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全部话题 - 话题: histology
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l*******y
发帖数: 284
1
Leaftaney 叶子同学step one 很啰嗦的经验教训贴-243
我的分数不高,也就是CMG的平均分243。 在整个备考过程中,从麦地里面获得很多信
息,从前辈的讲座的里面学习到很多东西,包括学习方法,一路上不断修正自己的学习
方法。今天刚拿到分数,虽然分不高,但是在版主大大的鼓励下,还是决定写下这个帖
子,希望能帮助到考step 1的战友。你们一定比我考的好。
一,背景
老毕业生,本科毕业时间2006年,中国MD,PhD,目前part time 工作。家有萌娃一枚
,现18个月。出国前一直从事妇产科方面的研究,在中国当了一年多妇产科医生。
二,复习时间轴
2013年4月-2014年2月开始认英文单词,你没看错,就是认英文单词,想当年本科不
都是中文教材么。因为当时还在全职做妇产科住院医生,3-4天值个24小时班,值班的
时候平均每天晚上起来带着实习生做宫外孕手术2-3个,大家都懂的,一晚上就在仰卧
起坐。每天复习时间半个小时-1个小时不等。其实我也想多看看书的,呜呜呜。
2014年2月到-2014年9月,我看完一遍kaplan notes(除pathology以外),一遍
... 阅读全帖
l*******y
发帖数: 284
2
Leaftaney 叶子同学step one 很啰嗦的经验教训贴-243
我的分数不高,也就是CMG的平均分243。 在整个备考过程中,从麦地里面获得很多信
息,从前辈的讲座的里面学习到很多东西,包括学习方法,一路上不断修正自己的学习
方法。今天刚拿到分数,虽然分不高,但是在版主大大的鼓励下,还是决定写下这个帖
子,希望能帮助到考step 1的战友。你们一定比我考的好。
一,背景
老毕业生,本科毕业时间2006年,中国MD,PhD,目前part time 工作。家有萌娃一枚
,现18个月。出国前一直从事妇产科方面的研究,在中国当了一年多妇产科医生。
二,复习时间轴
2013年4月-2014年2月开始认英文单词,你没看错,就是认英文单词,想当年本科不
都是中文教材么。因为当时还在全职做妇产科住院医生,3-4天值个24小时班,值班的
时候平均每天晚上起来带着实习生做宫外孕手术2-3个,大家都懂的,一晚上就在仰卧
起坐。每天复习时间半个小时-1个小时不等。其实我也想多看看书的,呜呜呜。
2014年2月到-2014年9月,我看完一遍kaplan notes(除pathology以外),一遍
... 阅读全帖
G*********y
发帖数: 100
3
来自主题: MedicalCareer版 - 有人有兴趣一起过一下uw step1吗?
形式:
要求参加的同学uw至少做完一遍,正确率80%以上。
比如每一个器官的题目会按如下进行分类,就是uw create test的时候右边的选项器官
,左边的选项算分科。就是先按右边的选,然后选左边的。这样子便于大家统一。从3
月3号开始每周五上课,晚上大概西部时间5:30pm (如果有同学需要调整可以告诉我,
不过这个是最早的开始时间)。
每个周二晚上我会给大家发一个每一个器官下面所有按右边科目顺序排列的题目列表。
大家可以参考一下,以防我们有漏的或者大家不够统一的地方。
比如:
GI
-Anatomy,
-BS,
-Biochemistry,
-Biostatistics ,
-Embryology,
-Genetics,
-Histology,
-Immunology,
-Microbiology
-Pathology
-Physiology
-Pathophysiology
-Pharmacology
我们周五上课的时候会提前分配一下,我会列一个schedule之前。比如
GI, anatomy, BS, biochemistry, Biostatistics , emb... 阅读全帖
r*******g
发帖数: 5495
4
肺癌的risk factors:
1. 80% of patients with Lung cancer of all histologic types are current or
former smokers
2. Second hand smoke
3. Asbestos exposure
4. Arsenic exposure
5. Mustard gas
6. Low level radon
7. Prior lung disease(COPD/TB)
8. Specific genes/genetic variants
x****y
发帖数: 1853
5
结构生物学和分子生物学最后都是为了解答蛋白质怎么跟细胞相互作用的问题。 只是
看问题的尺度不一样而已。 所有结构生物学的分析手段都基本上看死的结构,不是生
物体正常的环境。比如X-ray要求结晶,电镜要求冷冻, NMR要求泡在某种液体加强磁
场和同位素替换。这些实验要求都已经改变了蛋白质native的活性的状态。
退一万步,哪怕你得到活性的原子级别结构, 又怎么样? 你只能看着这个原子结构图
, 猜想这些原子那些键怎么去跟细胞相互作用。
Confocal是直接在细胞的尺度看相互作用。跟结构生物学相比,首先它看得是活的细胞
。 通过荧光label,你可以清楚看到哪些protein在哪个function. 这比原子结构重要。
在Confocal出来之前,很多医院都用TEM来做histology 和 pathology. Confocal出来
以后,很多TEM没人用了。因为尽管你有分辨率,但是你出来的信息不如confocal那样
直接解答生物学的问题。很多医院里的electron microscopists 还因此失业。我本人
做电子显微镜设计,看着不少电镜从医院里被de-commissio... 阅读全帖
F*******n
发帖数: 813
6
来自主题: Military版 - 人的情商幼儿园就能看出来了
我大多数读书都是靠group discussion.
