f**r
发帖数: 5 | 1 最近可能需要做个简单的proliferation assay,就是转染个基因然后几天过后数细胞数
量。据说转染率在80%左右。首先其实想问的就是做这个试验需要注意什么么？
其次，如果结果阴性的话，还有必要作cell death assay么？我记得曾经读过一篇文章
，说一个东西对proliferation没影响，但对cell death有影响（忘了他们是用flow还
是什么了）。板上有前辈能解释一下cell death assay一般怎么做的么？谢谢。 |
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k********0
发帖数: 126 | 2 【 以下文字转载自 Seattle 讨论区 】
发信人: kennyvt100 (kenny|vt100), 信区: Seattle
标 题: NYU Professor Jumps to Death From 16th Floor Apt
发信站: BBS 未名空间站 (Fri Jan 15 09:59:28 2010, 美东)
NYU Professor Jumps to Death From 16th Floor Apt
Posted on 14 January 2010
A NYU professor committed suicide by jumping from his 16th floor Greenwich
Village apartment in Manhattan, New York.
NYU professor Sam Roweis jumped to his death from his Greenwich Village
apartment Tuesday, and friends and colleagues still cannot understan |
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w*********s
发帖数: 2136 | 3 【 以下文字转载自 Parenting 讨论区 】
发信人: whiteclouds (/ 参考消息 /), 信区: Parenting
标 题: Vaccination Link To H3N2 Death Cluster In Japan Hospital?
发信站: BBS 未名空间站 (Thu Nov 11 12:47:57 2010, 美东)
http://www.recombinomics.com/News/11071001/H3N2_Akita_Vaccine.html
Vaccination Link To H3N2 Death Cluster In Japan Hospital?
Recombinomics Commentary 13:22
November 7, 2010
25 patients and 8 staffs are suffering from influenza now
29 Oct., vaccinated against the flu
31 Oct., a man died(eighties)
2 Nov., 2 men died(six... 阅读全帖 |
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p********y
发帖数: 5141 | 4 In November 2011, the Washington Post printed an article entitled "Deaths in
triathlons may not be so mysterious; panic attacks may be to blame." The
article noted that there were 9 deaths that occurred during triathlons in
2011, and 8 of them occurred during the swim. The article also noted that
deaths occurred in triathlon at a higher rate per participant than
comparable mass participation endurance sports. |
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w*******y
发帖数: 60932 | 5 "This is the F-15C "Death Rider", an aircraft featuring a special skin. A
Skin Design Contest was previously featured on the internet. The Death Rider
won with the most number of votes out of the 821 total entries! This
fantastic design is available free of charge. *This item contains the same
data featured in Compatibility Pack 1. After downloading this item, you do
not have to download Compatibility Pack 1."
Link:
http://marketplace.xbox.com/en-US/Product/F-15C-Death-Rider/93f
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s*****t
发帖数: 1994 | 7 A Dark Sky Over Death Valley
Credit: Dan Duriscoe, U.S. National Park Service
Explanation: This eerie glow over Death Valley is in danger. Scrolling right
will show a spectacular view from one of the darkest places left in the
continental USA: Death Valley, California. The above 360-degree full-sky
panorama is a composite of 30 images taken two years ago in Racetrack Playa.
The image has been digitally processed and increasingly stretched at high
altitudes to make it rectangular. In the foregrou |
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w********h
发帖数: 12367 | 8 唔，这个问题...
还是活命重要吧。
选择：
(1) Live with dignity;
(2) Live without dignity;
(3) Live with humiliation;
(4) Death with dignity;
(5) Death without dignity;
(6) Death with humiliation.
