H****s 发帖数: 301 | 1 往细菌里转了一个基因文库,发现了一个表型改变。似乎这个表型变化是不可逆的,挠
头中。我更感兴趣的是细菌的这个新表型。请问有没有什么办法去掉细菌里转进去的质
粒?这个质粒是基于pUC Ori的,是个高拷贝质粒。如果我在没有抗性的培养液中连续
传代,会不会彻底除去?谢谢。 |
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s*****i 发帖数: 5548 | 2 ☆─────────────────────────────────────☆
flow6666 (Lovegold) 于 (Wed Feb 13 12:46:13 2013, 美东) 提到:
夲人用该公司已一年多.去年七月renew它的12个月Fixed Plan, 9.2 cent per Kwh(
Terrific 12 plan). 如每月用电少于1000 kwh,付$6.95月费. 在Power to Choose上,
它家仍有Terrific 12 plan, 标着11 cents per Kwh. 但实际情况是, 你一旦加入, 你
的账单会你看不懂. 它不是显示你的用电数乘rate, 加上月费,稅等. 而是把rate降下
来(我的从9.2 cents 降到5.2), 但另外多出一项Centerpoint Energy Fees(有时写TDU
fee),该项费你不知多少,月月不同,可$18-45(我的case). 所以真正的rate变成了
15cents kwh以上. 如夲月我用了249 kw, 付了$42. BOUNCE ENERGY的策略是, 用小恩
小惠诱人... 阅读全帖 |
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t****h 发帖数: 185 | 3 南美面积有中国加美国一起那么大,私立大学众多.我说的薪水是排名靠前的私校的。
下面是QS的南美大学综合排名供参考。
1 100 Pontificia Universidad Católica de Chile Chile
2 98.9 Universidade de São Paulo (USP)Brazil
3 97.1 Universidade Estadual de Campinas Brazil
4 95.5 Universidade Federal do Rio de Janeiro Brazil
5 94.7 Universidad de Los Andes Colombia Colombia
6 94.1 Universidad de Chile Chile
7 91.4 Tecnológico de Monterrey (ITESM) Mexico
8 91 Universidad Nacional Autónoma de Méx... 阅读全帖 |
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p*****p 发帖数: 19331 | 4 他的bill是这样的
Energy Charges
Base Charge............................................... $4.95
Energy Charge............................ 741.00 @ 0.09200 $68.17
Taxes City Tax............................................ $1.12
PUC Assessment Charge..................................... $0.12
Gross Receipt............................................. $1.46
Total Current Charges..................................... $75.82
乱七八糟的费用包括Taxes City Tax,PUC Assessment Charge,Gross Receipt,2块多 |
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p*****p 发帖数: 19331 | 5 他的bill是这样的
Energy Charges
Base Charge............................................... $4.95
Energy Charge............................ 741.00 @ 0.09200 $68.17
Taxes City Tax............................................ $1.12
PUC Assessment Charge..................................... $0.12
Gross Receipt............................................. $1.46
Total Current Charges..................................... $75.82
乱七八糟的费用包括Taxes City Tax,PUC Assessment Charge,Gross Receipt,2块多 |
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发帖数: 1 | 7 12月 谈谈间谍问题
我一直感觉Reuters 有日本的间谍,他们会通过报道来传递针对华人屠杀的计划,和发
布不利于华人的报道,制造舆论。最重要他们后边的势力和犯罪集团有关。有必要好好
调查这个组织。
How top U.S. colleges hooked up with controversial Chinese companies
By Steve Stecklow and Alexandra Harney
Filed Dec. 2, 2016, noon GMT
New Oriental, China’s biggest private educator, has been accused of
academic fraud. Thanks to two enterprising Americans, it has also gained
access to leading U.S. college admissions officers.
SHANGHAI/SHELTER ISLAND, New York - Thomas Benson once ran a small... 阅读全帖 |
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发帖数: 1 | 8 直接从德国人嘴里抢食,默克尔能咽得下这口气吗?
General Electric Co. (GE - Get Report) rose on Wednesday, Oct. 17, after
reportedly beating out rival Siemens AG (SIEGY) for a multi-billion-dollar
deal to supply power to Iraq.
Munich-based Siemens had been close to securing a contract to supply 11
gigawatts of power-generation equipment to Iraq, a deal worth about $15
billion, but then the Trump administration intervened on behalf of GE, the
Financial Times reported, citing unnamed people familiar with the matter.
