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全部话题 - 话题: primary
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s********1
发帖数: 581
1
primary care provider在医院兼职重要吗?
将病人refer到该医院会更容易吗?
thanks
s********1
发帖数: 581
2
来自主题: PKU版 - 如何选择primary care provider?
如何选择primary care provider?
从yelp和healthgrades只能看到几个review,而且评价各不相同.怎么才能了解更多的
情况呢?
谢谢!
j**********t
发帖数: 3480
3
来自主题: PKU版 - 如何选择primary care provider?
primary care的差别不大,是我就选便宜离家近的,善解人意的医生有加分!有语言方
面的顾虑的话也可以考虑这个因素。我原来在的大城市里通常第一个appointment是医
生病人互相interview,任何一个人看不对眼就bye bye。
s********1
发帖数: 581
4
primary care provider在医院兼职重要吗?
将病人refer到该医院会更容易吗?
thanks
j**********t
发帖数: 3480
5
对于我来说这些问题都无关紧要。我找医生最多给自己做个年检和一些很简单的处方。
加拿大是全民公费医疗,只有大病才住院。需要住院的话处理得都很快,各个单位都会
尽量开绿灯,有没有primary care provider都无所谓。
p****1
发帖数: 275
6
来自主题: UMD版 - 请推荐 Primary Care Doctor
请推荐一个好的Primary Care Doctor,female preferred.
Thanks.
y***g
发帖数: 1
7
来自主题: Database版 - primary key?
得到primary key的column name的sql指令是什么?
要到用的时候忽然想不起来了。
y****r
发帖数: 1
8
来自主题: Database版 - how to get primary key through JDBC?
Hi, I forgot how to get primary key through JDBC. Is there anyone can do
me a favor?
Thanks in advance!
p*****e
发帖数: 58
9
来自主题: Database版 - multicolumn primary key ?
sure. In the design view while holding the Ctrl key click
the columns you will select multiple columbs. Write click
and choose "Primary key"
s***x
发帖数: 34
10
来自主题: Database版 - How to find primary key
An urgent problem.
Could anyone tell me how to find the primary key of a table defined
by someone else? (in PostgreSQL)
Thanks.
d********r
发帖数: 199
11
一个表,我有两个列(比如:c1, c2)联合起来作 Primary Key,
如果我还有这样的search 需求:
select ... where c1=...

select ... where c2=...
在这种情况下,如果要提高速度的话,
还需要对这两个column分别建index吗?
I*****y
发帖数: 6402
12
来自主题: Database版 - indexing就是设置primary key吗?
在建mysql数据库table的时候,因为read这个表格多于insert, 想弄一个index, 根据我
现在的理解就是设置primary key, 请问这样理解对吗?
j*****n
发帖数: 1781
13
来自主题: Database版 - indexing就是设置primary key吗?
not sure for mysql, but for MSSQL, if you specify a primary key, it will
automatically create a clustered index for you.
anyway, indexing is different thing with your PK...
get your knowledge updated by read some database fundamental books please.
m******1
发帖数: 98
14
MS ACCESS 里面怎么把两个Table combine 到一个table, 而且primary key不重复呢?
Query可以把两个并到一起,但是我想直接把他们并成一个table.而且ID 不重复。
不知道怎么弄,请教。 谢谢。
l******9
发帖数: 579
15
I need to create a unique primary key that can also be indexed in IBM
netezza sql server ?
I find that netezza does not support UNIQUE key.
Are there some ways to get around this problem ?
Thanks
s**********o
发帖数: 14359
16
大哥概念没学好吧
PRIMARY KEY = CLUSTERED INDEX+UNIQUE
z**********8
发帖数: 2049
17
我需要import data 从一个excel file 到一个database 的table, 该table中有一个
column, “donationID”(primary key) 但是被设置成read only. 所以,导致我用
import wizard 无法输入。
我必须跟dba讲,改掉设置吗?
不知道问题讲清楚没有。
d******8
发帖数: 2191
18
从包含primary key的数据库里读取数据保存到了DataSet,使用DataSet.
