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全部话题 - 话题: induce
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r*****b
发帖数: 310
1
First solar is going to produce PV-module in the second half of 2014. It
will have chance to catch up with the distributed installation market.
The First Solar TetraSun™ Module is design ned for applications where
space is at a premium or customers prefer a high-power density solution.
With a proprietary cell architecture and manufacturing process, our
crystalline modules offer one of the industry’s highest power ratings and
lowest temperature coefficients, resulting in superior energy den... 阅读全帖
L******r
发帖数: 141
2
来自主题: Immigration版 - 怎样写好推荐信 EB1A/B
All the materials were taken from Administrative Decisions (Aliens with Extraordinary Ability/ Outstanding Professors and Researchers). 所以这些话是权威!!!
I downloaded All the AAO decisions for the past three years. I read one by one for a total number of more than 200 decisions. I took good paragraphs from recommendation letters which IOs thought have met criterion of contribution. Because the works were from PDF files, I had to reformat them into word file.
It took me many hours to do the work and I... 阅读全帖
O*C
发帖数: 649
3
来自主题: Immigration版 - (gone) Med chem review opportunity
好像不太适合你啊。。。。
Abstract在这里,做med chem或者类似的给我pm吧
Beneficial effects of n-3 polyunsaturated fatty acids (PUFAs) on psoriasis
have been reported in rats, mice, humans, but the specific mechanisms
involved have not been well clarified. This study used the fat-1 mouse, a
transgenic model that endogenously convert n-3 PUFAs from n-6 FA to directly
determine if outcomes of psoriasis were correlated with n-3 PUFAs. Wild
type (WT) and fat-1 mice, which were treated daily with Imiquimod (IMQ)
cream or contro... 阅读全帖
i********n
发帖数: 171
4
来自主题: Immigration版 - 生物医学审稿人
请发简历以及相应的审稿文章编号到[email protected]/* */
1. Possible Contributions of Thyroid Hormone Replacement to Specific
Behaviors of Cancer
2. ATTENUATING EFFECT OF GYMNEMA SYLVESTRE ON STREPTOZOTOCIN-NICOTINAMIDE
INDUCED DIABETIC NEPHROPATHY IN RATS
3. Estrogen and Thyroid Cancer is a stem affair. A preliminary study
4. Therapeutic outcomes of autologous CIK cells as a maintenance therapy
in the treatment of lung cancer patients: A retrospective study
5. Role of gastrointestinal motili... 阅读全帖
s**u
发帖数: 9035
5
发信人: shmu (shmu), 信区: FDU
标 题: 一复旦校友从著名的Lab海归杭州师范大学副教授
发信站: BBS 未名空间站 (Sun Oct 21 09:15:12 2012, 美东)
我们有个校友,1998年硕士毕业留校,2001年来美国,2006年Tufts University Ph.D.
2010年海归杭州师范大学副教授。
zhi Lian directly
View Full Profile
Fuzhi Lian's Overview
Current
Associate Professor at Hangzhou Normal University
Past
Scientist III at USDA Human Nutrition Research Center on Aging at
Tufts University
Postdoctoral Associate at USDA Human Nutrition Research Center on
Aging at Tufts University
... 阅读全帖
s**u
发帖数: 9035
6
发信人: shmu (shmu), 信区: FDU
标 题: 一复旦校友从著名的Lab海归杭州师范大学副教授
发信站: BBS 未名空间站 (Sun Oct 21 09:15:12 2012, 美东)
我们有个校友,1998年硕士毕业留校,2001年来美国,2006年Tufts University Ph.D.
2010年海归杭州师范大学副教授。
zhi Lian directly
View Full Profile
Fuzhi Lian's Overview
Current
Associate Professor at Hangzhou Normal University
Past
Scientist III at USDA Human Nutrition Research Center on Aging at
Tufts University
Postdoctoral Associate at USDA Human Nutrition Research Center on
Aging at Tufts University
... 阅读全帖
n*******y
发帖数: 3337
7
【 以下文字转载自 TexasHoldem 讨论区 】
发信人: cmis91 (dontknow), 信区: TexasHoldem
标 题: 我是如何拿下Poker Stars周日百万赛冠军的
关键字: PokerStars SundayMillion 8202名参赛者 冠军$246K
发信站: BBS 未名空间站 (Sat Nov 13 15:03:46 2010, 美东)
(pic. provided by fryking, lol)
First briefly describe the course how I reach the final table. At the very
beginning of tourney, I got double up over someone’s donkey move. After
that, my AA 3 bet preflop, villain call and hit bottom two pairs, we went
all in and fortunately I catched higher two pairs at r... 阅读全帖
f********t
发帖数: 66
8
欧洲一个杂志
我们学校的图书馆没有这个的全文
美国只有Indiana U of Pennsylvania, Princeton U, U of Southern California 有
全文
如果有愿意帮忙的同学,非常感谢您
如果可以的话,请您将paper发到我的邮箱 t***********[email protected]
http://europace.oxfordjournals.org/content/early/2013/11/04/eur
Drug-induced long-QT syndrome and torsade de pointes: an underrated problem?
