p****z 发帖数: 31 | 1 用某种药物刺激细胞,发现某个蛋白表达升高,但是该蛋白的表达不会随着药物浓度的
变化而变化,这其中的机制可能会是什么?希望大家提供意见参考一下,谢谢! | i***0 发帖数: 160 | 2 what assay did you use? Luciferase? Did you confirm the compound with a
different method, such as RT-PCR, or WB? Normally, people will assume a
compound with no dose response as a false positive. But it might also be
possible the compound is so potent that EC50 is lower than the lowest
concentration you tested. You might need to tell us more to let us help you
guess.
I wish your compound is a real inducer. Best luck! |
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