The ... 阅读全帖 |
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d******5 发帖数: 4273 | 12 原因有二:
1、副作用是个大问题。
2、来自AZN的药物与其竞争。
参见:
http://www.thestreet.com/story/12732278/1/why-clovis-
oncology-clvs-stock-hit-a-one-year-low-today.html?
puc=yahoo&cm_ven=YAHOO
NEW YORK (TheStreet) -- Clovis Oncology (CLVS_)
continued to plunge to a one-year low of $38.48 on
Tuesday with another day of double-digit declines
following disappointing data from ASCO 2014 with
regard to the company's investigational non-small cell
lung cancer drug, CO-1686.
The drug, designed to target target T790M-positive
mut... 阅读全帖 |
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s******o 发帖数: 45 | 24 State接管,成本会非常高。美国的大部分utility都是官督商办的模式,你让政府亲自
来run这些units和retail branch,先看看成本再说。
另外你要搞清楚,utility要从supplier那里买电买fuel。如果一个公司快Chapter 11
了,是没有supplier愿意跟他做transaction的。在山火刚发生的时候,股票闪崩了一
回,当时很多的supplier立马freeze credit with PG&E, 所以才有后来的PUC出来喊话。
这个PG&E的case,根源在于Utility是一个regulated business. 加州的法律给utility
增加了很多的burden。看好这会是一次有意思的政治博弈。
另外,官司缠身的公司,价格低到一定程度,机会就出来了。看你自己怎么理解这个
game了。 |
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e*****a 发帖数: 95 | 25 看看这篇,就知道他是不是虚伪了。
https://thinkprogress.org/buffett-says-he-loves-renewables-so-why-is-his-
company-trying-to-kill-solar-energy-77a6941bafc1#.19dvf1r7u
Yet his solar-hyping shareholder letter never mentions that BHE owns NV
Energy, which almost single-handedly destroyed the exploding solar rooftop
market in Nevada. As we reported last year, Buffett’s utility successfully
lobbied the Nevada Public Utilities Commission (PUC) to slash the “net
metering” payments to solar customers for the electricit... 阅读全帖 |
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z*******g 发帖数: 94 | 26 即将来到普度大学的新同学们:
大家好!
首先恭喜大家拿到普度大学的录取通知。普度大学历史悠久,是美国久负盛名的传统
名校。她的校友遍布世界各地。抗日名将孙立人、“两弹元勋”邓稼先,登上月球的第
一人阿姆斯特朗等人,都曾在这里就读。今天,普度大学仍是在许多领域领先的综合性
研究大学,也是美国招收国际学生最多的学校之一。
普度大学中国学生学者联谊会(Purdue University Chinese Students-Scholars
Association, PUCSSA)自一九九一年成立以来,已经走过了十九个春秋。虽然其间人
员更迭,但是我们始终致力于更好的服务本地中国同胞,并为宣传中国文化而不懈努力。
作为本届联谊会的执行委员会(Executive Committee)成员,我们也都曾经是“新
生”,曾经走过和你们一样的道路。我们非常理解同学们即将踏上一片陌生的土地并开
始新的生活时的感受。我们更愿意尽我们所能帮助大家安全顺利地到达学校,帮助你们
解决生活学习上的困难。我们写的这封信,主要是简要介绍一下联谊会在新生接待上的
准备工作。
这次的新生接待工作由PUC |
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W***n 发帖数: 11530 | 29 A loophole in immigration law is costing thousands of American jobs
Michael Hiltzik
Los Angeles Timesmichael.hiltzik@latimes.com
@hiltzikmA
loophole in immigration law is costing thousands of American jobs--and
Congress wants to expand it: http://t.co/y3y6upmDMt
Edison cuts hundreds of tech jobs
Since last summer, Edison has been firing domestic IT workers, replacing
them with outsourced ones from India
'We can get four Indian guys for cheaper than the price of you,' laid-off
Edison worke... 阅读全帖 |
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p***q 发帖数: 515 | 31 可以投诉啊。到PUC和BBB投诉比你在论坛上贴效果好多了。 |
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s**********n 发帖数: 2343 | 33 In 1999, an issue was raised of possible contamination of a portion of the
Western Area by
radionuclides during the period when the Western Area was used in part for
evaporation
ponds and a sludge drying bed associated with the former Pleasanton
Wastewater
Treatment Plan (PWTP). Such contamination could theoretically have occurred
as a
consequence of discharge of wastewater that might have subject to
contamination with
substances originating from the Lawrence Livermore Laboratory, through the
l... 阅读全帖 |
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c*****r 发帖数: 8227 | 34 看看有多少政府机构就很清楚了:
California Academic Performance Index (API) * California Access for Infants
and Mothers * California Acupuncture Board * California Administrative
Office of the Courts * California Adoptions Branch * California African
American Museum * California Agricultural Export Program * California
Agricultural Labor Relations Board * California Agricultural Statistics
Service * California Air Resources Board (CARB) * California Allocation
Board * California Alternative Energy and Advanced ... 阅读全帖 |
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S*******h 发帖数: 7021 | 37 看看这篇,就知道他是不是虚伪了。
https://thinkprogress.org/buffett-says-he-loves-renewables-so-why-is-his-
company-trying-to-kill-solar-energy-77a6941bafc1
Yet his solar-hyping shareholder letter never mentions that BHE owns NV
Energy, which almost single-handedly destroyed the exploding solar rooftop
market in Nevada. As we reported last year, Buffett’s utility successfully
lobbied the Nevada Public Utilities Commission (PUC) to slash the “net
metering” payments to solar customers for the electricity they put ... 阅读全帖 |
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f******6 发帖数: 72 | 38 I don't think Bounce Energy is good choice!!! They are not honest.
