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全部话题 - 话题: screenings
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d********g
发帖数: 10550
1
来自主题: Linux版 - 有人觉得screen不好用吗
tmux至少还在维护,screen没啥动静。tmux有个sample config是模仿screen的快捷键
,我一般就用那个。说起来这个sample config当年还有typo,是我去给fix的
w***g
发帖数: 5958
2
来自主题: Linux版 - 有人觉得screen不好用吗
我觉得screen没有不好到要换成tmux. 几个基本功能足够用了.

screen
d******0
发帖数: 3640
3
有没有用OpenSuse的大虾?我在KDE桌面下,点开始
菜单(蜥蜴头),选择电源按钮,里面有log out,
lock screen,power off 等等,一旦选择了lock screen,
这机器就完旦了,症状就是等logout之后,再想login进
入图形desktop, 机器慢得无法使用,几十分钟才能完
成login过程,然后点一下鼠标需要十几分钟才见反映,
非得重装操作系统。这问题我从10.1到13.2都中过招儿,重
装过不下5次。有没有人遇到过类似问题,可知道解决办法?
多谢!
C***N
发帖数: 200
4
给朋友一个brand new netbook作为圣诞礼物,但是他打开后不work。请教高手,是什
么原因,怎么处理?
下面是故障描述:
WHEN YOU TURN ON THE COMPUTER THE START UP SCREEN IS FROZEN AND YOU CANNOT
DO ANYTHING. IT'S LIKE ALL THE ENTIRE SYSYEM IS FROZEN WE HAVE TRIED TURNING
ON AND OFF SEVERAL TIMES ITS LIKE IT WONT BOOT, IT WON'T TO ANYTHING BUT A
SCREEN THAT WON'T DO ANYTHING
b****p
发帖数: 3293
5
【 以下文字转载自 Windows 讨论区 】
发信人: bravep (勇敢的心), 信区: Windows
标 题: Windows XP Home did not boot,got Blue screen
发信站: BBS 未名空间站 (Wed Nov 21 11:38:35 2007)
I saw a blue screen said that "a problem has been detected and windows has
been shut down to prevent damage to your computer" and so on and
"technical information:
stop: 0x0000008E(0X00000000s 0xBF84CAA6, 0XF7F09C30 0X00000000)
Win 32K.sys --address BD84CAA6 base at BF800000, Datestamp 45013F6
Then I started the PC with installation CD and used the
d*******r
发帖数: 249
6
I posted this on hardware too. First sorry for no chinese input on this
computer.
Here is what happened today:
My laptop turned to black screen when it was running and I shut it off.
Then I tried to restart it, a black screen showed up with the power LED and
memory light on and the fan running but with no other signs of activity.
tried multiple times......still the same problem. What's wrong with it and
what should I do? The warranty is expired already. How much it will cost
if I take it to a l
n*w
发帖数: 3393
7
来自主题: Software版 - Re: Screen+Emacs真是强大呀 (转载)
【 以下文字转载自 Linux 讨论区 】
发信人: nkw (非死非活), 信区: Linux
标 题: Re: Screen+Emacs真是强大呀
发信站: BBS 未名空间站 (Thu Oct 9 21:15:10 2008)
借个人气问一下,cygwin里在screen里用vi非常的暗淡无光,肉眼很难看清。有什么办
法不?
n*w
发帖数: 3393
8
来自主题: Software版 - Re: Screen+Emacs真是强大呀 (转载)
真是不明白为什么vim在cygwin的screen里就变暗,实在没道理。为此我不得不放弃
screen
C***N
发帖数: 200
9
给朋友一个brand new netbook作为圣诞礼物,但是他打开后不work。请教高手,是什
么原因,怎么处理?
下面是故障描述:
WHEN YOU TURN ON THE COMPUTER THE START UP SCREEN IS FROZEN AND YOU CANNOT
DO ANYTHING. IT'S LIKE ALL THE ENTIRE SYSYEM IS FROZEN WE HAVE TRIED TURNING
ON AND OFF SEVERAL TIMES ITS LIKE IT WONT BOOT, IT WON'T TO ANYTHING BUT A
SCREEN THAT WON'T DO ANYTHING
h***o
发帖数: 539
10
run screen
ctrl-a c: create a new window
ctrl-a number: go the window number
ctrl-a d: detach
run screen -x, get them back
我现在也就知道这么多
s*****o
发帖数: 17
11
Every thing was fine before I change a new monitor. After that, no matter I
open an software, when I maximum it, the application always go out of the
screen edge. I have to move it back a little bit then I can see the "Maximum
", the "Minimize" and the "close" button in the top right corn.
