s********l
发帖数: 1195 | 1 Hi, I'm pretty new to the field of immunoflurescence.
I need to order an antibody specific for a nuclear protein. The antibody
used was rarely published and only one paper cited that monoclonal antibody
302-18 and 302-22 are specific to this protein. I need to order the mouse
type and use FITC as the second antibody for furescent labeling.
Is it enough to only provide this much info to the antibody provider
companies and to place an order? Anybody please provide some suggestions.
Thanks in advan |
s******y
发帖数: 28562 | 2 You usually need the catalog number of that product, or at least need to
know the clonal number of the antibody.
You can call the company and they will explain to you.
Remember it is their job to be patient with you.
antibody
【在 s********l 的大作中提到】
: Hi, I'm pretty new to the field of immunoflurescence.
: I need to order an antibody specific for a nuclear protein. The antibody
: used was rarely published and only one paper cited that monoclonal antibody
: 302-18 and 302-22 are specific to this protein. I need to order the mouse
: type and use FITC as the second antibody for furescent labeling.
: Is it enough to only provide this much info to the antibody provider
: companies and to place an order? Anybody please provide some suggestions.
: Thanks in advan
|
s********l
发帖数: 1195 | 3 Thanks for the reply.
Do you think the 302-18 and 302-22 I mentioned above are the "clonal number"
?
【在 s******y 的大作中提到】
: You usually need the catalog number of that product, or at least need to
: know the clonal number of the antibody.
: You can call the company and they will explain to you.
: Remember it is their job to be patient with you.
:
: antibody
|
h*****o
发帖数: 342 | 4 what protein is it?
number"
【在 s********l 的大作中提到】
: Thanks for the reply.
: Do you think the 302-18 and 302-22 I mentioned above are the "clonal number"
: ?
|
s********l
发帖数: 1195 | 5 NMP22 (nuclear matrix protein 22)
A company invented a kit like a "pregnancy test" for it, not sure if the
company patented the immunoassay contents, it's weird that no publications
mentioned the exact biological name for the antibody.
Any suggestions?
【在 h*****o 的大作中提到】
: what protein is it?
:
: number"
|
s******y
发帖数: 28562 | 6 Just call the company and ask the technical support.
【在 s********l 的大作中提到】
: NMP22 (nuclear matrix protein 22)
: A company invented a kit like a "pregnancy test" for it, not sure if the
: company patented the immunoassay contents, it's weird that no publications
: mentioned the exact biological name for the antibody.
: Any suggestions?
|
