g*********3
发帖数: 177 | 1 小弟第一次发帖，请教下各位~
如果要进行liver metabolic flux analysis，现在比较通用的技术是？这些技术是否
够sensitive能够反应诸如pyruvate,cAMP,glucose等等这些比较重要metabolites的变
化。
谢谢各位。 |
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b*******e
发帖数: 288 | 2 moonlighting function?
以前读到一篇文章，讲得是pyruvate carboxylase的没有催化功能的一端对酵母的
alcohol oxidase的组装和运输起很大的作用。印象超级深刻啊。
觉得可能很多现在不好解释的现象以后都可以另辟蹊径来解释。 |
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h******y
发帖数: 351 | 3 Actually, tumor cells do not prefer to use oxygen. The so-called Warburg
effect is the observation that most cancer cells predominantly produce
energy by a high rate of glycolysis followed by lactic acid fermentation in
the cytosol, rather than by a comparatively low rate of glycolysis followed
by oxidation of pyruvate in mitochondria like most normal cells.
Unlike what we would think, malignant rapidly-growing tumor cells typically
have glycolytic rates that are up to 200 times higher than thos... 阅读全帖 |
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M******n
发帖数: 92 | 5 Warburg effect 里起作用的Pyruvate dehydrogenase kinase作为cancer的target怎么
样？ |
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l**********1
发帖数: 5204 | 10 Move On LZ
this kind of topic never get NDA or FDA approved
it is just waste time and Fundings.
from that 一个新的单克隆抗体治疗带肿瘤裸鼠的问题
if you did not used multi novel 单/poly 克隆抗体治疗带one type 肿瘤裸鼠
pls refer:
发信人: luminb (Gooder), 信区: Biology
标 题: Re: 大家说说Cancer cell metabolism吧，这个越来越火啊！
发信站: BBS 未名空间站 (Sun Jan 6 22:48:08 2013, 美东)
我已经发现有个规律了。大牛发些东西，打脸的常常是药厂。为啥，大牛做的药厂纷纷
跟风啊，然后一看，NND不是这么回事。很多情况下，大家也就算了，有的就会跳出头
，发个打脸的文章。呵呵。
http://www.mitbbs.com/article_t1/Biology/31748337_0_3.html
or
and
M2 isoform of pyru... 阅读全帖 |
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d*******s
发帖数: 24 | 11 感觉完全取决于细胞是增殖期 还是休眠期
增殖期的细胞会依赖glycolysis 癌细胞属于这一类
休眠期的细胞会依赖catabolic mechanism 比如pyruvate oxidation(TSC) fatty
acids oxidation(LT HSC)
分化期的细胞会依赖OXPHO
所以，细胞的内在需求决定了代谢方式。 这种内在需求又是有微环境保障的。 |
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h*******o
发帖数: 4884 | 12 in vitro or in vivo?
in vitro it is easy
You can use hexokinase inhibitor, such as 2-deoxyglucose,
You can use PDH activator, such as dicholoracetate
You can use Pyruvate transporter inhibitor, Thiazolidinediones
There are so many ways to manipulate cellular bioenergetic flux (glycolysis
or Mitochondrial OXPHOS), some may require digitonin or other similar
detergent to permeablize plasma membrane. |
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w*****s
发帖数: 230 | 13 附件是主治大夫的reasoning(email回复，红字)， 他的水平我们还是很佩服的，因为
他已开始猜了个病就和我们宝宝症状非常一致，可惜后来发现不是；而且他不是代谢病
专家。
问题是坚持宝宝不吃长链脂肪酸的奶粉后，他奇迹般地改善了；完全出乎医生们的预料
。我感觉医生列的是常见的代谢病，而我们感觉宝宝吃长链脂肪酸似乎是慢性中毒，大
概需要一个月左右才能体现出来。因为过程很慢，我们怀疑很多方法查不出来。
我们最大的问题是如果是这种病怎么查？不能确认的话他一辈子吃饭都受影响，基本也
就很难出门时间长一点了。尤其是他的肝已经在要换的边缘。医生们现在改口说他的病
程都是偶然，可我们不能同意。为什么每次都是长链吃一段时间就得病，尤其是这次停
了鸡蛋后马上改善。。
谢谢大家。 （下面是文字，可惜设置不成红色）
Q: Our biggest concern is the reason of his liver damage.
