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全部话题 - 话题: colonies
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c******z
发帖数: 1230
1
Garden的全景为什么让人压力大?不仅仅是因为植物本身,还有花园和房屋以及周围环
境的融合。真正赏心悦目的花园不一定要非常恢弘、工整,而是适度的、成熟的,并且
和房屋、环境融洽的。
我所居住的城市在1900-1950年代曾经辉煌,我曾经居住过的这条街道上,都是一些保
存完好的1920's Colonials,每家每户都有精心耕耘的花园。从大路一转进入这条静谧
街道,每家每户的房屋和花园都有非常浓厚的architechture detail和历史积淀的感觉
,田园气息浓郁。
发几张图,和大家一起欣赏。
m********r
发帖数: 124
2
来自主题: Biology版 - colony PCR 牛人过来指点下
版上牛人多,特来问个问题:
PCR总是令人烦恼,国内的时候就遇到过,现在又出来折腾我了:
我是想分别扩e.coli两个蛋白的两个同源domain,都很小,200bp的样子,但总是得不到
,都只有引物二聚体。我不觉得是引物的问题,因为两组引物都失败了,而且以前没有
遇到过这个问题。我到觉得是模板的问题。我直接用的colony,然后用PCR的那个95度
10Min,理论上认为能裂菌。我在想是裂菌不充分呢?还是我开始加的菌落太多了?我
一直担心模板不够,挑个菌落在tube里twirl了很久。或者裂菌之后就是问题比较多,
我应该尝试的先制备chromosome再扩增呢?太头大了,牛人过来指点下吧
s********n
发帖数: 2939
3
来自主题: Biology版 - colony PCR 牛人过来指点下
如果还是不行,你可以试试这个protocol:
1. 挑一个colony加到5 ul solution 2 (plasmids prep);
2. pipette几次确保细胞裂解了(可以加热到100C for 2 min);
3. 加入0.5 ml 10 mM Tris-HCl (pH 8.0) or H2O;
4. 15,000xg for 5 min;
5. 用上清作为模板(0.5 or 1 ul);
6. 这个上清可以保存在-20C供下次再用。
H****s
发帖数: 301
4
来自主题: Biology版 - Yeast Colony PCR protocol
各位同学,谁有Yeast Colony PCR protocol分享一下? 现在用的这个太费时间,费精力,
并且要好几次才能做出来.谢谢啦.
B***v
发帖数: 113
5
来自主题: Biology版 - QuikChange,没有colony,咋办?
我用QuikChange做mutaion,成功率70%的样子。其余30%没有colony,
该咋办,有经验的大师给指点一下。
M******g
发帖数: 152
6
来自主题: Biology版 - QuikChange,没有colony,咋办?
Try NEB Q5® Site-Directed Mutagenesis Kit https://www.neb.com/products
/e0554-q5-site-directed-mutagenesis-kit). It is much much better than the
quick change. They also have a web tool to help you design the primers: http://nebasechanger.neb.com/. Very convenient.
Make sure to use their comp cells. if you use comp cells from other
companies, dilute your final rxn (1:3), then do the transformation.
If your plasmid is big such as 8 kb, I normally double the denaturing time
as NEB sugge... 阅读全帖
m******o
发帖数: 8504
7
来自主题: Biology版 - QuikChange,没有colony,咋办?
我们都是直接用Q5 polymerase,不用KIT,就是再普通PCR的基础上稍微调调参数就可
以了。
然后挑5-6个colonies,能搞到1-2个正确的就可以了
w********a
发帖数: 324
8
来自主题: Biology版 - 【长段colony PCR】用什么酶?
请教大家一个问题:
做长段的colony PCR 用于检测阳性克隆,不要求高保真。PCR size 大于3 kb (在3-
6kb)。大家都用什么PCR酶检测啊?之前一直用NEB的 longAmp。效果不错,但是价格
太贵了。不知道有什么便宜点的替代产品。 谢谢!
g*****n
发帖数: 250
9
来自主题: Biology版 - 【长段colony PCR】用什么酶?
做colony PCR用 Taq就行了。好奇问一下,为什么非要PCR 3-6kb? 设计引物PCR短点的
不行吗?
w********a
发帖数: 324
10
来自主题: Biology版 - 【长段colony PCR】用什么酶?
