h********r
发帖数: 12 | 1 I transferred my HA-tag myr-AKT plasmid into my cells and conducted the
selection of stable cells. After selection, the selected clonies exhibited
AKT expression through the examintion of HA-tag antibodies in Western blot.
Moreover,the fast tumor formation was observed in nude mice implanted cells
expressing myr-AKT. However, currently, when I used these cells (several
passages cell culture) for examining phospho-AKT, upstream signals or
downstream signals such as phospho-GSK,phospho-S6K and som | m**********3
发帖数: 706 | 2 "However, currently, when I used these cells (several
passages cell culture) for examining phospho-AKT, upstream signals or
downstream signals such as phospho-GSK,phospho-S6K and some others in
western blot, it is difficult to obtain the signal from western blot"
Do you mean you can not get any signal for all these antibodies? Or there is
no difference between your stable cell line and the parental cell line? | h********r
发帖数: 12 | 3
is
I can not get any signal for all these antibodies. Could you please tell me
the reasons? Thanks!
【在 m**********3 的大作中提到】
: "However, currently, when I used these cells (several
: passages cell culture) for examining phospho-AKT, upstream signals or
: downstream signals such as phospho-GSK,phospho-S6K and some others in
: western blot, it is difficult to obtain the signal from western blot"
: Do you mean you can not get any signal for all these antibodies? Or there is
: no difference between your stable cell line and the parental cell line?
| m*****y
发帖数: 857 | 4 maybe the pathway is not activated in your system?
.
cells
Could
【在 h********r 的大作中提到】
: I transferred my HA-tag myr-AKT plasmid into my cells and conducted the
: selection of stable cells. After selection, the selected clonies exhibited
: AKT expression through the examintion of HA-tag antibodies in Western blot.
: Moreover,the fast tumor formation was observed in nude mice implanted cells
: expressing myr-AKT. However, currently, when I used these cells (several
: passages cell culture) for examining phospho-AKT, upstream signals or
: downstream signals such as phospho-GSK,phospho-S6K and som
| n****e
发帖数: 1677 | 5 can you see akt expression?
.
cells
Could
【在 h********r 的大作中提到】
: I transferred my HA-tag myr-AKT plasmid into my cells and conducted the
: selection of stable cells. After selection, the selected clonies exhibited
: AKT expression through the examintion of HA-tag antibodies in Western blot.
: Moreover,the fast tumor formation was observed in nude mice implanted cells
: expressing myr-AKT. However, currently, when I used these cells (several
: passages cell culture) for examining phospho-AKT, upstream signals or
: downstream signals such as phospho-GSK,phospho-S6K and som
|
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