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全部话题 - 话题: purifed
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m***c
发帖数: 637
1
招聘:世界500强上海研发中心诚聘以下物理化学生物医药海外留学人士。
If you are interested, please email your CV to d****[email protected] with the
position that you are applying as the title.
Assistant Research Scientist-Food Science
Assistant Research Scientist-Polymer
Assistant Research Scientist-Rheology in Deposition
Assistant Research Scientist-Water Purification
Assistant Research Scientist –Deposition
Product Development Technologist-Skin GDC护肤类产品开发研究(For Skin
Category)
Research Associate (Medical & clinical trial management)
As... 阅读全帖
l****o
发帖数: 32
2
来自主题: JobHunting版 - Amgen internship opportunity
Please apply for it online ASAP if you are interested in this opportunity
and I will recieve your resume right after that. Thanks.
Auto req 30057BR
Job Posting Title Grad Intern - Operations
Career Category College Job
Employee Subgroup Seasonal FT
Country (State/Region) United States - California
Location (City) US - CA - Thousand Oaks
Amgen Job Description Amgen’s 10 – 12 week internship program offers
meaningful project experiences that impact patient’s lives. This... 阅读全帖
r********r
发帖数: 20
3
Positions (2) are available immediately for chemists with MS & Ph.D.
majoring in organic chemistry.
The ideal candidate must have 2-5 years experience in organic synthesis,
familiar with purification and structure determination.
Job responsibilities: design/optimize synthetic routes,, scale up
gram to kilogram. Expert on characterization (NMR, LCMS, GC, HPLC) and
purification are also required.
Good communication (both written and verbal),and team oriented spirit.
Comprehensive compensation and
c*******s
发帖数: 17
4
Please send your resume to [email protected], if interested.
Thanks!
Senior scientist in Protein Department
Successful applicant of this position will join in our protein department to
develop and understand protein expression and purification. The scientist
will be in charge of developing new technologies or platforms in the field
of protein expression and purification. In addition to technology
development, other duties may include cell culture, method development, data
analysis and presenta
m***c
发帖数: 637
5
招聘:世界500强上海研发中心诚聘以下物理化学生物医药海外留学人士。
If you are interested, please email your CV to d****[email protected] with the
position that you are applying as the title.
Assistant Research Scientist-Food Science
Assistant Research Scientist-Polymer
Assistant Research Scientist-Polymer
Assistant Research Scientist-Rheology in Deposition
Assistant Research Scientist-Water Purification
Assistant Research Scientist –Deposition
Product Development Technologist-Skin GDC护肤类产品开发研究(For Skin
Category)
Research Associate (Me... 阅读全帖
m****s
发帖数: 18160
6
【 以下文字转载自 Postdoc 讨论区 】
发信人: mrmar (mrmar), 信区: Postdoc
标 题: One tenure-track assistant professor (co-PI) position (Gang Cai at USTC)
发信站: BBS 未名空间站 (Sun Feb 24 20:49:52 2013, 美东)
帮朋友转发,请直接联系文中的E-mail
One tenure-track assistant professor position is immediately available for
three years in the group of Dr. Gang Cai (University of Science and
Technology of China, Hefei National Laboratory for Physical Sciences at the
Microscale). This is a co-PI position with an initial contract up to three
year... 阅读全帖
m***r
发帖数: 49
7
帮朋友转发,请直接联系文中的E-mail
One tenure-track assistant professor position is immediately available for
three years in the group of Dr. Gang Cai (University of Science and
Technology of China, Hefei National Laboratory for Physical Sciences at the
Microscale). This is a co-PI position with an initial contract up to three
years and could be extended for a longer time. Successful candidates will
receive a startup package and are expected to perform independent research
in the Cai lab by supervising gradua... 阅读全帖
c*****n
发帖数: 195
8
Position Summary
Work at the Bio5 Oro Valley, AZ. Experience with electrophysiological assays
is required. Priority will be given to candidates with extensive experience
in small molecule drug screening using patch clamp and voltage clamp assays.
Duties and Responsibilities
*Perform ion channel recording.
*Perform drug screening using two-electrode voltage clamp assays.
*Develop antiviral assays.
*Large scale protein expression, purification, and characterizations.
