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a*****g
发帖数: 543 | 2 Just my two cents:
1. 我不反对也不支持CSC的model.但是光从mutation的普遍性来说并不能否定CSC. 我
们现在还没法确定这些genetic heterogeneity是driver 还是passager.大多数只是ca
ncer的result而不是cause。Moreover, it is possible that CSC and Non-CSCs all
have genetic changes;remember the so-called CSCs don't have to follow the
biology of typical stem cells.
If cancer strictly follows evolution model, should one imagine certain gene
tic lesions gain dominance?
As for the genomic stability, why should we believe it is promoting tumor?
As far as I know,... 阅读全帖 |
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d***s
发帖数: 1062 | 3 我是一名医学院的新生，最近在选rotation的实验室。对一个实验室感兴趣，研究
development of pancreatic beta cells的,以斑马鱼为模型。
对斑马鱼这个模式生物不是很了解，以后还是想做尽量和医学疾病相关的研究。我们这
的医学院很少实验室是做斑马鱼的，其他的都是拿小鼠，大鼠做模型的。本人知识有限
，总感觉做斑马鱼太基础了。
想问问板上各位前辈的建议。谢谢 |
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s**u
发帖数: 2429 | 4 有这方面的专家么？查文献的结果好像很矛盾。一些说是endodermal, 一些说是
ectodermoral
(neural creast in origin). 现在有定论了么？ |
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n***3
发帖数: 663 | 5 Science. 2011 Mar 25;331(6024):1612-6.
CD40 agonists alter tumor stroma and show efficacy against pancreatic
carcinoma in mice and humans.
哪位好心人给发一下阿？supplementary information也帮忙一并下载。
please send the paper to e*********[email protected]
Thanks so much |
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C***k
发帖数: 1570 | 6 Endocr Relat Cancer. 2010 Aug 16;17(3):771-83. Print 2010 Sep.
MEN1 in pancreatic endocrine tumors: analysis of gene and protein status in
169 sporadic neoplasms reveals alterations in the vast majority of cases.
please send to: c**********[email protected]
3x very much!!! |
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c**l
发帖数: 319 | 7 Hyperlipidemia Causes Pancreatic Inflammation and Ductal Proliferation in
Apolipoprotein E Deficient Mice
Mallen-St. Clair, J.; Joaquin, V. A.; Fisher, E. A.; Bar-Sagi, D.
Pancreas, November 2007,35(4):414-415
请发pdf到g*****[email protected]
包子奉上, Thanks |
|
y******8
发帖数: 1764 | 8 Abbreviated Journal Title Impact Factor 5-Year Impact Factor
Immediacy Index Cited Half-Life Eigenfactor Score Article Influence
Score
CELL RES 8.151 5.65 1.962 2.8 0.02329 2.417
NANO RES 4.37 4.389 0.57 1.4 0.00262 2.332
FUNGAL DIVERS 3.803 2.669 1.184 2.7 0.00312 0.59
MOL PLANT 2.784 2.784 0.901 1.3 0.0024 1.308
CELL MOL IMMUNOL 2.765 0.316 57 0.00534
EPISODES 2.122 2.55 0 0... 阅读全帖 |
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D*****y
发帖数: 95 | 9 Glucose inhibits angiogenesis of isolated human pancreatic islets
J Mol Endocrinol 2010 45 99-105
请发到我邮箱 y***[email protected]
万分谢谢 |
|
y**u
发帖数: 7459 | 10 嗯，没说清楚, 是mouse pancreatic beta cells, insulin-secreting, 不是spleen那
个。 |
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c********r
发帖数: 1125 | 11
不止一篇pnas,一篇cell metabolism。。。
Zhou KM; Dong YM; Ge Q ... Xu T, PKA activation bypasses the requirement for
UNC-31 in the docking of dense core vesicles from C.elegans neurons, Neuron
, 2007, 56: 657-669.