如果我选，
首先（1），
（1）做不成的年代，
偶就做逍遥派，
什么dignity or humiliation,
身外之物，
活命要紧。 |
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U*E
发帖数: 3620 | 9 http://io9.com/mechanic-accused-of-inventing-a-death-ray-for-is
 http://img.gawkerassets.com/img/18rbluohnpgmxjpg/k-bigpic.jpg" onload="adjustimg(this)">
A mechanic who worked at General Electric in New York spent the past
several years perfecting a deadly, truck-mounted radiation weapon. According
to the FBI, he tried to sell it to some Jews in his area, whom he figured
could hand it over to Israel. Failing that, he tried to sell it to some
friends in the KKK.
Glendon Scott Crawford is... 阅读全帖 |
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y***l
发帖数: 6963 | 10 这是咋回事儿？
Posted on Wed May 12 2010 13:32:16 GMT-0400 (Eastern Daylight Time) by
PapaBear3625
The FINANCIAL -- According to RIA Novosti, the death toll from riots on May
2 in China's Muslim Xinjiang Uygur Autonomous Region has risen to 140.
Riots began in the regional capital of Urumqi on May 2 when a group of
protesters demanded an investigation into the deaths of two ethnic Uighurs
killed in a fight with Han Chinese workers at a toy factory in late June.
Xinhua said protesters from the Uighur Mu |
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u****n
发帖数: 7521 | 11 几十个国家统计，倒数第三。不过，印度在中国后面。
It rates 40 mostly rich countries by how well they care for the dying.
Britain tops the table. For all the health care system's faults, British
doctors tend to be honest about prognoses, the mortally ill get plentiful
pain killers and a well-established hospice movement cares for people near
death. Countries such as Denmark and Finland rank lower because they
concentrate more on preventing death than on helping people die without
suffering pain, discomfort and distress. |
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u****n
发帖数: 7521 | 12 DEATH AND DEBT ...
How insane
does it have to get?
We let Bush
"stay the course"
of America's nightmare.
Our trust dutifully applied
to an administration that lied.
Was it all too surreal
for Americans to deal?
We who enabled his power.
We continued as cowards.
Lazy, crazy or numb.
We were bottom-line dumb.
Lemmings that cliff-fell to sea
behind Bush's rash policies.
DEATH AND DEBT ...
How insane
does it have to get?
We let Obama
"stay the course"
of America's nightmare.
Our trust dutifully appl |
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W**********n
发帖数: 187 | 13 The Cook County medical examiner’s office has determined a 19th person died
of heat-related causes following last week’s heat wave.
Dorothy Hawkins, 69, of the South Chicago neighborhood, died of heart
disease, with heat stress as a secondary cause, in an accident, the medical
examiner’s office found this afternoon.
Officers were called to the 7400 block of South Yates Boulevard at 5:24 p.m.
Monday after a family member called police to say the woman who lived there
had not spoken with family si... 阅读全帖 |
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W**********n
发帖数: 187 | 14 Los Angeles (CNN) -- California authorities are investigating the death of a
worker in an oven at a Los Angeles-area tuna processing plant.
The victim, Jose Melena, was cooked to death in a steam oven at the Bumble
Bee Foods plant in Santa Fe Springs on Thursday, said Erika Monterroza, a
spokeswoman for the California Division of Occupational Safety and Health.
Melena was a six-year veteran of the plant, which was shut down until Monday
, the company said.
"The entire Bumble Bee Foods family is ... 阅读全帖 |
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G***G
发帖数: 16778 | 15 http://www.huffingtonpost.ca/2013/02/21/elisa-lam-autopsy-missi
An autopsy has not yet been able to determine the cause of death for Elisa
Lam, a Vancouver woman whose body was found in a hotel rooftop water tank in
Los Angeles.
Lam, 21, was last seen in the lobby of the Cecil Hotel on Jan. 31. A staff
member, who was investigating guest complaints of low water pressure,
discovered her body in a water tank on Tuesday morning.
