x******m
发帖数: 736 | 1 如果想从epigenomics的角度来研究human ips和es的不同，是不是很难？
问过一个教授，他说，要做人的话，必须ips和es来自于同一个人，这样才能排除
genomic DNA的variation，但这是不可能的，人取了es就不可能有ips了。
所以他说只能做mouse的stem cell。找同一strain的mouse，去es，再用somatic 诱导
出ips，这样比较他们的不同才有意义。
大家说说看，这种说法对吗？ |
a*******a
发帖数: 4233 | 2 同一strain的老鼠也有variation啊
我当时的想法是建一个es系、挑单克隆出来一半存了，
另一半诱导分化成终末分化细胞然后再ips
不过中间还有好几个技术问题
而且epigenome variation必然很大。。 |
x******m
发帖数: 736 | 3 多谢回复，中间好几个技术问题是什么？
我不是做stem cell，多谢指教 |
n***g
发帖数: 5027 | 4 分化之后，好多ES细胞很难去干净
【在 x******m 的大作中提到】
: 多谢回复，中间好几个技术问题是什么？
: 我不是做stem cell，多谢指教
|
n********k
发帖数: 2818 | 5 what are you thinking? from the way you asked, I am not sure you would want(
you are not ready, I mean, sorry) to jump into this hole/compitition...BTW,
tons of people are doing/thinking this kind of stuff if you are thinking of
what I am referring to here...I am not sure there is any valid approach,
nor valid significance...and then future continuation?
【在 x******m 的大作中提到】
: 如果想从epigenomics的角度来研究human ips和es的不同，是不是很难？
: 问过一个教授，他说，要做人的话，必须ips和es来自于同一个人，这样才能排除
: genomic DNA的variation，但这是不可能的，人取了es就不可能有ips了。
: 所以他说只能做mouse的stem cell。找同一strain的mouse，去es，再用somatic 诱导
: 出ips，这样比较他们的不同才有意义。
: 大家说说看，这种说法对吗？
|
x******m
发帖数: 736 | 6 你的英文。。。
我看了半天才懂你的意思，sorry。
我是做bioinfo的，也没想跳这个领域，只是在做数据分析的时候，跟做这个方向的教
授聊过，很好奇就上来问问。
want(
BTW,
of
【在 n********k 的大作中提到】
: what are you thinking? from the way you asked, I am not sure you would want(
: you are not ready, I mean, sorry) to jump into this hole/compitition...BTW,
: tons of people are doing/thinking this kind of stuff if you are thinking of
: what I am referring to here...I am not sure there is any valid approach,
: nor valid significance...and then future continuation?
|
n********k
发帖数: 2818 | 7 Good for you...as long as you figured it out, I am not gonna be sorry for my
english...BTW, I take no shame on my English no matter how you folks bash
me...I would have no time to do any experiments if I had to water either in
Chinese like Sunny or perfect English like Dr. Biologist...I am just
nevertthink and I don't care...this would be a first time and hopefully a
last time I would ever explain on my "wonderful" english
【在 x******m 的大作中提到】
: 你的英文。。。
: 我看了半天才懂你的意思，sorry。
: 我是做bioinfo的，也没想跳这个领域，只是在做数据分析的时候，跟做这个方向的教
: 授聊过，很好奇就上来问问。
:
: want(
: BTW,
: of
|
M*****n
发帖数: 16729 | 8 your English is OK, at least I can understand most of it.
a little suggestion, try to use less "would" or ..., coz, you talk like that
British King George V (or VI, I don't remember, you know what I mean, the movie"King's Speech". )
my
in
【在 n********k 的大作中提到】
: Good for you...as long as you figured it out, I am not gonna be sorry for my
: english...BTW, I take no shame on my English no matter how you folks bash
: me...I would have no time to do any experiments if I had to water either in
: Chinese like Sunny or perfect English like Dr. Biologist...I am just
: nevertthink and I don't care...this would be a first time and hopefully a
: last time I would ever explain on my "wonderful" english
|
n********k
发帖数: 2818 | 9 alright alright...I would have killed you,,,,,it is a secret,,,you know,,,,
hoho
that
movie"King's Speech". )
【在 M*****n 的大作中提到】
: your English is OK, at least I can understand most of it.