Prashanth Rao and
Peter R. Kowey*
Drugs that cause an acquired disorder of myocardial repolarization with a
characteristic prolongation of the QT interval on electrocardiogram (ECG)
are numerous and ... 阅读全帖
S******s
发帖数: 5376
9
【 以下文字转载自 TexasHoldem 讨论区 】
发信人: cmis91 (dontknow), 信区: TexasHoldem
标 题: 我是如何拿下Poker Stars周日百万赛冠军的
关键字: PokerStars SundayMillion 8202名参赛者 冠军$246K
发信站: BBS 未名空间站 (Sat Nov 13 15:03:46 2010, 美东)
(pic. provided by fryking, lol)
First briefly describe the course how I reach the final table. At the very
beginning of tourney, I got double up over someone’s donkey move. After
that, my AA 3 bet preflop, villain call and hit bottom two pairs, we went
all in and fortunately I catched higher two pairs at r... 阅读全帖
p***r
发帖数: 20570
10
来自主题: Bridge版 - 怎么打?
That's actually right. Sometimes bad bids or defense may induce bad bids or
defense from opps in the future. In that sense, it is very profitable to
make small mistakes to induce large mistakes from opps, which can be more
profitable than the strategy that is mistake free, but also quite
predictable. This kind of philosophy is applied in no limit holdem a lot.
Many very good players just make a lot of small mistakes and try to induce a
huge one from their inexperienced opps. Still, against such ... 阅读全帖
b*****n
发帖数: 6324
11
来自主题: Fitness版 - 练出高潮来! (转载)
【 以下文字转载自 Sex 讨论区 】
发信人: PharmD (夜里发呆), 信区: Sex
标 题: 练出高潮来!
发信站: BBS 未名空间站 (Thu Mar 22 12:06:39 2012, 美东)
http://www.guokr.com/article/123804/
练出高潮来!
Big.D 2012-03-21 21:33:37
众所周知,刺激阴蒂或者进行阴道性交都能够让女性达到高潮。不过,最近印第安纳大
学性学专家赫本尼克(Debby Herbenick)和福坦巴里教授(J. Dennis Fortenberry)
等人的发现却告诉我们,一些女性可能在健身的过程中,也会感受到高潮。研究者暂时
把这种高潮称为“核心高潮”(coregasm),因为这种高潮与核心肌群训练密切相关,
比如攀爬、骑车和举重。(编者按,核心肌群大体而言相当于腹背部的肌肉群,主要功
能为维持脊椎稳定)
赫本尼克和她的团队通过网络问卷的方式,调查了总计530位女性,其中有124位报告在
锻炼过程中体验到高潮(EIO,exercise-induced orgasm),246位报告在锻炼过程中
体... 阅读全帖
h*t
发帖数: 42
12
来自主题: loseweight版 - 推荐一下CLA
更多文献,有兴趣看看?
Peer-Reviewed Research References
Bosello, O., & Zamboni, M. (2000). Visceral obesity and metabolic syndro
me.Obesity reviews, 1(1), 47-56.
Merat, S., Casanada, F., Sutphin, M., Palinski, W., & Reaven, P. D. (199
9). Western-type diets induce insulin resistance and hyperinsulinemia in LDL
receptor-deficient mice but do not increase aortic atherosclerosis compared
with normoinsulinemic mice in which similar plasma cholesterol levels are a
chieved by a fructose-rich diet. Arter... 阅读全帖
G****e
发帖数: 11198
13
来自主题: NCAA版 - 有关Booster的NCAA规定
从密歇根大学网站上拷贝来的:
http://www.mgoblue.com/compliance/mich-compliance-whoisabooster
*******************************
Q: Once an individual has been identified as a "booster", how long does he
or she retain this identity
A: Forever.