*Collaborate in the in... 阅读全帖
e******6
发帖数: 6
9
Job Description
Cooperation Introduction
GenScript is a contract research organization (CRO) specialized in
biological research and drug discovery services. Ever since its inception in
2002, GenScript has experienced rapid, constant, and organic growth. Now
GenScript has become a leading biology contract research organization (CRO)
in the world, with a global operating team of over 900 dedicated scientists,
staffs.
Headquartered in Piscataway, New Jersey, GenScript has become a leading
Biology C... 阅读全帖
d*****s
发帖数: 5610
10
这个肯定是室内空气质量不好,有Formaldehyde,其实所有房子室内空气质量都比室外要差,可以买一个IQair的air purifer就可以了,$800,这个是最好的purifer,如果觉得贵,可以买Austin Air, Rabbit air, Sharp或者winix的,Rabbit air有可以filter out chemical, VOC的filter,Winix很便宜,但是不一定filter效果好,不过对于Formaldehyde来说,恐怕IQair效果最好。
昏昏沉沉是典型的空气质量不好的症状,反正肯定要买air purifier,检测有什么用?
http://www.air-purifier-power.com/formaldehydedangers.html
m*f
发帖数: 8162
11
是不是该留下的判断依据是这个东西是属于房子的fixture,还是属于seller的
personal property。所有的fixture默认都应该留下除非特别说明。water softener和
purification systems (比如你的RO),绝大多数头脑正常的人都认为water softener
和purification system属于fixture,除非额外说明。比如这个网站上:
http://www.thehillgroupaz.com/2012/home-selling-tips-which-fixt
你要么google一下:is a water softener a fixture
你的realtor真烂啊,什么都不懂。你得和她的老板,也就是她的broker写信。她的
broker有责任出面解决这个纠纷
j*x
发帖数: 425
12
来自主题: NextGeneration版 - moving sale,有买有送,大家快来瞧瞧
准备搬家了,有些东西可能暂时用不到了,希望有需要的朋友来看看,我的zip是11235
有兴趣请站内联系,或发短信打电话至7165258005
1, holmes加湿器 $8
2,honeywell加湿器 $10
http://www.amazon.com/Honeywell-Germ-Free-Humidifier-HCM-350/dp
3,宜家玻璃小桌板,可以放床上搁笔记本电脑,也可以放在地上当茶几 2$*2个
4,fisher price swing 宝宝小时候很喜欢坐,现在大了,不能坐了
http://www.amazon.com/Fisher-Price-Brentwood-Collection-Swing/d 原价$129.99+tax 卖20
剩下的就不拍照片了,直接上链接了
5,walmart买的这个转角玻璃电脑桌 http://www.walmart.com/ip/Black-Glass-Computer-Desk/9193530 online价格$99+tax 卖$20
6,amazon买的仰卧起坐器械 http://www.amazon.com/gp/pr... 阅读全帖
j*x
发帖数: 425
13
来自主题: PennySaver版 - moving sale zip 11235
准备搬家了,有些东西可能暂时用不到了,希望有需要的朋友来看看,我的zip是11235
如有兴趣请站内联系,或发短信打电话至7165258005
1, holmes加湿器
2,honeywell加湿器 $10
3,宜家玻璃小桌板,可以放床上搁笔记本电脑,也可以放在地上当茶几 2$*2个
4,fisher price swing 宝宝小时候很喜欢坐,现在大了,不能坐了
http://www.amazon.com/Fisher-Price-Brentwood-Collection-Swing/d 原价$129.99+tax 卖20
剩下的就不拍照片了,直接上链接了
5,walmart买的这个转角玻璃电脑桌 http://www.walmart.com/ip/Black-Glass-Computer-Desk/9193530 online价格$99+tax 卖$20
6,amazon买的仰卧起坐器械 http://www.amazon.com/gp/product/B001IKHUNQ/ref=oh_details_o00_s00_i00?ie=UTF8&psc=1 原价$65+tax... 阅读全帖
r********h
发帖数: 5638
14
来自主题: PennySaver版 - 闹心的净水器
Reverse osmosis (RO) is a water purification technology that uses a
semipermeable membrane. This membrane technology is not properly a
filtration method. In reverse osmosis, an applied pressure is used to
overcome osmotic pressure, a colligative property, that is driven by
chemical potential, a thermodynamic parameter. Reverse osmosis can remove
many types of molecules and ions from solutions, and is used in both
industrial processes and producing potable water. The result is the solute
is retai... 阅读全帖
m***c
发帖数: 637
15
招聘:世界500强上海研发中心诚聘以下物理化学生物医药海外留学人士。
If you are interested, please email your CV to d****[email protected] with the
position that you are applying as the title.
