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Biology版 - Re: What method do you use for DNA transfection?
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请教SF9 细胞的transfection方法求助Hela transfection
求助: transfection reagent3T3 transfection效率太低
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用什么办法能提高fibroblast 和MEF转染效率呢,question about cotransfect plasmid and siRNA
Transfection reagent for SH-SY5Y ?推荐一个转sirna的transfection reagent吧。
向大家请教transfection的问题请教siRNA转染
Arguing with Boss with co-transfectionSiRNA transfection 可以买dharmacon的smartpool直接用lipofectamine转进动物细胞吗
请教Dual Luciferase Assaymacrophagy细胞transfection求助
相关话题的讨论汇总
话题: what话题: dna话题: transfect话题: use
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1 (共1页)
R*s
发帖数: 2041
1
yes, immortalized cell lines are generally harder to transfect
than primary cells.
however, 283 in general should be easy to transfect unless your
genes are really toxic to the cells (some of genes I have to use
are in such case).
Some guy upstair mentioned Superfect Reagent from Qiagen.
That one is quite effective, and better than LipofectAmine
in many cases, but more expensive too.
1 (共1页)
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macrophagy细胞transfection求助Transfection reagent for SH-SY5Y ?
快疯了~~Luciferase assay向大家请教transfection的问题
有人transfect过primary beta cell吗?不用virus?Arguing with Boss with co-transfection
请教,FRET的两个质粒共转染不能同时表达在一个细胞内请教Dual Luciferase Assay
请教SF9 细胞的transfection方法求助Hela transfection
求助: transfection reagent3T3 transfection效率太低
MEF 细胞转染如何transfect siRNA into MEFs
用什么办法能提高fibroblast 和MEF转染效率呢,question about cotransfect plasmid and siRNA
相关话题的讨论汇总
话题: what话题: dna话题: transfect话题: use