It is not very convenient. Does anybody have any idea how I can make the "
Maximum" button exactly fitting the edge of screen. Thanks.
Sorry I can not input Chinese now.
h*********0
发帖数: 18
12
Surface Pro 2,
电视用的是roku,
本来win 8,很容易就通过roku project to second screen.
升级win 10以后,就无法了。点击"duplicate screen "了,没有反应。
有谁遇到过这个情况吗?
p*****r
发帖数: 64
13
来自主题: Biology版 - 请教drug screen中的技术问题
谢谢上面几位的建议. 我也试过用paper towels,但384板上不是每个孔都平均的去掉
medium,所以不行.而且每次靠手来控制力度的话,差异更大. ABI 基本上没什么建议.
如果加一个板子的话,靠人还行,要是加100个板子,根本不可能做到.
做high-throughput screen, 我现在的确是看delta-delta C(t) right, 理论上不担心
每个孔出来到值,但是在284板子上,要用32个positive control 和32个DMSO 做Z
fanctor分析.这样的话,positive control 和DMSO组内的差异必须很小才行.所以只有
组内每个孔细胞数基本一直才行.
还有就是用qPCR 做screen的确耗钱,但现在是花了钱,也不能保证稳定. 真是麻烦阿.
s********n
发帖数: 2939
14
来自主题: Biology版 - 关于Biometrics screening.
事情是这样的: 下一年公司的保险要换,给了一些discount(数额还不小),但是要求
做Biometrics screening和Health check,如果不做的话就没有Discount,但不影响
plan。
我查了一下,biometrics screening好像是检查遗传信息(不确定,比如癌症和糖尿病
等)。我的担心是一旦做了这样的检查以后,是不是保险公司能够根据情况调整保费或
者拒保?而且这样的信息一旦有了,估计一生都是如此了,是不是很危险?
BTW:好像美国是全球最大的biometrics information provider。
d****u
发帖数: 1553
15
来自主题: Biology版 - 问个关于RNAi screen的问题
谢谢回复,你说的可能是针对单一基因的knockdown而不是针对screen的。做基因组级
别的pooled RNAi screen的时候,在筛选结束要抽基因组DNA。然后PCR扩增hairpin
sequence。再去上array或sequencing,就可以知道哪些hairpin富集了,哪些hairpin
减少了。 我问的是这一步,这时候因为所有的不同基因的hairpin都是混在一起的,所
以不可能得到特定基因的knock down水平
b*****l
发帖数: 9499
16
正好前些日子读到去年的一篇 Gupta/Weinberg/Lander 合作的 Cell 文章,讲如何 ta
rgetting EMT 来 HT screening selective inhibitors against cancer stem cell 的
。既然是 targetting EMT 么,大约也适合你的 metastasis-specific inhibitor 的要
求。
Gupta, P. B.; Onder, T. T.; Jiang, G.; Tao, K.; Kuperwasser, C.; Weinberg, R
. A. & Lander, E. S. Identification of selective inhibitors of cancer stem c
ells by high-throughput screening. Cell, oadinstitute.org, 2009, 138, 645-65
9
T****i
发帖数: 15191
17
来自主题: Biology版 - 好的和差的genetic screen
看起来很象,但有本质不同。
很笨的brute force genetic screen 结果是很不确定的,是发散的。你不知道会得到
什么基因。这就是genetic screen 的魅力。你得到了基因,才是开始。
而我说的试错法,是一个一个试你“认为”的可能性,在思维上是收敛的。很可能你认
为的可能性根本就不包括真正的机制。所以随着时间,你会不断补充新的可能性。
我再次说一下,没有绝对的不试错。我也试错。但一个明白人,不会去试所有的可能,
而是会prioritize。
会不会prioritize,会不会分辨,决定你是不是试错法。
T*****n
发帖数: 897
18
【 以下文字转载自 Postdoc 讨论区 】
发信人: Tonyvan (Tony), 信区: Postdoc
标 题: Drug discovery 的high throughput screening data怎么分析呢?-诚心求教!!!