A: The biopsy shows severe fibrotic changes and tells us that this is not a
liver that ... 阅读全帖 |
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t******n
发帖数: 33 | 14 Yes, I used E:T=10:1. NK cells=100,000cells/well, target cells=10,000cells/
well in 96-well round bottom plates.
After overnight culture in RPMI1640+GlutaMax+Sodium Pyruvate+10%FCS+10ng/ml
IL-2 at 1.0x10e6cells/ml, I mixed the NK cells with target cells and co-
cultured for 2 hours. I got ~100% killing of target cells even without any
antibodies.
When I removed IL-2 from the medium and cultured NK cells overnight, I got
the same problem:~100% killing after co-culture with target cells for 2
hour... 阅读全帖 |
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X******n
发帖数: 914 | 15 Lack of Evidence for PKM2 Protein Kinase Activity－Molecular Cell
Highlights
•PEP-dependent phosphorylation was assayed in cell lysates using
radioactive PEP
•Trace adenine nucleotides can confound PEP-dependent phosphorylation
assay results
•PEP-dependent phosphorylation is rare and is not dependent on PKM2
•We are unable to detect PKM2 protein kinase activity in a variety of
contexts
Summary
The role of pyruvate kinase M2 (PKM2) in cell proliferation is controversial
. A... 阅读全帖 |
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j*b
发帖数: 341 | 16 读了一下文章， 有五篇文章做出过蛋白激酶活性（四篇老中一片老韩）
最先说PEP能当ATP用的，就是这篇文章的作者本人。
跟风跟到马蹄上了？？
PKM2 phosphorylates MLC2 and regulates cytokinesis of tumour cells. Nat
Commun. 2014
SAICAR induces protein kinase activity of PKM2 that is necessary for
sustained proliferative signaling of cancer cells.
Mol Cell. 2014
PKM2 regulates chromosome segregation and mitosis progression of tumor cells
. Mol Cell. 2014
PKM2 phosphorylates histone H3 and promotes gene transcription and
tumorigenesis. Cell. 2012
Pyruvate kinase M2 reg... 阅读全帖 |
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s**x
发帖数: 468 | 18 Award List:
aii
alligator
aniee
astro
atu2010
befast
beingfly
bulawann
caesar1
catchafish
cdxt
codycody
crashinfo
elvinGG
erjie
EUV
fangxuanling
feefly
forgetever
GDI
geon
ggdd7909
gnrhrules
Goodgood2010
gracesyy
guaichong
HLLL
hpy2006
hsh
hudie
icycream
icycream
isis1414
jjneimei
joycee
kittymao
lancy2008
lovelycaicai
lucky1986
luxilon
makejian
maodaren
maomao11
mingzhengtan
mmtmmt
monkeylady
msq
nancyhaha
NBAFan007
ocean11
ohmygodish
oriclechao
ourustc
ovoneL
OXGBIX
paotangde
peopleocean
pyruv |
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s**********r
发帖数: 178 | 19 1C Ca2+ activates muscle glycogen phosphorylase
2C pyruvate can not enter the TCA cycle. |
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g*******n
发帖数: 91 | 20 多谢hmis，但是 SHUTTLE说的不是glycolysis产生NADH的ATP吗 如果这样 glycolysis应该在内啊
TCA本身产生24个ATP 固定的 再加pyruvate产生的2NADH,总共30个ATP，无所谓shuttle不shuttle吧 |
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M****a
发帖数: 577 | 21 1Glucose-----2ATP+2NADH (from glycolosis)+2NADH(from pyruvate--acetyl CoA)
+6NADH+2FADH2+2GTP (from TCA cycle)=== 2ATP+2GTP+10NAD+2FADH2
1NADH--2.5ATP, 1FADH2--1.5ATP, malate-aspartate shuttle不消耗ATP，算起来就是
32，Stryer的Biochemistry很早就这么说了。
老版本的生化书 1NADH--3ATP, 1FADH2--2ATP，算出来就是38. |
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s*****1
发帖数: 273 | 22 This is my collection over the last two years. Hope it will be of some help
to other test-takers.
gastrogastrostomy 胃胃吻合术;
genioglossus 颏舌肌;
glyceryl guaiacolate 愈创木酚甘油醚;
glycyl glycine N-甘氨酰甘氨酸
Threonine 苏氨酸［氨基酸类药］
Propionyl-CoA carboxylase 丙酰基-CoA羧化酶;丙酰辅酶A羧化酶
ophthalmoplegia n.眼肌麻痹,眼肌瘫痪;以眼的运动神经麻痹为特征的疾病;此病往往
发生于重症肌无力、肉毒中毒、维生素B1缺乏和颅内神经疾病.
