p*******r
发帖数: 4048 | 1
I am really starting to doubt that saying. It might be a noble lie.
Now I think, the virtue and joy in giving is really because you mentally
construct an union between you and him/her. Would it give joy if he/she
doesn't like you gift?
I increasingly think that this virtue is a derived virtue from the fact that
we all like to receive. Therefore, the virtue of romantic love lies not in
giving but in forming unions.
In a sense, romantic love is an isolating experience. Daiyu said
孤标傲世携谁隐，一样花开为底迟。 |
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j********r
发帖数: 156 | 3 The experiment is to isolate blastocyst for cloning mouse embryonic stem
cells. Can anyone give me a
protocol? I couldn't find any on the web. Also I was told to start with 21
days mice, and to mate them on day
23 after stimulating the females with hormones (PMS and hCG). Are they too
young?
Thanks a lot! |
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z***x
发帖数: 80 | 4 I have no experience of isolating RNA from cartilage but need to do it soon.
Cartilage contains a lot of proteoglycans and glycosaminoglycans (GAGs). I
first homogenized cartilage into powders by freeze-milling. I then tried
guanidine and phenol to dissolve the cartilage powder but it was not quite
efficient. Most of the 100mg of cartilage powder was not dissolved in 2 ml
guanidine/phenol (Qiagen tissue lysis reagent).
The other question is that RNA and GAGs are both negatively charged polymers
... 阅读全帖 |
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s****a
发帖数: 454 | 5 Thank you so much for replying.
I will add CHX in my homogenization.Will 100ug/ml work?
I wondered whether the height of the peak is related to the sample size and
type? I used 30mg of mouse myocardium.
Shall I pool fractions from 11 to 19 and isolate RNA for RT-PCR? |
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h*******o
发帖数: 4884 | 6 For Isolation RNA from brain tissues, you need to choose RNA kits that are
specific for fat-rich tissues, regular kits do not work well. |
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b*******0
发帖数: 125 | 7 采用了一个SVF isolation的方法，分离脂肪中的SVF，可是总是失败。分离的SVF要么不着板，要么死光光
。求支招。 |
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w**f
发帖数: 38 | 8 To define MMTV-Cre expression pattern, I crossed MMTV-Cre mice with Rosa-YFP
reporter mice. Very nice GFP fluorescence signal was seen in ductal trees
of Cre-postive/Rosa-YFP (+/-) mouse mammary glands. I then isolated mammary
epithelial cells using the Stem Cell kit (Collagenase/Hyaluronidase
digestion). No GFP-positive cells were detected using FACS analysis. Can
anyone give some suggestions on how to preserve GFP for FACS? Or can anyone
recommend one good protocol to cut sections from frozen ... 阅读全帖 |
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发帖数: 1 | 9 www.sigmaaldrich.com/catalog/product/sigma/nuc101
Nuclei Isolation Kit: Nuclei EZ Prep
到处都找不到recipe. 想自己配点，实在不想买（到货太慢）。 |
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发帖数: 1 | 10 没错，我看了你贴的poster，确实就是NP40（也叫Igepel CA630）。
Sigma坑人啊，这个buffer卖的不便宜。
Sigma的客服回信说
Hello
Thank you for contacting Sigma-Aldrich Technical Service, now MilliporeSigma
- the life science business of Merck KGaA, Darmstadt, Germany.
The exact formulation of the lysis buffer in the NUC101 - Nuclei Isolation
Kit: Nuclei EZ Prep is withheld as proprietary information. We can say that
it is a non-ionic detergent that does not contain reducing agents. And it
does not contain Triton or any protease inhibitors... 阅读全帖 |
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M*******C
发帖数: 183 | 11 想从病人blood里分离T cell，大家有什么推荐？谢谢
Stem cell公司的人跟我推荐了 EasySep™ Direct Human T Cell Isolation Kit。 |
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u**********e
发帖数: 26 | 12 看你收集T细胞后面要干嘛，
如果不介意activate T细胞，用CD3 beads就行。否则就negative selection。
miltenyi和Stemcell Tech都有Pan T Cell Isolation Kit。 |
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s****y
发帖数: 62 | 13 请假大家一个问题
SERS用Au做substrate是利用nano金表面粗糙分布来增强信号。
所以它的顺序是 金的地盘，上面有target analyte。化合物是放在金的上面。
在2010年厦大一个教授发表在nature上面一个文章，叫shell isolate nanoparticle
enhanced Raman spectroscopy.