Assistant Research Scientist-Food Science
Assistant Research Scientist-Polymer
Assistant Research Scientist-Polymer
Assistant Research Scientist-Rheology in Deposition
Assistant Research Scientist-Water Purification
Assistant Research Scientist –Deposition
Product Development Technologist-Skin GDC护肤类产品开发研究(For Skin
Category)
Research Associate (Me... 阅读全帖
b********n
发帖数: 22
16
来自主题: Postdoc版 - New England Biolabs Postdoc Fellowship
Postdoctoral Fellowship – Restriction Enzyme Division – Job Code 1042HZ
The Restriction Enzyme Division invites applications for the position of
postdoctoral fellow. Projects will be part of a basic research program
focusing on restriction enzymes specific to 5-hydroxymethylcytosine. The
project will involve cloning, overexpression, purification, assay-
development, and engineering of such enzymes, and their application to
questions of epigenetics. Research will be undertaken in a highly
coll... 阅读全帖
f*****l
发帖数: 3
17
http://pnnl.jobs/richland-wa/post-doctorate-ra-peptoid-synthesi
Job ID: 304908
Directorate: Physical & Computational Sciences
Division: Physical Sciences
Group: Materials Sciences
.
Job Description
The successful candidate will be a synthetic chemist or materials scientist
responsible for peptoid synthesis, purification and assembly. This
researcher will also be involved in exploiting peptoid assemblies for
applications. Instruments that will be used for peptoid synthesis and
purification includ... 阅读全帖
f******e
发帖数: 541
18
来自主题: Immigration版 - 审稿机会:Journal of Supercritcal Fluids
I am a molecular biochemist with rich research experience in macromolecular
purification and biological function screening, such as polysaccharides, DNA
, RNA and proteins extraction and purification by FPLC,gel filtration,
chromatography, TLC etc.My multidisciplinary expertise can be demonstrated
by more than 10 peer-reviewed papers that were published in leading
biochemistry journals, such as J Biol Chem., Biochem J. , Mol Cell Biol.,
DNA Repair (Amst). , PLoS One. etc.
PS:
M.S., Ph.D., Curren... 阅读全帖
W*******n
发帖数: 4140
19
来自主题: Chinese版 - 我的2011年版找工简历
我的2011年版找工简历
王利民
2014年8月16日
(现注:当时,未列出2010年在Fox Chase Cancer Center的经历;没列出2005年在
Memorial Sloan-Kettering Cancer Center的经历;没列出2006年在陕西师范大学、
2009年在江南大学任教的经历。)
Career Summary:
PhD degree on Chemistry and post-doctoral research on protein
structure in the USA; Teaching (assistance) experience in the USA and
China; Customer service experience in the USA.
Work/ Research:
Jan 2006 - : various work in the USA, China, and home.
Sept. 2003- June 2005: New York State Department of Health
Post-doctorate
... 阅读全帖
j**********2
发帖数: 16
20
来自主题: Boston版 - Positions open in a major pharma
The Biologics division of a major pharmaceutical company is looking for 3
people for the following positions:
Protein purification(1)(Ph.D.): Ph.D in protein chemistry or related field,
5 years experience (industry prefered), specializing in antibody
characterization and purification, ACTA system, HPLC.
Molecular biologists(2)(BS/MS): A minimum of 2 years work experience, PCR,
cloning, Western blot, mammalian cell culture, antibody experience would be
a plus.