*******************************
NCAA Bylaw 13.02.11 defines "booster". In part, this regulation states:
"A booster (i.e., representative of the institution's athletics interests)
is an individual, independent agency, corporate entity (e.g. apparel or
equipment manufacturer)... 阅读全帖
b***i
发帖数: 10018
14
来自主题: Running版 - interesting post: 生命在于运动
http://www.letsrun.com/forum/flat_read.php?thread=4519027&page=
Looking the posts from jaguar1:
I did my Master's thesis on enhancing bone recovery with whole body
vibration training-- basically a low-magnitude, high frequency stimulus. You
could equate this to ~walking, standing, low-intensity running. Low
intensity activity is both safer and more anabolic to both soft and hard
tissue. It's also more likely to be sustained long term and consistently--
it's what maintains bone/muscle mass. When ... 阅读全帖
g*2
发帖数: 658
15
来自主题: Running版 - 哮喘病史跑步新手的问题
楼主的可能是运动引起的支气管收缩
Exercise-induced bronchoconstriction (EIB) refers to the episodic
bronchoconstriction that follows exercise in many asthmatic patients. This
reflects the view that exercise is a trigger of bronchoconstriction in
patients with underlying asthma.
坚持运动是好的,因为这样可以增强心肺功能。 但要避免在太冷,太干的环境中运动
Improving a patient's cardiovascular fitness reduces the minute ventilation
required for a given level of exercise, thereby decreasing the stimulus for
bronchoconstriction. Avoiding exercise in cold,... 阅读全帖
c****1
发帖数: 457
16
(pic. provided by fryking, lol)
First briefly describe the course how I reach the final table. At the very
beginning of tourney, I got double up over someone’s donkey move. After
that, my AA 3 bet preflop, villain call and hit bottom two pairs, we went
all in and fortunately I catched higher two pairs at river and suck him out.
With 3X starting chips, I cruised all the way with healthy chips until
around 100 players remain when the blinds increase faster than the chips I
can accumulate. After my... 阅读全帖
h*******s
发帖数: 3932
17
来自主题: TexasHoldem版 - [合集] 讨论一手以前打的live 牌
☆─────────────────────────────────────☆
player (Hope is the best thing) 于 (Mon Nov 21 15:26:29 2011, 美东) 提到:
5/10 NL. Very loose game and lots of action. A few guys sat down with 10+
K.
I played a super nitty game with shortstack(starting with 1k) at the table
and tried to not getting involved with many pots.
Then this hand came out: I was at SB with 1.8k, there were two limpers,
including button with very big stack. I looked my hand and I got KK. So I
made 100$ to go out of SB, B... 阅读全帖
N*********6
发帖数: 2302
18
【 以下文字转载自 Biology 讨论区 】
发信人: Narcissus26 (Jane), 信区: Biology
标 题: 帮师弟寻找一个postdoc 位置,谢谢
发信站: BBS 未名空间站 (Tue Dec 29 08:56:37 2015, 美东)
人在国内,有11篇文章。有兴趣的PI, 请email me ::[email protected]
/* */
谢谢!
1. Kang H, Cao S, Chen T, Jiang Z, Liu Z, Li Z, Xu Q, Lin Q, WeiS. The poor
recovery of neuromyelitis optica spectrum disorder is associated with a
lower level of CXCL12 in the human brain. J Neuroimm, 289:56-61, 2015.(co-
first author)
2. Chen T, Li Z, Jiang Z, Liu Z, Xu Q, Huang D, Lin Q, Wei.S (... 阅读全帖
n*******y
发帖数: 3337
19
【 以下文字转载自 TexasHoldem 讨论区 】
发信人: cmis91 (dontknow), 信区: TexasHoldem
标 题: 我是如何拿下Poker Stars周日百万赛冠军的
关键字: PokerStars SundayMillion 8202名参赛者 冠军$246K
发信站: BBS 未名空间站 (Sat Nov 13 15:03:46 2010, 美东)
(pic. provided by fryking, lol)
First briefly describe the course how I reach the final table. At the very
beginning of tourney, I got double up over someone’s donkey move. After
that, my AA 3 bet preflop, villain call and hit bottom two pairs, we went
all in and fortunately I catched higher two pairs at r... 阅读全帖
t******k
发帖数: 553
20
我不发表对二人是否结婚事情的任何意见,也不想知道,这个世界为博出位出名,手段多了去了。
裴院士,以前听过报告,有所认识。对于谢欣,这位最年轻的973首席科学家,海龟博士。开红色Audi TT的973兽吸,我还是第一次听说,无聊间,google了一下。
以下是转帖
引用:
这里就是一个学术造诣颇深的院士和一个混迹学术界想要出头的高干千金的两厢情愿的合作。
我们先来看一下一个手里控制着上亿元科研经费的中国最年轻的973首席科学家,973胚
胎发育干细胞项目首席科学家的基本情况:
谢欣研究员, 现任中国财政部长谢旭人之女
现任 中国科学院上海药物所 研究员、 国家新药筛选中心模型建立 II 部主管、 课题
组长 。
下面是她所有的有名字的文章,还算诚实,没用同名同姓的凑数。
可是大家已经要问这样的条件,没有一篇CNS系列的文章,1st-authored paper只有3篇
,够中科院研究员、百人计划择优资助吗?