: a little suggestion, try to use less "would" or ..., coz, you talk like that
: British King George V (or VI, I don't remember, you know what I mean, the movie"King's Speech". )
:
: my
: in
|
m******5
发帖数: 1383 | 10 Lz is thinking in the absolutely right direction.... too bad there are at
least 2 nature cell
biology paper published in this topic |
|
|
n********k
发帖数: 2818 | 11 this is virtually no brain research in the field, check this history of
nuclear cloning, that's exactly what has happened there---Cool, equvilent of
ES---ops--they are different... Those studies are more for publications and
news as far as I am concerned...I don't mean they have no values though...
【在 m******5 的大作中提到】
: Lz is thinking in the absolutely right direction.... too bad there are at
: least 2 nature cell
: biology paper published in this topic
|
j****x
发帖数: 1704 | 12 a little bit over reacting ba...
my
in
【在 n********k 的大作中提到】
: Good for you...as long as you figured it out, I am not gonna be sorry for my
: english...BTW, I take no shame on my English no matter how you folks bash
: me...I would have no time to do any experiments if I had to water either in
: Chinese like Sunny or perfect English like Dr. Biologist...I am just
: nevertthink and I don't care...this would be a first time and hopefully a
: last time I would ever explain on my "wonderful" english
|
n********k
发帖数: 2818 | 13 not really...certainly not to LZ...I have shrugged off all the previous
nasty comments I have garnered over the ys:))) without a single word. For a
moment, i thought I would just took the chance to make a point:)))...
【在 j****x 的大作中提到】
: a little bit over reacting ba...
:
: my
: in
|
x******m
发帖数: 736 | 14 能不能告诉我是哪几篇paper？他们怎么造genetic identical的es和ips cells的？
【在 m******5 的大作中提到】
: Lz is thinking in the absolutely right direction.... too bad there are at
: least 2 nature cell
: biology paper published in this topic
|
M****7
发帖数: 214 | 15 GQ Daley 2010 "Epigenetic memory in induced pluripotent
stem cells"
【在 m******5 的大作中提到】
: Lz is thinking in the absolutely right direction.... too bad there are at
: least 2 nature cell
: biology paper published in this topic
|
M****7
发帖数: 214 | 16 GQ Daley 2010 "Epigenetic memory in induced pluripotent
stem cells"
【在 m******5 的大作中提到】
: Lz is thinking in the absolutely right direction.... too bad there are at
: least 2 nature cell
: biology paper published in this topic
|
M****7
发帖数: 214 | 17 GQ Daley 2010 "Epigenetic memory in induced pluripotent
stem cells"
【在 m******5 的大作中提到】
: Lz is thinking in the absolutely right direction.... too bad there are at
: least 2 nature cell
: biology paper published in this topic
|
M****7
发帖数: 214 | 18 GQ Daley 2010 "Epigenetic memory in induced pluripotent
stem cells"
【在 m******5 的大作中提到】
: Lz is thinking in the absolutely right direction.... too bad there are at
: least 2 nature cell
: biology paper published in this topic
|
n********k
发帖数: 2818 | 19 there was too come out the same time I believe...both from harvard? and
there was tons of news too, i don't know for what? the same old stories on
news again, or what? many experts were praising its a big deal too but do
they really buy that? they know better than that at least from the nuclear
cloning lessons...
【在 M****7 的大作中提到】
: GQ Daley 2010 "Epigenetic memory in induced pluripotent
: stem cells"
|
m******5
发帖数: 1383 | 20 yes! that is what i am talking about
【在 M****7 的大作中提到】
: GQ Daley 2010 "Epigenetic memory in induced pluripotent
: stem cells"
|