Ketoglutarate 酮戊二酸
Wernicke center 韦尼克中枢(言语中枢〕
gluconeogenesis 糖原异生[作用];糖异生
Pyruvate 丙酮酸盐;丙酮酸
carboxylase 羧化酶
Acetyl 乙酰
avidin 阿维丁;抗生物素蛋白;卵白素;亲和素〔分〕
Alopecia |
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h**********r
发帖数: 10 | 23 I have one question about how many ATPs are produced for one molecule of Glu
to be aerobically metabolized.
In FA, the answer is 32 ( or 30) depending on which shuttle is used to
transfer NADH.
Is this right?
But my calculation is 38 or 36.
glycolysis: should produce 2 NADH+ + 2 ATPs ( substrate level
phosphorylation 4ATP---2 ATP consumed)
From pyruvate to Acetyl CoA: 2 NADH produced
During TCA: 6 NADH+2FAD+2GTP
so the total should be ( malate shuttle): 10 NADH * 3+ 2 FADH* 2+ 2ATP +
2GTP=38
Am |
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l*********0
发帖数: 85 | 24 This is a very old question.
Uses FAD as a prosthetic group (FAD→FADH2 in the first step of the reaction
) in the enzyme,[4]
generates the equivalent of 1.5 ATP
generates NADH (equivalent of 2.5 ATP)
Description Reactants Products
The sum of all reactions in the citric acid cycle is: Acetyl-CoA + 3 NAD+ +
Q + GDP + Pi + 2 H2O → CoA-SH + 3 NADH + 3 H+ + QH2 + GTP + 2 CO2
Combining the reactions occurring during the pyruvate oxidation with those
occurring during the citric acid cycle, the followin |
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l********y
发帖数: 2295 | 25 【 以下文字转载自 Pre_Resident_Club 俱乐部 】
发信人: lovelypony (lovelypony), 信区: Pre_Resident_Club
标 题: steps学习的口诀
发信站: BBS 未名空间站 (Fri Oct 22 21:56:46 2010, 美东)
响应老刀的号召，冒个小泡。有很多难记的东西，编过一些口诀，先贡献一个，回头有
空了再慢慢添。大家有好的口诀也都来分享，这样大家都省时省力。
先贡献一个pony比较经典的口诀，认识pony的都会的，呵呵。
for step1:
trinucleotide repeat expansion diseases:
My Ex is Hungry on Fri.