那些录用的评委和百人计划的专家组,都糊涂了吗?这么一反以“严格”著称之常态了
呢?
1.Chen D, Liao J, Li N, Zhou C, Liu Q, Wang G, Zh... 阅读全帖
p********3
发帖数: 5750
21
50, 70, 71; 大家注意一下和这几篇文献相关的话题。 另外, 73-75我本人没有看,确实是谈的印度药材, 我在查阅参考文献的时候在搜索Chinese Medicine的时候, 显示有这些文献,我本人没有亲自看这些文献, 也许其中有讲中药的地方。不过这不影响我要表达的意思。
慢慢看:
INTRODUCTION — Plants have been used for medicinal purposes for thousands
of years. All major cultures including Native American, European, South
American, Asian, and African cultures have used botanicals for healing
purposes. As an example, saw palmetto was used for urinary symptoms in men
from Egypt in the 15th century BC [1]. Hippocrates documented... 阅读全帖
s**u
发帖数: 9035
22
发信人: shmu (shmu), 信区: FDU
标 题: 一复旦校友从著名的Lab海归杭州师范大学副教授
发信站: BBS 未名空间站 (Sun Oct 21 09:15:12 2012, 美东)
我们有个校友,1998年硕士毕业留校,2001年来美国,2006年Tufts University Ph.D.
2010年海归杭州师范大学副教授。
zhi Lian directly
View Full Profile
Fuzhi Lian's Overview
Current
Associate Professor at Hangzhou Normal University
Past
Scientist III at USDA Human Nutrition Research Center on Aging at
Tufts University
Postdoctoral Associate at USDA Human Nutrition Research Center on
Aging at Tufts University
... 阅读全帖
s**u
发帖数: 9035
23
发信人: shmu (shmu), 信区: FDU
标 题: 一复旦校友从著名的Lab海归杭州师范大学副教授
发信站: BBS 未名空间站 (Sun Oct 21 09:15:12 2012, 美东)
我们有个校友,1998年硕士毕业留校,2001年来美国,2006年Tufts University Ph.D.
2010年海归杭州师范大学副教授。
zhi Lian directly
View Full Profile
Fuzhi Lian's Overview
Current
Associate Professor at Hangzhou Normal University
Past
Scientist III at USDA Human Nutrition Research Center on Aging at
Tufts University
Postdoctoral Associate at USDA Human Nutrition Research Center on
Aging at Tufts University
... 阅读全帖
n**e
发帖数: 2026
24
今年初,美国科学促进会(AAAS)和皮尤研究中心(Pew Research Center)联合公布
的一项调查显示,88%的科学家认为转基因食品可安全食用,持这一看法的公众只有37
%。报告认为差距如此之大的一个可能原因是,三分之二的公众认为科学家对转基因食
品的健康后果没有清晰认识。这就是真正的原因吗?