DataTableCollection[0].NewRow创建新的DataRow,查了下pk column,发现value从0
开始。需要手动置下AutoIncrementSeed。
之前没这个问题,求大牛指点下可能的原因所在。
a****m
发帖数: 693
19
#include
#include "GCD.hpp"
using namespace std;
class CFraction
{
public: //private method to get GCD
int iNumerator;
int iDenominator;
//constructor...
CFraction( int iNumeratorParam, int iDenominatorParam )
{
iNumerator = iNumeratorParam;
iDenominator = iDenominatorParam;
}
//getGCD
int getGCD( int iNumerator, int iDenominator )
{
int remainder = iDenominator % iNumerator;
if ( remainder != 0 )
return getGCD( remainder,iNumerator );
else return 0;
}
CFraction reduce()
{
int iGC... 阅读全帖
a****m
发帖数: 693
20
CFraction reduce()
{
int iGCD = getGCD(int iNumerator, int iDenominator);
// 总是在这一行出问题: expected primary-expression before "int"
//It seems that it does not need redefine the type, getGCD(inum, idenom)
iNumerator=iNumerator/iGCD;
iDenominator=iDenominator/iGCD;
}
thanks all you guys!
d*********e
发帖数: 3835
21
来自主题: Aviation版 - Primary Account Manager
CA Global: Africa Positions has a client that is in need of a Primary
Account Manager. The position is based in Abu Dhabi.
Overview:
Commercial:
• To evaluate the company’s market position in the defined region
• To suggest modifications to the short term sales objectives in line
with the market situation
• To anticipate market trends and changes
Account Management:
• To maintain contact with customers
• To report on sales activities
• To keep abreast of regio... 阅读全帖
s******y
发帖数: 28562
22
Do you mean the primary structure (protein sequence)?
Or the exact crystal structure? (3rd structure)?
Either way you can find them on NCBI database
y*******n
发帖数: 127
23
What I need the primary structure. I need to the location of groups such
amine,carboxy,thio....
A******y
发帖数: 2041
24
virus...using chemical agents for primary cells and lymphocytes don't expect
much. Now you know why big pharmas are dropping RNAi division.
y*********r
发帖数: 78
25
I had the same problem before with murine primary T cells. Probably
retrovirus is better.
w****u
发帖数: 1078
26
I tried retrovirus for OVER expression on murine primary T cells, there are
>60% infection. So, if lentiviral shRNA are not good for infection. Is that
better to clone this into retrovirus vector? thanks
q****2
发帖数: 208
27
primary mice B cell??
b*******0
发帖数: 53
28
想作Colony formation assay,请版上高人推荐哪儿可以买到primary rat embryo
fibroblast (REF) cells?
Thanks a lot!
n*******n
发帖数: 515
29
版上高手众多,如果知道答案,不吝赐教。
我是做有机合成的,平时也会养一些细胞, 主要是cancer cell lines。
这次被老板指派了一个project需要primary neuron cells。这可从来没做过,试了几
次,可每次harvest neuron cells用trypsin或是scraping的话要不然下不来,要不然
百分之六七十的细胞已经挂了。。。
请有经验的同学指点一下吧,有包子。谢谢
m*********n
发帖数: 215
30
你是把primary neuron种在什么表面上? falcon dish? coated with what?
还有你收集neuron后准备用来做什么?
h*******o
发帖数: 4884
31
Please provide more details on your experimental design.
Usually, you only collect/harvest primary neuronal cultures at the end step,
which means you have complete all the live cell assays.
h*******o
发帖数: 4884
32
For primary neurons, we use neurobasal media +B 27. It is selective towards
neurons and glia usually cannot survive for long term in NBM+B27. With that
said, there is no pure neuronal culture. Anyway, cultured neurons do exhibit
some glial properties, such as glucose transporter expression, metabolic
activity, etc.
For embryonic glial culture, we use DMEM/F12 (1:1) +10% FBS. Because glial
cells are mitotic whereas neurons are post-mitotic, neurons will be easily
outnumbered by proliferative glia... 阅读全帖
s****e
发帖数: 165
33
今天看到一个学校,Faculty的title是这样写的:Primary Faculty and Affiliated
Faculty。
请教知情者,这是什么title?是实验室的PI吗?