My (大家都Call Tall Girl, CTG)=Myotonic dystrophy
Ex (比较肉麻的Call Ge Ge, CGG)=fragile X syndrome
Hungry (点了个鸡蛋Call An Egg, CAG)=Huntington's disease
Fri (大家AA制的，Go AA, GAA)=Fried... 阅读全帖 |
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l********y
发帖数: 2295 | 26 做题中场，贡献一下小宝的故事。这个貌似答应了kaye斑竹的。pony压箱底的，呵呵。
小宝的7个LP：
大LP（VitB1,thiamine）
二LP(VitB2,riboflavin)
三LP（Vit B3, niacin）
四不吉利，没有四老婆。
五LP（Vit B5, pantothenate）
六LP（Vit B6, pyridoxine）
七LP（Vit B7, biotin）
小LP（Vit B12, cobalamin）
大LP（VitB1,thiamine）:派（Pyruvate dehydrogenase）阿铜（alpha-ketoglutarate
dehydrogenase）从侧门（branched-chain AA dehydrogenase）搬运铜（
transketolase）换私房钱。好像大LP都喜欢攒私房钱，嘻嘻。
二LP擅长flirt（联想到riboflavin,中间有个fla，还联想到FAD/FMN）。flirt太多了唇干裂（Cheilosis），媚眼抛多了角膜都血管化了（Corneal vascularization）。
三LP是lesbian，p... 阅读全帖 |
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c****i
发帖数: 40 | 27 不忍心看着你焦急的心情，其实这里很多人都愿意帮忙，但是由于种种原因，无法给你
回复。
就以一般性的回复几句，首先你给的非常有限的病史体检化验结果等等，所以基本上没
人能在这里给你一个诊断。我search了一下，下面是链接以及原文，希望对你能有帮助
。另外，也不要小看国内的医生，有很多是不错的。
点击链接可以发现更多的内容。
http://emedicine.medscape.com/article/958739-overview
History
Despite the extensive differential diagnoses of splenomegaly, careful
history taking and physical examination, along with a CBC count and liver
function tests, often help in narrowing the list of possible causes. The
history should include attention to the following importan... 阅读全帖 |
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i**********d
发帖数: 853 | 28 首先声明：我的分数很普通，所以这个帖子也没有任何炫耀的意思，只是我在考前
，每天都来看看有没有考经，也经常拿自己的模考成绩和考经中的对照一下，看看自己
复习的效果，从前辈的考经中学到很多。所以，本贴得唯一目的是：希望能给还在准备
step1的朋友们一点提示，吸取我的教训，都考出一个好成绩。
正文：
我是8/06/2012考的Step1，昨天（8/29/2012）早上9:19am拿到成绩---242/85，还
好，和自己估计的成绩240 +/- 5基本一致。万分感谢我的家人的理解和支持，十分感
谢曾经一起学习的战友们和校友们的鼓励和帮助，感谢麦地版的前辈们的考经和经验介
绍。曾经许诺一旦成绩上了230，就写一篇考经，下面就把我的备考经历和一点心得体
会向麦地版的战友们作一汇报，也算是对自己这段人生的一个总结：
一、题库、模考及考试成绩+时间：
UW qBank: 正确率最早25%，考前80-90%
Kaplan qBank: 只做了解剖、遗传，没有统计正确率
Dec 18, 2011, Form3 --- 350
Feb 25, 2012， F... 阅读全帖 |
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i**********d
发帖数: 853 | 29 Jeff的75条口诀：
1).
Chloramphenicol inhibits 50S peptidyltransferase.
喷着氯气的ram(羊)拦住(抑制) 50岁的亚裔太太搬家(转移)(50S亚基肽键转移酶)
Clindamycin & chloramphenicol block peptide bond formation.
克林顿用氯气 吓唬老婆 --> 防止太太贱(肽键)形成
Aminoglycosides bind 30S and inhibit formation of the initiation complex and
cause misreading of mRNA.
Amigo绑架了30S亚裔,抑制了她婚姻的起始,造成她男友的误读
Macrolides block translocation.
带着大沿帽子的人（城管？）挡道--> 不让别人换地方translocation
2).
下肢淋巴回流：
小隐隐于城外蝈蝈窝棚，大隐隐于城内富人水沟
（小隐收集小腿外侧淋巴液，注入腘窝；
大隐收集下肢内侧淋巴液，注入腹股沟；）
3)
imprinting diseas... 阅读全帖 |
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i**********d
发帖数: 853 | 30 首先声明：我的分数很普通，所以这个帖子也没有任何炫耀的意思，只是我在考前
，每天都来看看有没有考经，也经常拿自己的模考成绩和考经中的对照一下，看看自己
复习的效果，从前辈的考经中学到很多。所以，本贴得唯一目的是：希望能给还在准备
step1的朋友们一点提示，吸取我的教训，都考出一个好成绩。
正文：
我是8/06/2012考的Step1，昨天（8/29/2012）早上9:19am拿到成绩---242/85，还
好，和自己估计的成绩240 +/- 5基本一致。万分感谢我的家人的理解和支持，十分感
谢曾经一起学习的战友们和校友们的鼓励和帮助，感谢麦地版的前辈们的考经和经验介
绍。曾经许诺一旦成绩上了230，就写一篇考经，下面就把我的备考经历和一点心得体
会向麦地版的战友们作一汇报，也算是对自己这段人生的一个总结：
一、题库、模考及考试成绩+时间：
UW qBank: 正确率最早25%，考前80-90%
Kaplan qBank: 只做了解剖、遗传，没有统计正确率
Dec 18, 2011, Form3 --- 350
Feb 25, 2012， F... 阅读全帖 |
|
i**********d
发帖数: 853 | 31 Jeff的75条口诀：
1).