Bai L, Wang Y, Fan JM... Xu T. Dissecting multiple steps of GLUT4
trafficking and identifying the sites of insulin action. Cell Metabolism.
2007, 5:47-57.
Kang LJ, He ZX, Xu PY, Fan J, Betz A, Brose N, Xu T. Munc13-1 is required
for the sustained release of insulin from pancreatic beta cells. C... 阅读全帖 |
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W*****o
发帖数: 1780 | 12 去查了一下，张旭总共发表了90多篇英文文章（不算中文）。回国之后作为联系作者也
有快20篇了。其中有cell, neuron, Journal of neuroscience, PNAS 好多篇。而且他
1995年就回国了，那个时候国内的环境有多差不用说了吧。这也快20年了。比饶益2007
年才全职回去多了12年。比起培养的中国学生也多多了，算国内贡献肯定不会小。如果
他一直留在国外，成就未必就比饶差。谁把饶益的文章列一列。国内多少篇，总共多少
篇，培养了多少中国学生？
1. Li KC, Wang F, Zhong YQ, Lu YJ, Wang Q, Zhang FX, Xiao HS, Bao L, Zhang X
. (2011) Reduction of follistatin-like 1 in primary afferent neurons
contributes to neuropathic pain hypersensitivity. Cell Res, 21: 697-699.
2. Li KC, Zhang FX, Li CL, Wang F, Yu MY, Z... 阅读全帖 |
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p****n
发帖数: 9263 | 13 pancreatic cancer 能拖这么久，千老们该很欣慰了
He |
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b*******0
发帖数: 125 | 14 是pancreatic cancer or liver prostration ？ |
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j*****d
发帖数: 787 | 15 “Although the broad outlines of Mr. Jobs’s struggle with pancreatic cancer
are known, the new biography, by Walter Isaacson, offers new insight and
details. Friends, family members and physicians spoke to Mr. Isaacson openly
about Mr. Jobs’s illness and his shifting strategy for managing it.
According to Mr. Isaacson, Mr. Jobs was one of 20 people in the world to
have all the genes of his cancer tumor and his normal DNA sequenced. The
price tag at the time: $100,000. ”
不知是否有过人之处.......
这家伙的确是属于“... 阅读全帖 |
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r**a
发帖数: 121 | 16 最近在做组织的FACS，用Trypsin和collagenase处理
我用的是10X Trypsin EDTA，后来发现EDTA是Trypsin和collagenase的抑制剂
Trypsin Inhibitors:
* Pancreatic-, soybean-, lima bean-, and egg white- trypsin inhibitors (
see section on Trypsin Inhibitors)
* DFP
* Aprotinin
* Ag+
* Benzamidine
* EDTA
Collagenase Inhibitors:
* EDTA, EGTA
* Cysteine, histidine
* DTT
* 2-mercaptoethanol
* o-phenanthroline
* Hg2+, Pb2+, Cd2+, Cu2+, Zn2+
* Not inhibited by DFP or serum
(White and White ... 阅读全帖 |
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a******7
发帖数: 7936 | 17 是啊，这我也知道
你看看他的原帖吧：
========================================
发信人: Edisonlee (超级赛亚人), 信区: WaterWorld
标 题: Re: 人体五脏六腑都重要，我单单不知道唾液有什么作用？
发信站: BBS 未名空间站 (Mon Dec 12 14:08:25 2011, 美东)
唾液淀粉酶很重要。。。消化淀粉大多靠唾液勒
==========================================
我就回了这个帖子一个 “错”，他就开始不好好说话了：
===========================================
发信人: Edisonlee (超级赛亚人), 信区: WaterWorld
标 题: Re: 人体五脏六腑都重要，我单单不知道唾液有什么作用？
发信站: BBS 未名空间站 (Tue Dec 13 15:45:29 2011, 美东)
不懂就别装。
哥学生物的。
快回高中补课去吧
========================================... 阅读全帖 |
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y********g
发帖数: 782 | 18 本人女生， 想去参加这个会。但是总共只有1600美金的额度，结果房间太贵$209啦，
想找个女生share。请站内联系~~~ |
|
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l**********1
发帖数: 5204 | 20 1992 til 2000
/iom.edu/~/media/Files/Activity%20Files/Disease/NCPF/Fund.pdf
2001 to 2009
//cancer.gov/researchandfunding/reports/pancreatic-research-progress.pdf
from 2008 to 2011 World R&D Summary report and 2011 to 2015 forecast
please go to
//www.battelle.org/aboutus/rd/2011.pdf |
|
w******y
发帖数: 2504 | 21 Immunol Cell Biol. 2011 Nov 8. doi: 10.1038/icb.2011.89. [Epub ahead of
print]
Macrophage migration inhibitory factor deficiency protects pancreatic islets
from palmitic acid-induced apoptosis.