是这么个理。但是3kb以上的PCR很多时候还是需要的。
请问你们的Taq是那个公司买的?你们在什么菌里面做colony PCR?谢谢!
p**t
发帖数: 36
11
来自主题: Science版 - Re: 为什么colonies有大有小呢?
Are those small colonies close to each other?
And the big ones are isolated?
Anyway it's not a big deal, as long as there's no contaminations.
s********n
发帖数: 455
12
来自主题: _MD_House_Buyers版 - a nice 3-level colonial in silver spring
3-level colonial with 2-car garage in silver spring, on Hawkesbury Lane,
20904
market value 435k. You can buy for 370k or less if you can act quickly.
这个房子不在市场上,见我以前发的帖子。我会陆续贴出好的房源,都远远低于市场价。
http://www.mitbbs.com/clubarticle_t/MD_House_Buyers/28001.html
w*******y
发帖数: 60932
13
This deal is shipped and zero tax. I sent this a gift to my parents and also
got some stuff shipped to me. Total with multiple shipping addresses was $
19.09!
Go to Swiss Colony website here
Look up Postpaid Holiday Bars & Logs in the search bar. Price is $19.95.
Decide who to send it to and add to cart. Since postage is included, this
really is a super good way to get the free chocolates and/or nuts!
Now the fun part! Decide which two freebies you want and enter the code into
the search bar:
FR... 阅读全帖
w*******y
发帖数: 60932
l********y
发帖数: 178
15
(It would take) 300 years of colonialism. In 100 years of colonialism, Hong
Kong has changed to what we see today. With China being so big, of course it
would require 300 years as a colony for it to be able to transform into how
Hong Kong is today. I have my doubts as to whether 300 years would be
enough.
---Xiaobo Liu, Open Magazine, November 1988
The above “300 years of colonialism” theory for China was actually
proposed by Xiaobo Liu, who was awarded the 2010 Nobe... 阅读全帖
D*********n
发帖数: 279
16
来自主题: Military版 - American History
Pre-Columbian Americans: (pdf) Spreading from Alaska to Tierra del Fuego,
America's first immigrants cultivated maize, developed sophisticated
calendars and used zeros, built temples and pyramids without the use of
wheels, but were helpless against the disease and weapons of the Europeans.
Chart: 16th & 17th Century European Colonizing Efforts (pdf)-Five European
nations (Spain, Portugal, The Netherlands, France, and England) played
major roles in opening up the New World to priests, treasure-... 阅读全帖
D*********n
发帖数: 279
17
来自主题: Military版 - Native Americans in the United States
http://en.wikipedia.org/wiki/Native_Americans_in_the_United_Sta
Native Americans in the United States
From Wikipedia, the free encyclopedia
Jump to: navigation, search
"American Indian" redirects here. For other indigenous peoples, see
Indigenous peoples of the Americas and other geographic regions. For
Americans from South Asia, see Indian American.
This article may be too long to read and navigate comfortably. Please
consider splitting content into sub-articles and/or condensing it. (January
2... 阅读全帖
a*****1
发帖数: 3817
18
来自主题: USANews版 - trump懵逼了,trump轮集体被打脸
自己去看美国宪法
United States Constitution
From Wikipedia, the free encyclopedia
Jump to: navigation, search
United States Constitution
Constitution of the United States, page 1.jpg
Page one of the original copy of the Constitution
Created
September 17, 1787
Ratified
June 21, 1788
Date effective
March 4, 1789; 227 years ago
Location
National Archives,
Washington, D.C.
Author(s)
Philadelphia Convention
Signatories
39 of the 55 delegates
Purpose
To replace the Articles of Confederation (177... 阅读全帖
a*****1
发帖数: 3817
19
来自主题: USANews版 - trump懵逼了,trump轮集体被打脸
自己去看美国宪法
United States Constitution
From Wikipedia, the free encyclopedia
Jump to: navigation, search
United States Constitution
Constitution of the United States, page 1.jpg
Page one of the original copy of the Constitution
Created
September 17, 1787
Ratified
June 21, 1788
Date effective
March 4, 1789; 227 years ago
Location
National Archives,
Washington, D.C.
