H*****e
发帖数: 120 | 1 Hi,
I have some samples that I prepared for microarray. When I went to the core
, I noticed that the core has only a few people do array now but most of
them do RNA-seq. They pass me some information like I know everything. But
, after read them for the whole morning, I still no idea what to do.
Especially,
1. Library: What is the difference and advantage between Single Read and
Paired end and mRNA and small RNA libraries.
2. Lane: some run one lane and some run 1/2 lane.
3. Cycle numbers: h | d***y
发帖数: 8536 | 2 1 Paired end is much better than single end.
2 Normally,1 lane is necessary. About 1/2 lane, I think they mix your sample
with other sample in the one lane?
3 I don't know the cycle numbers. You could call customer service of the
sequence company.
Good luck. |
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