Saksida T, Stosic-Grujicic S, Timotijevic G, Sandler S, Stojanovic I.
SourceDepartment of Immunology, Institute for Biological Research 'Sinisa
Stankovic', University of Belgrade, Belgrade, Serbia.
Could anyone help me send a copy mentioned above to my email: purplewindxxh@
hotmail.com. Thanks so much. |
|
h*******o
发帖数: 4884 | 22 有没有什么药物，刺激后能产生糖尿病
STZ-induced diabetes is a well accepted diabetic model, however, not sure
how suitable it is for your case.
STZ basically induces pancreatic islet damages.
The other model is high fat diet model.
有没有什么药物，刺激后能够加速新陈代谢
In vitro, yes a lot.
In vivo, not that I know of.
分析脂肪前体细胞的数量和功能(Gene A KO vs WT)
You got a very interesting mouse model.
If money is not an issue, I would quickly run a proteomic analyses in the
liver and probably the brain (since I work in neurosciences).
Also, s... 阅读全帖 |
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p**d
发帖数: 14 | 23 单抗不是小肽，150kDa左右,所以circulation time 要比小分子药物长很多，但是
delivery要差不少。(Rituximab的delivery问题要小些,因为lymphoma要比solid
tumors 更加accessible.)现在的单抗都是fully humanized, 不会有免疫反应。这个药
失败主要是因为pancreatic cancer太难治了,另外说明IGF1R 的singaling pathway不
是driver. |
|
A******y
发帖数: 2041 | 24 Pancreatic cancer is highly fibrous. Part of the reason that it is hard to
curve is that most drug can not make it to the target site. |
|
l**********1
发帖数: 5204 | 25 Sure
plus
Ku GM et al. (2012)
An siRNA screen in pancreatic beta cells reveals a role for Gpr27 in insulin
production.
PLoS Genet. 8: e1002449.
Abstract
ignored
We show that knockdown of Gpr27 reduces endogenous mouse insulin promoter
activity and glucose stimulated insulin secretion. Furthermore, we show that
Pdx1 is important for Gpr27's effect on the insulin promoter and insulin
secretion. Finally, the over-expression of Gpr27 in 293T cells increases
inositol phosphate levels, while knockdown... 阅读全帖 |
|
P*******e
发帖数: 27 | 26 After reading the comments about NIH, it seems to me people here has a
misunderstanding about the drug discovery process. People seem to believe
the drug discovery has always progressed like this: target---pathway---
cellular model---animal model---human beings. Indeed this is the well
publicized model or paradigm. But years ago, people used a phenotypic
screening to search for drugs without knowing targets, often directly on
animals. For anti-cancer drugs, many old ones were discovered without
... 阅读全帖 |
|
h****g
发帖数: 259 | 27 http://www.ncbi.nlm.nih.gov/pubmed/22446714
The pathobiological impact of cigarette smoke on pancreatic cancer
development (Review)
Authors: Uwe A. Wittel, Navneet Momi, Gabriel Seifert, Thorsten Wiech,
Ulrich T. Hopt, Surinder K. Batra
Please email to s******[email protected]
Thanks and Happy New Year! |
|
|
l**********1
发帖数: 5204 | 29 if cancer cell
'sox9'
pls refer
PMID 23201164
by Kopp JL et al.