Chloramphenicol inhibits 50S peptidyltransferase.
喷着氯气的ram(羊)拦住(抑制) 50岁的亚裔太太搬家(转移)(50S亚基肽键转移酶)
Clindamycin & chloramphenicol block peptide bond formation.
克林顿用氯气 吓唬老婆 --> 防止太太贱(肽键)形成
Aminoglycosides bind 30S and inhibit formation of the initiation complex and
cause misreading of mRNA.
Amigo绑架了30S亚裔,抑制了她婚姻的起始,造成她男友的误读
Macrolides block translocation.
带着大沿帽子的人（城管？）挡道--> 不让别人换地方translocation
2).
下肢淋巴回流：
小隐隐于城外蝈蝈窝棚，大隐隐于城内富人水沟
（小隐收集小腿外侧淋巴液，注入腘窝；
大隐收集下肢内侧淋巴液，注入腹股沟；）
3)
imprinting diseas... 阅读全帖 |
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d***i
发帖数: 93 | 32 Q:a 25y/o man is lost in the desert for 1 week with an ample supply of water
but no food. Which of the following changes in enzyme activities and
regulatory molecule concentrations in liver is most likely in this pt?
（1）Fructose 2,6-bisphosphate：increase or decrease
（2）Glucose 6-phosphatase： inc or dec
（3）Phosphoenolpyruvate Carboxykinase： inc or dec
（4）Pyruvate Kinase： inc or dec
我选了全降低，错了！查了网上解释，大多数说（1）（4）因为参与glycolysis，所以
降低，（2）（3）因为参与gluconeogenesis所以升高。
但有一个解释说应该只有（2）升高。因为starvation >3days，... 阅读全帖 |
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i**********d
发帖数: 853 | 33 75条口诀：
1).
Chloramphenicol inhibits 50S peptidyltransferase.
喷着氯气的ram(羊)拦住(抑制) 50岁的亚裔太太搬家(转移)(50S亚基肽键转移酶)
Clindamycin & chloramphenicol block peptide bond formation.
克林顿用氯气 吓唬老婆 --> 防止太太贱(肽键)形成
Aminoglycosides bind 30S and inhibit formation of the initiation complex and
cause misreading of mRNA.
Amigo绑架了30S亚裔,抑制了她婚姻的起始,造成她男友的误读
Macrolides block translocation.
带着大沿帽子的人（城管？）挡道--> 不让别人换地方translocation
2).
下肢淋巴回流：
小隐隐于城外蝈蝈窝棚，大隐隐于城内富人水沟
（小隐收集小腿外侧淋巴液，注入腘窝；
大隐收集下肢内侧淋巴液，注入腹股沟；）
3)
imprinting disease: ch... 阅读全帖 |
|
i**********d
发帖数: 853 | 34 75条口诀：
1).
Chloramphenicol inhibits 50S peptidyltransferase.
喷着氯气的ram(羊)拦住(抑制) 50岁的亚裔太太搬家(转移)(50S亚基肽键转移酶)
Clindamycin & chloramphenicol block peptide bond formation.
克林顿用氯气 吓唬老婆 --> 防止太太贱(肽键)形成
Aminoglycosides bind 30S and inhibit formation of the initiation complex and
cause misreading of mRNA.
Amigo绑架了30S亚裔,抑制了她婚姻的起始,造成她男友的误读
Macrolides block translocation.
带着大沿帽子的人（城管？）挡道--> 不让别人换地方translocation
2).
下肢淋巴回流：
小隐隐于城外蝈蝈窝棚，大隐隐于城内富人水沟
（小隐收集小腿外侧淋巴液，注入腘窝；
大隐收集下肢内侧淋巴液，注入腹股沟；）
3)
imprinting disease: ch... 阅读全帖 |
|
S**********8
发帖数: 30 | 35 Thanks a lot!