发信站: BBS 未名空间站 (Tue Nov 5 20:19:07 2013, 美东)
问一下,Drug discovery 的high throughput screening data怎么分析呢?刚刚回到
一个做这方面的领域,感觉挺喜欢的,但自己没这方面的经验,不知道怎么分析。老板
只是打击我,不肯指导我。现在很郁闷,诚心求教!!!
V******t
发帖数: 444
19
同求问。我正在做screen,原来是用Haploid 细胞+piggyback 做forward genetic
screen
现在也打算用CRISPR。求科普+八卦
d****u
发帖数: 1553
20
哺乳动物细胞里做genome wide crisper screen的就那么几篇吧,都在大牛杂志上呢。
至于Cas9和sgRNA是不是在一个载体上,根据我们的经验,目前最好用的是Zhang Feng
lab的version 2 all in one vector。
有几个实验室载体分开的,必然会有某一个载体的滴度特别低。过这个情况也因为细胞
的不同而异,我们用的是原代细胞,不见得有普遍性。
不过我完全不能理解为什么CRISPR要分成两个载体然后再搞一个inducible system。
从screen的角度来讲,crispr library的数据明显要比shRNA library的数据信噪比高.
V******t
发帖数: 444
21
我刚收到addgene上买来的feng zhang的library
以后基于细胞的screen就是个常规实验了。很多基因很快就要被一网打尽了
要是这些screen都能自动化就好了 可惜没人愿意把生物实验室自动化、标准化啊
f******g
发帖数: 1003
22
想做个small molecule screening,但是自己又不是这个方面出身的,请问国内有做
small molecule screening 比较好的公司吗?
费用如何?
thanks
d****u
发帖数: 1553
23
来自主题: Biology版 - shRNA/crsipr screen后deep sequencing
看你的library有多大,通常reads number保证你screen的representation就好。如果
是genome wide screen的话miseq可能不够。
T*****n
发帖数: 897
24
【 以下文字转载自 Postdoc 讨论区 】
发信人: Tonyvan (Tony), 信区: Postdoc
标 题: Drug discovery 的high throughput screening data怎么分析呢?-诚心求教!!!
发信站: BBS 未名空间站 (Tue Nov 5 20:19:07 2013, 美东)
问一下,Drug discovery 的high throughput screening data怎么分析呢?刚刚回到
一个做这方面的领域,感觉挺喜欢的,但自己没这方面的经验,不知道怎么分析。老板
只是打击我,不肯指导我。现在很郁闷,诚心求教!!!
A******y
发帖数: 2041
25
Imatinib is from high throughput screening library. However, if you ask Dr.
Druker, he will tell you it is rationally designed, muhahaha. Yea, it is a
semi-screening effort and a lot of luck.
Btw, if you are in academia and try to write a HTPS grant, good luck to you.
This also apply to other efforts such as combi-chem and computational etc.
And if you are not in the academia, you better hope your collection is
private and is not in the public domain or element of composition patent is
har... 阅读全帖
n***8
发帖数: 634
26
来自主题: MedicalCareer版 - UW screen capture?
I tried to capture screen of UW, but failed.
I believe they have something to prevent you from capture screen.
J******i
发帖数: 1
27
来自主题: MedicalCareer版 - Questions about VISA and TB screening
Hi all,
There was a saying that much more residency programs are willing to provide
J1 instead of H1B visa. Is it true? I won't be able to obtain a green card
before Match; therefore, I definitely need a J1 or H1B. If the options left
were programs only providing J1 visa, am I supposed to get a job in a
Chinese hospital beforehand? Since I also heard that you want to have a base
in your citizenship country so that the program could apply to the US
department of immigrants for a ds-2019 for you.
... 阅读全帖
K********S
发帖数: 14
28
有从国内过来的护士考过RN,办visa screen要寄回国内盖章么?
请问这里有从国内F2,H4过来,在国内是护士,然后在这里考过RN,办visa screen时还
要寄回国内盖章么?
可是国内很多地方都不给盖章,北京是不行,有什么好办法么?
thanks!
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