a*****i 发帖数: 679 | 1 最近做一个luminex上的assay,空白对照(不加模板DNA)的信号会随机升高,多个空
白对照之间的背景值变高现象无规律可循。已经换过各种试剂,引物,PCR条件,始终
不能解决。有没有做过luminex的朋友分享一下经验?多谢。 |
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w******e 发帖数: 1187 | 2 请教luminex的multiplex assay system跟microarry相比有什么好处?
感觉需要用到FACS的话麻烦多多啊~而且两者都有很多共同的局限
多谢 |
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w******e 发帖数: 1187 | 3 ding. luminex也算是表现不错的biotech公司了,大家对工业界的动向
都没兴趣吗? |
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R****n 发帖数: 708 | 4 luminex的技术是以beads为基础的,array是玻璃板。现在这两个平台跟next
generation sequencing 比都是昨日黄花了。
要是做application估计genetics上用这个还不如real-time PCR更通行。工作就去吧,
比postdog强。 |
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w******e 发帖数: 1187 | 5 有个idea,需要问luminex的人几个简单的技术问题,不涉及机密不是陶瓷,帮我
代问都行。哪位筒子有路子麻烦给介绍一下吧。非常感谢! |
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w******e 发帖数: 1187 | 6 想搞点他们的beads来试试multiplex,居然被告知他们的beads只在luminex system
里work,不适用于FACS。。。靠难道他们的system不是基于flow cytometry的嘛。。。
同时借问,还有没有什么公司生产>4种不同颜色,其他规格一致的magnetic
microbeads
吗?多谢! |
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y****6 发帖数: 196 | 7 在用Luminex 检测时,我想加入(spike) 一个外源蛋白作为内参。要求有两点:一是和
哺乳动物蛋白同源性越低越好,这样交叉反应的可能性低。二是可以选择的抗体比较多
。GFP是个不错的选择,但是不知道它会不会干扰后续的荧光检测?luciferase 怎么样
?还有什么更好的建议吗?
★ 发自iPhone App: ChineseWeb 1.0.1 |
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H**********J 发帖数: 351 | 8 • Ph.D. or Master in pharmaceutical sciences, immunology, biology
or a related field
• Minimum of 4 (Ph.D.) or 7 years (Master) of experience in assay
development for large molecules
• Experience with GLP is a must
• Familiar with pharmaceutical R&D process
• Demonstrate capability and confidence to build, train and lead a
group
• Hands-on experience with ELISA, EIA, and qRT-PCR methodologies;
strong interest and demonstrated skills with stat... 阅读全帖 |
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w****r 发帖数: 245 | 9 大侠,分类都分好了,求神签
# Basic Materials, Agricultural Chemicals
AGU Agrium Inc.
AVD American Vanguard Corp.
CAGC China Agritech Inc.
CF CF Industries Holdings, Inc.
CGA China Green Agriculture, Inc.
CMP Compass Minerals International
COIN Converted Organics Inc.
IPI Intrepid Potash, Inc.
MON Monsanto Co.
MOS Mosaic Co.