Happy New Year~
, centaur, clavichord, coolhunter, DabaoSODBoy, dcw, Discovery, dongchengqu,
Drake, drew, enantiomers, FRJJ2009, geon, guaiguaicong, haiduc, happydoudou
, icbchsbc, isisl414, jjneimei, Kakaa, kicolly, largewolf, LingLingCool,
lingyunxie, Logan601, LuckygirlIam, makejian, ManCity, maoyi, Maxstudio,
Mercedes, meso, Mobot, mochamocha, nash1031, normanwu, oriclechao, paotangde
, pyruvate, Reduce, reshaping, scoreboard, shopper2: , steve, superbao,
tianyumi, Tody, Tomos |
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m*********k
发帖数: 10521 | 36 [Wisdom] sheepchicken Dec 24 ● merry christmas
66*59=3894
成功奖励 66 伪币的用户: March99, aii, akju, aniee, anniee, aOzu, arthury, bactive79, banda, baozi09, bdbd, codycody, dawangtoo, dcw, futurehw, guaichong, guaiguaicong, guanjiu, haiduc, HLLL, Hotfly, isis1414, jaff1973, jjmeimei, jym2307, kicolly, lanzhu, largewolf, leonana, linglingQ, lingsu, litocoffee, loneness, makejian, maodaren, maomaopaopao, nosema, oriclechao, paotangde, peopleocean, Physmolecule, puppeteer, purity, pyruvate, questi |
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m*********k
发帖数: 10521 | 37 [Detective] mind Apr 28 ● 啊，第一次从政了
10*62=620
成功奖励 10 伪币的用户: Ametc9, aniee, anniee, anywhere, atu2010, bactive79, bell007, bond7, churchance, coolhunter, crashinfo, daffodilly, dawangtoo, daydream000, ddd0069, doomed, erjie, Fivemao, flyinhill, Goodgood2010, guaichong, hinterlandy, honde, internania, isis1414, jjmeimei, jjneimei, jokeyzhu, joycee, liangzhi, lxinzheng, lyufeng, marlie, metalfan, mind, nosema, ocean18, oloolo, oriclechao, paotangde, parfume, pukapuka, pyruvate, |
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m*********k
发帖数: 10521 | 38 [Family] T76879297 Jun 16 ● 端午节粽子
10*56=560
成功奖励 10 伪币的用户: T76879297, alvdena, aniee, atu2010, bactive79, baozi09, churchance, didiwo, ellenecho, erjie, footbook, geon, Goodgood2010, greenmom, guaichong, hhy9602, HLLL, hwlv, isis1414, jjmeimei, jjneimei, kat80, kicolly, lamprey, lilycola, liuzhu, Lotusfly, lyufeng, niuniu522, nosema, oriolechao, OXGBIX, paotangde, patrickcy, providential, psychowolf, pyruvate, realstar, ruoli, salut, scoreboard, shaya, SmileLilli, soyjoy, summer0 |
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m*********k
发帖数: 10521 | 39 [JobHunting] mandman Aug 30 ● 新版务done上任 五十个包子
10*50=500
成功奖励 10 伪币的用户: aniee, anniee, baozi09, Beijing, Belichick, bigbird11, caopp, chaiy, dizhu2, done, e13369, emstat, futurehw, GetThere, goodjoboffer, happyyz, hawkking01, hunter33, isis1414, Kakaa, karlcui1, koalakoala, leadensky, litchiwl, lostheart, LuckygirlIam, luhonghao, ManshengChen, Mosis, nosema, oldfish, Oly, PetTurtle, PlanetLab, Pontiff, pyruvate, qianmen, relaxrelax, sheepsheep, Statwest, svncheckout, swh, Tody, wal |
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m*********k
发帖数: 10521 | 40 [Seattle] belle Sep 22. ● 发100个中秋包子~祝大家节日快乐
10*100=1000
成功奖励 10 伪币的用户: Notsaw, simon1000, xjtuboy, zhaor, stripper, bemoonlight, MindCruncher, gochiefs, kunkun, graceful, aou, lancy1982, Wensi, pinkgaga, Muro555, lolhaha, nosema, amy2009, likefishing, calla1118, yumeiren918, yoyo200261, pyruvate, VichyBoy, oriolechao, colico, doudouk, happykitty, guaichong, windcloud, yccyccy, qiyueyue, lxinzheng, saintknight, chu, baozi09, santorini, chibaozi78, bactive79, lantian2010, sanjie, dafe |
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m*********k
发帖数: 10521 | 41 [Seattle] belle Oct 1 ● 61个包子～庆国庆～还是周末～天
10*61=610
