C****r
发帖数: 281 | 1 用的是millipore的cell migration assay
thanks |
s******y
发帖数: 28562 | 2 To synchronize the cell, and to make them sensitive for chemotractant
【在 C****r 的大作中提到】
: 用的是millipore的cell migration assay
: thanks
|
k****o
发帖数: 589 | 3 请教下sunnyday，已经synchronized的细胞受到外源刺激后，会不会发生
desynchronization？ |
s******y
发帖数: 28562 | 4 在短时间内不会，时间一长就难说了。
比方说我熟悉的MEF，会有一定比例随机地进入细胞周期（而不是统一进入周期），
所以同步后过了一段时间就又是乱七八糟的了
【在 k****o 的大作中提到】
: 请教下sunnyday，已经synchronized的细胞受到外源刺激后，会不会发生
: desynchronization？
|
s****9
发帖数: 932 | 5 My understanding is that the complete medium with serum sometimes contains
the ligand that can bind to your chemotaxis receptor, which cause receptor
internalization and desensitization. Incubate them for 15-30min in medium
without serum or starve them overnight will resensize the migration and
increase receptor expression. If you are sure your culture medium does not
contain the ligand, you do not need to starve them.
【在 C****r 的大作中提到】
: 用的是millipore的cell migration assay
: thanks
|
k****o
发帖数: 589 | 6
这个现象背后的决定因素是随机过程吗？有没有什么好文章推荐啊，谢谢
【在 s******y 的大作中提到】
: 在短时间内不会，时间一长就难说了。
: 比方说我熟悉的MEF，会有一定比例随机地进入细胞周期（而不是统一进入周期），
: 所以同步后过了一段时间就又是乱七八糟的了
|
g*******3
发帖数: 2520 | 7 Both Sunnyday and your post make sense.
not
【在 s****9 的大作中提到】
: My understanding is that the complete medium with serum sometimes contains
: the ligand that can bind to your chemotaxis receptor, which cause receptor
: internalization and desensitization. Incubate them for 15-30min in medium
: without serum or starve them overnight will resensize the migration and
: increase receptor expression. If you are sure your culture medium does not
: contain the ligand, you do not need to starve them.
|
