Biology版 - Re: Apoptosis---Re: Call for MCBJC dicus |
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M****e
发帖数: 70 | 1 i have just read the paper and found it is more interesting than i have
expected. they basically took a chemical screening/biochemical analysis
approach to identify novel genes that regulate the activation of caspase-3.
they found a chemical compound, namely PETCM, that could promote apoptosis,
then they fractionated protein extracts and looked for proteins that mediated
the apoptome promotion effect of PETCM; and they characterized several PHAP
genes that are tumor suppressors as well as ProT w | l*****k
发帖数: 587 | 2 It is an amazing paper, proof of principle of HTS methods for drug screening
and deciphering
complicated signal transduction network.
However, I don't get it when they say: "In contrast, the presense of PHAPI did
not affect ... and
increased caspase-9 was associated with apoptosome" This should be the natural
function of PHAPI, right? and did they do a timecourse? as to if add PHAPI
first, then
ProT later, will this be reversed? I probably missed something here.
and why they only tested PHAP1, I
【在 M****e 的大作中提到】
: i have just read the paper and found it is more interesting than i have
: expected. they basically took a chemical screening/biochemical analysis
: approach to identify novel genes that regulate the activation of caspase-3.
: they found a chemical compound, namely PETCM, that could promote apoptosis,
: then they fractionated protein extracts and looked for proteins that mediated
: the apoptome promotion effect of PETCM; and they characterized several PHAP
: genes that are tumor suppressors as well as ProT w
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| 相关主题 |
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| ● Re: MCBJC, cell division (discussion?) | ● Interesting stuff | | ● MCBJC, cell division, discussion | ● 用Cleaved caspase-3抗体做IF-P | | ● Re: MCBJC, cell division (more discussion, 我又来乐) | ● 学术问题，apoptosis or DNA damage? | | ● paper help/ call for MCBJC discussion | ● 问个学术问题，有什么peptide在ER里聚集会导致apoptosis | | ● Re: denatured protein是不是还有聚集体? | ● 请教做apoptosis的大牛 | | ● Re: MCBJC, cell division, Jan 20 2003, by Assasin | ● 做过apoptosis 和 necrosis 的同学请进 | | ● Re: biological word translation | ● 细胞凋亡研究疑问 | | ● 问一下ZVAD－FMK，请高手帮忙，多谢了。 | ● no apoptosis,no cell necrosis, has cell autophage but inhibitor can't enhance cell death |
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