l******g
发帖数: 1145 | 1 某些原因需要quantify同一张图片上，两个部分的intensity，然后计算ratio（A/B)的
数值。
用什么软件比较方便？最好是免费的软件～ |
v***a
发帖数: 1242 | 2 ImageJ~
【在 l******g 的大作中提到】
: 某些原因需要quantify同一张图片上，两个部分的intensity，然后计算ratio（A/B)的
: 数值。
: 用什么软件比较方便？最好是免费的软件～
|
l******g
发帖数: 1145 | 3 呃？ImageJ如此万能？我再去钻研钻研，谢～～
【在 v***a 的大作中提到】
: ImageJ~
|
l******g
发帖数: 1145 | 4 再问一下，需要装哪个plug-in？
找到一个colour deconvolution plugin，但貌似比较适合HE和DAB staining.
【在 v***a 的大作中提到】
: ImageJ~
|
r**t
发帖数: 64 | 5 I also would like ask a question. After having all the bands selected, I
went to “gels ->Plot lanes”, but the pop-up window is vertical instead of
horizontal, making measurement of the peak areas impossible as I can’t see
where to draw the base line of a peak. What do I need to do so that the plot
will be horizontal showing each peak from left to right on the screen (
instead of from top to bottom). What plug-in do I need? Thanks. |
s*****j
发帖数: 6435 | 6 seems you can turn your image 90 degree.
of
see
plot
【在 r**t 的大作中提到】
: I also would like ask a question. After having all the bands selected, I
: went to “gels ->Plot lanes”, but the pop-up window is vertical instead of
: horizontal, making measurement of the peak areas impossible as I can’t see
: where to draw the base line of a peak. What do I need to do so that the plot
: will be horizontal showing each peak from left to right on the screen (
: instead of from top to bottom). What plug-in do I need? Thanks.
|