An autopsy was completed on Thursday but the case of death has been "
... 阅读全帖 |
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D**S
发帖数: 24887 | 16 http://io9.com/mechanic-accused-of-inventing-a-death-ray-for-is
A mechanic who worked at General Electric in New York spent the past
several years perfecting a deadly, truck-mounted radiation weapon. According
to the FBI, he tried to sell it to some Jews in his area, whom he figured
could hand it over to Israel. Failing that, he tried to sell it to some
friends in the KKK.
Glendon Scott Crawford is an industrial mechanic in Galway, NY, and he loves
to play with electronics. He is also allegedly ... 阅读全帖 |
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g**1
发帖数: 10330 | 17 New 'Death to America' Songs Unveiled in Iran
TEHRAN, Iran November 2, 2013 (AP)
Associated Press
Hard-liners in Iran have unveiled two new "Death to America" songs at the
former U.S. Embassy in Tehran, hoping to keep anger high ahead of nuclear
talks with Western powers.
They performed the songs Saturday ahead of a planned massive protest Monday
to mark the anniversary of the U.S. Embassy takeover in 1979.
The anniversary this year is drawing greater attention due to hard-line
opposition to mod... 阅读全帖 |
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g**1
发帖数: 10330 | 18 New 'Death to America' Songs Unveiled in Iran
TEHRAN, Iran November 2, 2013 (AP)
Associated Press
Hard-liners in Iran have unveiled two new "Death to America" songs at the
former U.S. Embassy in Tehran, hoping to keep anger high ahead of nuclear
talks with Western powers.
They performed the songs Saturday ahead of a planned massive protest Monday
to mark the anniversary of the U.S. Embassy takeover in 1979.
The anniversary this year is drawing greater attention due to hard-line
opposition to mod... 阅读全帖 |
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h****n
发帖数: 2250 | 19 【 以下文字转载自 Parenting 讨论区 】
发信人: littletalks (monster), 信区: Parenting
标 题: Speaking of bullying, Steven Liu jumped to his death
发信站: BBS 未名空间站 (Tue Nov 5 21:24:56 2013, 美东)
High school stunned after talented track star, 17, 'who could be hard on
himself' jumps to his death from pedestrian bridge
Steven Liu jumped from a bridge near his Chula Vista school on Friday
Friends said he suffered low self esteem and could be hard on himself, even
though he was a talented runner and artist
Others said he... 阅读全帖 |
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T*****o
发帖数: 53 | 20 【 以下文字转载自 SanFrancisco 讨论区 】
发信人: TDTurbo (GoneWithWind), 信区: SanFrancisco
标 题: Death Valley camping
发信站: BBS 未名空间站 (Tue Jan 14 21:29:52 2014, 美东)
Death Valley camping site 是先到先得，打算四月底全家去试一晚，有谁去过，安全
吗 ？ 有啥要注意的 ？ |
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n**********5
发帖数: 1707 | 21 不通英语的发小犹他等了4个小时等待救援车。救援车用了不到10分钟换了备胎。摇摇
晃晃又上路。今天打电话给我告诉我被困在Death Valley了。
我已经警告过他几次。一定要在中午前离开Death Valley。下午有雷暴。结果命中注定
。他们游完Badwater美洲大陆最低点走错了路。没有原路返回而是继续向前走。哪方向
就是N年前死人有数的地方。结果如约碰到下午的雷暴。
还好他们命不该绝。估计本身煞气重还有真丝楠木珠链护体。上一次发小在川藏路遇到
泥石流醒来整辆车里面都是石头他都没死。看着漫山遍野的洪水冲下来沧海横流凭着RV
冲过去。挣扎着回到Furnace Creek做面条。与世隔绝看着水位不断地上涨。
公园管理员告诉他们方圆两百英里的道路都封了。要等大水过去再说。我深深地祝福他
们还好你们有RV。虽然空调不work。 |
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G*********e
发帖数: 2091 | 22 【 以下文字转载自 USANews 讨论区 】