If interested, email your CV to: jac
S*****a
发帖数: 63
21
【 以下文字转载自 Pharmaceutical 讨论区 】
发信人: Somalia (Anti-Pirates), 信区: Pharmaceutical
标 题: Protein Chemistry Group Leader position available in Woburn MA
关键字: protein chemistry,antibody,biologics
发信站: BBS 未名空间站 (Fri Jul 7 09:36:00 2017, 美东)
We are looking for an energetic and dynamic hands-on leader with initiative
and a
collaborative spirit to serve as a Group Leader in our protein chemistry lab
. This is a fantastic
opportunity to drive antigen/antibody expression, purification, and QA to
support
v... 阅读全帖
s*******e
发帖数: 740
22
来自主题: PHILADELPHIA版 - 费城startup工作机会 (转载)
【 以下文字转载自 Biology 讨论区 】
发信人: smallbite (一小口), 信区: Biology
标 题: 费城startup工作机会
发信站: BBS 未名空间站 (Sat Feb 3 16:45:54 2018, 美东)
Scientist/Associate Scientist of immunology
Responsibilities:
Primary immune cell isolation and culture
Antibody purification and characterization
DNA vector construction and cell transfection
Retrovirus production and cell transducing
Skills/Requirements
Experience in primary immune cell culture, immune cell-based assays, flow
cytometry, and cell sorting
Experience in retr... 阅读全帖
v***y
发帖数: 7583
23
来自主题: SanDiego版 - [代发]招生物海归一名
公司总部在东岸,在南京附近设了工厂,最近想招一名phd或者post-doc回去,是完全的
海归,并不是同时在美国和国内工作。
工资方面按人民币给,据说会比local水平好一些,但是不能期望按美国工资付
具体职位介绍如下,有意者请站内信联系
Position Senior Scientist in Protein Science
Opening 1
Description Successful applicant of this position will
join in our protein department to develop and understand protein expression
and purification. The scientist will be in charge of developing new technolo
gies or platforms in the field of protein expression and purification. In ad
dition to technology
e*********u
发帖数: 3591
24
来自主题: SanDiego版 - 以后SD 1/3的家庭要喝循环利用水
MAYOR KEVIN L. FAULCONER
CITY OF SAN DIEGO
FOR IMMEDIATE RELEASE
Tuesday, Nov. 18, 2014
CONTACT:
Craig Gustafson at (619) 453-9880 or [email protected]
(function(){try{var s,a,i,j,r,c,l,b=document.getElementsByTagName("script");l=b[b.length-1].previousSibling;a=l.getAttribute('data-cfemail');if(a){s='';r=parseInt(a.substr(0,2),16);for(j=2;a.length-j;j+=2){c=parseInt(a.substr(j,2),16)^r;s+=String.fromCharCode(c);}s=document.createTextNode(s);l.parentNode.replaceChild(s,l);}}catch(e){}})();
/* ]]>... 阅读全帖
x**k
发帖数: 358
25
来自主题: SanFrancisco版 - 一个蛋白纯化的工作的opening
有兴趣的同学站内信箱联系,或把简历直接发到:h r @ s t e m r d . c o m (电邮
地址无空格)
StemRD Inc. has a temporary Research Associate or Scientist position
available immediately
on protein purification projects. Preferred experience includes protein
expression using bacteria or mammalian cells, and protein purification using
various chromatography. Cell culture experience a plus but not necessary.
StemRD is located at Burlingame and produces cutting edge products for stem
cell research, including recombinant human iPS pr
j***a
发帖数: 10844
26
来自主题: WashingtonDC版 - DC Hiking Trails
Hiking and Backpacking Gear and Tips
I've gotten several e-mails about what gear is necessary for hiking and
backpacking. There are 100s of vendors out there selling different qualities
of gear at different prices. To try to evaluate them all here is impossible
and beyond the scope of this website. Instead I've chosen to list the most
important items you'll need and what features to look for and avoid. I
strongly recommend visiting one of the many on-line outfitters and doing
some comparison sho... 阅读全帖
a********r
发帖数: 538
27
来自主题: Travel版 - 优胜美地大战馒头山
water purification pills
water purification filter
Iodine tablets等等
哎,我也不想买劣质阿。运气不好。或者说有可能天太热,变质了。
后来我们4个都肚子痛,我想不会4根都同时是坏的吧。否则就运气太差了。更有可能变
质了。
我们是除了加得乐吃点糖以外什么都没吃! 就是在吃了那个bar后不久,4个人都有反应,
3个实在熬不住拉肚子去了,唯一的能量也没了...