NOEC New Oriental Energy & Chemical
POT Potas... 阅读全帖 |
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E*********r 发帖数: 4984 | 10 今早有人问我对于PACB的看法,不好意思,今天蛮多事情,还来不及马上回复。 说实
在对于下一代测序本人不是专家,不过也随便讲几句。其实Mutate大牛是搞测序的,具
体可以问他。 我曾经到过Ion Torrent看过他们一个Demo,不过感觉比Illumina的方法
差一些。PACB的话,我以前有一个同事在那边工作,后来离开了,问他原因,他说
Bored。 至于长片段有没有用,我的观点是肯定好一些,但不是绝对的。下一代测序是
把短片段通过软件接起来,目前大概Ion Torrent的小片段就100多个碱基,然后串成整
个Genome Sequence。长片段连接的准确率高,短的就差一些,不过还要看软件怎么写。
值得一提的是,测序在临床上的使用还只是开始,能够解决的问题不多,主要是遗传性
疾病,最大的用途是找SNP,现在的Pharmacogenomics很热,也所谓的个体化医疗方案
。现今了解到不少疾病或者药物反应和某些SNP有很大的关系,因此筛选SNP成为分子诊
断非常重要的一个环节。不过测序有点用牛刀,有些东西,一个RT-PCR就可以解决,
Luminex也有专门筛选SNP的检测法,我... 阅读全帖 |
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E*********r 发帖数: 4984 | 11 问题是根本不需要用到下一代测序,用现有的桑格测序法就可以解决。拉个PCR,去测
一下序列。在诊断领域下一代测序只是做胎儿的Inborn Errors有一些,其它方面我还
真没听说过多少。我和Ion Torrent的Product Manager还有Illumina的都很熟,谈起临
床的问题,他们也承认目前应用不多。
Luminex现在有专门做SNP的试剂盒。不需要测序也可以很快找到SNP。 |
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E*********r 发帖数: 4984 | 12 为啥要涨那么多? 测序在临床上开展的障碍很大,我们医院早就要求医生尽量不要使
用分子诊断的方法。 能够一个RT-PCR或者Luminex的方法解决的问题干啥要测序? |
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w**k 发帖数: 6722 | 13 很显然,一个RT-PCR或者Luminex的方法解决不了问题
羊穿才能解决问题,没有哪个准妈妈愿意来这么一针 |
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H*Q 发帖数: 725 | 14 【 以下文字转载自 Biology 讨论区 】
发信人: HiQ (L.L.Ben), 信区: Biology
标 题: Open position of Associate Scientist @ Boston
发信站: BBS 未名空间站 (Thu Mar 23 12:51:26 2017, 美东)
https://career012.successfactors.eu/career?company=merckgroup&career_job_req
_id=159751&career_ns=job_listing&navBarLevel=JOB_SEARCH
A career with us is an ongoing journey of discovery: our 50,000 people are
shaping how the world lives, works and plays through next generation
advancements in healthcare, life science and performance materials. F... 阅读全帖 |
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z**c 发帖数: 7595 | 15 看看当年:
(以下是新语丝公开资料。 如有冒犯,敬请原谅)
巴义芳,建筑师,加拿大,多伦多
曹涵,博士, 美国Bionanomatrix公司CTO
曹化哲,金融硕士及MBA,普天寿公司杰出地产经纪
曹焕,工程师,泉州市鲤城黄石机械有限公司外贸经理
Cao Linqiu,博士,Research Scientist,荷兰DMV International
曹乾,博士,副教授,东南大学医疗保险系
曹兴龙,法律硕士,民商法学博士生,律师,浙江西子律师事务所
曹耀月,国电南京自动化股份有限公司
曹子谊,教授,山东师范大学生命科学学院
蔡卫,计算机工程与工程技术硕士,美国北德克萨斯大学工程学院
Chan Jane, 博士, Sr. Analyst, US West Financial
Chen Biao, 生物医学工程博士, Principal Scientist, Hologic, Inc.
陈昌和,硕士,福建省交通厅
陈大兵,博士研究生,美国明尼苏达大学
Chen Dong, 博士,Senior Staff Scientist, Veeco Instruments
Chen Guangjion... 阅读全帖 |
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d***e 发帖数: 243 | 16 知道有Affymetrix,Illumina,Luminex, ABI和HRM之类路线,那位指点一下哪个做多
位点(比如CYP450 基因位点)的话性价比好点? |
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d***e 发帖数: 243 | 17 生物医学检测上应用很多,定量PCR仪,测序仪,液体芯片都能用到。检测仪器厂家如
Life tech, BioRad, Luminex都可调研一下。
统光学在 |
|
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t*e 发帖数: 47 | 19 BD CBA
Luminex xMAP technology
eBioscience CytoMix
Meso Scale Discovery technology |
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p*******r 发帖数: 4048 | 20
the only difference is it costs a lot more... |
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w******e 发帖数: 1187 | 21 han... isn't this company some kind of rising star thou?? |
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w******e 发帖数: 1187 | 22 已经是同一平台的第四篇paper了。不过话说这哥们的paper 从来都不高,
连FDA approved的药都是发在jbc上。会不会偏应用的都发不高?话说
我查luminex的paper也都发在没听过的journal上。。。 |
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w******e 发帖数: 1187 | 23 it's very easy to couple aptamers to sensor. in fact sensor field
is dominated by aptamer as affinity agent as the stability &
reusability is a big deal for sensors.
back to your questions, you can do thiol-gold, EDC-NHS, biotin-SA...
all you need is to synthesize the aptamer w/ desired functional group.
so are you doing arrays? or beads, like luminex? you may want to check
out larry gold's recent publication
I'd certainly like to, if it doesn't take too much time. any suggestions?
