人敢贴出原图来，真是小觑了天下英雄啊
by |
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l**********1
发帖数: 5204 | 26 想将来坐美国牢狱里边的 尽管copy and paste
then published at NCS except N sub |
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s*********t
发帖数: 600 | 27 怕坐牢，不敢copy and paste就老老实实滴做自己。不要JJYY，眼红别人copy and paste。没必要 |
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l**********1
发帖数: 5204 | 28 是吗
咋看 象 第五纵队的一份子呢
我不怕虎一样的对手 就怕猪一样的队友 更忌蛇一般的潜伏者
是啥名句吧 啊 |
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s*********t
发帖数: 600 | 29 哎，人家辛辛苦苦好几年发一篇Cell，正高兴呢，当头一盆冷水。
别回应别理睬，做自己的。越回应越来劲，人们都喜欢看热闹看笑话。
方舟子之流就是这么利用人们的心理的 |
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l**********1
发帖数: 5204 | 30 this kind of Cell contents including SOM contents correction if met "11thJigen"
might be 2nd Kato S possible:
>Jigen says he began looking into Kato's publications after a colleague told
him about a correction to a 2009 Nature paper that appeared in October 2011
. Several images "were inadvertently duplicated or erroneously created
during figure assembly," the correction notice reads. Jigen and his
colleague then examined 24 papers from the Kato group and spotted 60 those
allegedly problematic... 阅读全帖 |
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s**s
发帖数: 122 | 31 我为牛人的辩护是基于图片不是copy paste。
唉。我只知道开始，不知道结局。 |
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M******t
发帖数: 555 | 32 我开始怀疑这个贴子是作者内斗了,sigh....
发这么牛文的一作来贴图不能认真点么? |
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r***u
发帖数: 50 | 33 在我看来只有p53的原图被贴了出来，vinculin和p21都不是原图。 |
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c****d
发帖数: 681 | 34 同感。原图那个vinculin和published差别有点大啊。而且原图中lane4到9基本上是一
直往下走，published的图，patter明显不一样。 |
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M******t
发帖数: 555 | 35 图是原图吧？不过可能是扫描的时候不小心翻过来扫了。 |
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z********i
发帖数: 610 | 36 看图上标注的marker，应该是vinculin翻转了一下。如果重新跑一下western，可能没
有什么问题。 |
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l**********1
发帖数: 5204 | 39 薄希踢断其老爸薄一几根肋骨后 还在当三番王还妄想进京当常委的那教育出来的
学术政治上 高级黑的概率上比其它宗教教育出来的高点 正常啦 |
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m**********3
发帖数: 706 | 40 看了这个帖子，我对做生物一点兴趣都没有了，决定华丽丽的转行了。感觉现在的生物
就是一个给别人挑毛病的专业。不懂得欣赏别人，造成这个领域的方舟子这样的人越来
越多。同时，发文章的为了不让别人挑毛病，造假也越来越多，最后成为一个前人骗后
人，后人骗后后人的烂摊子。我现在做的领域，可以说30-50%的前人的结果重复不出来
。不光是我重复不出来，公开报道的互相扯皮打架的就有无数。造成几大学霸相互排挤
，投文章时老板写的要回避的名单都是一大串。这种经历，您们有木有，有木有。 |
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l**********1
发帖数: 5204 | 41 转进合成人工系统生物学/计算人工生物学/计算人工生物数学吧
不用造假 本来就是假的
反正可以 指鹿为马 隔年又可以指马为驯鹿的学科啊
总指挥是
//www.hhmi.org/news/lim_bio.html
//limlab.ucsf.edu/papers.html |
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r*****u
发帖数: 56 | 43 A postdoctoral position is immediately available in the newly established
Cheng Lab, Department of Human Nutrition, Food and Exercise, Virginia Tech.
Motivated professionals, who are interested in research on type 2 diabetes,
obesity and cancer [1-7], are welcome to apply. Strong background/expertise
in biochemistry, molecular biology and/or cell biology is required. Previous
experience in signal transduction pathway analysis [1-3,5-7], mitochondrial
function [2,6,7], or redox (oxidation/reducti... 阅读全帖 |
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p*****m
发帖数: 7030 | 44 看了名单才发现居然好多以为是院士的人其实不是。。Rick Young, John Carlson,
Roberto Malinow, nancy bonini, ron depinho, greg hannon,alex rudensky...
牛！这里面好几个人我都去听过讲座，都挺impressed, 想不到这么快就都纷纷院士了。
唯一的例外就是小薇，她一共去过我以前那个学校三次，每次我都是正好有其他实验安排
而没有去听。所以其实我从来没有见过她。
另外，Chang, Yuan貌似年轻时候是个美女呢。
Macromolecular Imaging, Baylor College of Medicine, Houston
Sciences Endowed Professor of Biology, department of biology, Duke
University, Durham, N.C.
department of biology, Stanford University, Stanford, Calif.
professor of chemistry and che... 阅读全帖 |
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d*****r
发帖数: 2583 | 45 Zhang Feng以前的老板:
Deisseroth, Karl; associate professor of bioengineering and psychiatry,
Stanford University, Stanford, Calif.
竟然是Associate Professor...就当选院士了，史无前例的Rising Star...
http://www.nasonline.org/news-and-multimedia/news/2012_05_01_NA
News from the National Academy of Sciences
Date: May 1, 2012
FOR IMMEDIATE RELEASE
National Academy of Sciences Members and Foreign Associates Elected
The National Academy of Sciences today announced the election of 84 new
members and 21 foreign associates ... 阅读全帖 |
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O******e
发帖数: 4845 | 46 象这种层次的，50％肯定是不止的。我以前也稍微查过一些，比方R. DePinho和
S. Elledge，都是七八成的人都独立了。 |
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n*********b
发帖数: 140 | 47 If DePinho is forced to resign, the biggest loser is MD Anderson.
NIH Director has identified translational medicine as a new frontier, in its
effort to bring NIH funded research to clinic. Two problems are topping
the list of road blockers. One is the definition of "conflict of interest";
the other is the current system of "peer review".
The public, especially the media, needs to think about whether we can avoid
all forms of "conflict of interest", and at what cost.
Scientists need to speak ... 阅读全帖 |
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l******n
发帖数: 298 | 49 Ronald A. DePinho
王晓东啥的跟他比还是一般吧。
牛人太多了。 |
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j*b
发帖数: 341 | 50 王晓东是诺奖级别的。Ronald A. DePinho is very productive. |
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