Author(s)
Philadelphia Convention
Signatories
39 of the 55 delegates
Purpose
To replace the Articles of Confederation (177... 阅读全帖
L*********s
发帖数: 3063
20
来自主题: Military版 - 转一篇知乎上的文章[中英文版]
This is the article I wrote yesterday, first posted Chinese.
是啊,中国共产党是一个邪教。
在70多年前,中国处于长期半分裂状态,美英法德俄意日等在中国都有区域代理人。中
华民国中央政府受制于不与国家配合的地方军阀,国家连铁路在地方省份上的口径都无
法统一时,中国孱弱无力。我们作为一个延续了几千年的政治实体,却被西方看作是下
等人,愚昧的低级动物时,是中国共产党,通过内战结束中国的这个状态。
所以,在除台湾外的基本的国家统一下,中共有了它的第一个原罪:中央集权。
在60多年前,除了中国仅承认的西方殖民地香港和澳门,两个不属于中华人民共和国的
城市土地外,中共驱逐了所有的境外资本势力、特工与军队。在1956年。中国共产党迫
使自己的老师,苏联共产党,从中国辽宁省大连市的旅顺特别区撤军的时候,不惜与全
世界两大霸权国家美国和苏联同时交恶,被全世界进行经济封锁,中共才终于结束了作
为列强的傀儡而存在。无论是西方还是苏联。在苏联的长波电台和联合舰队倡议被中共
拒绝后,中苏彻底交恶,紧接着就是中共备受诟病的三年困难时期饿死成百上千万人。... 阅读全帖
l****z
发帖数: 29846
21
http://online.wsj.com/articles/accounting-firm-must-face-fdic-s
A federal judge said PricewaterhouseCoopers LLP must face the Federal
Deposit Insurance Corp.'s $1 billion lawsuit that alleges the accounting
firm failed to catch the massive fraud that brought down Colonial Bank, one
of the largest bank collapses in U.S. history.
Judge W. Keith Watkins of the U.S. District Court in Montgomery, Ala., said
Tuesday that the FDIC's theory that the auditor's failure to uncover the
fraud was "plausible"... 阅读全帖
s*****n
发帖数: 617
22
来自主题: Colorado版 - [TR] Crestone Traverse and Humboldt
[TR] Crestone Traverse and Humboldt - South Colony Lake 14er Sweep
爬这几个山头是年初就和山友T计划好的,因为T的工作性质,请假必须提前好几个月通
知,我们就把日子定在奥运开幕前的那个周末。至于到时候天气如何,只能撞大运了,
反正爬山本来就是靠天吃饭,三天之后的气象预报就不怎么靠谱,索性来个拣日不如撞
日。行程如下:
周四早退数小时,和T汇合后开车至South Colony Lake Upper TH,步行约三迈半到
South Colony Lake,在11,500’左右扎营。
周五从Red Gully上Crestone Peak,然后 traverse到Crestone Needle;
周六上Humboldt,顺便拿下边上一个centennial 13er Columbia Point,下山回家。
先说一下approach,以前如果你有4WD的话可以一直开到离湖边一迈的地方,一到周末
就热闹无比,除了爬山的,还有钓鱼、露营、狂欢的各色人等。近年来ZF寅吃卯粮,入
不敷出,林业局(Forest Service)表示压力... 阅读全帖
s*****n
发帖数: 617
23
来自主题: Outdoors版 - [TR] Crestone Traverse and Humboldt
[TR] Crestone Traverse and Humboldt - South Colony Lake 14er Sweep
爬这几个山头是年初就和山友T计划好的,因为T的工作性质,请假必须提前好几个月通
知,我们就把日子定在奥运开幕前的那个周末。至于到时候天气如何,只能撞大运了,
反正爬山本来就是靠天吃饭,三天之后的气象预报就不怎么靠谱,索性来个拣日不如撞
日。行程如下:
周四早退数小时,和T汇合后开车至South Colony Lake Upper TH,步行约三迈半到
South Colony Lake,在11,500’左右扎营。
周五从Red Gully上Crestone Peak,然后 traverse到Crestone Needle;
周六上Humboldt,顺便拿下边上一个centennial 13er Columbia Point,下山回家。
先说一下approach,以前如果你有4WD的话可以一直开到离湖边一迈的地方,一到周末
就热闹无比,除了爬山的,还有钓鱼、露营、狂欢的各色人等。近年来ZF寅吃卯粮,入
不敷出,林业局(Forest Service)表示压力... 阅读全帖
t******g
发帖数: 3641
24
http://www.neighborhoodcats.org/HOW_TO_RELEASING_FIV_FELV_POSIT