Identification of Sox9-dependent acinar-to-ductal reprogramming as the
principal mechanism for initiation of pancreatic ductal adenocarcinoma.
(2012).
Cancer Cell. 22: 737-50.
PDF link:
HttP: //cmm.ucsd.edu/sander/sander/Home_files/Kopp_CancerCell_2012.pdf
>>
>>>
发信人: wdgca (全是骗银地), 信区: Biology
标 题: Re: genes in controlling cell morphology
发信站: BBS 未名空间站 (Wed Apr 10 16:44:27 2013, 美东)
做了啥treat，用的啥细胞都不说，那就木法猜。
> |
|
s******9
发帖数: 283 | 30 http://www.cell.com/abstract/S0092-8674%2813%2900449-2
Researchers at the Harvard Stem Cell Institute (HSCI) have discovered a
hormone that holds promise for a dramatically more effective treatment of
type 2 diabetes, a metabolic illness afflicting an estimated 26 million
Americans. The researchers believe that the hormone might also have a role
in treating type 1, or juvenile, diabetes.
The work was published today by the journal Cell as an early online release.
It is scheduled for the May 9 pr... 阅读全帖 |
|
s******9
发帖数: 283 | 31 http://www.cell.com/abstract/S0092-8674%2813%2900449-2
Researchers at the Harvard Stem Cell Institute (HSCI) have discovered a
hormone that holds promise for a dramatically more effective treatment of
type 2 diabetes, a metabolic illness afflicting an estimated 26 million
Americans. The researchers believe that the hormone might also have a role
in treating type 1, or juvenile, diabetes.
The work was published today by the journal Cell as an early online release.
It is scheduled for the May 9 pr... 阅读全帖 |
|
b*********a
发帖数: 105 | 32 International Journal of Hepatobiliary and Pancreatic Diseases (IJHPD)杂志近
期有一篇肿瘤的case report。有兴趣的发私信联系。 |
|
v********9
发帖数: 129 | 33 代朋友发帖，全文如下：
我叫陈熹，是张辰宇的学生。昨天有朋友告诉我，说你老板火了，在MITBBS被人“打脸
”了，让我去看一下。我心想，又是NBT这事，挺无语的，不过去看看国外的中国留学
生怎么看这个事情也好。看完我就郁闷了，我们课题组的文章怎么就是“垃圾”了，张
辰宇怎么就是“joker”了？其实以往对于这些事情我都是缄默的，搞科研的数据说话
，所以一直以来也在坚持做着这个领域，希望做出更多的东西来，弄明白一些问题，但
是心血被贬得一文不值，不吐不快。你说外洗地也好，说我是打手也好，我无所谓，我
就是想说说心里真实的想法。
1. 首先，我想澄清，我们的工作不是“垃圾”，张辰宇也不是“joker”。我们实验室
从2004年开始做miRNA，当时规模很小，就我一个人做，平台也刚开始建立，进展较慢
。但经过三年时间，miRNA的检测技术和功能研究我们基本都能做了，一个机缘巧合，
我们发现血清中普遍含有miRNA，从此我们从细胞内miRNA跳到了细胞外miRNA，并确立
了以研究细胞外miRNA为实验室的主要方向，期间的主要发现是：发现血清中有循环
miRNA；发现经由microvesicl... 阅读全帖 |
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S*********s
发帖数: 304 | 34 看十年内的此类paper，Nature/Cancer cell上有很多
就是发的那阵热闹一下，后面再也没人记得了。
我想同样是CNS,过十年二十年五十年，大家还会记得James Chen的cGAS as a
classical paper。
没有人会记得这篇还是那篇p53 pancreatic cancer nature paper. |
|
|
f******g
发帖数: 382 | 36 这个inhibitor抑制不好R172K。
IDH 很奇怪。别的target都是同家族的想找特异的inhibitor都找不到。IDH1和2很相似
，R132和R172 inhibitor却不通用。
Incyte 的pipeline里还有别的。 ruxolitinib在pancreatic cancer trial； 和lily
合作inflammation的trial； 和merck合作IDO 的药。对这些的预期拉高了股价。 |
|
r*****m
发帖数: 3619 | 37 和老子这一代留欧的王大成，留学期间直接贡献了一些方法学，Robert Huber 在诺贝
尔奖得奖致辞上特别提了他的贡献。当然他是一个学生。所谓晶体指导药物设计，那时
就吹起来了。
In 1970, I had begun work on the basic pancreatic trypsin inhibitor which
has later become the model compound for the development of protein NMR,
molecular dynamics, and experimental folding studies in other laboratories.
