u**********d 发帖数: 573 | 1 效率怎么样?处理后再轻轻吹打能不能把组织或培养的细胞克隆打散成单细胞?
对细胞的伤害大不大?跟用trypsin比呢?
看millipore的介绍,可能这个溶液里的主要成分是一种受专利保护的EDTA,这种EDTA
不能进入细胞去影响胞内蛋白的功能了吗?
用过的请来说说。 | i***0 发帖数: 160 | 2 I have tried the Invitrogen enzyme-free cell dissociation solution,
dissociation is slower than trypsin, and milder. Better use that instead of
trypsin, if your assay is related to membrane proteins or cell membrane. | u**********d 发帖数: 573 | 3 多谢!我正是要做膜蛋白的分析。
of
【在 i***0 的大作中提到】 : I have tried the Invitrogen enzyme-free cell dissociation solution, : dissociation is slower than trypsin, and milder. Better use that instead of : trypsin, if your assay is related to membrane proteins or cell membrane.
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