我喜欢最后那段的一句话:“it's about improving the situation, not about
making it perfect. ”
希望和在帮助小动物的路上还在犹豫的宠友们一起共勉。我不会再犹豫是不是给猫找到
了perfect的家,只要能达到threshold的,就比他们在外面流浪强。这就是improving
。但是如果我因为不忍心,不去做,那么他们仍然还是在流浪,有可能明天后天就不在
了……
Releasing FIV/FeLV Positive Cats
Neighborhood Cats opposes euthanizing any feral cat simply because he or she
tests positive for FIV (feline immuno-deficiency virus) or FeLV (feline
leukemia virus). If the cat shows no act... 阅读全帖
b**********8
发帖数: 349
25
来自主题: Biology版 - 细胞稳定转染求助
新手,最近在做一个质粒的稳定转染至肝癌细胞,大概步骤如下,6孔板中转染,24小
时后按1:50的比例传至10cm dish中,(大约1~2万个细胞),再等24小时开始加药筛选
,没隔3天换液维持,至第9天前后开始出现大量细胞死亡,并出现镜下明显可见的
colony,至第12天左右出现肉眼下明显可见的colony,于是用黄色tips直接在肉眼下挑
取单个colony,分别转移至96孔板中培养,并逐渐放大至24孔板,然后进行鉴定。至今
挑了大概40个colony,仅有一个阳性,但是继续放大培养两代后阳性反应突然丢失。
现在我有两个问题,
1)阳性反应突然丢失的原因可能是什么?细胞不纯?阳性细胞被其他细胞取代?筛选
出colony并放大培养的最初几代过程中,是否一定不能中断选择药物?哪怕只有两三天
的中断?
2)在网上翻了些帖子,发现有的人会在挑出单个colony培养贴壁后在做有限稀释,挑
取单克隆,这样做是不是会是拿到真阳性克隆的机会更大?可不可以按照这样的思路来
做,先用我上面的挑取,培养并鉴定出一批(视运气好坏多少不等)有阳性反应的
colony(姑且认定这个colony里面含有我想要... 阅读全帖
k********k
发帖数: 5617
26
来自主题: Military版 - 惊讶于德国人的英语能力
http://news.xinhuanet.com/ziliao/2004-06/24/content_1545030.htm
http://lechinois.ca/agenda/bethune/bethune_memoire.htm
纪念白求恩
毛泽东
(一九三九年十二月二十一日)
http://lechinois.ca/agenda/bethune/bethune_memoire.htm
Mao Tse-tung
In Memory of Norman Bethune
December 21, 1939
http://lechinois.ca/agenda/bethune/bethune_memoire.htm
Mao Zedong
À la mémoire de Norman Bethune
(21 décembre 1939)
http://www.marxists.org/espanol/mao/escritos/NB39s.html
Mao Tse-tung
En Memoria de Norman Bethune
21 de diciembre de 19... 阅读全帖
k********k
发帖数: 5617
27
http://news.xinhuanet.com/ziliao/2004-06/24/content_1545030.htm
http://lechinois.ca/agenda/bethune/bethune_memoire.htm
纪念白求恩
毛泽东
(一九三九年十二月二十一日)
http://lechinois.ca/agenda/bethune/bethune_memoire.htm
Mao Tse-tung
In Memory of Norman Bethune
December 21, 1939
http://lechinois.ca/agenda/bethune/bethune_memoire.htm
Mao Zedong
À la mémoire de Norman Bethune
(21 décembre 1939)
http://www.marxists.org/espanol/mao/escritos/NB39s.html
Mao Tse-tung
En Memoria de Norman Bethune
21 de diciembre de 19... 阅读全帖
k********k
发帖数: 5617
28
来自主题: WaterWorld版 - 认为英语口语好的试下这些发音
Many people speak English and Mandarin.
How many people who speak Mandarin (and English) can speak fluently a second
foreign language, such as French, German, Spanish, Russian, Italian,
Japanese, Korean, Arabic, and others?
It is hard to pronounce English (particularly British English) correctly.
