o**a
发帖数: 86 | 1 【 以下文字转载自 Chemistry 讨论区 】
发信人: ouba (comfortably numb), 信区: Chemistry
标 题: how to block dna 3' OH
发信站: BBS 未名空间站 (Mon Apr 22 16:45:47 2013, 美东)
Anyone knows of a simple reaction to block the dna 3'OH from ligation to a
free 5'ppp? Thanks! | X***n
发帖数: 366 | 2 No way. If you don't mind add another nt to the 3'-end of DNA, you can
ligate a ddT to the 3'-end fist. | k****l
发帖数: 279 | 3 If you synthesize that DNA, just add a dideoxy C in the 3' end
【在 o**a 的大作中提到】
: 【 以下文字转载自 Chemistry 讨论区 】
: 发信人: ouba (comfortably numb), 信区: Chemistry
: 标 题: how to block dna 3' OH
: 发信站: BBS 未名空间站 (Mon Apr 22 16:45:47 2013, 美东)
: Anyone knows of a simple reaction to block the dna 3'OH from ligation to a
: free 5'ppp? Thanks!
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