Work in the field of proteolytic enzymes and their natural inhibitors has
been continued and extended to many different inhibitor classes, proteases,
their proenzymes, and complexes betwe... 阅读全帖 |
|
s***l
发帖数: 10 | 38 刚刚在研究这个领域的文章，看到这个guang-hui liu在cell research上出现了几次
，频率很高，就搜了一下，结果吓了一跳，尽然有14篇之多。这个肯定是中国干细胞界
的新星吧，不然，在10多分的杂志上随便灌水，这个面子可是不小呀。
1.Gametogenesis in a dish [1422-1425]
Ying Gu1, Guang-Hui Liu1,2 and Juan Carlos Izpisua Belmonte1,3
2.The dawn of angiogenesis modeling: regenerating vasculature from human
pluripotent stem cells [3-5]
Fei Yi1,*, Jing Qu1,2,*, Guang-Hui Liu1,2 and Juan Carlos Izpisua Belmonte1,
3
3.Huntington's disease: Dancing in a dish [1627-1630]
Kejing Zhang1,*, Fei Yi1,*, Guang-Hui ... 阅读全帖 |
|
s**********e
发帖数: 2888 | 39 记得去年在Science上面看到报道，现在都已经快批准了，牛。
http://news.yahoo.com/us-regulators-fast-track-novel-leukemia-t
Washington (AFP) - US regulators on Monday put an experimental immunotherapy
agent on the fast track to market approval, after 89 percent of leukemia
patients in early trials saw their cancers disappear.
The personalized immunotherapy known as CTL019 was developed by the
University of Pennsylvania and was designated a "breakthrough therapy" by
the US Food and Drug Administration.
That means the experi... 阅读全帖 |
|
l******a
发帖数: 84 | 40 谢谢两位的回复。我个人对这个领域不懂，只是替亲戚寻人才。但好像美国这边已有公
司在做这方面的应用 ViaCyte， San Diego。下面是从他们网站上剪切来的，听起来很
有意思的一个approach:
ViaCyte's innovative product is based on the differentiation of stem cells
into pancreatic beta cell precursors (PEC-01™), with subcutaneous
implantation in a retrievable and immune-protective encapsulation medical
device (Encaptra® drug delivery system). Once implanted, the precursor
cells mature into endocrine cells that secrete insulin and other hormones in
a regulated manner to c... 阅读全帖 |
|
m**********3
发帖数: 706 | 41 Indeed, diabetic patients have increased risk of pancreatic cancer. |
|
b*******1
发帖数: 16 | 42 岗位一：吴虹课题组博士后工作人员 （2名）
主要职责：
1）独立开展科研工作，动手进行体内外实验；
2）协助PI负责实验室研究课题的设计和开展；
3）参与科研基金撰写；
4）协助培养研究生。
招聘条件：
1）品学兼优，身体健康，年龄在40岁以下；
2）近年来在国内外获得分子生物学、细胞生物学、肿瘤遗传学、药理学等相关专业博
士学位；
3）至少具备一项以下领域内的工作经验和文献发表：
a. 独立运用细胞和动物模型进行肿瘤药物的机理研究
b. 在分子生物学和细胞生物学领域有深厚的知识背景；
c. 在蛋白质分析、核酸分析和细胞生长分析等相关技术方面有丰富的经验；
d. 对治疗癌症的药物疗效和安全性评价方面有强烈的兴趣和研究背景 ；
4）有较强的团队合作精神，沟通协调能力强，动手能力强，工作细心，积极主动，能