他们做的是一种金的nano颗粒，然后撒在表面，用来增强Raman信号。我想请教一下，
这个方法，金颗粒是撒在化合物的上面还是在下面？如果是在化合物的上面，那他是利
用什么原理增强信号的？
这个是nature上面的那个文章的link ：
http://www.nature.com/nature/journal/v464/n7287/full/nature0890
有兴趣的同学可以看一下~做Raman的专家也请大概看一下，然后帮忙解释一下，可以吗？
谢谢各位的帮忙。 |
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w*******y
发帖数: 60932 | 14 Noise Isolating In Ear Earphone, Headphone, Earbud for MP3 MP4 iPod PSP NDS
, 3.5mm, Dark Blue $0.69 (Item#254-136-001) $0.69 w/Coupon Code:
MLCK254136081371NL1 For First 1000 Orders
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w*******y
发帖数: 60932 | 16 New Altec Lansing MHP126 MUZX Series 3.5mm In-Ear Earphones, Comfortable
SnugFit, Noise-Isolating, Powerful 9 mm Neodymium Speakers
LINK:
http://www.dailysteals.com/
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w*******y
发帖数: 60932 | 20 Airlink101 Wireless 11N Mini USB Adapter for PC and MAC, Penny Size 802.11n
Draft 2.0 Adapter, 300Mbps Downloading, AWLL6075, 1T2R, Windows 7 64bit and
32bit - $9.99
Coupon Code :MLCK2162440 92742NL1 Starts On : 9/27/2010 5:00:00 AM limit
300 uses
Link:
http://www.meritline.com/airlink101-awll6075-golden-n-wireless-mini-usb-adapter---p-38742.aspx
Noise Isolating In Ear Earphone, Headphone, Earbud for MP3 MP4 iPod PSP NDS,
3.5mm, Black with White, 0843 - $0.69
Coupon Code :MLCK2541720 92774NL |
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w*******y
发帖数: 60932 | 21 For those who missed the $61 dollar deal last month, this is back. Ultimate
Ears SuperFi 5vi Noise Isolating Earphones w/ Mic. $64.99 @Amazon FSSS. Link
Below. I know it is 4 dollars more expensive but still a great deal. The
SuperFi 5's without mic is $95.50
LINK TO AMAZON:
http://www.amazon.com/gp/product/B001DERZK0/ref=oss_product
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w*******y
发帖数: 60932 | 22 FREE! 2-Pack: Plantronics MHS213 Noise Isolating Stereo Mobile Headset With
Microphone
Link:
http://1saleaday.com/
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w*******y
发帖数: 60932 | 24 3-Pack: Ace Noise Isolating Comfort Wear Earbud Headphones!
LINK:
http://www.bensoutlet.com/products/ace-headphones
The Rolling Stones, Your Father Burping, Rhianna, Loud Construction Next-
Door, Katy Perry, Your Grandma Farting, Keith Urban, Your Sister Whining...
There are some things you just don't want to listen to, and there are some
things that you'd love to listen to. With these noise reducing ear buds, you
can finally block out the crap you don't want to hear- like your father
burping af... 阅读全帖 |
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w*******y
发帖数: 60932 | 27 This my first post so please be kind
I found this deal on another deal site as I was searching for a code to
purchase this particular protein. This is very good quality protein, tastes
great and mixes easily.
Link:
http://www.americanpurewhey.com/whey-isolate-01.html
Example:
Add 50 lbs for $269.99, use coupon BLACK30 for 30% off.
Total is $194.98 shipped, or $3.89 per lb.
$269.99 - 30% ($81.00) + $5.99 (shipping) = $194.98
black20 works on purchases of $60 or more
black30 works on purchases o... 阅读全帖 |
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w*******y
发帖数: 60932 | 31 Tigerdirect has Klipsch 1012134 ProMedia High-Performance All-Media Noise
Isolating In-ear Headphones - Microphone, iPhone Compatible for 59.99 use
coupon code OKL16588 to get $30 off. Standard Shipping is $1.99. Tax applies
in few states.
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w*******y
发帖数: 60932 | 34 Logitech
Logitech Ultimate Ears SuperFi 4 Noise-Isolating Earphones
$29.99 with code superfi4_22511
free shipping
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w*******y
发帖数: 60932 | 36 Newegg
back again if you missed out on last times deal
Meelectronics M9P-BK Noise-Isolating 3.5mm Headset with Control talk (black)
= $10 with code EMCKGKB97
free shipping
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w*******y
发帖数: 60932 | 37 MEElectronics - M6 Stylish Sound-Isolating Sports Premium Headphones - 2
Pack (M6-VL-MEE) for $79.99 - $60.00 coupon code: MEEHDP430 (Exp. 05/06) = $
19.99 plus Free Shipping. No sales tax in most states.
COUPON CODE:
MEEHDP430
ENJOY!
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w*******y
发帖数: 60932 | 40 Newegg
If you missed these a little over a month ago here, they are back!
2-pack MEElectronics M6 Sound-Isolating Sports Premium Headphones
= $20 with free shipping
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