根据报告全文,皮尤中心给19,984名科学家发出了调查问卷,3748人(18.8%)完成了
问卷调查。其中生物医学学科背景的科学家占50%。报告没有说明50%的生物医学背景是
否能够代表科学家总体的学科构成。更大问题是在这50%的科学家人群中,有多少人的
工作是与转基因作物的研究有关,以什么样的方式相关。报告没有提供任何信息。
由于这些关键信息缺失,88%的科学家相信转基因安全的说法毫无意义。在一个没有代
表性并且无法排除偏向性的样本中调查,科学家相信转基因安全从1%到99%与88%没有任
何不同。好消息是超过半数的美国民众认为转基因不安全。
2012年10月,美国科学发展协会以官方名义(Board of Directors )抛出了一个反对
转基因标识声明(附录1)。目的就是诱导民... 阅读全帖
r******t
发帖数: 8967
25
【 以下文字转载自 Biology 讨论区 】
发信人: xiaomingxiao (xiaomingxiao), 信区: Biology
标 题: 气功大师发表的SCI,牛啊
发信站: BBS 未名空间站 (Mon Aug 5 15:48:20 2013, 美东)
Cell Physiol Biochem. 2013;31(1):113-22. doi: 10.1159/000343354. Epub 2013
Jan 25.
External Qi of Yan Xin Qigong inhibits activation of Akt, Erk1/2 and NF-&#
312;B and induces cell cycle arrest and apoptosis in colorectal cancer cells.
Yan X, Shen H, Jiang H, Hu D, Wang J, Wu X.
Background/Aims: Colorectal cancer (CRC) is the second leading cause of
cancer death... 阅读全帖
w*********r
发帖数: 3382
26
来自主题: TrustInJesus版 - 我相信沒有神(譯)
you are distorting words
i am not mutation expert but here is some of my humble opinion on this
subject
mutation can be spontaneous mutation, or induced mutation. what we are
talking about here is presumably spontaneous mutation that has been happening throughout the history of evolution
spontaneous mutation can be random, and the reason we think so is that so far scientists did not see a pattern of spontaneous mutation occurring, or any evidence that supports that spontaneous mutation happens i... 阅读全帖
J******s
发帖数: 7538
27
The repetitive training of a certain neuronal pathway will strengthen it and
induce some changes on protein level. However this should be different
with the changes of muscles induced by fitness practicing.
Only through non-repetitive 禅修, the detectable changes on protein level
can not happen. I think the structual changes in central/peripheral neural
system mostly happen in the synapses.
Even the structural change is induced by 禅修, the utilization of it still
needs some stimuli (under certain ... 阅读全帖
J******s
发帖数: 7538
28
The repetitive training of a certain neuronal pathway will strengthen it and
induce some changes on protein level. However this should be different
with the changes of muscles induced by fitness practicing.
Only through non-repetitive 禅修, the detectable changes on protein level
can not happen. I think the structual changes in central/peripheral neural
system mostly happen in the synapses.
Even the structural change is induced by 禅修, the utilization of it still
needs some stimuli (under certain ... 阅读全帖
Y**u
发帖数: 5466
29
☆─────────────────────────────────────☆
wuyu (wuyu) 于 (Fri Sep 9 12:23:52 2011, 美东) 提到:
行禅在念住修行中有很重要的地位。跟大多数这儿的同学们一样,我没有福气有专门时
间在一个清静的地方做行禅。只能在上班路上做行禅,虽然效果不是很好,但千里之行
,始于脚下,希望为将来有机会密集禅修打下基础。
我曾经试着给每一步脚掌的移动分解标识“按,移,推,提”。开始感觉效果不错
,但缺点是标识经常跟不上所缘变化,导致标识变成了念口诀。后来请教卡玛兰蒂禅师
,她建议我只用察觉“左脚,右脚”。用这个方法我基本上能跟上所缘变化。但是很容
易走神被杂念打乱。到最后,我想了一个办法。这个办法跟我练习安般息念的经验有关
,我一般用数息(吸呼1-10)来使自己沉淀杂念。在行禅中,我也试着以“数步”让自
己能持续专注。我发现数七步是最好的:(一)容易持续专注;跟数息的道理一样。(
二)可进可退;如果环境嘈杂,或专注力弱,可只觉知“一三五七”步。如果专注力强
,可以“一二三四五六七”左右脚全部觉知(三)易于平衡;因为是奇... 阅读全帖
s**u
发帖数: 9035
30
我们有个校友,1998年硕士毕业留校,2001年来美国,2006年Tufts University Ph.D.