持之以恒；
聘用待遇：
本职位将为你提供在生物学领域极具竞争力和创造性的工作机会。博士后工作人员薪酬
标准按照北京大学生命科学院和北大-清华生命科学中心相应规定及个人工作经验及能
力确定。
联系方式：
有意者请将本人应聘材料电子文档发至以下联络方式（请在应聘材料及邮件主题上注明
：姓名 ... 阅读全帖 |
|
z********8
发帖数: 818 | 43 ANGPTL8/Betatrophin Does Not Control Pancreatic Beta Cell Expansion 竟然发
在cell 了。 |
|
b**k
发帖数: 1585 | 44 Radiofrequency ablation for advanced ductal pancreatic carcinoma: is this ap
proach beneficial for our patients? A systematic review
[email protected]
/* */
非常感谢! |
|
D*a
发帖数: 6830 | 45 Red meat
After thoroughly reviewing the accumulated scientific literature, a Working
Group of 22 experts from 10 countries convened by the IARC Monographs
Programme classified the consumption of red meat as probably carcinogenic to
humans (Group 2A), based on limited evidence that the consumption of red
meat causes cancer in humans and strong mechanistic evidence supporting a
carcinogenic effect.
This association was observed mainly for colorectal cancer, but associations
were also seen for panc... 阅读全帖 |
|
n**e
发帖数: 2026 | 46 【 以下文字转载自 WaterWorld 讨论区 】
发信人: nile (nile), 信区: WaterWorld
标 题: 美国科学促进会《科学》大旗下的转基因谎言
发信站: BBS 未名空间站 (Fri Oct 30 10:18:45 2015, 美东)
今年初，美国科学促进会（AAAS）和皮尤研究中心（Pew Research Center）联合公布
的一项调查显示，88％的科学家认为转基因食品可安全食用，持这一看法的公众只有37
％。报告认为差距如此之大的一个可能原因是，三分之二的公众认为科学家对转基因食
品的健康后果没有清晰认识。这就是真正的原因吗？
根据报告全文，皮尤中心给19，984名科学家发出了调查问卷，3748人（18.8%）完成了
问卷调查。其中生物医学学科背景的科学家占50%。报告没有说明50%的生物医学背景是
否能够代表科学家总体的学科构成。更大问题是在这50%的科学家人群中，有多少人的
工作是与转基因作物的研究有关，以什么样的方式相关。报告没有提供任何信息。
由于这些关键信息缺失，88%的科学家相信转基因安全的说法毫无意义。在一个没有代
表性并且无法排除... 阅读全帖 |
|
|
b**k
发帖数: 1585 | 48 Immune Therapy in Pancreatic Cancer: Now and the Future?
Authors: Zhang, Yue; Choi, Minsig
Source: Reviews on Recent Clinical Trials, Volume 10, Number 4, December 201
5, pp. 317-325(9) |
|
n******d
发帖数: 680 | 49 对，能去当然会去，工资给的很不错。谈science伤钱嘛。
[在 desitin (desitin) 的大作中提到：]
:能去还是要去。
:有了一个亿，做不做pancreatic cancer就不重要了。
:资规模达到了了一个亿，年底搬到Cambridge，准备大干一票。我之前不做cancer，真
没 |
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b******n
发帖数: 4225 | 50 Curr Mol Med. 2016 Sep 26. [Epub ahead of print]
Long non-coding RNA: An emerging paradigm of pancreatic cancer.
Han T, Hu H, Zhuo M, Wang L, Cui JJ, Jiao F, Wang LW
email: [email protected]/* */
Thanks in advance |
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