S*****n
发帖数: 6055 | 1 呵呵。。。
我的题目的要求是把这些东西溶解在hexane里面，不能用其他溶剂或者混合溶剂。。。
至于形成啥样的micelle，甚至能否形成micelle，倒是不在乎。。。
PBD貌似会交联，注意保存。形成micelle貌似要好多天。还要不停的搅，对了，还有浓
度也是个问题，你看到溶胀是不是就是溶质太多了？
polybutadiene
到micelle了。 |
|
n******g
发帖数: 662 | 2 【 以下文字转载自 Chemistry 讨论区 】
发信人: nantsing (猛龙过江), 信区: Chemistry
标 题: Did anyone conjugate micelles with biomolecules?
发信站: BBS 未名空间站 (Sat May 3 01:44:19 2014, 美东)
Micelles were prepared by Phospholipid-PEG-COOH.EDC/NHS was used to activate
micelle surface to react with NH2-protein. However, never see the
conjugation is successful.
Very confused. Any suggestions? |
|
s****e
发帖数: 2934 | 3 这个PS-PBD的micelle难道没有文献吗？先查文献用什么样的分子量和怎么用溶剂。PBD貌似会交联，注意保存。形成micelle貌似要好多天。还要不停的搅，对了，还有浓度也是个问题，你看到溶胀是不是就是溶质太多了？
Solution Properties of Diblock Copolymers of Polystyrene-block-polybutadiene
这个文章有用吗
dibutyl phthalate是PS的选择性溶剂，把DBP和benzene混起来再把benzene挥发就得到micelle了。
PS |
|
n******g
发帖数: 662 | 4 Micelles were prepared by Phospholipid-PEG-COOH.EDC/NHS was used to activate
micelle surface to react with NH2-protein. However, never see the
conjugation is successful.
Very confused. Any suggestions? |
|
w******r
发帖数: 43 | 5 I just gived a talk about cryo-TEM's application for micelle.
I paste the key points. In my opnion, it is hard to do and the sample
preparation, you must change the concentraion. In dilute solution, you will
not
change the morphology of micelle in these work.
• Advantages:
1. TEM is a good local structural probe and the only technique that allows
real-space imaging of three-dimensional object at high resolution;
2. It is model-independent direct imaging of the local microstructure of
compl |
|
w********h
发帖数: 12367 | 6 (1)
SELF-DIFFUSION OF POLYMER-LIKE MICELLES
OTT A, MORIE N, URBACH W, BOUCHAUD JP, LANGEVIN D
JOURNAL DE PHYSIQUE IV
3: (C1) 91-103 MAY 1993
Document type: Article Language: English Cited References: 36
Times Cited: 5
Abstract:
We present a short review of fluorescence recovery (FRAP) experiments done
with polymer-like micelles, allowing the study of self-diffusion in these
media. The observed behavior is varied, specially in the semi-dilute regime
where one can observe reptation |
|
w******r
发帖数: 43 | 7 what is your means about phase diagram?
Block copolyme can form different morphologies in dilute solutions.
With increase of concentration, the micelle solution could be BCC, FCC
structure. TPL has some papers about the characterization and kinetic
transition between BCC<>FCC.
Bates reported various micelle morphlogoies formed
from PBD-PEO diblock copolymers. They did a lot of cryoTEM and got very nice
images.
(1) Jain, S.; Bates, F. S. Science 2003, 300, 460.
(2) Won, Y.-Y.; Brannan, A. K.; Dav |
|
s****e
发帖数: 2934 | 8 已知amphiphilic block copolymer B-b-C能形成micelle
nonpolar的organice solvent，加入20％的nonpolar homopolymer A， 20％×15％＝3
％的B-b-C，能形成micelle吗？ |
|
c*w
发帖数: 50 | 9 We know that when the Csurf. > cmc, the micelles form in the aqueous
surfactant solution.
My question is: take Tween 80 as an example. If the solution contains 90%
tween 80 and 10% water(v/v), is it still a micelle solution?
Thanks for the answer! |
|
s****e
发帖数: 2934 | 10 micelle我还知道点，和micelle无关我就不来参合了。我估计也会去想要不要用二绿甲
烷完全是瞎猜。 |
|
n******g
发帖数: 662 | 11 I did follow Pierce instructions for COOH to amine-protein link in water
solution using EDC/NHS activation or just EDC activation.
Its protocol did work well for polymer microbead-COOH and amine-protein
linking. But it seems not working if I change microbead-COOH to micelle-COOH.
