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Biology版 - Which cell type for cell transformation assay is the best?
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话题: cell话题: assay话题: type话题: best
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1 (共1页)
q**********0
发帖数: 335
1
To evaluate a viral protein's cacinogenesis ability, we are using cell
transformation assay. which cell line is the best choice to do this type of
assay? Thanks.
j****x
发帖数: 1704
2
我们用NIH-3T3在nude mice上做in vivo assay。in vitro自然也可以
很难说有best choice,和你蛋白的种类和来源以及下游设计都有关系

of

【在 q**********0 的大作中提到】
: To evaluate a viral protein's cacinogenesis ability, we are using cell
: transformation assay. which cell line is the best choice to do this type of
: assay? Thanks.

q**********0
发帖数: 335
3
Thanks for the reply. I hear NIH-3T3 has high background, and also NIH-3T3
is immortalized, then how can it will be transformed? Thanks.

【在 j****x 的大作中提到】
: 我们用NIH-3T3在nude mice上做in vivo assay。in vitro自然也可以
: 很难说有best choice,和你蛋白的种类和来源以及下游设计都有关系
:
: of

1 (共1页)
进入Biology版参与讨论
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话题: cell话题: assay话题: type话题: best