x******i
发帖数: 918 | 1 要是把体外养了一两个月的CELL PELLET植入老鼠皮下，能不能看到老鼠的血管慢慢长
到PELLET里面？还是会被老鼠吸收？有没有其他什么办法看血管生成？谢谢。 |
n***w
发帖数: 2405 | 2 1. study retinal development from P0.
2. your description is more like an in vivo matrigel plug assay.
http://link.springer.com/content/pdf/10.1385%2F1-59259-143-4%3A |
v*********d
发帖数: 382 | 3 intravital microscopy
http://www.surgery.wisc.edu/research/researchers-labs/cardiotho
【在 x******i 的大作中提到】
: 要是把体外养了一两个月的CELL PELLET植入老鼠皮下，能不能看到老鼠的血管慢慢长
: 到PELLET里面？还是会被老鼠吸收？有没有其他什么办法看血管生成？谢谢。
|
x******i
发帖数: 918 | 4 Not what I want. Thx.
【在 n***w 的大作中提到】
: 1. study retinal development from P0.
: 2. your description is more like an in vivo matrigel plug assay.
: http://link.springer.com/content/pdf/10.1385%2F1-59259-143-4%3A
|
x******i
发帖数: 918 | 5 I know about this technique. Way too complicated, time consuming and they
will charge a lot for their service. Not worth it. I just want a simple and
straightforward angiogenesis study. I know what to do now.
Thank you anyway.
【在 v*********d 的大作中提到】
: intravital microscopy
: http://www.surgery.wisc.edu/research/researchers-labs/cardiotho
|
Z******5
发帖数: 435 | 6 我们实验室以前搞过一个转基因的斑马鱼，血管全部用EGFP标记，打进去肿瘤细胞后，
可以活体看到血管向着肿瘤方向生长。
不知道有没有这样的老鼠。如果有的话，我也想用呢。 |
a**********2
发帖数: 3726 | 7 Does anyone else also feel that the way how LZ responded to other persons'
help is very uncomfortable? Is that very typical in bio field? |
c******m
发帖数: 503 | 8 I do. I think it's just him, especially it's online. In real life it does
not work this way.
【在 a**********2 的大作中提到】
: Does anyone else also feel that the way how LZ responded to other persons'
: help is very uncomfortable? Is that very typical in bio field?
|
a**********2
发帖数: 3726 | 9 Hopefully that is not how he talks with people in real life.
【在 c******m 的大作中提到】
: I do. I think it's just him, especially it's online. In real life it does
: not work this way.
|
y****y
发帖数: 123 | 10 老鼠有tie2GFP啊
不过活体不好看就是了
【在 Z******5 的大作中提到】
: 我们实验室以前搞过一个转基因的斑马鱼，血管全部用EGFP标记，打进去肿瘤细胞后，
: 可以活体看到血管向着肿瘤方向生长。
: 不知道有没有这样的老鼠。如果有的话，我也想用呢。
|
t******y
发帖数: 716 | 11 Fluorescein labeled Lycopersicon Esculentum (Tomato) Lectin |
A********e
发帖数: 23 | 12 你能告诉大家你现在知道的方法是什么吗？也算是服务大家，正如你上来请教大家一样。
and
【在 x******i 的大作中提到】
: I know about this technique. Way too complicated, time consuming and they
: will charge a lot for their service. Not worth it. I just want a simple and
: straightforward angiogenesis study. I know what to do now.
: Thank you anyway.
|
x******i
发帖数: 918 | 13 请有人不要那么敏感好不好，这只不过是在网上交流交流学术问题，直接了当一点怎么
了？而且不是都谢过了么？
我现在准备用一个土方法，作为一个ｅｎｇｉｎｅｅｒ，觉得能够说明问题已经足够了
。说出来不怕各位高手见笑，等我试过有效的话再上来和大家交流。
新年快乐！ |
