由买买提看人间百态

boards

本页内容为未名空间相应帖子的节选和存档,一周内的贴子最多显示50字,超过一周显示500字 访问原贴
Biology版 - how to store cell lysate for a long time
相关主题
Re: degraded protein?How to test protein stability
问个FACS cell sorting的问题how to purify the degraded protein bands on the gel?
Troubleshooting for SDS-PAGE. Smear on low molecular proteins请问如何找到和protein degradation相关的所有基因?
anyone knows a way to irreversibly kill all kinases without denaturing other proteins?Re: 细胞长得太密会不会影响transfected protein的表达
做western的protein lysate浓度太低了大家都怎么浓缩?请教:quantitate cell surface protein level有什么好办法?
Protein lysate能放在cold room过夜吗?大家有碰到过Co-IP突然不work的情况吗?
【包子】用来做luciferase的cell lysate能够放在4度或者-20度保存2-3天[招聘] Protein&Cell招聘启事
请教一个harvest primary neuron cells的问题 (送包子)*_*请教E. coli 表达的蛋白90% 以上被degrade的掉,怎么避免?
相关话题的讨论汇总
话题: lysate话题: store话题: cell
进入Biology版参与讨论
1 (共1页)
m******t
发帖数: 109
1
after boiled and store -20?
r*******y
发帖数: 48
2
yes, that is what I usually do.
F********1
发帖数: 272
3
store in -80.
g*********5
发帖数: 2533
4
上次煮了后忘了放进冰箱,
bench上放了一周,还能用马?

【在 m******t 的大作中提到】
: after boiled and store -20?
r*******y
发帖数: 48
5
No, proteins will be degraded.

【在 g*********5 的大作中提到】
: 上次煮了后忘了放进冰箱,
: bench上放了一周,还能用马?

1 (共1页)
进入Biology版参与讨论
相关主题
请教E. coli 表达的蛋白90% 以上被degrade的掉,怎么避免?做western的protein lysate浓度太低了大家都怎么浓缩?
土问:RNA degradation能从OD260看出来吗?Protein lysate能放在cold room过夜吗?
is protein crystallography a good area for postdoc?【包子】用来做luciferase的cell lysate能够放在4度或者-20度保存2-3天
怎么定义 protein-protein interaction请教一个harvest primary neuron cells的问题 (送包子)*_*
Re: degraded protein?How to test protein stability
问个FACS cell sorting的问题how to purify the degraded protein bands on the gel?
Troubleshooting for SDS-PAGE. Smear on low molecular proteins请问如何找到和protein degradation相关的所有基因?
anyone knows a way to irreversibly kill all kinases without denaturing other proteins?Re: 细胞长得太密会不会影响transfected protein的表达
相关话题的讨论汇总
话题: lysate话题: store话题: cell