成功奖励 10 伪币的用户: stripper, getty, yumeiren918, kenny008, xiwest, Great, ohThree, colico, PInce, Rayshir, Notsaw, happykitty, pyruvate, tocheer4, juliayin, santorini, chibaozi78, bluebusi1, dameizi, forgetthem, lancy1982, FERN2007, iceburg, aniee, simon1000, erjie, guaichong, times, daiyatou, dmqpw, EmeraldFish, jaycheng, lumpkin, GillB, lyufeng, sky, ffdt, shining, scoreboard, graceful, KennyD, paladinalpha, Ilovebluesky, flyingToma... 阅读全帖 |
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m*********k
发帖数: 10521 | 42 [USANews] kicolly Nov 3 ● 难得大家都在
11*100=1100
成功奖励 11 伪币的用户: cougarjx, mathematica, mplus, macrowave, littlecookie, gev, makejian, meilefu, winning2008, Spurs, baozi09, smts, Raistlin, chenshan2010, lxinzheng, apex2009, oriolechao, cairn, windcloud, singularity, ada0001, goforward, Augustine, dafende, funnymall, royalex2009, drew, Rickgocolts, Goodgood2010, alderson, LiWan, summitzf, dingqing1981, AUV, Tody, hopeusw, dadonggua, baleC, feebe, fanzhuce, pathdream, summer05, Raikkonen, ... 阅读全帖 |
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h*h
发帖数: 27852 | 43 注：1）版面活动：手续费无；2）代发包子：手续费10%:
1）版面活动：手续费无
代发版面/ID:
Salon
代发事由(主题标题或链接):
诺贝尔和平奖
伪币金额:
574ID， 10伪币/ID
奖励/代发包子数量:
10*574=5740
直接从版面扣除，谢谢~
名单：
a00
aafa
abcxyz2046
ada0001
affymm
agabear
aisinfox
aladdin
albball
albundy08
allence
Alton
alvincy79
AmberMM
analogg
ananan
andy123
angelina2009
angelwinter
aniee
annie
AnnieAnita
antholy
antony
apex2009
apple1105
Areyousure
armilla
ARSU
art
asj9628
Atascadero
atu2010
babybaby2010
baoyuhong
baozi09
bbbear
Beida101
beiye
BeLami
bigmountain
Biotrapped
bison... 阅读全帖 |
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m*********k
发帖数: 10521 | 44 [WBCenter] hsh Nov 4. ● 民主沙龙（Salon）版申请代发包子
10*574=5740
成功奖励 10 伪币的用户: a00, aafa, abcxyz2046, ada0001, affymm, agabear, aisinfox, aladdin, albball, albundy08, allence, Alton, alvincy79, AmberMM, analogg, ananan, andy123, angelina2009, angelwinter, aniee, annie, AnnieAnita, antholy, antony, apex2009, apple1105, Areyousure, armilla, ARSU, art, asj9628, Atascadero, atu2010, babybaby2010, baoyuhong, baozi09, bbbear, Beida101, beiye, BeLami, bigmountain, Biotrapped, bison, bitmap, blot,... 阅读全帖 |
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m*********k
发帖数: 10521 | 45 [ebiz] kelly9988 Dec 13 ● 明天又需要大家祝福 这里发包子很灵验 再发66个
10*66=660
成功奖励 10 伪币的用户: N007, coolyun, soimbox, mm63, zhanglong0, lovemocca, SinoKnight, billred, noodles, adots, ilovecat, FakeMask, kap, zy0100, huliweiba, smart1118, mengguo, hwsim, kinson, shiwit, taotao701, folk, papabear, ryana, flemns, EZbiz, crossya, andydan, pl6666, Pizzz, o2, mcheng82, feifei126, newmanalways, Sickdeal, jajajkjk, Winglet, loneness, pixie, CODEN, nancylem, dali095, iouxx, zkjeffrey, ahaha, ming2008, nosema, smallc... 阅读全帖 |
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m*********k
发帖数: 10521 | 46 [Family] T76879297 Dec 27 ● 2011新年包子
10*399=3990
成功奖励 10 伪币的用户: boysa, victoh, Zzl, snowxy, LiWan, h60375, ladyred, upsidedang, itiger, GEENU, riverabc, everafter, emilymis, siebens, shinai, zoja, foxcry, didiwo, cappucino, fionana, coalpilerd, Haowan888, axyxy, Babel, Lotusfly, donthurtme, lovebaby2009, bigbigapple, happybaby111, happy6666, koox, YDXH, lifesmile, jwmm, lllyyyt, variance, ysc123456, vonda, daxue, linklisa, viviwu, shark, sillybird, cyw, changling, feelfree, daxu06, dr... 阅读全帖 |
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m*********k
发帖数: 10521 | 47 [Basketball] pandamalone Jan 1 ● 新年快乐!