2010年海归杭州师范大学副教授。
zhi Lian directly
View Full Profile
Fuzhi Lian's Overview
Current
Associate Professor at Hangzhou Normal University
Past
Scientist III at USDA Human Nutrition Research Center on Aging at
Tufts University
Postdoctoral Associate at USDA Human Nutrition Research Center on
Aging at Tufts University
Research Associate (助教) at Fudan University
Education
Tufts University
Fudan U... 阅读全帖
s**u
发帖数: 9035
31
我们有个校友,1998年硕士毕业留校,2001年来美国,2006年Tufts University Ph.D.
2010年海归杭州师范大学副教授。
zhi Lian directly
View Full Profile
Fuzhi Lian's Overview
Current
Associate Professor at Hangzhou Normal University
Past
Scientist III at USDA Human Nutrition Research Center on Aging at
Tufts University
Postdoctoral Associate at USDA Human Nutrition Research Center on
Aging at Tufts University
Research Associate (助教) at Fudan University
Education
Tufts University
Fudan U... 阅读全帖
s**u
发帖数: 9035
32
发信人: shmu (shmu), 信区: FDU
标 题: 一复旦校友从著名的Lab海归杭州师范大学副教授
发信站: BBS 未名空间站 (Sun Oct 21 09:15:12 2012, 美东)
我们有个校友,1998年硕士毕业留校,2001年来美国,2006年Tufts University Ph.D.
2010年海归杭州师范大学副教授。
zhi Lian directly
View Full Profile
Fuzhi Lian's Overview
Current
Associate Professor at Hangzhou Normal University
Past
Scientist III at USDA Human Nutrition Research Center on Aging at
Tufts University
Postdoctoral Associate at USDA Human Nutrition Research Center on
Aging at Tufts University
... 阅读全帖
s**u
发帖数: 9035
33
发信人: shmu (shmu), 信区: FDU
标 题: 一复旦校友从著名的Lab海归杭州师范大学副教授
发信站: BBS 未名空间站 (Sun Oct 21 09:15:12 2012, 美东)
我们有个校友,1998年硕士毕业留校,2001年来美国,2006年Tufts University Ph.D.
2010年海归杭州师范大学副教授。
zhi Lian directly
View Full Profile
Fuzhi Lian's Overview
Current
Associate Professor at Hangzhou Normal University
Past
Scientist III at USDA Human Nutrition Research Center on Aging at
Tufts University
Postdoctoral Associate at USDA Human Nutrition Research Center on
Aging at Tufts University
... 阅读全帖
G**s
发帖数: 1148
34
还有很多review, cell, neuron和其他杂志上的paper.
看起来很牛压
Calcium signaling in the guidance of nerve growth by
netrin-1. [K. Hong, M. Nishiyama, J. Henley, M.
Tessier-Lavigne, and M-m. Poo (2000) Nature 403, 93-98]
Gating of BDNF-induced synaptic potentiation by cAMP. [L
Boulanger, and M-m. Poo (1999) Science 284,1982-1984]
Distributed synaptic modification in neural networks induced
by patterned stimulation. [G. Bi and M-m. Poo (1999) Nature
401, 792-796]
Activity-induced potentiation of developing neuromus
a***a
发帖数: 40617
35
来自主题: Biology版 - 我是不是吃饱了撑的?
pGEX?
本来这个yield就不高,我每次为了测个序,都至少要从10ml overnight culture里小抽
有关不表达的问题,建议你所有clone(一个construct做8~12个),先煮菌,然后挑阳性的
用1ml small scale 测试表达情况,简单做法是
overnight culture,1:100稀释到1ml LB中,37度,3hr,然后用终浓度0.2~1mMIPTG
induce
37度3hr,然后取100ul,离心,扔掉90ul上清,加10ul 2xSDS loading buffer,不用重悬
直接煮10min,跑胶
有的时候这种条件可能表达时间不够看不出来表达,就要用更麻烦的small scale
就是2ml体系,其他都一样,但是要37度养到od 0.3,然后换18度养到od 0.6,再induce
induce后用18度,200rpm,overnight(8~16hr),然后再看whole cell蛋白看有没有表达
如果上面两个都没表达,基本上就没啥希望了
x********u
发帖数: 430
36
来自主题: Biology版 - IPTG problem
We use T7 promoter for our vector system. We have been very successful to
produce the recombinant protein for bioconversion. Usually we induce the
cell with IPTG for 3-4 hours before bioconversion. But this time I induce
the cell for about 12 hours and I didn't get any products. The cell grows
well and seems normal. What's the consequence if you induce the cell too
long with IPTG? Does this matter with my expression results? Thank you!