我唯一单打独斗的是human histology,biochemistry,cell biology, population
genetics.
我可以自己关书本画出人体所有细胞和组织的图。。。
我有我自己的图谱。。。。
C*********4
发帖数: 40
7
帮朋友转发的,有兴趣的直接联系老板~
The position is for Research Technician I/II or Research Assistant,
depending on the level of education and experience of the applicant. My
laboratory is interested in kidney development and in understanding the
pathogenesis of polycystic kidney disease using mouse models. Specifically,
I am interested in learning how tubules are formed during embryogenesis. I
have started my laboratory at the University of Texas Southwestern Medical
Center this January 2011 and am looking to ... 阅读全帖
J****G
发帖数: 293
8
来自主题: Classified版 - 卖 Step1 书
Kaplan Notes 2010,和相对的录像(DVD)。从中国买的。
打印的 Goljan notes
Lange Pharmacolgy Flash Cards (in a box, 2nd edition)
BRS Cell Biology and Histology, 3rd edition
Clinical Microbiology made ridiculously simple, 3rd edition
BRS Behavioral Science, 2nd edition
Biochemistry,Lippicott's Illustrated Reviews, 2nd edition
Basic Histrology by Doulas F. Paulsen, 2nd edition
BRS Pathology
BRS Embryology
BRS Physiology
Lippincotts Illustrated Reviews Pharmacology, 2nd edition
Medical Microbiology &Immunology by Warre... 阅读全帖
h*w
发帖数: 134
9
来自主题: Faculty版 - optical imaging的前景如何 (转载)
一个好的技术最重要的是发现好的应用,而不是完全来源于技术本身。
Ben的文章在于它有很好的合作者。当时我们也shock一下,但是回过头一项,实际可以
做。 不过的有一个fulltime的做半年的imaigng processing。 实际上应用有限。
optical imaging 从临床上来说,设备便宜而且实时。 由于深度问题,内窥视一个重
要解决办法。
至于contrast optical imaigng, animal study 可能应用。 临床不太行。 OCT 的优
点就是 不要contrast , images look like histology. OCT是目前所有
opticalimaigng 中最有临床价值的。不是忽悠
Embryo imaging with OCT is its imaging speed (3D in real time).
海量数据不是问题。
s******y
发帖数: 28562
10
来自主题: Faculty版 - 是招俩technician还是招俩博后?
很多杂七杂八的项目可以让他做啊! 还有一些什么genotyping, histological
analysis,house keeping (prepare pipet, make medium and buffer)等等,
因为这些都是比较labor intense 但是又没有什么意思的常规事情,
一般博士后让他们做这些可能会抱怨的。
s******y
发帖数: 28562
11
一般都比较稳定。 尤其是特别重要的core (such as histology), 即使是资金紧张也
不会影响到的
P**********l
发帖数: 310
12
来自主题: Faculty版 - 周末挖坑:合适的伴侣
应个景:
Advice for a Young Investigator
Santiago Ramon y Cajal.
Although many would disagree, we believe that the man of science should be
married and should face the pressures and responsibilities of family life
courageously.