I do see people using maleimide-PEG-Phosphilipid to react with thiolated
protein.
without |
|
A******y
发帖数: 2041 | 12 Are you sure the one you buy is not already activated? EDC activation
require anhydrous condition (just go search online). Once Osu-ester is form
, it can react with amine in aqueous condition. If you think it is the
micelle, then you have to re-think you sequence of reaction.
COOH. |
|
c********n
发帖数: 7 | 13 Micelle is not another kind of macromolecule, not any kind of
new maerials, it is just a morphology, a form of aggregation
with core-shell shape. |
|
w********h
发帖数: 12367 | 14 sure...
there are some papers descibing how to treat micelles as reptating long
chains...I just read some of them last night..wait a few minutes, I will get
them for u... |
|
z**h
发帖数: 224 | 15 我们知道，block copolymer 在融体的构形以被Bates 等人研究
多时，micelle 在溶液中的相图是否有人作过？是否也取决于
kai*N vs phi ? |
|
z**h
发帖数: 224 | 16 what determines the shape of the micelle?
Talmon, |
|
c*****e
发帖数: 238 | 17 Generally the answer is no.
But this is always getting complicated with chemists, they say that if the
concentration is high enough, something so-called microemulsion is formed and
that is some sort of transition.
In my view, micellation does not involve cooperative effect or long range
correlation, thus is not a phase transition. |
|
c*s
发帖数: 2145 | 18 MICELLE上弄个PORE，引入酸性集团，热相应集团，这些花样似乎都很普通了阿 |
|
s****e
发帖数: 2934 | 19 ☆─────────────────────────────────────☆
cds (红毛呼呼猪) 于 (Mon Sep 25 22:12:54 2006) 提到:
MICELLE上弄个PORE，引入酸性集团，热相应集团，这些花样似乎都很普通了阿
☆─────────────────────────────────────☆
nomos (秩序) 于 (Wed Sep 27 07:40:49 2006) 提到:
俺也不知道啊，反正混了一S/J/A
fission
☆─────────────────────────────────────☆
yueke (小鱼儿) 于 (Tue Sep 26 20:03:05 2006) 提到:
这不是我国内老板做的么？实在不懂，啥意思啊，一个fusion发在angew，一个fission
发jacs
fusion, fission |
|
h***s
发帖数: 111 | 20 It depends on the CMC of B-b-C. If CMC < 3%, I think it will. But it might
not be able to encapsulate A in the micelles if A is fully soluble in your
solvent.
＝3 |
|
s****e
发帖数: 2934 | 21 那就是说micelle不受viscosity的影响吗？比方CMC<3％，加了20％的homopolymer这个
溶液的粘度是相对比较大了。 |
|
r*******e
发帖数: 83 | 22 要看你加入得homopolymer跟B或C嵌段有没有相互作用得，如果没有作用，聚合物胶束还
是相对比较稳定得
那就是说micelle不受viscosity的影响吗？比方CMC<3％，加了20％的homopolymer这个
溶液的粘度是相对比较大了。 |
|
m******k
发帖数: 447 | 23 How to avoid the formation of micelles when using PVA as the surfactant in
the suspension polymerization?
It's so hard to filter when the suspension reaction is done. So many papers
showed they used filtration method to separate particles from water phase. I
don't know how they make it. I cannot use centrifuge and decant method
because of ....
This question bother me so much. Tons of thanks for any suggestion. |
|
m******k
发帖数: 447 | 24 Most particles are around 10 microns. While I cannot filter to collect
particles. I guess there are some emulsion particles inside. Do you know any
way to avoid the formation of micelles or anyway to filter the suspension?
Tons of thanks. |
|
t***u
发帖数: 20182 | 25 不知道啊，一般搞micelle应该这么做吧，用良溶剂把polymer溶掉，然后慢慢滴加到某一
block的不良溶剂。先用THF，然后滴加hexanes？ |
|
w******r
发帖数: 43 | 26
For the block copolymer micelles:
DLS can tell you the Rh of your micelles, it is best for sphere.
The best way is cryo-TEM. You can watch the morphology directly.
The convient and good is SAXS, you fitting the fact S(q) with different model.
The most accurate way is SANS. The recent results show that the micelle core
is not exactly close-packed.(Mortensen)
You can also measure the d1 by NMR to infer the micelle structure.
