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Biology版 - 关于荧光显微镜
相关主题
单分子显微技术有解决细胞的自身荧光问题吗?求审稿-生物物理领域 (转载)
挖个坑:荧光显微镜在工业界应用如何,研究前景如何,欢迎讨论Optics, biophotonics and single molecule biophysics jobs
paper help求文献一个,谢谢啦!
现在公司还用fluorescence microscopy 么?single molecule fluorescence研究蛋白dynamics
paper help荧光显微镜试剂,大家用哪家的?
大家学得Single Molecule FRET(fluoresceance energy transfer)潜力如何,做这方面有没有前途?博士后职位
用什么方法可以证明一个homodimer,只有一个monomer和另一个蛋白结合Confocal对于荧光集团最大的分辨率?
荧光下的colocalization 是不是意味着contact以及interaction?trytophan fluorescence spectroscopy for protein structure
相关话题的讨论汇总
话题: flim话题: book话题: principles
进入Biology版参与讨论
1 (共1页)
h*****s
发帖数: 153
1
有人能推荐一些入门的关于荧光显微镜的书籍吗?FLIM的最好。。。
Thanks.
s******y
发帖数: 28562
2
http://www.microscopyu.com/

【在 h*****s 的大作中提到】
: 有人能推荐一些入门的关于荧光显微镜的书籍吗?FLIM的最好。。。
: Thanks.

p*l
发帖数: 1359
3
FLIM简介
http://www.olympusfluoview.com/applications/flimintro.html
Further reading:
1. Time domain method
Pictoquant application notes
http://www.picoquant.com/appnotes.htm
"Quantitative in vivo imaging of molecular distances using FLIM-FRET"
"Fluorescence Lifetime Imaging (FLIM)"
2. Frequency domain method (less commonly used in FLIM)
ISS application notes for single point measurement
http://www.iss.com/resources/research/application_notes/K2CH_Li
For FLIM microscope with the frequency domain method, you can search papers
by Philippe I.H. Bastiaens.
h*****s
发帖数: 153
4
Thanks so much~
s********n
发帖数: 248
5
mark
c*******0
发帖数: 190
6
Principles of Fluorescence Spectroscopy
by Joseph R. Lakowicz
p*l
发帖数: 1359
7
The book is an overkill for biologist. I use the book for 7 years now, but
still haven't got time to read every chapter of it. The first 5 chapters are
enough for a biology researcher who really wants to understand FLIM.
This handbook was written by a chemist. Examples in the book were often
taken from chemistry applications not biological applications. FLIM in
biological sample has its unique issues, which are not covered in this book.
For serious FLIM users, I suggest "FLIM Microscopy in Biology and Medicine
" , if one already finished the first 5 chapter of "Principles of
Fluorescence Spectroscopy".

【在 c*******0 的大作中提到】
: Principles of Fluorescence Spectroscopy
: by Joseph R. Lakowicz

c*******0
发帖数: 190
8
遇到老师了...班门弄斧了...Joseph书的4,5章对基本理论的解释和计算确实有很大帮
助。
乖乖去下载FLIM Microscopy in Biology and Medicine研习下先...

are
book.
Medicine

【在 p*l 的大作中提到】
: The book is an overkill for biologist. I use the book for 7 years now, but
: still haven't got time to read every chapter of it. The first 5 chapters are
: enough for a biology researcher who really wants to understand FLIM.
: This handbook was written by a chemist. Examples in the book were often
: taken from chemistry applications not biological applications. FLIM in
: biological sample has its unique issues, which are not covered in this book.
: For serious FLIM users, I suggest "FLIM Microscopy in Biology and Medicine
: " , if one already finished the first 5 chapter of "Principles of
: Fluorescence Spectroscopy".

h*****s
发帖数: 153
9

请问能给个电子书的链接吗?
我也想看着本书。

【在 c*******0 的大作中提到】
: 遇到老师了...班门弄斧了...Joseph书的4,5章对基本理论的解释和计算确实有很大帮
: 助。
: 乖乖去下载FLIM Microscopy in Biology and Medicine研习下先...
:
: are
: book.
: Medicine

c*******0
发帖数: 190
10
http://www.doc88.com/p-69355343087.html

【在 h*****s 的大作中提到】
:
: 请问能给个电子书的链接吗?
: 我也想看着本书。

h*****s
发帖数: 153
11

Thanks mate~
But这个只能在线看?

【在 c*******0 的大作中提到】
: http://www.doc88.com/p-69355343087.html
k****n
发帖数: 158
12
mark
c*******0
发帖数: 190
13
要1100的积分才能下载...凑合着看吧,觉得有用再去amazon买本二手的或者去学校图
书馆复印一下

【在 h*****s 的大作中提到】
:
: Thanks mate~
: But这个只能在线看?

1 (共1页)
进入Biology版参与讨论
相关主题
trytophan fluorescence spectroscopy for protein structurepaper help
谁参加过LFD workshop?大家学得Single Molecule FRET(fluoresceance energy transfer)潜力如何,做这方面有没有前途?
请教大家 关于显微镜用的moss leaflet 样品用什么方法可以证明一个homodimer,只有一个monomer和另一个蛋白结合
求帮助:细胞内荧光蛋白表达荧光下的colocalization 是不是意味着contact以及interaction?
单分子显微技术有解决细胞的自身荧光问题吗?求审稿-生物物理领域 (转载)
挖个坑:荧光显微镜在工业界应用如何,研究前景如何,欢迎讨论Optics, biophotonics and single molecule biophysics jobs
paper help求文献一个,谢谢啦!
现在公司还用fluorescence microscopy 么?single molecule fluorescence研究蛋白dynamics
相关话题的讨论汇总
话题: flim话题: book话题: principles