He will not emulate the selashness of Epicurus, who did not marry in an
attempt to avoid cares and woes, nor the exaggerated reanement of Napoleon,
whose only use for a woman was a nurse in old age.3 For the man of science,
the aid of a wife is just as necessary in youth as i... 阅读全帖
p*******r
发帖数: 156
13
Department of Otolaryngology, University of Kentucky Medical Center

A postdoctoral position is available immediately to study gap junctional
function in hearing. Gap junction connexin mutations are responsible for 70-
80% of genetic hearing loss in human, which is the highest incidence in
genetic diseases. Our ongoing projects are evaluating gap junctional
function and contributions to hearing in the inner ear and mechanisms
underlying gap junction deficiency induced hearing loss. Ongoing wor... 阅读全帖
t***a
发帖数: 45
14
来自主题: JobHunting版 - Histology technician needed ASAP
要人很急,机会很大,

therapeutic
t***a
发帖数: 45
15
来自主题: JobHunting版 - Histology technician needed ASAP
up up

therapeutic
s*******2
发帖数: 598
16
发信人: s19990602 (s19990602), 信区: MedicalCareer
标 题: 这种没有ASCP认证的证书班发的所谓证书有效吗?
发信站: BBS 未名空间站 (Sun Nov 10 20:09:18 2013, 美东)
或者只有在一个州有效?因为没有American Society of Clinical Pathology认证.这
样的证书如何找工作?
C A P I T A L C O L L E G E
Address:
1980 Gallows Road
Tysons Corner, VA 22182
i**[email protected]
Tel: 703-356-9898
Fax: 703-356-2460
© 2010-2013
Capital College
Capital College is certified by the State Council of Higher Education for
Virginia
SCHOOL PROGRAM
ACADEMICS
Capital College offers ... 阅读全帖
t***a
发帖数: 45
17
来自主题: JobMarket版 - Histology Research associate wanted
up up

will
s*******7
发帖数: 160
18
来自主题: JobMarket版 - Histology Research associate wanted
You didn't tell the location. Is it a university or company?
Thanks.

will
t***a
发帖数: 45
19
来自主题: JobMarket版 - Histology technician needed ASAP
up up

In

发帖数: 1
20
主要工作是小鼠脑中接种肿瘤细胞及后续的监控、监测。Title可以是技术员,也可以
是博后。
本项目是与药企合作项目,对于申请者之后找工业界的工作会有帮助。
项目启动时间紧迫,所以优先当地的申请者,且只考虑有相关经验的申请者。
工作单位:Brigham and Women's Hospital
工作地点:Longwood medical area。
有意者请直接通过BWH申请,申请链接:
https://partners.taleo.net/careersection/jobdetail.ftl?job=3017
Vy0Utcf5HhI.gmail
详细说明:
Job Description
TECHNICAL RESEARCH ASSISTANT II / 40 HOURS / DAY / BWH - NEUROLOGY - (
3017380)
Description
GENERAL SUMMARY/OVERVIEW STATEMENT:
The TRA II will be involved in the exciting preclinical project to develo... 阅读全帖
d**********0
发帖数: 13081
21
来自主题: Living版 - The 300
这个学校是天才的摇篮, 300 中占了15名。。
The Harker School, San Jose, CA
Dixit, Shikhar, 17
Immunomodulation by Human Retinal Pigment Epithelial Cell Line ARPE-19
Jin, Andrew, 17
Shedding Light on Human Evolution: Machine Learning Algorithms for
Systematic
Genome-wide Discovery and Characterization of Adaptive Mutations
Kuditipudi, Rohith, 18
Network-based Integration of High Throughput Gene Expression and Methylation
Data
Lin, David, 17
Physical Properties and Evolution of
Lin, David, 17
Physical Properties an... 阅读全帖
o******t
发帖数: 1144
22
来自主题: Medicine版 - 收集的丙肝信息
因为老爸得了HCV, 所以才关心这个病. 收集了一些信息, 与大家共享.
美国有1.8%的人携带感染丙肝(HCV). HCV一般会在体内呆10-30年, 但是越早控制越好.
美国疾病控制中心(CDC) 的HCV报告指出:
如果 1. ALT水平不断提高的话; 2. 查出HCV RNA; 3. 肝活检(biopsy) 有炎症的话, 应
该采取治疗.
对于以下的情况是否采取治疗则不清楚 (unclear) – 1. 年龄在18岁以下或60岁以上;
2, 尽管ALT水平不断提高, 但组织学(histologic)变化不严重的确(这种情况每隔3-5年进
行肝活检(biopsy)和ALT的测量, 可以替代干扰素的治疗)
对于以下的情况则不建议治疗 – 1. 正常而持续的ALT值.
美国国家健康研究所 (NIH)的HCV报告指出:
目前的治疗是以alpha-干扰素和Ribavirin结合的治疗, 周期是24周, 或者48周.