Another very good way is fluorescene spectrum. |
|
c*s
发帖数: 2145 | 27 hehe，已经过了12点， 算是明天把
MULLER 这次将的很GENERAL的题目， 也就是把他们组里的工作介绍了以下。
他们这两年工作比较有特色的就是三嵌段共聚物的合成， 形貌，和自组装
这个三嵌段的特色在形貌，他们称之为“JANUS”
janus是古罗马的一个神， 前后都有脸，分别是面向过去和未来
JANUS是个很形象的比喻， 因为他们的聚合物形成MICELLE以后， 象两个半球组成
的。三嵌段是由St，BUTADINE，MMA三种单体通过阴离子聚合得到，由此出发，可以得
到多种形貌：
1。 这个聚合物SPIN COATING成膜，PMMA，PSt形成lamella，PB形成CYLINDER夹在
中间 TEM显示了这种结构，CYLINDER大概几个那迷
2。 这个聚合物SPIN COATING成膜，假如铰链剂， 使PB铰链，然后假如溶剂，在
溶液中形成MICELLE， 这个MICELLE如果沉积在MICA上， 形成一个“章鱼”形状的颗粒，
PS是一个原形的头，PB在下面形成几个小的DORMAIN，象鱼眼睛，最下面，趴在MICA上的
是PMMA。想章鱼的爪子。
MICELLE可以分 |
|
m*****e
发帖数: 1506 | 28 你的micelle是水溶液么？你的micelle进了gpc以后在大量dmf里面应该会dissociate
如果你的micelle有charge，比如PAA，那么DMF里需要加些LiBr，否则PAA很有可能把在
柱子上下不来。不知道你为什么要用GPC表征micelle。。。 |
|
a*****5
发帖数: 94 | 29 Title: Investigating the solubilization of oil in Gemini surfactant micelles
by using lattice Monte Carlo simulation
Abstract: In this study we investigated the mixing behavior of the Gemini
surfactants (T11H1SnH1T11 ,n=2,4,6,8) and oils (Tn ,n=2,3,4 ) in aqueous
solutions by using lattice Monte Carlo simulation method. The results show
that adding oil to the surfactant solution results in decreasing the CMC.
Investigating the changes of the CMC vs. the spacer length shows that
increasing the sp... 阅读全帖 |
|
b***u
发帖数: 14 | 30 我做micelle的时候，也这么测过，同样的问题。
个人觉得可能的原因，
聚合物链构象转变需要时间。micelle的时候，链(特别是hydrophobic的那段)是
folding的，需要时间回到random coil的构象。这个时间还应该包括就是通过溶剂的交
换从H2O到DMF的时间。
而且以前做的时候还发现，注入micelle的水溶液，在溶剂出峰的时间总是看到很奇怪
的东西。可能包括了水和柱子里面残留的乱七八糟的东西。 |
|
f******k
发帖数: 5329 | 31 他的意思应该是把drug load到micelle上。
应该要找一种大小和你的micelle差不多的酶吧。你的micelle多少nm? |
|
p*******n
发帖数: 63 | 32 这篇文献没有电子版，但对我来说很重要，求求好心人帮帮忙！
Author(s): VANDIJK HJM
Source: NETHERLANDS MILK AND DAIRY JOURNAL Volume: 46 Issue: 2 Pages: 101-
113 Published: 1992
Title：THE PROPERTIES OF CASEIN MICELLES .6. BEHAVIOR ABOVE PH-9, AND
IMPLICATIONS FOR THE MICELLE MODEL
thanks a lot!!
