d***y
发帖数: 8536 | 1 最近在構建一個非常狗血的質粒，想試試gibson assembly。看官們對這個方法有啥評
價？ 成功率高嗎？ |
h**********r
发帖数: 671 | |
s******s
发帖数: 13035 | 3 实验室有人做过，说很方便容易，多数clone都是对的。我这个礼拜gblock到了就会做
，会告诉你结果
【在 d***y 的大作中提到】
: 最近在構建一個非常狗血的質粒，想試試gibson assembly。看官們對這個方法有啥評
: 價？ 成功率高嗎？
|
x********u
发帖数: 430 | 4 Follow the NEB Gibson assembly protocol. It's pretty cool.
You might also want to try Red/ET recombination provided by Gene Bridge. |
h**********r
发帖数: 671 | 5 Perhaps we will get Gibson assembly kit from NEB. Thank you so much for your
info! |
g*********5
发帖数: 2533 | 6 Red/ET need 50nt and Gibson 15bp nt...
Red/ET is good for mice target vectors?
【在 x********u 的大作中提到】
: Follow the NEB Gibson assembly protocol. It's pretty cool.
: You might also want to try Red/ET recombination provided by Gene Bridge.
|
h***e
发帖数: 146 | |
