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发帖数: 27 | 1 yeap, man, it is like that you first have to make a DNA
competitor that can be amplified with the same primer
sets. however, the size of the product is different than
the RTPCR product, normally it is 30-100bp less than
that. so it is an internal deletion of the RTPCR product.
then you add different amount of the competitor to the
RT product, and do PCR. run the gel through a machine,
sort of like HPLC. there should be three major products,
the cDNA, the truncated cDNA, as well as a hybrid of
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