w*****4
发帖数: 2 | 1 1099R box 1 gross distribution = RRB-1099R box 7 total gross paid
1099R box 5 employee contribution = RRB-1099R box 3 employee contribution |
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H*****e
发帖数: 120 | 2 Hi experts:
I want to explore epigenome direction with some fund left in my budget.
After talking with BGI about epigenome sequencing, I prefer to start with
DNA methylation. CHIP would be too technique for us as a fresh hand. BGI
provides a low cost one called RRBS. The price is very attractive. However,
I worry for the future publication and grant application using these data.
In practice, RRBS is what we can handle because we are not able to handle
the gene desert. On paper, we try to cover... 阅读全帖 |
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H*****e
发帖数: 120 | 3 Sorry, I did not explain myself clear. I guess that my mind is puzzled with
too many question and try to get answer once.
I will ask BGI to do it. Since it is too expensive, with available fund, I
can only have two imperfect choices:
1. to combine samples: for example, combine all controls and all tumors. Run
two whole genome bis-sequence.
2. down to RRBS or eRRBS, to do 3 controls and 3-5 tumors.
The first one lose the statistical power; we may have trouble to publish it
in the future. Howeve... 阅读全帖 |
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m*********o
发帖数: 7545 | 4 它的BOX 3 是EMPLOYEE CONTRIBUTION. BOX 4 是 CONTRIBUTORY PAID
那1099-R 常规 FORM BOX 1 GROSS DISTRIBUTION 是不是 就是 RRB BOX 1 + BOX 4 ? |
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A******u
发帖数: 61 | 5 用eRRBS啊，增强型RRBS 方法见Stadler et al., 2011
我们的实际操作中
70％ reads能map回reference genome
能覆盖90% CGI (10X seq)
40% gene promoter
20-60% LMR／UMR
我的理解，弱点自然是如果DNA 甲基化的变化没在这些区域，你就看不到咯。
However,
. |
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H*****e
发帖数: 120 | 6 Thanks so much!
I had spent the whole afternoon reading this paper. It is very interesting
and encouraging. However, one question puzzling me is that how confident we
can say non-BBRS covered area is not as important. My worry is that, if it
is necessary, we can combine sample to d whole-genome. But in that way, we
lose the individual variation/statistical analysis power. However, if we
can spent same money to do RRBS and not much question, we could do more
samples.
When I was student, my P... 阅读全帖 |
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x*********n
发帖数: 28013 | 7 谢谢大猫猫refer。
直白的问题就一个：
IBGP VS eBGP, why we need full mesh on iBGP
剩下的都是情景题。
RR, confederation,
at confederation, if sub-AS pass to another, sub-AS, will BGP attribute pass
or not?这个要我回答是or不是，我不太确定，就说sub AS的information应该是独立
的，不应该传给别人，他问我为啥这么想，我说传给别人了是不是要loop了啊?当时好
紧张，好混乱，寒风习习的，心那个凉啊。
router reflector, 2 group, 3 client, how packet goes from client1 to RR,
then RRA to RRB, then ....then what would happen, ( drop) what RRA do when
RRB send back, (Cluster-ID)基本上就是要我一步步的往哪里走，只有前面2步我貌似
走对了，他说correct，后... 阅读全帖 |
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g*s
发帖数: 2277 | 8 http://www.irs.gov/publications/p505/ch03.html
Reporting your withholding. Report on your tax return all federal income
tax withholding shown in box 4 of a Form 1099, box 6 of Form SSA-1099, and
box 10 of Form RRB-1099. Include the amount withheld in the total on line 62
of Form 1040, line 36 of Form 1040A, or line 7 of Form 1040EZ. |
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g**********8
发帖数: 273 | 9 在Form 1040表中，line 20a, Social security benefits 可以从W-2中计算出来吗？
另外，我为什么没有收到ssa-1099 和 rrb-1099 ?
多谢。 |
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d****u
发帖数: 1373 | 10 SOA annual meeting的presidential luncheon上面攻击抹黑中国、私下session上发传
单。
Isaiah L Forrest
Senior Actuary
US Railroad Retirement Board
844 North Rush Street
Chicago, IL 60611
United States
Tel: (312)751-4917
Fax: NA
Email: f*****[email protected] |
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l**********1
发帖数: 5204 | 11 not only wet apporach
also can try solid appoarch
for example:
Table S5 Mouse Disease-Phenotype-Gene Sets NIA 10-21-08
//www.grc.nia.nih.gov/branches/rrb/dna/data/table-s5-a.txt
These data and gene sets are for RESEARCH PURPOSES ONLY.
They are speculative and should be used for exploratory research purposes
only.
Please cite this Reference- Zhang, Y et al. Medical Genomics 2010
more
//www.ncbi.nlm.nih.gov/pubmed/20092628
scope |
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z*t
发帖数: 863 | 14 re
提个budget的
wgbs很贵，俺们实验室是一个sample一条lane以达到足够的coverage
errbs可以pool多个sample在一条lane |
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A******u
发帖数: 61 | 16 那肯定是eRRBS了。
其实你们可以自己建库，只给BGI测序就行。
测序结果也可以自己分析的，不难。
with
I
Run
it |
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k*****o
发帖数: 148 | 17 甲基化还是做RRBS或者WGBS吧，甲基化芯片上的探针主要针对promoter，而promoter的
甲基化不是DNA甲基化动态变化最大的区域。 |
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