S**********e
发帖数: 1789
37
看了这个教授的主页,竟然公布了自己review的文章,题目,杂志名称都有,是不是违反职业道德呢?
Editorial Activities
Journal Peer Reviewer
2009 International Journal of Cancer. (Article) E-cadgerin
transcriptional down-regulation by epigenetic and microRNA-200 family
alterations is related to mesenchymal and drug-resistant phenotypes in human
breast cancer cells, IJC-09-1835.
2009 Molecular Cancer Therapeutics. (Article) Role of reactive oxygen
species in the ability of H-Ras to enhance cell death induced by histone
deacetylase inhibit... 阅读全帖
g*********d
发帖数: 233
38
人们都知道锻炼身体可以促进新陈代谢,有益于心血管健康,但此前科学家对运动究竟如何影响心脏
却所知甚少。美国哈佛大学医学院的研究人员日前报告说,他们首次从分子水平发现运动有益心脏健
康的机理,这一发现将有助于开发出治疗心血管疾病的新疗法。
研究人员通过小鼠实验发现,经常运动可以使小鼠体内的C/EBPβ转录因子水平显著下降,其结果会促
进小鼠心脏肌肉细胞增殖,有益于心脏生长。此外,研究人员还发现,体内C/EBPβ水平较低的小鼠对
心力衰竭具有抵抗能力。
研究人员表示,这项研究对心脏肌肉再生的潜力有了深入理解。参与研究的哈佛大学医学院教授安东
尼·罗森茨魏希表示,通过这项研究可以开发出针对那些无法运动的心脏病患者的疗法。
这项研究成果发表在新一期美国《细胞》杂志上。
C/EBPβ Controls Exercise-Induced Cardiac Growth and Protects against
Pathological Cardiac Remodeling
Cell, Volume 143, Issue 7, 1072-1083, 23 December 2010
10.1016/j... 阅读全帖
m***f
发帖数: 1622
39
来自主题: Biology版 - NIBS的高绍荣是怎么tenure的?

扯淡呢,看看高最近两年发表的文章吧
CELL STEM CELL, JBC, STEM CELL, PNAS,都是很不错的
就这样还不能PROMOTION? 在美国都可以tennure了
1. Wu T., Wang H., He J., Kang L., Jiang Y., Liu J., Zhang Y., Kou
Z., Liu L., Zhang X., Gao S. (2011) Reprogramming of trophoblast stem
cells into pluripotent stem cells by Oct4. Stem Cells 2011 Feb 8.
[Epub ahead of print].
2. Kang L., Wu T., Tao Y., Yuan Y., He J., Zhang Y., Luo T., Kou Z.,
Gao S. (2010) Viable mice produced from 3-factor induced pluripotent
stem (iPS) cells through tetrapl... 阅读全帖
a*****x
发帖数: 901
40
这篇文章开头就有的:
“We have shown previously that embryonic stem cells (ESCs) and induced
pluripotent stem cells (iPSCs) from male (XY) mice are induced into epiblast
-like cells (EpiLCs), which are in turn induced into primordial germ cell–
like cells (PGCLCs) with proper function for spermatogenesis and offspring
production”
所以精子用的是XY

oocyte
p*****e
发帖数: 93
41
1.
CARMA3 deficiency abrogates G protein-coupled receptor-induced NF-κB
activation
Brian C. Grabiner1,5, Marzenna Blonska1,5, Pei-Chun Lin1, Yun You1, Donghai
Wang4, Jiyuan Sun1, Bryant G. Darnay2, Chen Dong3, and Xin Lin1,6
2.
MEKK3 is required for lysophosphatidic acid-induced NF-kappaB activation.
Sun W, Li H, Yu Y, Fan Y, Grabiner BC, Mao R, Ge N, Zhang H, Fu S, Lin X,
Yang J.
3.
Bcl10 plays a critical role in NF-κB activation induced by G protein-
coupled receptors
Donghai Wang * , † ... 阅读全帖
s**u
发帖数: 9035
42
来自主题: Biology版 - 谢博士去miami了啊
He has 12 papers, 3 of which are first-author papers. see below
Differential regulation of androgen receptor by PIM-1 kinases via
phosphorylation-dependent recruitment of distinct ubiquitin E3 ligases.
Linn DE, Yang X, Xie Y, Alfano A, Deshmukh D, Wang X, Shimelis H, Chen H, Li
W, Xu K, Chen M, Qiu Y.
