h*********g
发帖数: 18 | 1 我用maxiprep提取plasmid (2Kb)，跑胶得到2条带，还有些模糊的带，cut后也有几条
带，其中一条带是2kb, all the other bands are larger than the 2kb band。THe
restriction enzyme should only cut one site in the plasmid.Can I trust the
result? After that I will do the gel extraction. Why there are several bands
? Thanks. |
x********e
发帖数: 35261 | 2 看着不靠谱啊 最好测一下序
如果质粒有污染，后续实验都不靠谱
bands
【在 h*********g 的大作中提到】
: 我用maxiprep提取plasmid (2Kb)，跑胶得到2条带，还有些模糊的带，cut后也有几条
: 带，其中一条带是2kb, all the other bands are larger than the 2kb band。THe
: restriction enzyme should only cut one site in the plasmid.Can I trust the
: result? After that I will do the gel extraction. Why there are several bands
: ? Thanks.
|
s********x
发帖数: 472 | 3 跑胶两条带就有问题了，就算不是污染也是d na受损。
应该看到一条带就对了。 |