20*200=4000
成功奖励 20伪币的用户: aaayyy, Drake, CTU, K24, dreamsun, jewwel, chahu, Catskill, goubeibei, Portugal, sioc, TheAnswer, andysun, vvinter, xiaoxixiao, s3, esperlada, nimble, starfallymh, chilema, nocworker, haleluyak, grapeshot, hh, mitshui, LiWan, EOC, happyfather, zirconia, phenixsun, cnbuff410, Yolanda11, yueke, fatbeaver, arity, roodmurning, ldxk, nne3016, Spain, shuihaizi, Manuel, bmwcar, usfish, littlefool11, playonpaper, smokefly, WindyHan, ... 阅读全帖 |
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m*********k
发帖数: 10521 | 48 [Stock] kingcrown Dec 31 ● 发888个包子，祝同志们朋友们在新的一年里发发发！
10*728=7280
成功奖励 10 伪币的用户: rafa823069, carlx, veryqq, joa, xfengyu, water77, sc527, iCure, sinsin, iGOOGLE, hardtime123, myhoo, muse55, yingwu, nicedayhere, HiQ, leehp327, swh, kumingren, miniid, nene415, imgunter, ChenHil, Gocamry, laocat, hlwn, zhchong, Gianna, shyx, MarchRabbit, ipeb, springtree, ijoy, ryanwang, focus8, runnnn, GooPe, JSIS, Ranma98, regsoft, Riven, retroplan, AE, allence, septin, istone, adventure, GoRockets, upthecloud... 阅读全帖 |
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m*********k
发帖数: 10521 | 49 [Nextgeneration] mitbbx Dec 31. ● ★宝宝版祝大家幸福嗨皮2011★
372*20=7440
成功奖励 20 伪币的用户: TXSunshine, leaflee, LePetitPpn, Catback, geon, mitbbx, didiwo, crysknife, winning2008, Sigma, Muro555, zaq123321, wangm019, lyve1, happybaby111, bonymb, feitianren, moonflower7, lelala, Lotusfly, passwd, qingq, lovebaby2009, Iwanttosayhi, knli79, mmitbbs, Babel, Chapterxi, ttgl, Hemerocallis, lakefrog, Viaaa, Yolanda11, APRIL83412, candy1980, yizhitumao, nosema, BooBair, ynred, armilla, happy6666, lawseeker... 阅读全帖 |
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m*********k
发帖数: 10521 | 50 [Basketball] pandamalone Jan 11 ● s散尽家财发不限量包子祝贺我国四代隐身重型战
10*400*1.1=4400
成功奖励 10 伪币的用户: loveclausius, dannyfulgent, Zeeeero, huamei91106, lovelaker, sckyo, Belichick, wallwallwall, YaoReturn, lg, ManU, iversonkxmd, abrahamfeng, joshuazzx, leftHand, usfish, lxinzheng, paladin, huangshiren, bruclee, luxilon, Drake, TINS, longxi, ZhenZhi, lele1986, bearfire, woshixiao, dreamdate, laomaomao, trigold, TABASCO, larrabee, ldxk, Gg0701, zhenhuay, qidamm, iso35, angelsun, bear, s3, woailanqiu, tan09... 阅读全帖 |
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