平时在学校超市买的snack bar, power bar, 去gym运动前吃一根,从来没出过问题。
所以不用太担心呵呵。食物都放cooler里如果有条件。我们以为power bar是干的零食
,没当回事,没放cooler.
f****p
发帖数: 18483
28
来自主题: WaterWorld版 - 跟中文系的康蕾谈谈文学

http://bbs.tianya.cn/post-funinfo-4023670-12.shtml
摘录如下。可惜了,好歹也算当过我徒弟了。。。
附录2:吐了大妈的简历
Haiying Zhou's Education
postdoc at University of California at Berkeley
San Francisco Bay Area
Research
Haiying Zhou's Education
The University of Texas Health Science Center at San Antonio
Ph.D., Molecular Medicine
2001 – 2008
Nankai University
B.S, Chemistry
1993 – 1997
Haiying Zhou's Publications
Dual functions of TAF7L in adipocyte differentiation
eLIFE
January 8, 2013
Authors: Haiying Zhou
The diverse tr... 阅读全帖
W*******n
发帖数: 4140
29
来自主题: WaterWorld版 - 我的2011年版找工简历
我的2011年版找工简历
王利民
2014年8月16日
(现注:当时,未列出2010年在Fox Chase Cancer Center的经历;没列出2005年在
Memorial Sloan-Kettering Cancer Center的经历;没列出2006年在陕西师范大学、
2009年在江南大学任教的经历。)
Career Summary:
PhD degree on Chemistry and post-doctoral research on protein
structure in the USA; Teaching (assistance) experience in the USA and
China; Customer service experience in the USA.
Work/ Research:
Jan 2006 - : various work in the USA, China, and home.
Sept. 2003- June 2005: New York State Department of Health
Post-doctorate
... 阅读全帖
b****g
发帖数: 1204
30
Honor:
Highest score in the 1991' National College Entrance Exam in Hunan
Province, China
2011年版找工简历
王利民
Career Summary:
PhD degree on Chemistry and post-doctoral research on protein
structure in the USA; Teaching (assistance) experience in the USA and
China; Customer service experience in the USA.
Work/ Research:
Jan 2006 - : various work in the USA, China, and home.
Sept. 2003- June 2005: New York State Department of Health
Post-doctorate
Research projects: Crystal structure studies on superan... 阅读全帖
W*******n
发帖数: 4140
31
老年痴呆症赞比buydig,你何故不全文转发我的原贴呢?我全文再贴一下:
我的2011年版找工简历
王利民
2014年8月16日
(现注:当时,未列出2010年在Fox Chase Cancer Center的经历;没列出2005年在
Memorial Sloan-Kettering Cancer Center的经历;没列出2006年在陕西师范大学、
2009年在江南大学生物工程学院任教的经历。)
Career Summary:
PhD degree on Chemistry and post-doctoral research on protein
structure in the USA; Teaching (assistance) experience in the USA and
China; Customer service experience in the USA.
Work/ Research:
Jan 2006 - : various work in the USA, China, and home.
Sept. 2003- June 2005: New York... 阅读全帖
x**i
发帖数: 2627
32
刷屏挺好。。。

Honor:
Highest score in the 1991' National College Entrance Exam in Hunan
Province, China
2011年版找工简历
王利民
Career Summary:
PhD degree on Chemistry and post-doctoral research on protein
structure in the USA; Teaching (assistance) experience in the USA and
China; Customer service experience in the USA.
Work/ Research:
Jan 2006 - : various work in the USA, China, and home.
Sept. 2003- June 2005: New York State Department of Health
Post-doctorate
Research projects: Crystal structure studies o... 阅读全帖
b****g
发帖数: 1204
33
【 以下文字转载自 WaterWorld 讨论区 】
发信人: buydig (digbuy), 信区: WaterWorld
标 题: 这王利民是混的最惨的高考状元吗?
发信站: BBS 未名空间站 (Tue Aug 19 22:40:39 2014, 美东)
Honor:
Highest score in the 1991' National College Entrance Exam in Hunan
Province, China
2011年版找工简历
王利民
Career Summary:
PhD degree on Chemistry and post-doctoral research on protein
structure in the USA; Teaching (assistance) experience in the USA and
China; Customer service experience in the USA.
