do someone use a mammalian plasmids that have two promoters and could
express two SEPARATED RNAs? I need express one protein and one mRNA in the
cells with one plasmids. thanks.
l**********1 发帖数: 5204
2
pVITRO1-neo-mcs
neo (kanamycin/G418 resistance).
pVITRO1-MCS
Expression plasmids pVITRO1-MCS are developed mainly for in vitro studies.
pVITRO1-MCS allow the ubiquitous and constitutive co-expression of two genes
of interest.
pVITRO1-MCS plasmids can be stably transfected in mammalian cells and the
genes of interest are expressed at high levels.
pVITRO1-MCS plasmids carry two elongation factor 1 alpha (EF-1α) promoters,
from rat and mouse origins combined to the CMV and SV40 enhancers
respectively. pVITRO1-MCS plasmids are available with different selectable
markers that are active both in E. coli and mammalian cells.
pVITRO1-mcs plasmids contain two multiple cloning sites (MCS) for the
convenient cloning of your cDNAs of interest. http://www.invivogen.com/pvitro1-mcs
【在 g*********5 的大作中提到】 : do someone use a mammalian plasmids that have two promoters and could : express two SEPARATED RNAs? I need express one protein and one mRNA in the : cells with one plasmids. thanks.