I'm confiden... 阅读全帖 |
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w******e 发帖数: 1187 | 26 thx. 那个啥公司,如果是proteomics的话,估计是luminex?号称有100种组合呵呵 |
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a*****g 发帖数: 543 | 27 luminex
Rules base medicine (now charles river owned) has Rodent and Human cytokine
multiplexing service.
profile |
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H**********J 发帖数: 351 | 28 没人感兴趣吗?o(╯□╰)o
• Ph.D. or Master in pharmaceutical sciences, immunology, biology
or a related field
• Minimum of 4 (Ph.D.) or 7 years (Master) of experience in assay
development for large molecules
• Experience with GLP is a must
• Familiar with pharmaceutical R&D process
• Demonstrate capability and confidence to build, train and lead a
group
• Hands-on experience with ELISA, EIA, and qRT-PCR methodologies;
strong interest and demonstrated s... 阅读全帖 |
|
m***r 发帖数: 21 | 29 Senior Research Associate - Target Discovery & Validation Job, Novo Nordisk
Inc.
Location: Seattle, WA, US
Novo Nordisk is a world leader in protein therapeutics. We are determined to
discover and develop superior biologics to control chronic inflammatory
conditions and continue significant innovation in this area.
We have established a research site in Seattle, aiming at employing 80
people by the end of 2011. We are looking for:
Auto req ID 4754BR
Title: Senior Research Associate - Target Disc... 阅读全帖 |
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D*a 发帖数: 6830 | 30 有谁用过测小鼠外周血IGF-I的Elisa或者EIA的kit?
现在用一个luminex的用beads的,打算换。
请问谁用过的或者确实知道哪家的管用的?
另外手里有一批血清/组织想试试western,谁知道测IGF-I 管用的抗体。。。
求私信,求关注 =.= |
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s********n 发帖数: 248 | 31 我想做cytokine bead array比较wildtype 和mutant 的mouse GM-CSF dendritic cell
的cytokine expression区别。之前的数据表明,mutant GM-CSF DC有增强的migration
,和antigen presentation。我还想比较一下cytokine secretion。
目前有BD的mouse inflammation kit,还有life technologies的luminex kit (10 and
20).大家谁用过他们的或者别家kit,能帮忙推荐一下吗? |
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a*****i 发帖数: 679 | 33 multiplex PCR 检测20个基因的point mutation,用的magnetic beads。模板是human
genomic DNA。有什么建议吗? |
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s*****n 发帖数: 64 | 34 We made an extract from human tissue products with DMEM/10%FBS and added it
to human fibroblast. Initially, the cells attached to the plate though they
looked crappy. overnight, they all rounded up and died.
Luminex mulplex analysis showed the extract contained ~300 pg/mL IL-1a,6, 8,
600 pg/mL MCP-1, 800 pg/mL TGF-beta 1, 2 and 2 ng/mL PDGF-AA,BB.
Has anyone heard of cytokine storm in cell culture? |
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a********k 发帖数: 2273 | 35 你们不用luminex?还有mass array也可以。
说实话taqman最容易,我们实验室一天做几百个病人,每个也是你这么多SNP |
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m*****r 发帖数: 1164 | 38 luminex这公司模式搞不太懂,好像很多公司都有他家产品的销售和服务。 |
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H*Q 发帖数: 725 | 39 https://career012.successfactors.eu/career?company=merckgroup&career_job_req
_id=159751&career_ns=job_listing&navBarLevel=JOB_SEARCH
A career with us is an ongoing journey of discovery: our 50,000 people are
shaping how the world lives, works and plays through next generation
advancements in healthcare, life science and performance materials. For 350
years and across the world we have passionately pursued our curiosity to
find novel and vibrant ways of enhancing the lives of others.
