如题，大家是如何选择二抗的荧光抗体的？ 只为确定两蛋白的共定位。
因为 Red TEXAS 染料在荧光显微镜下很弱，难以检测到。
s******y 发帖数: 28562
2
The principle is to choose dyes with emission spectra that are separated enough without major overlap.
I believe the spectra of FITC and TRITC are not overlapped.
You should check here: http://www.mcb.arizona.edu/IPC/spectra_page.htm
I think your problem is probably due to:
1. the unspecific binding of your 2nd ab
2. one dye has too strong a signal and leak into the other detection channel