1.I enrolled in their month-to-month plan on 4/15/11(rate 6 cent/kwh).They
only gave me that rate for for 6 days. Then raised the rate to 11.4 cent for
this plan(they will not show this in their website). I called them to
swicth to 12 month fixed plan(9.2 cent) in 5/10. My recent statement still
showed 11.4 cent per kwh.They said I have to use 11.4 cent for one cycle.
2.They have many Misc Fees which I didn't see in several oth... 阅读全帖 |
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g*r 发帖数: 2116 | 39 贵了点。。。
给你看看我的
Current Meter Read 07/13/2012 19,561
Previous Meter Read 06/13/2012 18,291
Days in Cycle 30
Electricity Plan: Terrific 12 @ 5.1288 cent/kWh
Cost of 1270 kWh of Usage: $65.14
Base Charge: $0.00
TDU Fees: $52.55
PUC Assessment: $0.18
Gross Receipts Reimb: $2.16
Other Charges and Credits
Charges for this Meter and Cycle: $120.03
Sales Tax: $1.10
Total Current Monthly Charges: $121.13
Previous Balance: $0.00
Payments: $0.00
Current Charges Due: $121.13
The average price you paid for el... 阅读全帖 |
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g*****e 发帖数: 641 | 40 我觉得你是没有搞清楚centerpoint费用是电费的一部分吧,其他家其实不显示这个费
用但是也包含在电价里面了
我看很多说bounce黑的估计没选fix的plan 所以价格波动也是难免了,fix那个价格是
看The average price you paid,我当时定的时候是9c一度, 如果用电没有低于1000
度是没有base charge的,那么每次就是9c一度(这个包含了centerpoint的费用了)如
果低于1000,会平均价格会略高一点
贴我家的bounce上个月电费单
Current Meter Read 10/03/2013 24870
Previous Meter Read 09/04/2013 23687
Days in Cycle 29
Electricity Plan: Fantastic Fixed 6 @ 4.9024 cent/kWh
Cost of 1183 kWh of Usage: $58.00
Base Charge: $0.00
The average price you paid for electric service this mont... 阅读全帖 |
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f******6 发帖数: 72 | 41 账单一部分,啍,让你看得头晕!
The average price you paid for electric service this month:15.3 cents/kWh
Centerpoint Energy Fees: $18.13
PUC Assessment: $0.06
The contract term of your plan ends on 7/9/2013
Centerpoint Energy Fees Include Charges For:
Transmission Cost Recovery Factor - $0.28
Transmission System Charge - $2.10
Rate Case Expenses Surcharge - $0.01
Energy Efficiency Cost Recovery Factor - $0.93
Advanced Metering Charge - $3.05
Distribution System Charge - $4.11
ADFIT Credit - $-0.09
Metering Cha... 阅读全帖 |
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s*******1 发帖数: 3820 | 42 Electricity Plan: Tremendous 24 @ 10.1660 cent/kWh
Cost of 398 kWh of Usage: $40.46
Base Charge: $4.95
The average price you paid for electric service this month:12.4 cents/kWh
Centerpoint Energy Fees: $3.98
PUC Assessment: $0.09
Gross Receipts Reimb: $0.00
Other Charges and Credits
Late Payment Penalty $1.83
Charges for this Meter and Cycle: $51.31
Sales Tax: $0.00
Total Current Monthly Charges: $51.31
Centerpoint Energy Fees都要收的吧?根据用量算的 |
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J**X 发帖数: 2433 | 43 Current Charges
Monthly Customer Fee $0.00
Advanced Meter $2.19
Energy Charge (254 x $0.07500 per kWh) $19.05
Miscellaneous Gross Receipts Tax Reimbursement $0.42
PUC Assessment $0.03
Sales Tax $0.21
Total Current Charges $21.90
Total Amount Due $21.90
The average price you paid for electric service this month was: $0.084
我上个月的帐单,做饭热水取暖都是烧气,又经常出门,
所以用电很少,但用的多单价应该更低。
这是个叫summer energy的小公司,我以前提过一次,好像没人感兴趣,
弄得我心里还没底,以为有什么猫腻,但用了几个月一直是这个价,
希望过几个月能续上差不多的价钱。 |
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l**t 发帖数: 452 | 44 不知道有多少华人在power wholesale, retail, trading这些领域工作。我了解一些皮
毛,觉得里面的水太深了。