J Biol Chem. 2012 Jun 29;287(27):22959-68. Epub 2012 May 14.
PMID:
22584579
[PubMed - indexed for MEDLINE]
Related citations
2.
A Role for OCT4 in Tumor Initiation of Drug-Resistant Prostat... 阅读全帖
s**u
发帖数: 9035
43
Dr.Xie has 12 papers, 3 of which are first-author papers. see below
Differential regulation of androgen receptor by PIM-1 kinases via
phosphorylation-dependent recruitment of distinct ubiquitin E3 ligases.
Linn DE, Yang X, Xie Y, Alfano A, Deshmukh D, Wang X, Shimelis H, Chen H, Li
W, Xu K, Chen M, Qiu Y.
J Biol Chem. 2012 Jun 29;287(27):22959-68. Epub 2012 May 14.
PMID:
22584579
[PubMed - indexed for MEDLINE]
Related citations
2.
A Role for OCT4 in Tumor Initiation of Drug-Resistant Pro... 阅读全帖
s**u
发帖数: 9035
44
发信人: shmu (shmu), 信区: FDU
标 题: 一复旦校友从著名的Lab海归杭州师范大学副教授
发信站: BBS 未名空间站 (Sun Oct 21 08:28:35 2012, 美东)
我们有个校友,1998年硕士毕业留校,2001年来美国,2006年Tufts University Ph.D.
2010年海归杭州师范大学副教授。
zhi Lian directly
View Full Profile
Fuzhi Lian's Overview
Current
Associate Professor at Hangzhou Normal University
Past
Scientist III at USDA Human Nutrition Research Center on Aging at
Tufts University
Postdoctoral Associate at USDA Human Nutrition Research Center on
Aging at Tufts University
... 阅读全帖
s**u
发帖数: 9035
45
发信人: shmu (shmu), 信区: FDU
标 题: 一复旦校友从著名的Lab海归杭州师范大学副教授
发信站: BBS 未名空间站 (Sun Oct 21 08:28:35 2012, 美东)
我们有个校友,1998年硕士毕业留校,2001年来美国,2006年Tufts University Ph.D.
2010年海归杭州师范大学副教授。
zhi Lian directly
View Full Profile
Fuzhi Lian's Overview
Current
Associate Professor at Hangzhou Normal University
Past
Scientist III at USDA Human Nutrition Research Center on Aging at
Tufts University
Postdoctoral Associate at USDA Human Nutrition Research Center on
Aging at Tufts University
... 阅读全帖
j*******g
发帖数: 10
46
来自主题: Biology版 - 求文献paper 一篇,谢了。
thanks a lot!
Current Neurovascular Research
ISSN (Print): 1567-2026
ISSN (Online): 1875-5739
VOLUME: 9
ISSUE: 1
DOI: 10.2174/156720212799297100
PDGF Receptor β Signaling in Pericytes Following Ischemic Brain Injury
Author(s): Koichi Arimura, Tetsuro Ago, Masahiro Kamouchi, Kuniyuki Nakamura
, Koji Ishitsuka, Junya Kuroda, Hiroshi Sugimori, Hiroaki Ooboshi, Tomio
Sasaki and Takanari Kitazono
Pages 1-9 (9)
Abstract:
Platelet derived growth factor (PDGF)-B plays a neuroprotective role in
brain dam... 阅读全帖
h*******o
发帖数: 4884
47
hyperpolarization eventually lead to inhibition of the ETC, which induces
oxidative stress by multiple mechanisms.
Oligomycin will not induce "sustained hyperpolarization".
Actually, I don't think any ETC inhibitor will induce sustained
hyperpolarization.
Usually, a collapse of membrane potential will happen after "maybe 30 mins
or even shorter" of OXPHOS inhibition.
f******g
发帖数: 1003
48
来自主题: Biology版 - 由拔火罐刮痧所想到的
http://www.merckmanuals.com/vet/pharmacology/anti-inflammatory_
&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Biochemical mediators released during inflammation intensify and propagate
the inflammatory response (see Anti-Inflammatory Agents: Actions of
Inflammatory MediatorsTables). These mediators are soluble, diffusible
molecules that can act locally and systemically. Mediators derived from
plasma include complement and complement-derived peptides and kinins.
Released via the classic or alte... 阅读全帖
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