发信人: dealfinder (Deal+Findddddder), 信区: USANews
标 题: 请各位好好看过death by china
发信站: BBS 未名空间站 (Fri Dec 23 15:27:30 2016, 美东)
Death By China: How America Lost Its Manufacturing Base (Official Version)
https://www.youtube.com/watch?v=mMlmjXtnIXI
这个家伙还真是中国通，虽然是有点贬低中国，但是真的非常一针见血。中国和共产党
政府是两回事
Trump选人真是非常牛 |
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c*********l
发帖数: 3438 | 23 【 以下文字转载自 USANews 讨论区 】
发信人: dealfinder (Deal+Findddddder), 信区: USANews
标 题: 请各位好好看过death by china
发信站: BBS 未名空间站 (Fri Dec 23 15:27:30 2016, 美东)
Death By China: How America Lost Its Manufacturing Base (Official Version)
https://www.youtube.com/watch?v=mMlmjXtnIXI
这个家伙还真是中国通，虽然是有点贬低中国，但是真的非常一针见血。中国和共产党
政府是两回事
Trump选人真是非常牛 |
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u***r
发帖数: 4825 | 26 https://www.marketwatch.com/story/kobe-bryants-death-messages-of-shock-and-
sadness-pour-in-2020-01-26?mod=hp_minor_pos19
Kobe Bryant, one of the all-time NBA greats — a legend even before he
finally hung it up in 2016 — was reportedly killed in a helicopter crash on
Sunday.
He was 41 years old.
TMZ reported that Bryant was one of at least four passengers in the private
helicopter flying over Calabasas, Calif., when a fire broke out and caused
the chopper to fall from the sky, killing everyone o... 阅读全帖 |
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h*********n
发帖数: 11319 | 27 BREAKING: A 7th person with coronavirus has died in the Seattle area. This
time, the infection wasn’t identified until well after the person's death.
The death was 6 days ago at Harborview Medical Center.
往好里说这是美国政府透明空开的体现
往差里说这tmd测一个中一个，之前干啥去了？抢救的医护测没测？有多少人没测？ |
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n*********n
发帖数: 1534 | 28 【 以下文字转载自 ChinaNews 讨论区 】
发信人: bballfun (xixi), 信区: ChinaNews
标 题: information source of "1.2 million death of Tibetan"
发信站: BBS 未名空间站 (Wed Apr 16 02:16:49 2008)
Sorry for not being able to type these in Engilish.
Since ZD proudly annouces "1.2 million death of Tibetan" everywhere, I did a
bit research on where the number comes from. Following is waht I found:
1. http://www.tibetgov.net/
This website seems to one of the Official ZD organization. It has many
publications, which we should study |
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J*V
发帖数: 3150 | 29 New 'Death to America' Songs Unveiled in Iran
TEHRAN, Iran November 2, 2013 (AP)
Associated Press
Hard-liners in Iran have unveiled two new "Death to America" songs at the
former U.S. Embassy in Tehran, hoping to keep anger high ahead of nuclear
talks with Western powers.
They performed the songs Saturday ahead of a planned massive protest Monday
to mark the anniversary of the U.S. Embassy takeover in 1979.
The anniversary this year is drawing greater attention due to hard-line
opposition to mod... 阅读全帖 |
|
U*E
发帖数: 3620 | 30 Los Angeles (CNN) -- California authorities are investigating the death of a
worker in an oven at a Los Angeles-area tuna processing plant.
The victim, Jose Melena, was cooked to death in a steam oven at the Bumble
Bee Foods plant in Santa Fe Springs on Thursday, said Erika Monterroza, a
spokeswoman for the California Division of Occupational Safety and Health.
Melena was a six-year veteran of the plant, which was shut down until Monday
, the company said.