首先监管非常严密,有无数的报告要向FERC和PUC汇报,比SEC有过之而无不及。
其次风险也很大,各种derivatives在电力交易市场得到充分运用,稍微不会注意就会
赔钱。
第三,当然,收益也很大。
before
谱? |
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w****r 发帖数: 15252 | 45 Bounce的,用的比少,账单还比你多,黑吧
Current Meter Read 09/10/2013 27955
Previous Meter Read 08/09/2013 27285
Days in Cycle 32
Electricity Plan: Custom Plan 3 @ 6.9036 cent/kWh
Cost of 670 kWh of Usage: $46.25
Base Charge: $6.95
The average price you paid for electric service this month:12.8 cents/kWh
Centerpoint Energy Fees: $32.10
PUC Assessment: $0.13
Gross Receipts Reimb: $1.61
Other Charges and Credits
Charges for this Meter and Cycle: $87.04
块多 |
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g*****e 发帖数: 641 | 46 我觉得你是没有搞清楚centerpoint费用是电费的一部分吧,其他家其实不显示这个费
用但是也包含在电价里面了
我看很多说bounce黑的估计没选fix的plan 所以价格波动也是难免了,fix那个价格是
看The average price you paid,我当时定的时候是9c一度, 如果用电没有低于1000
度是没有base charge的,那么每次就是9c一度(这个包含了centerpoint的费用了)如
果低于1000,会平均价格会略高一点
贴我家的bounce上个月电费单
Current Meter Read 10/03/2013 24870
Previous Meter Read 09/04/2013 23687
Days in Cycle 29
Electricity Plan: Fantastic Fixed 6 @ 4.9024 cent/kWh
Cost of 1183 kWh of Usage: $58.00
Base Charge: $0.00
The average price you paid for electric service this mont... 阅读全帖 |
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w****r 发帖数: 15252 | 47 Bounce的,用的比少,账单还比你多,黑吧
Current Meter Read 09/10/2013 27955
Previous Meter Read 08/09/2013 27285
Days in Cycle 32
Electricity Plan: Custom Plan 3 @ 6.9036 cent/kWh
Cost of 670 kWh of Usage: $46.25
Base Charge: $6.95
The average price you paid for electric service this month:12.8 cents/kWh
Centerpoint Energy Fees: $32.10
PUC Assessment: $0.13
Gross Receipts Reimb: $1.61
Other Charges and Credits
Charges for this Meter and Cycle: $87.04
块多 |
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g*****e 发帖数: 641 | 48 我觉得你是没有搞清楚centerpoint费用是电费的一部分吧,其他家其实不显示这个费
用但是也包含在电价里面了
我看很多说bounce黑的估计没选fix的plan 所以价格波动也是难免了,fix那个价格是
看The average price you paid,我当时定的时候是9c一度, 如果用电没有低于1000
度是没有base charge的,那么每次就是9c一度(这个包含了centerpoint的费用了)如
果低于1000,会平均价格会略高一点
贴我家的bounce上个月电费单
Current Meter Read 10/03/2013 24870
Previous Meter Read 09/04/2013 23687
Days in Cycle 29
Electricity Plan: Fantastic Fixed 6 @ 4.9024 cent/kWh
Cost of 1183 kWh of Usage: $58.00
Base Charge: $0.00
The average price you paid for electric service this mont... 阅读全帖 |
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p*****p 发帖数: 19331 | 49 新bill
不用冷气,少了好多啊
整体算下来,单价不到11分,虽然只用了500多度
Current Charges
Energy Charges
Base Charge............................... $4.95
Energy Charge............. 516.00 0.09200 $47.47
Taxes
City Tax................................. $0.80
PUC Assessment Charge..................... $0.09
Gross Receipt.............................. $1.05
Total Current Charges...................... $54.36 |
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r*****t 发帖数: 7278 | 50 Energy Charges
Base Charge.................................................................
.... $4.95
Energy Charge...............................................................
... 148.00 0.09203 $13.62
Taxes
City Tax....................................................................
......... $0.19
PUC Assessment Charge................................................. $0.03
Gross Receipt...............................................................
.... $0.37
Total Current Charges............ 阅读全帖 |
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