It is probably even harder to pronounce (Parisian) French correctly.
Please try to read aloud any of the following versions in a second foreign
language: French, Spanish, Italian, and German.
Russian is... 阅读全帖
k********k
发帖数: 5617
29
来自主题: Translation版 - 《紀念白求恩》
http://news.xinhuanet.com/ziliao/2004-06/24/content_1545030.htm
http://lechinois.ca/agenda/bethune/bethune_memoire.htm
纪念白求恩
毛泽东
(一九三九年十二月二十一日)
http://lechinois.ca/agenda/bethune/bethune_memoire.htm
Mao Tse-tung
In Memory of Norman Bethune
December 21, 1939
http://lechinois.ca/agenda/bethune/bethune_memoire.htm
Mao Zedong
À la mémoire de Norman Bethune
(21 décembre 1939)
http://www.marxists.org/espanol/mao/escritos/NB39s.html
Mao Tse-tung
En Memoria de Norman Bethune
21 de diciembre de 19... 阅读全帖
a**u
发帖数: 8107
30
☆─────────────────────────────────────☆
boximiya (boxi) 于 (Wed Jan 13 07:14:29 2010, 美东) 提到:
海地发生里氏7级地震,造成巨大人员伤亡
不知道是不是40天序幕的拉开,心情沉重,默哀
☆─────────────────────────────────────☆
boximiya (boxi) 于 (Wed Jan 13 07:18:51 2010, 美东) 提到:
衷心希望说我造谣的同学是对。
☆─────────────────────────────────────☆
bdbd (kakali) 于 (Wed Jan 13 07:26:29 2010, 美东) 提到:
地球肯定不会毁灭的,因为小青说它要毁灭。
明白啦吧?
类比:芙蓉姐姐肯定死不了,如果宋祖德说她要自杀。

☆─────────────────────────────────────☆
ray (百川) 于 (Wed Jan 13 08:35:15 2010, 美东) 提到:
不是说地球会... 阅读全帖
T**********t
发帖数: 1604
31
来自主题: Biology版 - strange problem
How did you choose your colony to pick?
How much volume of LB did you inoculate with a single colony and what type
and concentration of antibiotics are you using?
How long did you grow your LB culture? What temperature and speed?
Some general thoughts without any details regarding the above questions.
1)pour fresh LB plates, make fresh LB media
2) repeat ligation and transformation, make sure to include positive and
negative control
3) check plates to make sure you have healthy colonies and your... 阅读全帖
E*****m
发帖数: 25615
32
【 以下文字转载自 TrustInJesus 讨论区 】
发信人: Eloihim (真神), 信区: TrustInJesus
标 题: 清教徒(加爾文信徒)主張宗教自由嗎? 請所長研究一下。
发信站: BBS 未名空间站 (Thu Nov 24 08:30:16 2011, 美东)
清教徒確實主張自己有宗教自由,但是不主張別人可以有。
對於貴格教派,清教徒主張直接吊死, 而且也確實吊死了一些。
最後還要英國國王派人過來確保貴格派有宗教自由,防止清教徒
壓迫他們。
這種精神有什麼好繼承的?有什麼好說嘴的?
這板的加爾文信徒就是繼承這種精神的。
參考資料
http://en.wikipedia.org/wiki/Puritan#Opposition_to_Quakerism
The Puritans of the Massachusetts Bay Colony were the most active of the New
England persecutors of Quakers, and the persecuting spirit was shared by
the Pl... 阅读全帖
E*****m
发帖数: 25615
33
清教徒確實主張自己有宗教自由,但是不主張別人可以有。
對於貴格教派,清教徒主張直接吊死, 而且也確實吊死了一些。
最後還要英國國王派人過來確保貴格派有宗教自由,防止清教徒
壓迫他們。
這種精神有什麼好繼承的?有什麼好說嘴的?
這板的加爾文信徒就是繼承這種精神的。
參考資料
http://en.wikipedia.org/wiki/Puritan#Opposition_to_Quakerism
The Puritans of the Massachusetts Bay Colony were the most active of the New
England persecutors of Quakers, and the persecuting spirit was shared by
the Plymouth Colony and the colonies along the Connecticut river.[32] In
1660, one of the most notable victims of the religious intolerance was
English... 阅读全帖
t****n
发帖数: 2601
34
俄国批评文章
The Nobel Peace Prize, and an Instrument of Western Power
11.10.2010
Pages: 123By Peter Baofu, Ph.D.
