m********r
发帖数: 124 | 1 有没有人指点下SAXS这个东西是不是很有前途？似乎追求的是在溶液中看大分子的
低分辨动态变化。我PhD 期间NMR和 X-ray都在做，但是小的domain，在纠结薄厚是继
续深入做biophysics还是换到in vivo，比如single molecule FRET和SAXS比是不更有
前途？ |
G**********s
发帖数: 74 | 2 UIUC的taekjip ha是不是在做single molecule的FRET？ |
b******y
发帖数: 627 | 3 SAXS is just another biophysical technique, which can provide some useful
but limited information on your system of interest. Nobody should base his
career on such a small technique. |
l**********1
发帖数: 5204 | 4 pls focus on protein folding analysis by Solid NMR new method
References:
PMID 22522262
Nat Chem. 4:410-7. (2012)
protein fold in the solid phase via measurements of nuclear paramagnetic
relaxation enhancements (PREs) in several analogues of the protein of
interest containing covalently attached paramagnetic tags, without the use
of conventional internuclear distance restraints.
then from
the paucity of distance restraints >5 Å
to
B1 immunoglobulin-binding domain of protein G, for which ~230 longitudinal
backbone (15)N PREs corresponding to distances of
~10-20 Å were obtained.
web link:
http://www.ncbi.nlm.nih.gov/pubmed/22522262
or
PMID 22723345
PNAS 109:11095-100. (2012).
solid-state nuclear magnetic resonance (NMR) with (1)H detection under
ultrafast magic angle spinning (MAS).
http://www.ncbi.nlm.nih.gov/pubmed/22723345
【在 m********r 的大作中提到】
: 有没有人指点下SAXS这个东西是不是很有前途？似乎追求的是在溶液中看大分子的
: 低分辨动态变化。我PhD 期间NMR和 X-ray都在做，但是小的domain，在纠结薄厚是继
: 续深入做biophysics还是换到in vivo，比如single molecule FRET和SAXS比是不更有
: 前途？
|
m********r
发帖数: 124 | 5 Good to know. 不过现在做大蛋白的也很多 我倒是也有兴趣
【在 b******y 的大作中提到】
: SAXS is just another biophysical technique, which can provide some useful
: but limited information on your system of interest. Nobody should base his
: career on such a small technique.
|