Ribavirin自身对HCV不起作用, 但是和干扰素一起使用, 会起到2到3倍的功效. 目前多采
用两者一起使用. 两者一起用48周的有效率是55%, 单使用干扰素的有
t*******n
发帖数: 4445
23
来自主题: Medicine版 - 刚接到家里的电话:
The cure rate is pretty low, although the actual survival time for an
individual is hard to tell (esp. without more detailed information).
Blindly using the latest expensive drug is not a good idea. Your family
should get as much info as possible (histology type, spreading range,
general health), in order to see if surgery, chemo etc. are applicable or
worthwhile (i.e. with clearly defined possible benefit outweighing side
effects) in his case.
Of course there is the other way of conservative
c**i
发帖数: 6973
24
Well, I do not know what to say.
(1) Originally from Taiwan and never having been to China, I have lived in
US for a quarter century. I am a biologist by training, however I have not
kept up with cirrhosis for as long as I can remember--because
(a) I have not had cirrhosis; and
(b) I was a carrier of hepatitis B virus, but after sometime, it dissppeared
(my immune system killed it, as happened to most hepatitis B infection).
(2) The medicine in China is very questinable.
Firstly, the training of... 阅读全帖
l*h
发帖数: 4124
25
来自主题: Medicine版 - 请问关于胃间质瘤
most GISTs are benign on histology but almost all are malignant in
biological behavior. they have a high recurrence rate. on average, one in 5
or 6 recur in a year after complete surgical resection if not treated with
TKIs.
based on the size, this one should have a fairly high recurrence probability
. imatinib can be considered for recurrence reduction.
c**t
发帖数: 465
26
来自主题: Medicine版 - 请问关于胃间质瘤
请问你说的tki中文是什么?药是格格列位吗?请问如果复发还可以再切除吗?癌变和
转移的机率大吗?谢谢

most GISTs are benign on histology but almost all are malignant in
biological behavior. ........
★ Sent from iPhone App: iReader Mitbbs Lite 7.56
l*h
发帖数: 4124
27
craniopharyngioma is one of the tumors with benign histology but malignant
behavior.
if at all possible, perform a total or subtotal resection. she will likely
need hormone replacement therapy. she will possibly need radiotherapy too.
for the surgery, the approach depends on the location and size of the tumor.
the most commonly used two approaches are subfrontal and transphenoid for
this area.
"十年存活率才有70%左右" is about right.
i******4
发帖数: 72
28
I am really sorry for you niece.
My cousin has the same type of tumor (颅咽管瘤 Craniopharyngiomas are
dysontogenic tumors with benign histology and malignant behavior). The
symptoms presented when she was 24 years old. The bad thing about the "
benign" tumor is the high rate of recurrence.
She had the operation by the best surgeon at Tiantan Hosptial in Beijing,
but it came back 4.5 years later. Now her situation is worse than the first
time.
My aunt worries about her a looot. She asked me to sear... 阅读全帖
i******4
发帖数: 72
29
I am really sorry for you niece.
My cousin has the same type of tumor (颅咽管瘤 Craniopharyngiomas are
dysontogenic tumors with benign histology and malignant behavior). The
symptoms presented when she was 24 years old. The bad thing about the "
benign" tumor is the high rate of recurrence.
She had the operation by the best surgeon at Tiantan Hosptial in Beijing,
but it came back 4.5 years later. Now her situation is worse than the first
time.
My aunt worries about her a looot. She asked me to sear... 阅读全帖
l*h
发帖数: 4124
30
来自主题: Medicine版 - 肠癌求助,非常感谢!
their 结肠低分化神经内分泌癌 could be correct, but i am not convinced by the
evidence presented. say the two common markers, what percentage is 部分?
what distribution and percentage is called 灶性? without proper description,
you don't even know whether they saw them on tumor cells or some normal
tissue.
in addition, there is almost no histological grading information. there is a
lot more than 低分化 in grading. what size are the cells? what do the
nuclei look like? what do the cytoplasms look like?
f****o
发帖数: 2770
31
来自主题: Medicine版 - 问个乙肝的药
http://www.gastrojournal.org/article/S0016-5085(06)00735-9/abst
疗效文献原文
可以下载pdf
Histologic improvement occurred in 55% (68/124) of entecavir-treated vs 28%
(32/116) of lamivudine-treated patients (P < .0001). More patients on
entecavir than lamivudine achieved the composite end point: 55% (77/141) vs
4% (6/145), respectively (P < .0001). Mean change from baseline in HBV DNA
was -5.11 log(10) copies/mL for entecavir-treated patients and -0.48 log(10)
copies/mL for lamivudine-treated patients (P < ... 阅读全帖
l*h
发帖数: 4124
32
来自主题: Medicine版 - 肝癌 HCC needs expert opinion
I don't question the diagnosis of HCC, but the pathology report is of very
low quality, and I don't understand the treatment strategy either.