Contrary to the China-bashing coverage by mainstream Western media (which
also controls much of global media), the award of the 2010 Nobel Peace Prize
to Liu Xiaobo, an imprisoned Chinese (convicted of violating state laws),
does not promote world peace and prosperity in the long run, for seven major
reasons as explained below.
(1) The first reason is that, contrary to the China-bashing t... 阅读全帖
o**1
发帖数: 6383
35
☆─────────────────────────────────────☆
aben (hehe) 于 (Sat Oct 16 23:52:21 2010, 美东) 提到:
前两年诺贝尔和平奖,不论是戈尔,还是obama,国际上各大媒体
都有非常明显的争议性。
只有这次刘晓波,国际上各大媒体,不论左右,conservative还是liberal
一边倒的祝贺刘晓波骂TG,貌似从来没有那么一致过。
说老实话,我本来以为以TG这些年存的那么多外汇储备,收买一两
个所谓“国际友人”,一两个有点影响外国媒体骂骂诺贝尔委员会,
应该还是做得到的,但是到现在都一个多礼拜了,只有一个从来没有
听说过的什么“挪威教授”,还有个美国老年愤青在自己blog的博文
里面指责诺贝尔(人家即使指责诺贝,也没有说TG一个好字),TG喉舌
为了增强影响力,仍是造谣给人家戴了个“CNN VP”的头衔,贻笑大方
难怪啊,菌斑EUV等几个著名五毛这个礼拜都发疯了。
☆─────────────────────────────────────☆
mofia (Mofia) 于 (Sat Oct 16 ... 阅读全帖
c**i
发帖数: 6973
36
来自主题: History版 - Robert Morris
John Steele Gordon, This Rebel Came Armed With a Balance Sheet. Wall Street
Journal, Nov. 27, 2010.
http://online.wsj.com/article/SB10001424052748703514904575602370668905224.html
(book review on Charles Rappleye, Robert Morris: Financier of American
Revolution. Simon & Schuster, 2010
http://books.simonandschuster.com/Robert-Morris/Charles-Rappleye/9781416570912
)
Quote:
"in the case of the Revolution, Great Britain—the richest country in Europe
and the possessor of the most advanced financial sy... 阅读全帖
a*****1
发帖数: 3817
37
自己去看美国宪法
United States Constitution
From Wikipedia, the free encyclopedia
Jump to: navigation, search
United States Constitution
Constitution of the United States, page 1.jpg
Page one of the original copy of the Constitution
Created
September 17, 1787
Ratified
June 21, 1788
Date effective
March 4, 1789; 227 years ago
Location
National Archives,
Washington, D.C.
Author(s)
Philadelphia Convention
Signatories
39 of the 55 delegates
Purpose
To replace the Articles of Confederation (1777)
This articl... 阅读全帖
c****6
发帖数: 1550
38
来自主题: Florida版 - 佛州中国店一览
前言:
fanccy网友说的对,我这里偷懒了。活动搞了,但反馈上来的信息没有再次整理汇
总,要检讨。
知错就改,最近有点时间,准备将版上有用的信息分门别类,汇总成单篇,以方便
大家来查询。先从中国店开始,然后就是餐馆,旅游点。。。,咱一个个来。
Gainesville地区
1 店名: Chun Ching(重庆)
地理位置: 418 NW 8th Ave Gainesville, FL 32601
营业时间: Mon-Sat 9:30 am - 7:30 pm, Sun 12 pm - 6 pm
联系电话: (352) 376-5885
此由cuv(螂王)提供
2 店名: 瑞福
地理位置: NW 13 st 23Ave附近的大plaza里面
营业时间:?
