j*****a
发帖数: 92 | 1 The insert should be 0.9 kb, which is correct on oagarose gel
electrophoresis analysis. However, after ligation, tranformation. Positive
transformants show plasmid (correct size( and insert (1.5 kb instead 0.9 kb)
. Then we expressed it and do not get the protein. Any suggstion? Thanks | q***7
发帖数: 144 | | g*****n
发帖数: 250 | 3 You should sequence the constructs, at least, to make sure that the ORF is
correct (with start and stop codons). |
|