谢谢!!!
c****d
发帖数: 501
34
来自主题: Biology版 - 关于primary B cells的培养
版上有没有人是使用primary human B cell的?
目前的培养条件是什么呢
search了很多文献 可是没有一个清晰的共识的结论
w********r
发帖数: 1431
35
primary cell难道不是要现用么?
冻起来再传几代谁知道里面变成什么样子了...
f*****f
发帖数: 195
36
就实验研究手段而言,两者有啥区别?
satellite cell可以认为是muscle stem cell,我看到paper上大部分都是小鼠in vivo
的实验。primary myoblast实际上也是将satellite cell传代分离得到的。那么,假如
体外培养muscle stem cell,不就变成了myoblast?
谢谢!
z****u
发帖数: 1007
37
不是很明白你这个问题。
传统说法是Pax7+ satellite cell变成myogenic cells, proliferate.. primary
myogenesis
then secondary myogenesis, myoblast cluster and form the myotubes.
体外养satellite cells的方式好像很局限。我还没看到好的protocl能体外扩增
satellite cell without losing the undifferentiate status.

vivo
f*****f
发帖数: 195
38
谢谢。
分离primary myoblast 有很成熟的protocol
http://www.stanford.edu/group/blau/protocols/mouseprimarymyobla
体外没法保持quiescent状态,培养myoblast也可以增殖,那么这一个过程类似stem
cell的self-renewal?可以算你所说的undifferentiate status?
v***a
发帖数: 1242
39
最近一直做siRNA transfection做不出,找不出问题在哪。想换一个转染试剂。
没有用过主流的Lipofectamine系列,不知道哪一款比较适合primary mammalian Cells?
还看到Mirus Bio 的 TransIT-X2 广告,说“TransIT-X2 is an innovative non-
liposomal, polymeric system that has better transfection performance than
Lipofectamine 2000”,不知是真是假?
恳请高手推荐个好用的。
m****a
发帖数: 427
40
请问大家有养human primary endothelial cells from bone marrow and core blood
的经验吗?有的话,能不能share一下你们用的什么media和方法呀,谢谢啦
r******k
发帖数: 446
41
除了primary GBM ,Secondary GBM是不是已经是最高级的GBM?
k*****q
发帖数: 4
42
来自主题: Biology版 - CRISPR on primary immune cells?
请教大牛, 可以用CRISPR KO primary immune cells 吗?怎么做。 谢谢。

发帖数: 1
43
来自主题: Biology版 - CRISPR on primary immune cells?
try to use virus systems.
Method for Dual Viral Vector Mediated CRISPR-Cas9 Gene Disruption in Primary
Human Endothelial Cells
Haixia Gong, Menglin Liu, Jeff Klomp, Bradley J. Merrill, Jalees Rehman &
Asrar B. Malik
http://www.nature.com/articles/srep42127
e*****r
发帖数: 144
44
Often see this name in some tutorials.
Can somebody explain what is the purpose of the primary channel? And what is
it?
Thanks
j**d
发帖数: 36
45
来自主题: MedicalCareer版 - IM里面的primary care是什么?
IM中除了preliminary,categorical,还有primary care,我不太明白.
e*****a
发帖数: 1334
46
来自主题: MedicalCareer版 - IM里面的primary care是什么?
I think this is primary care track within IM. It focus on some aspects of an FM residency (without OB and Peds).
e*****a
发帖数: 1334
47
Do you mean IM-primary care track? If so, it's eligible for IM fellowships.
b*********r
发帖数: 39
48
来自主题: MedicalCareer版 - 关于internel medicine 的 primary care track
internal medicine 很多program 都会有primary care track, 哪位同学可以介绍介绍
这个track的特点, 以及喜欢招什么背景的candidate. 是不是对于research不是很在乎
? 多谢!
E*******9
发帖数: 152
49
来自主题: MedicalCareer版 - 关于internel medicine 的 primary care track
同问。
顶一顶。
some programs have primary care track (categorical); internal medicine (
categorical). could both be selected at the same time?
thanks for any comments!
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