联系电话: (352) 3713086
此由dpg (肖浪--君子不党)提供
3 大东
Oriental 88
Address:2110 Southwest 34th Street
Gainesville, FL 32607
(352) 378-8583
此由chentia (tia)提供
4 八月
1236 NW 21... 阅读全帖
c****6
发帖数: 1550
39
来自主题: Florida版 - 佛州中国店一览
前言:
fanccy网友说的对,我这里偷懒了。活动搞了,但反馈上来的信息没有再次整理汇
总,要检讨。
知错就改,最近有点时间,准备将版上有用的信息分门别类,汇总成单篇,以方便
大家来查询。先从中国店开始,然后就是餐馆,旅游点。。。,咱一个个来。
Gainesville地区
1 店名: Chun Ching(重庆)
地理位置: 418 NW 8th Ave Gainesville, FL 32601
营业时间: Mon-Sat 9:30 am - 7:30 pm, Sun 12 pm - 6 pm
联系电话: (352) 376-5885
此由cuv(螂王)提供
2 店名: 瑞福
地理位置: 1349 NW 23rd Ave
Gainesville, FL 32605
营业时间:10:00am -9:00 pm
联系电话: (352) 371-3085
此由dpg (肖浪--君子不党)提供
3 大东
Oriental 88
Address:2110 Southwest 34th Street
Gainesville, FL 32607
(352) 378... 阅读全帖
b****l
发帖数: 132
40
做了功课,收集了亚洲超市地址一览表, 回馈大家,欢迎大伙更新,
South Fort Lauderdale
金昌超市 电话:954-689-8192 地址:6887 Strilling Rd. Davie, FL 33314
North Fort Lauderdale
中国城超市 new york mart http://www.newyorkmarts.com/product/showproduct.php?lang=cn&id=26
foodtown 6431 Stirling Rd, Davie, FL 33314 (954) 797-7790
foodtownflorida.com
东方市场 电话:954-340-8828 地址:2365 University Dr. Coral Springs, FL
33065
West Palm Beach
西贡超级市场(Lake Park) 电话:407-863-6467 地址:724 Park Ave. Lake Park,
FL 33403
Orlando
丰田超级市场 电话:407-816-6700 地址:9421... 阅读全帖
c**i
发帖数: 6973
41
来自主题: Literature版 - Robert Morris
John Steele Gordon, This Rebel Came Armed With a Balance Sheet. Wall Street
Journal, Nov. 27, 2010.
http://online.wsj.com/article/SB10001424052748703514904575602370668905224.html
(book review on Charles Rappleye, Robert Morris: Financier of American
Revolution. Simon & Schuster, 2010
http://books.simonandschuster.com/Robert-Morris/Charles-Rappleye/9781416570912
)
Quote:
"in the case of the Revolution, Great Britain—the richest country in Europe
and the possessor of the most advanced financial sy... 阅读全帖
G******e
发帖数: 9567
42
Walter L. Sargent, Assistant Professor of History, University of Maine
Farmington
http://iweb.tntech.edu/kosburn/history-201/Puritans%20&%20Indians.htm
Indians and Puritans
A. Background
1. Indians of New England included the Abenaki, Pawtucket, Massachuset,
Narragansett. Pequot, and Wampanoag tribes
2. In 1616 an epidemic wiped out 3/4 of the New England Indian population,
so that Puritans landed in an area where Indians' ability to resist was
greatly weakened
3. In 1633-34 another smallpox epi... 阅读全帖
c*********d
发帖数: 9770
43
来自主题: History版 - -Where the East and the West meet
By Ishaan Tharoor April 28, 2015
There's no place on Earth where East and West, cardinal directions, don't
meet.
But, over the centuries, East and West — particularly the East in the minds
of people in the West — have become terms loaded with cultural freight.
For a long time in the European imagination, the divide between these
spheres centered on where Christendom ended and the lands of Islam began.
The history of colonialism both reinforced and reconfigured these divides.
The map below is Wor... 阅读全帖
C***Y
发帖数: 1323
44
Many people, in regards to Japan's troublemaking on the Diaoyutai Islands,
imagine that America wants to provoke confrontation between Japan and China.
Who doesn't know that the Japanese government wants to strike China as a
display for America? Japanese people are extremely disgusted and
dissatisfied with American long-term occupation, especially the military
bases on Okinawa on which the Japanese government has failed to keep their
promises to the people. The U.S. occupation of Japan has deepe... 阅读全帖
s*********8
发帖数: 901
45
David Cameron on Wednesday became the first serving British prime minister
to voice regret about one of the bloodiest episodes in colonial India, a
massacre of unarmed civilians in the city of Amritsar in 1919.
