c******d
发帖数: 306 | 1 我有50只rats，每只有四个tissue的样品。RNA测序，有multiplex，每个channel该怎
样分配样品？是一个channel放没只老鼠的四个tissue，还是把所有老鼠的同一个
tissue放在同一个channel。Illumina的方法，一个channel最多可以有多少个样品？ |
c******d
发帖数: 306 | 2 No one has such experience? help please. |
T**********t
发帖数: 1604 | 3 Why not ask Illumina's tech support... |
c******d
发帖数: 306 | 4 one more question. Can the sequencing results be used for QTL mapping? we
could know the SNPs then the genotype of certain area of chromosome. it
could be used for mapping. the only issue should be all the snps come from
coding region. |
a****o
发帖数: 1786 | 5 if you want to compare different tissues, you should put tissues from the
same mouse in one channel; if you want to compare individuals, you should
put the same tissue in one channel.
you can normalize to the signal to total reads later. I guess it won't make
a big difference. We routinely barcode 12 samples. It can be more.
【在 c******d 的大作中提到】
: 我有50只rats，每只有四个tissue的样品。RNA测序，有multiplex，每个channel该怎
: 样分配样品？是一个channel放没只老鼠的四个tissue，还是把所有老鼠的同一个
: tissue放在同一个channel。Illumina的方法，一个channel最多可以有多少个样品？
|
c******d
发帖数: 306 | 6 did ur use mRNA seq for gene expression investigation? have u ever try to
use SNP also. seems it is biased, because all SNPs come from coding region.
make
【在 a****o 的大作中提到】
: if you want to compare different tissues, you should put tissues from the
: same mouse in one channel; if you want to compare individuals, you should
: put the same tissue in one channel.
: you can normalize to the signal to total reads later. I guess it won't make
: a big difference. We routinely barcode 12 samples. It can be more.
|