"The entire Bumble Bee Foods family is ... 阅读全帖 |
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发帖数: 1 | 31 And this is the law of nature, everyone can not escape. Li Zhufo not
deceived because some people from death; the disciples not ever-young
because of the inevitable death of Lee and Zhufo. According to check, Li
Zhufo's father Li Dan died in 1996. At that time, already "complete" Buddha,
known to "save the world" but Li Hongzhi father died of it; as "Buddha Pro"
Li Zhufo Pro brother, HealthDay News Group Vice President Li Jiguang, died
in May 2012, known as the Li Zhufo equal to anything, they w... 阅读全帖 |
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l****z
发帖数: 29846 | 32 Freedom in Egypt, whatever it may eventually look like, isn’t going to
include the ability to speak negatively about Islam…
CAIRO (AP) — An Egyptian court convicted in absentia Wednesday seven
Egyptian Coptic Christians and a Florida-based American pastor, sentencing
them to death on charges linked to an anti-Islam film that had sparked riots
in parts of the Muslim world.
The case was seen as largely symbolic because the defendants, most of
whom live in the United States, are all outside... 阅读全帖 |
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l****z
发帖数: 29846 | 33 by Rick aka Mr. Brutally Honest
I stumbled across this story on Facebook this morning:
After a two and a half year legal battle, 15 tons of cheese made and
aged near Mountain View was MorningLandDiaryhauled to a dump. To fans of
natural foods, it is monumental waste and over-regulation. To Missouri's
Milk Board, it's merely protecting public health.
"I see the destruction of what my wife and I and family have worked to
build," said Joseph Dixon, owner of Morningland Dairy.
Dixon an... 阅读全帖 |
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l****z
发帖数: 29846 | 34 Nine-Year-Old Boy Shot to Death in Chicago; Obama, Holder, Sharpton
Unavailable for Comment
by Jammie via Jammie Wearing Fools
No cheap political points to score here, so move along, everybody.
A 9-year-old boy was fatally shot Wednesday afternoon in the backyard of
an apartment building in the Greater Grand Crossing neighborhood on the
South Side, authorities said.
Police responding to a call of shots fired in the 1200 block of East
71st Street about 4:05 p.m. found the boy shot multipl... 阅读全帖 |
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发帖数: 1 | 36 Two illegal aliens will spend decades in prison after tying up a 90-year-old
farmer in Minnesota and beating him to death last April.
Reinol Vergara and Edson Benitez pled guilty to second-degree murder and
assisting to second degree murder.
“They left him helpless on his living room floor as they ransacked his home
, as he was bleeding to death, they cut the phone line and left him for dead
,” Carver County Attorney Mark Metz said at the sentencing, ABC 5
Eyewitness News reports.
The alien kill... 阅读全帖 |
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g********2
发帖数: 6571 | 37 口号是black life matters，但事实却是black death matters.
当一个black还是life的时候，上至奥巴马，下到左逼媒体，都不关心他过什么样的生
活，犯多少罪。black只是一只票蛆，用得着的时候就拉拢一下，用不着的时候就把他
们keep在落后愚昧野蛮的状态。
只有当一个black拒捕或袭警被打死之后，他的death才matters了，媒体像打了鸡血一
样兴奋，奥巴马也长篇大论，要求所有美国人为此产生负罪感。 |
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v*********u
发帖数: 10464 | 40 【 以下文字转载自 Military 讨论区 】
发信人: vincentsjtu (国际会议-联合国采购), 信区: Military
标 题: 普京又来找打了“death of liberalism”
发信站: BBS 未名空间站 (Fri Jun 28 07:33:49 2019, 美东)
普京又来找打了“death of liberalism”
G20听到很棒的一句话，Liberalism is far from ideal but it’s the least of
