z*****n
发帖数: 236 | 1 MTT Assas Solution放在37度水浴两天,还能用吗? 有什么补救措施,万分感谢! 急,在
线等!来不及试了,老板极品! |
f*********r
发帖数: 1233 | 2 哪一瓶？我觉得没事。这东西不是抗体之类的活性蛋白。基本上算化学试剂。这两种
buffer本身工作温度就是37度。
你要没把握，可以找个多余的细胞培养盘试试。 |
z*****n
发帖数: 236 | 3 Thank you very much. I am in the lab, can't input Chinese. I accidentally
forgot to take the dye solution bottle (not the solubilization/stop solution
) from the 37C water bath and it stayed there for 40 hours. Tomorrow, I will
run the experiment. I don't think I have time to test it before that. I
worried so much about my JIPIN boss coz she will definitely get mad at me.
Yes, it is an organic chemical solution ( the active component is called
tetrazole), not protein thing. Hopefully, it is fine
【在 f*********r 的大作中提到】
: 哪一瓶？我觉得没事。这东西不是抗体之类的活性蛋白。基本上算化学试剂。这两种
: buffer本身工作温度就是37度。
: 你要没把握，可以找个多余的细胞培养盘试试。
|
f*********r
发帖数: 1233 | 4 今天下午，随便找一盘多余的细胞，把这东西放进去，37度孵育。到下班之前就能看得
出来它是不是跟正常一样。不用等那个solubilization溶液。
我还是相信，它不会有问题。 |
z*****n
发帖数: 236 | 5 Thanks a million. I will give you 50 Baozi if it is fine after I test it
either today or tomorrow. Just to thank you! |
f*********r
发帖数: 1233 | 6 包子是干什么用的？
【在 z*****n 的大作中提到】
: Thanks a million. I will give you 50 Baozi if it is fine after I test it
: either today or tomorrow. Just to thank you!
|
z*****n
发帖数: 236 | 7 包子是吃的，哈哈！
今天做了MTT Assay，试验显示这个试剂还是挺耐热的，呵呵，而且结果还不错，极品
老板都TMD笑了。非常非常感谢你的答复，让我感觉好多了。50个包子（伪币）已经给
你转过去了，请查收！
再次感谢！ |