Work/ Research:
Jan 2006 - : various work in the USA, China, and ho... 阅读全帖
j****i
发帖数: 68152
34
【 以下文字转载自 WaterWorld 讨论区 】
发信人: buydig (digbuy), 信区: WaterWorld
标 题: 这王利民是混的最惨的高考状元吗?
发信站: BBS 未名空间站 (Tue Aug 19 22:40:39 2014, 美东)
Honor:
Highest score in the 1991' National College Entrance Exam in Hunan
Province, China
2011年版找工简历
王利民
Career Summary:
PhD degree on Chemistry and post-doctoral research on protein
structure in the USA; Teaching (assistance) experience in the USA and
China; Customer service experience in the USA.
Work/ Research:
Jan 2006 - : various work in the USA, China, and ho... 阅读全帖
D*****r
发帖数: 6791
35
来自主题: TrustInJesus版 - 查经贴-使徒行传
Acts 21:17-26 Paul’s Arrival at Jerusalem
17When we arrived at Jerusalem, the brothers received us warmly. 18The next
day Paul and the rest of us went to see James, and all the elders were
present. 19Paul greeted them and reported in detail what God had done among
the Gentiles through his ministry.
20When they heard this, they praised God. Then they said to Paul: “You see,
brother, how many thousands of Jews have believed, and all of them are
zealous for the law. 21They have been informed that y... 阅读全帖
J*****3
发帖数: 4298
36
定义:
简单地说就是按照自身的逻辑推演的话,自己可以证明自己至少不是矛盾或者错误的。
这就是简单的自洽性。科学研究本身就是遵循自洽性的,一个不能够满足自洽性的理论
或者方法显然是不攻自破的。
圣经自洽吗?很多自相矛盾的经文
GE 1:3-5 On the first day, God created light, then separated light and
darkness.
GE 1:14-19 The sun (which separates night and day) wasn't created until the
fourth day.
GE 1:11-12, 26-27 Trees were created before man was created.
GE 2:4-9 Man was created before trees were created.
GE 1:20-21, 26-27 Birds were created before man was created.
GE 2:7, 19 Man was created before birds were ... 阅读全帖
m****s
发帖数: 18160
37
来自主题: TrustInJesus版 - [合集] 查经贴-使徒行传
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Dramaer (不要问我从那里来) 于 (Fri Oct 22 12:02:52 2010, 美东) 提到:
Act 1:1-11 Jesus Taken Up Into Heaven
1In my former book, Theophilus, I wrote about all that Jesus began to do and
to teach 2until the day he was taken up to heaven, after giving
instructions through the Holy Spirit to the apostles he had chosen. 3After
his suffering, he showed himself to these men and gave many convincing
proofs that he was alive. He appeared to them over a period of forty days
... 阅读全帖
l****u
发帖数: 2166
38
Master Lu Tzu said: Since when has the expression "circulation of the Light"
been revealed? It was revealed by the "true men of the beginning of form".
When the Light is allowed to move in a circle, all the powers of Heaven and
earth, of the light and the dark, are crystallized. That is what is
described as seed-like, or purification of the power, or purification of the
concept. When one begins to apply this magic, it is as if, in the middle of
one's being, there was a non-being. When in the cou
f*******c
发帖数: 94
39
what sexpression system, and what section of gelatin, purification method?
If purification depends on traditional precipitation, this gelatin is trash
even it is recombinant human gelatin.
As I know, there are not successful gelatin (recombinant) produced in
industrial for several problems:
1) post-translation modification, which cause allerge and other immuno
problem.
2) Gelatin is a big and complex protein, which means no way or too difficult
to express whole gelatin in pichia or E Coli or oth
l****o
发帖数: 32
40
来自主题: ECUST版 - Amgen internship opportunity
Please apply for it online ASAP if you are interested in this opportunity
and I will recieve your resume right after that. Thanks.