evil. “自由民主”让每个人都有发声的机会，非常棒！
但没有“度”的自由太可怕，21世纪需要一个更高效的制度！
“独立思考能力缺失”的人容易被“别有用心”的坏人带偏！
李光耀年轻时的新加坡也有非常evil的黑历史，但现在这个自由的“度”，新加坡掌控
的还不错，虽然新加坡模式缺点很多，差不多和美式民主一样多缺点，当然台湾式民主
缺点更是多到可怕
收藏 |
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h******o
发帖数: 807 | 41 【 以下文字转载自 Travel 讨论区 】
发信人: heroyoyo (heroyoyo), 信区: Travel
标 题: 在Death Valley开车，可以开冷气吗？
发信站: BBS 未名空间站 (Fri May 14 19:27:43 2010, 美东)
在Death Valley开车，可以开冷气吗？
车子会不会过热啊？
5月底去，谢谢！ |
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d*******s
发帖数: 15155 | 42 My 2 cents
The so-called “death wobble” is relatively common among vehicles that use
a solid front axle, and the Wrangler is the only light truck currently on
sale that falls into this category. The design is prized for its extreme off
-road and heavy duty hauling capability. The wobble is a harmonic resonance
resulting from a loose or worn part in the steering or suspension, such as a
tie rod end, and usually set off by hitting a bump at a certain speed. It
often goes away after the vehicle sto... 阅读全帖 |
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i****a
发帖数: 36252 | 44 前两天才有人说轻轻了一下前车，油漆都没掉，但前车司机要去看医生
这保险陪付，涉及到人生伤害就没谱了。
还是death rate 可信。而且death rate 跟 crash test 结果吻合
bumper
[发表自未名空间手机版 - m.mitbbs.com] |
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B*Z
发帖数: 7062 | 45 GM 手上的人命又要增加了。而且开除了几个讼棍
She said G.M.'s tally of 13 deaths and 54 accidents was based on “the infor
mation we have right now,” but said that figure could grow in the weeks ahe
ad.
Fifteen G.M. employees, including at least three senior corporate lawyers,
were dismissed for their role in the problem, and five others were
disciplined.
http://finance.yahoo.com/news/g-m-chief-says-death-150750764.ht |
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n**r
发帖数: 67 | 46 life insurance covers accidental death, but not the otherwise around. ADD
doesn't cover normal cause of death.
If the insured have both and die due to accident, then both benefits are
paid. |
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r****9
发帖数: 4961 | 47 ADD covers both death and disability.
Life only covers death. |
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s********k
发帖数: 6180 | 48 【 以下文字转载自 CS 讨论区 】
发信人: silverhawk (silverhawk), 信区: CS
标 题: The death of Intel Labs and what it means for industrial research?
发信站: BBS 未名空间站 (Fri Apr 8 21:34:20 2011, 美东)
http://matt-welsh.blogspot.com/2011/04/death-of-intel-labs-and-
Intel觉得花钱不值得？看不到前景？大批researcher失业？下面的回复也很有意思
matt就是前段时间辞去harvard正教授去google那位，CSEE领域的应用型研究看来越来
越误入歧途？ |
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c******e
发帖数: 350 | 49 We had an accepted offer for a house. It's an estate sale.
I heard that it can be check for whether anyone died in a property by
ordering a death certificate.
Does anyone know how to get a copy of death certificate of previous owner
else?
Or is there any other way to check whether the previous owner died in his
house?
Thanks a lot! |
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g**********8
发帖数: 86 | 50 记得很小的时候开始有fear of death的感受。十来岁的时候有次半夜醒来，摸到自己
的枕骨突起的地方，想着自己不会得了脑瘤了吧，在那霎那间心如擂鼓的恐惧和孤独感
，记忆犹新。自己选择学医，是否多少有这方面潜在的影响，我不知道。
我一直觉得只有postpartum的女性才会有fear of death的最深沉最真实的感受。生理
上激素的巨大改变，心理的因素，交杂在一起，让我们对生命有了更深沉的体会和感受。
在医院工作，经常面对病床上垂危的患者，让人不得不去面对现实中经常选择去逃避的
问题。What will happen when our lives end? What is it like not to feel and
sense anymore eternally? How will the world be while it has no relationship
with me anymore? Isn't scary to know nothing about the world for ever and
the individual life of ourselves... 阅读全帖 |
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