Your role:
W... 阅读全帖 |
|
d******e 发帖数: 14 | 40 【作者】:
1. Rizzi G, Zhang YJ, Latek R, Weiner R, Rhyne PW.
2. Rhyne PW, Wong OT, Zhang YJ, Weiner RS.
【文题】:
1. Characterization and development of a Luminex-based assay for the
detection of human IL-23
2. Electrochemiluminescence in bioanalysis
【期刊名,年份,卷,起止页码】:
1. Bioanalysis. 2010 Sep;2(9):1561-72.
2. Bioanalysis. 2009 Aug;1(5):919-35.
【链接】:
【求助者email】:
d********[email protected]
【求助者是否发包子】: |
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k****o 发帖数: 589 | 41
做过三个月Luminex和MALDI,当时是帮着一个新PI建立cancer biomarker的分析平台。
另外很想
学高通量筛药,书一直在看可是没有上手机会。
有个问题一直不太清楚,公司招intern是为了雇个廉价劳动力去干些辅助性工作呢,还
是为了让
intern学习新东西?如果只是招技术工的话,我的经验肯定比不上专攻药学的。 |
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H**********J 发帖数: 351 | 42 大家帮帮忙哈~
• Ph.D. or Master in pharmaceutical sciences, immunology, biology
or a related field
• Minimum of 4 (Ph.D.) or 7 years (Master) of experience in assay
development for large molecules
• Experience with GLP is a must
• Familiar with pharmaceutical R&D process
• Demonstrate capability and confidence to build, train and lead a
group
• Hands-on experience with ELISA, EIA, and qRT-PCR methodologies;
strong interest and demonstrated skills w... 阅读全帖 |
|
m***r 发帖数: 21 | 43 Senior Research Associate - Target Discovery & Validation Job, Novo Nordisk
Inc.
Location: Seattle, WA, US
Novo Nordisk is a world leader in protein therapeutics. We are determined to
discover and develop superior biologics to control chronic inflammatory
conditions and continue significant innovation in this area.
We have established a research site in Seattle, aiming at employing 80
people by the end of 2011. We are looking for:
Auto req ID 4754BR
Title: Senior Research Associate - Target Disc... 阅读全帖 |
|
w***y 发帖数: 2537 | 44 【 以下文字转载自 Stock 讨论区 】
发信人: ll111 (Stealth), 信区: Stock
标 题: Events and ER for week August 2-6
发信站: BBS 未名空间站 (Sun Aug 1 22:47:17 2010, 美东)
Monday, Aug. 2
10 a.m.: ISM Manufacturing index on business activity in July, released by
the Institute for Supply Management.
10:15 a.m. Federal Reserve Chairman Ben Bernanke speaking on "challenges for
the economy and state governments."
4 p.m. Treasury Secretary Timothy Geithner speaking on the next steps for
financial reform.
Tuesday, Aug. 3
8:30 a.m.: P... 阅读全帖 |
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v**********m 发帖数: 5516 | 45 You guys now are able to listen to these web-castings now. Enjoy it!
http://jpmorgan.metameetings.com/webcasts/healthcare11/ondemand
individual Company Presentations
A
Abbott
Abiomed Inc. (available to Feb. 5)
Ablynx N.V.
Accretive Health Inc.
Accuray Inc.
Acorda Therapeutics, Inc. (available to Feb. 11)
Actelion Ltd
Aetna Inc. (available to Feb. 8)
Affymetrix Inc.
Agilent Technologies Inc.
Albany Molecular Research Inc.
Alere Inc (available to Feb. 11)
Alexion Pharmaceuticals, Inc. (not availab... 阅读全帖 |
|
v**********m 发帖数: 5516 | 46 You guys now are able to listen to these web-castings now. Enjoy it!
http://jpmorgan.metameetings.com/webcasts/healthcare11/ondemand
individual Company Presentations
A
Abbott
Abiomed Inc. (available to Feb. 5)
Ablynx N.V.
Accretive Health Inc.
Accuray Inc.
Acorda Therapeutics, Inc. (available to Feb. 11)
Actelion Ltd
Aetna Inc. (available to Feb. 8)
Affymetrix Inc.
Agilent Technologies Inc.
Albany Molecular Research Inc.
Alere Inc (available to Feb. 11)
Alexion Pharmaceuticals, Inc. (not availab... 阅读全帖 |
|