z**********[email protected] |
|
发帖数: 1 | 33 我只是做过很多tail anchored protein，有一些经验。
我觉得你说的已经可以充分证明作用区域在TMD。如果现在设想的单纯用TMD的办法又表
达量很低，那么可以考虑swap TMD。就是找另一个在细胞膜上毫不相干的跨膜蛋白质，
挑它的TMD，就找中间20个非常疏水的AA应该没有问题，swap到你蛋白质里，然后做
CoIP。这个不相干的蛋白质作为negative control。这样可以保证你的蛋白质TMD外围
信号不变。
但是要提醒你，如果蛋白A和蛋白B都是跨膜蛋白，CoIP出来的结果可能是假阳性，特别
是你用的detergent只有NP40，并且negative control是cytoplasmic protein，比如
free GFP。那么AB会CoIP，negative control不会。因为NP40是弱detergent，我怀疑
membrane在buffer里被打成了micelle， 所以AB同在micelle里一起被IP。如果用RIPA
buffer你会发现AB不CoIP了，当然你也可以认为RIPA buffer太强了。所以除了CoIP最
好有其他证据证... 阅读全帖 |
|
n*****7
发帖数: 19 | 34 对polymer 自组装而言，dn/dc是分析zimm plot 的前提，可以得到聚集的Mw,除以单个
链分子量得到aggregation No.，即每个micelle有多少个链，然后结合浓度就知道单位
体积多少了micelle... |
|
q****i
发帖数: 6923 | 35 Utilization of Alkoxysilyl Groups for the Creation of Structurally
Controlled Siloxane-Based Nanomaterials
Kazuyuki Kuroda, Atsushi Shimojima, Kazufumi Kawahara, Ryutaro Wakabayashi,
Yasuhiro Tamura, Yusuke Asakura, and Masaki Kitahara
Chem. Mater., 2014, 26 (1), pp 211–220
Preparation and Formation Mechanism of Silica Microcapsules (Hollow Sphere)
by Water/Oil/Water Interfacial Reaction
Masahiro Fujiwara, Kumi Shiokawa, Yuko Tanaka, and Yoshiko Nakahara
Chem. Mater., 2004, 16 (25), pp 5420–5426... 阅读全帖 |
|
w******r
发帖数: 43 | 36 These papers are non-equilibrium system, because they use PS as one of blocks.
PS has high Tg than rm T.
There are a lot work is about both the hydrophobic and hydrophilic have low
Tg,
so they can reach equilibrium.
Bates have a paper in JPS 2002 using cryo-TEM to characterize the micelle
morphology.
I know amphiphilic block copolymer can form spherical, cylinder, versicle,
flower and onion and networw or bicontinuous micelles. |
|
s****e
发帖数: 2934 | 37 多谢斑竹！希望不会被我烦死。
可是我不明白斑竹说的colloidal crystal是什么？是指形成的colloid之间有相互作用么
？you said "I doubt that this is possible", and I would like to know what is "
some special fluid dynamics treatment" too. could you discuss it in detail?
Do you think I can use ionic end group( cationic on polymer A and anionic on
polymer B) to make them organize firstand then react?
Actually, my system is particle A in micelle A' and particle B in micelle B',
do you still think the hard sphere will attract each other strongly a |
|
s****e
发帖数: 2934 | 38 理解错我的意思了，我说的particle不是colloid particle我说的particles是hard
particles比如alumina 或者金属什么的，外面包着block polymer形成micelle。我还想
顺便问一下colloid和micelle有什么区别呀？我以为就高分子领域他们是同一个东西呢？ |
|
c*****e
发帖数: 238 | 39 有3篇文章是关于polymer的，而且是traditional polymer materials science
Cylindrical Block Copolymer Micelles and Co-Micelles of Controlled Lengt
h and Architecture Xiaosong Wang, Gerald Guerin, Hai Wang, Yishan Wang,
Ian Manners, and Mitchell A. Winnik http://www.sciencemag.org/cgi/conten
t/abstract/317/5838/644?etoc p. 644
Block Copolymer Assembly via Kinetic Control Honggang Cui, Zhiyun Chen,
Sheng Zhong, Karen L. Wooley, and Darrin J. Pochan http://www.sciencemag
.org/cgi/content/abstract/317/5838/647?eto |
|
l**********g
发帖数: 55 | 40 Somebody told me that if the Tg of the polymer is lower than R.T., the
micelle it formed should be oberserved using cyro， is it true or not?