Auto req 30057BR
Job Posting Title Grad Intern - Operations
Career Category College Job
Employee Subgroup Seasonal FT
Country (State/Region) United States - California
Location (City) US - CA - Thousand Oaks
Amgen Job Description Amgen’s 10 – 12 week internship program offers
meaningful project experiences that impact patient’s lives. This... 阅读全帖
r******m
发帖数: 173
41
来自主题: Biology版 - cloning菜鸟求教大家
I am trying to clone a 300 bp gene into pTYB2 vector (7.4kb)for later
protein purification method by IMPACT-CN. NdeI (producing sticky end) and
SmaI (blunt end) restriction sites were constructed into primers
corresponding to the N- and C- termini of the gene and incorporated by PCR
amplification. The PCR product and the vector were both double digested with
SmaI and NdeI respectively. After gel purification, the digested insert (
gene) and the vector were used to perform ligation with T4 DNA li
X***h
发帖数: 74
42
来自主题: Biology版 - 有一个digestion的问题
如果我PCR后的product比较干净,能否不处理直接restriction digest? 现在我比较麻
烦的是如
果用column purification总是丢失大部分的DNA,后面digest完了还要gel
purification,
那就所剩无几了。
或者用SAP 去除primers,然后Ethanol-sodium acetate precipitation,总可以上
digestion了吧。
s******r
发帖数: 2876
43
来自主题: Biology版 - 有一个digestion的问题
你既然都P出来了,完全可以用PCR产物第二次扩增一遍,
DNA还不是要多少有多少。

如果我PCR后的product比较干净,能否不处理直接restriction digest? 现在我比较麻
烦的是如
果用column purification总是丢失大部分的DNA,后面digest完了还要gel
purification,
那就所剩无几了。
或者用SAP 去除primers,然后Ethanol-sodium acetate precipitation,总可以上
digestion了吧。
a********k
发帖数: 2273
44
Gel purification下限是70吧,PCR purification kit下限100,nucleotide removal
kit下限40。
胶回收短片段可以用QG buffer溶胶,加isopropanol,再用nucleotide removal的柱子
就行了。反正三个柱子都是通用 的。
c***y
发帖数: 615
45
来自主题: Biology版 - DNA biotin label
This is what I have been thinking.
Is biotin at the end affecting the PCR efficiency and the purification
procedure afterward? Are PCR and PCR purification affecting the biotin's
existence at the DNA end?
s******y
发帖数: 28562
46
Depending on your purification methods, but I recommend the
RNase-free DNase kit from Qiagne.
It is CRITICAL to remove the Plasmid DNA, otherwise you are just doing PCR
on your plasmid, not the cDNA. Most RNA purification kit are designed to
remove genomic DNA but they are NOT designed to remove plasmid DNA,
therefore you have to do it yourself.
The reason you don't see the S16 can be due to:
1. you don't add enough cDNA template (plasmid DNA has strong asorption and
will mislead you to think yo
a****k
发帖数: 1130
47
How to dissect autophagy with biochemical purification approach?? I think
the biggest problem for this field is that they don't have good assays to
measure autophagy. The requirement of an assay which is good enough for
biochemical purification is even higher.
a****k
发帖数: 1130
48
RNAi screen是没问题的,但是用做purification是不可能的。能用做purification的assay本身必须reliable, rapid还要sensitive,而且从in vivo的现象到in vitro真正可以用来test column fractionation还是有巨大差距的啊
当然,你说的晓东这些例子肯定是没问题的啊,因为最终的目的就是要把一个生物现象简化到一些biochemical reaction嘛
a***e
发帖数: 1010
49
来自主题: Biology版 - siRNA做后用Trizol提取RNA
I agree that it may be not that important to treat RNA sample with DNaseI
during the RNA purification process in the original question. However, it is
better to maintain a "good habit" to do so.
There are several reasons for this:
(1) it doesn't add many extra work. DNaseI treatment is very simple and fast
. Qiagen has on-column DNaseI digestion kit. Or an extra TRIzol purification
step only adds one more hour.
(2) if the experiments include transfection of plasmid to overexpress some
genes or s
b*******e
发帖数: 4
50
打算定个试剂盒提去植物的microRNA,但定的时候没仔细看,等货到了发现说明上只是
说用于提取动物或人细胞或组织的microRNA,没说能不能用于植物的。
请版上各位高人帮俺看看这个试剂盒能用于植物么?要是不行也不知道能不能退啊,又
要被老板骂了。。。。哭。。。。
miRNeasy Mini Kit
For purification of microRNA and total RNA from tissues and cells
Effective purification of miRNA and total RNA
Efficient enrichment of miRNA and RNAs <200 nucleotides
Cat. no. 217004
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