c*t
发帖数: 1063 | 1 怎么确定Genotype-phenotype correlation？
显示特定phenotype的knockout mice 在换了background之后却失去了phenotype，是不
是能够证明原来的推断不是那么可靠？
如果再换一个别的background还是没有phenotype呢？能说明什么？
欢迎前辈解答.
谢谢!! |
a***e
发帖数: 1010 | 2 If knocking-out of gene A induces a LOF phenotype in a certain mouse
strain
and adding back A rescues that LOF phenotype, we argue that gene A is
involved in generating this phenotype.
However, if there are no LOF phenotype when gene A is similarly knocked
out
in other mice strains, it is very possible that there are additional
molecular events involved in this phenotype generation.
|
c*t
发帖数: 1063 | 3 Thanks for the reply.
How to rescue the LOF phenotype in the knockout mice? |
a***e
发帖数: 1010 | 4 you can create a transgene that overexpresses the gene A which you are
interested, integrate this transgene into your konck-out mice by
conventional techniques, and examine whether this transgene rescues the
LOF phenotype.
BTW, nearly all of the publications don't have this rescue assay, hinting rescue is an unnecessary experiment.
【在 c*t 的大作中提到】
: Thanks for the reply.
: How to rescue the LOF phenotype in the knockout mice?
|
D*a
发帖数: 6830 | 5 貌似有不少的knockout mice 都有strain特异性的。。。
具体例子不知。。。
对了，好像agouti这个基因就跟obesity有点儿关系。。。 |
l**********n
发帖数: 201 | 6 there are some literatures using the strategy you described to solidify the
claim of geno-pheno correlation. it's not so usual in mouse genetics field
simply because of technical challenging - you need years to generate a KO
line, and another year or so to generate transgene, another year to breed
mice ... so reviewers usually would not demand this kind of rescue exp.
rescue is an unnecessary experiment.
【在 a***e 的大作中提到】
: you can create a transgene that overexpresses the gene A which you are
: interested, integrate this transgene into your konck-out mice by
: conventional techniques, and examine whether this transgene rescues the
: LOF phenotype.
: BTW, nearly all of the publications don't have this rescue assay, hinting rescue is an unnecessary experiment.
|
a***e
发帖数: 1010 | 7 An alternative way examining the genotype-phenotype relationship is to test
whether several independent animal strains harbor different mutations in the
same gene and elicit similar phenotypes. |