anybody could tell me in what situation should cyro be used for micelle
observation? |
|
j*********6
发帖数: 371 | 41 我直接把高分子micelle溶液注入GPC后，发现得到的峰变宽了。但是我先把水吹干，用
溶剂把高分子溶解后再做GPC，所得峰就和形成micelle之前的高分子的峰形一模一样，
这是怎么一回事呢？ |
|
h****z
发帖数: 1996 | 42 是micelle有个团聚数的原因么 形成micelle后一个团里面有多于一个chain
相当于分子量略微变大了 |
|
m*****e
发帖数: 1506 | 43 你的GPC是什么流动相？micelle在柱分离过程中可能有dissociation。你把水吹干了就
没有相分离了，就没有micelle了，当然得到的就是single polymer chain。
GPC拖尾很可能是你的polymer把在柱子上下不大来导致的。 |
|
z*******o
发帖数: 136 | 44
可能让你们误解了，我发现当酶的浓度为几个units/mL 时micelle会在两三个小时内被
水解完，而且前几分钟非常快，随后水解速度递减。
所以， 如果血液中酶的浓度是几十个Units的话，我的micelle根本就没法用于药物载
体材料，几分钟都被水解了。 |
|
A*****8
发帖数: 8590 | 45 Report Information:
Publication date: 11/07/2012
Number of Pages: 145
Report Details:
In 2011, the medical device market worldwide was worth $309bn, with high
single-digit growth from the year before . Despite being a vast and mature
market, there are number of sectors likely to experience fast growth during
the next 10 years. Much of this growth is linked to the prevalence of a
number of diseases worldwide, as well as an aging population. The rise in
neurological disorders will drive both the d... 阅读全帖 |
|
S******8
发帖数: 24594 | 46 中新网5月16日电 综合美国《世界日报》报道，涉嫌在纽约曼哈顿东村街头殴打华裔耆
老阮文辉致死的20岁非裔嫌犯丕犹(Jamie Pugh)，14日在曼哈顿刑事法院过堂。承办该
案的检察官向法官提出以二级谋杀、企图抢劫以及一级攻击三项罪名起诉，且不得交保
。法官最后同意检察官提出的这三项预控罪名，且同意不交保。出庭过堂的丕犹当庭还
押，法院将于16日对丕犹提出正式控诉罪名。阮文辉的遗孀和三个女儿在律师罗佰能的
陪伴下出庭聆听，看到丕犹时，他们都有些激动。阮文辉小女儿步出庭外时表示，看到
丕犹的时候，“感到一阵恶心”。
68岁的华裔耆老阮文辉9日晚间在离住家不远的曼哈顿东6街145号前，被丕犹痛殴
致死，丕犹在12日凌晨被逮捕，被捕时，他身穿印有刺青图腾的黑色外套，染着一头金
发，一脸呆滞。14日他出庭过堂时，仍身穿被捕时同件外套，毫无表情，眼神涣散。丕
犹的母亲Harlotte Pugh-Douglas也出席，坚信儿子是“无辜的”的她向“DNAinfo”记
者表示，儿子在事发前遭友人诱惑吸食毒品“莫莉”(Molly)，事发时“他的脑袋不在
正常状态”。
助理检察官向法官表示，从监视录像器... 阅读全帖 |
|
F*****y
发帖数: 3877 | 47 你11－12年来的ufl，研究金属离子键光谱相关的，你觉着我把你的反美言论发到下面
这些人，他们能不能记起来你是哪位？
Jason E. Butler
Professor 352-392-2591
[email protected]/* */ Dynamics of Complex Fluids, Suspension and Multiphase
Fluid Mechanics, Polymer Dynamics and Rheology
Anuj Chauhan
Professor 352-392-2592
[email protected]/* */ Microfluidics, Drug Delivery, Biomaterials, Complex
Fluids
Oscar D. Crisalle
Professor 352-392-5120
[email protected]/* */ Process Control Engineering, Manufacture and
Optimization o... 阅读全帖 |
|
发帖数: 1 | 48 化学/高分子/材料 求推荐审稿机会
才完成PhD,准备开始申请绿卡,在这里向各位大牛求审稿机会
涉及的领域主要有polymer/macromoleculear chemistry, self-assembly, drug
delivery, organic electronic materials, hybrid materials, block copolymer,
stimuli-responsive polymer, micelles and hydrogels等
email:[email protected]
如需要更多信息（比如CV或者publication list）请email我，多谢多谢 |
|
发帖数: 1 | 49 化学/高分子/材料 求推荐审稿机会
才完成PhD,准备开始申请绿卡,在这里向各位大牛求审稿机会
涉及的领域主要有polymer/macromoleculear chemistry, self-assembly, drug
delivery, organic electronic materials, hybrid materials, block copolymer,
stimuli-responsive polymer, micelles and hydrogels等
email:[email protected]
如需要更多信息（比如CV或者publication list）请email我，多谢多谢 |
|
l***f
发帖数: 618 | 50 今天收到一个审稿，RSC的polymer chemistry.
我最近太忙同时也不是我的研究方向，贡献给合适的人。请相关方向的人给我发信，把
你的简介和publication给我，我推荐你。并希望你能在那里用你的official 的email
先注册好帐号。
"Camptothecin prodrug block copolymer micelles with high drug loading and
target specificity" |
|