The killings, known in India as the Jallianwala Bagh massacre, were
described by Mahatma Gandhi, the father of the Indian independence movement,
as having shaken the foundations of the British Empire. A group of soldiers
opened fire on an unarmed crowd without warning in the northern Indi... 阅读全帖

发帖数: 1
46
来自主题: Military版 - 12 月26日 可能的真相
12月26日 事实的真相
以前我们分析过,Augusta, GA 只是这个人体基地的一个点,而且我们也追踪到还有一
个点在加州。那么经过更多的分析,显示事实的真相可能是如此。这些地下通道是很久
以前就存在的,是为了战争或避难用的,后来在南北战争中,又充当奴隶逃跑到北方的
通道。那么在后来的后来,就被一些别有用心的人充当了非法活动的场地。而到了80年
代后,日本人就加入进来,把其改造成了一个军事,人体实验基地,成了报复美国的一
种手段。当然,我也强调历史和解,仇恨和解,但要在正确的认知,悔改下的和解。如
果一边在屠杀受害者,一边高谈和解,并千方百计掩盖事情真相的曝光,这不是悔改的
表现,最多是转移了屠杀的目标,我们不能原谅罪恶势力彼此勾结下的和解。根据分析
,这个基地的入口就在Deerfield, ILLinois. 世界每一个人都是这个基地的受害者,
都是这些大药厂的受害者,受害者以亿为单位来计算。你们很可能都在不知不觉中受到
了他们的伤害,请把信息传达到世界每一个角落,让我们一起携手和世界上最邪恶的势
力做顽强的斗争,他们现在还在屠杀无辜的受害者,每天在我家里杀人。请你们和我站
在... 阅读全帖

发帖数: 1
47
https://www.quora.com/Did-Hong-Kong-have-to-pay-money-to-Britain-every-year-
when-it-was-still-a-British-colony
Did Hong Kong have to pay money to Britain every year when it was still a
British colony?
Oh Yes, a lot.
Britain did not levy any tax on Hong Kong. It worked the other way round.
Remember, to the British, the Opium War was not a war on sovereignty but a
war on trade. You never tax a colony. You trade with it or simply put, you
rob it, with or without opium.
Military Expenses. Hong Kong... 阅读全帖
b********n
发帖数: 38600
48
来自主题: Military版 - 美国其它的原罪
America’s Other Original Sin
We have never reckoned with the bloody U.S. takeover of the Philippines, our

https://www.theamericanconservative.com/articles/americas-other-original-sin
-philippines/
1898 US Political Cartoon: U.S. President William McKinley is shown holding
the Philippines, depicted as a savage child, as the world looks on. The
implied options for McKinley are to keep the Philippines, or give it back to
Spain, which the cartoon compares to throwing a child off a cliff. (
Uncred... 阅读全帖
r*********t
发帖数: 4911
49
要表彰你查数据的行为。这是讨论的基础。
我回去也查了资料,我俩有一个共同的错误,那就是对殖民地时期的dgp盲从。有本书
叫做:Slavery and American Economic Development,这里面提到per capita GDP is
to some degree unhistorical in that it rrepresents the modern egalitarian
perspective. 书中后面解释了为什么dpg不适合来解释美国建国初国家经济。一个是奴
隶没有投票权,可是却要计算在dpg per capita里面。二个是gdp是当代的流动性概念
(你说你有一块钱产值,那必须我这边也认你是一块钱产值,也就是你的商品在我这里
也值一块钱),而建国时的国家经济很多是没什么流动性的(区域的价格都不同,实际
上没法用gdp来计算)。书中提到,财富(wealth),才是计算国家经济的更好方式。于
是呢,我就去查了美国当时的国民财富,我查到了亮点
第一,美国殖民地时期国民收入高于英国。In 1774 the American colonies had
aver... 阅读全帖
w****r
发帖数: 245
50
大侠,分类都分好了,求神签
# Basic Materials, Agricultural Chemicals
AGU Agrium Inc.
AVD American Vanguard Corp.
CAGC China Agritech Inc.
CF CF Industries Holdings, Inc.
CGA China Green Agriculture, Inc.
CMP Compass Minerals International
COIN Converted Organics Inc.
IPI Intrepid Potash, Inc.
MON Monsanto Co.
MOS Mosaic Co.
NOEC New Oriental Energy & Chemical
POT Potas... 阅读全帖
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