由买买提看人间百态

boards

本页内容为未名空间相应帖子的节选和存档,一周内的贴子最多显示50字,超过一周显示500字 访问原贴
Biology版 - 如果His tagged protein is present in the soluble fraction, 是否可以直接做kinetic assay?
相关主题
Re: About protein size, any suggestion?做了cell fractionation以后, nucleus里面啥都没了。。。
请问酶动力学是干什么的?NGS/Array Job opportunity -Illumina
Job opportunity in Shanghai:Research Assistant / Associate - BiochemistryTemp position:RA in Genentech (转载)
referral help求推荐:96 well plate 哪家公司的比较好?
关于nuclear loading control的问题Position Available: Associate Scientist, High Throughput Screening/Assay Development
求subcellular fractionation 的protocol或kit推荐Re: protein的溶解性?
Histone H3可以做nuclear fraction positive marker吗?Re: 谁有经验? 关于dimerization 和 native gel
求推荐Nucleo-cytoplasmic fractionation的kitchaperone表达位置
相关话题的讨论汇总
话题: his话题: soluble话题: fraction话题: kinetic话题: tagged
进入Biology版参与讨论
1 (共1页)
j*****a
发帖数: 92
1
如果His tagged protein is present in the soluble fraction是否可以直接做kinetic assay?
n***w
发帖数: 2405
2
no.
after your extraction, if most fusion protein is present in the soluble
fraction instead of solubilized pellet, then you can assume there may not be
inclusion bodies.
j*****a
发帖数: 92
3
His tagged protein is present in the soluble fraction, 是否可以直接做kinetic
assay?
o*****r
发帖数: 156
4
most likely yes

kinetic

【在 j*****a 的大作中提到】
: His tagged protein is present in the soluble fraction, 是否可以直接做kinetic
: assay?

1 (共1页)
进入Biology版参与讨论
相关主题
chaperone表达位置关于nuclear loading control的问题
从水滴到彩虹——2010年神经所年会蒲慕明的讲话ZT求subcellular fractionation 的protocol或kit推荐
问蛋白降解是不是本身的性质决定的?Histone H3可以做nuclear fraction positive marker吗?
用什么样的乙醇配制细胞刺激剂求推荐Nucleo-cytoplasmic fractionation的kit
Re: About protein size, any suggestion?做了cell fractionation以后, nucleus里面啥都没了。。。
请问酶动力学是干什么的?NGS/Array Job opportunity -Illumina
Job opportunity in Shanghai:Research Assistant / Associate - BiochemistryTemp position:RA in Genentech (转载)
referral help求推荐:96 well plate 哪家公司的比较好?
相关话题的讨论汇总
话题: his话题: soluble话题: fraction话题: kinetic话题: tagged