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发帖数: 773 | 1 Abstract
The global demand for food could double in another 40 y owing to growth in
the population and food consumption per capita. To meet the world’s future
food and sustainability needs for biofuels and renewable materials, the
production of starch-rich cereals and cellulose-rich bioenergy plants must
grow substantially while minimizing agriculture’s environmental footprint
and conserving biodiversity. Here we demonstrate one-pot enzymatic
conversion of pretreated biomass to starch through a nonnatural synthetic
enzymatic pathway composed of endoglucanase, cellobiohydrolyase, cellobiose
phosphorylase, and alpha-glucan phosphorylase originating from bacterial,
fungal, and plant sources. A special polypeptide cap in potato alpha-glucan
phosphorylase was essential to push a partially hydrolyzed intermediate of
cellulose forward to the synthesis of amylose. Up to 30% of the
anhydroglucose units in cellulose were converted to starch; the remaining
cellulose was hydrolyzed to glucose suitable for ethanol production by yeast
in the same bioreactor. Next-generation biorefineries based on simultaneous
enzymatic biotransformation and microbial fermentation could address the
food, biofuels, and environment trilemma.
http://www.pnas.org/content/early/2013/04/12/1302420110 | c*******n
发帖数: 1648 | 2 祝贺，院士圈里混了。开个公司圈钱吧。还有，老兄说说现在产业化的难点在哪里？ |
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