1. there is no description of microscopic findings, there is no way to tell
if the histological grading is correct or wrong. it seems almost all
pathologists in China have the magic power to give an unequivocal diagnosis
on everything.
2. stomach stromal tumor is rare, spleen stromal tumor is extremely rare. i
simply cannot believe all three tumors occur a... 阅读全帖
m****u
发帖数: 275
33
来自主题: Medicine版 - 急问关于淋巴瘤
You likely have a CD30+ T cell lymhoma. The flow cytometry was not read
properly. I would recommend you to have a biopsy with flow cytometry and
histology done asap. Its better to have it done in the USA but insurance may
be a problem since the diagnostic fees alone will be in the thousands. Go
to a better hospital in China.
m*****a
发帖数: 2160
34
来自主题: Medicine版 - 面筋过敏
这个还是由小儿胃肠科的医生决定吧
endoscopy对三岁小孩的风险总体很小,负面影响几乎可以忽略
而且有文献报道单纯试吃不能诊断celiac
"symptom-response to a gluten-free diet alone is not sufficient to diagnose
celiac disease because these features do not distinguish between celiac
disease and "nonceliac gluten sensitivity," which is defined as dose-
dependent intolerance to gluten-containing grains after exclusion of celiac
disease or wheat allergy"
出自:Leffler D, Schuppan D, Pallav K, et al. Kinetics of the histological,
serological and symptomatic respons... 阅读全帖
l**x
发帖数: 110
35
下面这个患者希望能得到版上医生专家的指导意见,危险多大,如何治疗,预后如何?
患者44岁,男,6年前因为肝癌做了肝移植 (符合米兰标准),之后无灾无病好几年。
去年2016年初发现轻微贫血,血色素12,因为当时在服小剂量阿司匹林,于是停服,看
看有没好转。托托拉拉到了年底,还是贫血,血色素10.5。医生说做个内镜。于是在胃
内发现一个肿瘤,内镜医生直觉是GIST。随后CT,但CT却没有异常。4月初第二次胃镜
,GIST依然还在,取样活检表明可能肝癌转移或者是非常罕见的HAC。第二次CT 报告大
小46x35mm,说比较第一次32mm变大了。可能第一次看片医生那天喝多了?肿瘤位置在
Fundus,非常接近GOJ。
AFP好久没有查了,因为肝上的情况一直很好,肝移植前AFP 100多,移植后AFP一直小
于2。上一次至少是2,3年前。也是疏忽了。现在AFP 254. 显然胃上这个要么是转移,
要么是HAC。
下周二医生还要安排一次PETCT,周三见外科医生,估计排除转移外就安排切除了。 求
这里的专家教授指点,这个情况如何是好?下面我贴上最近几个报告。
个人感觉肝移植后5,6年转移到胃实在... 阅读全帖
c**i
发帖数: 6973
36
来自主题: NextGeneration版 - 朋友气疯了~
You are funny.
(1) "第一次我大哥就给我下猛药." I wonder what it really means. Hmm.
(2) I assume you are a woman. So it is kind of odd for you to claim "充血充
了两小时."
(a) Naturally the term* applies not just to men. A woman can be "red-blooded
"--the clitoris of a woman is counterpart of a penis in terms of embryology,
anatomy, histology and physiology.
* 充血 can be translated in English as "engorged with blood" or congestive.
The former is apt in this sexual context--I guess it is what you mean--
whereas the l
l*****e
发帖数: 110
37
re-pap is only to confirm whether you have HPV infection and whether you
have abnormal cells, which you have done in your first pap. If both are
negative, it is good. otherwise you still need further examination.
Colposcopy is not hurt at all. Your OB just check you cervical under
colposcopy to find out any visible changes in your cervical. If your OB see
any abnormal change, she will take a small piece of the tissue (biopsy) for
histology diagnosis, which will tell you the stage of the disease.
a**y
发帖数: 118
38
http://www.prnewswire.com/news-releases/ongoing-investigations-
Well-known Whistleblower Calls for Withdrawal of British Medical Journal
Article Alleging Fraud
AUSTIN, Texas, Nov. 10, 2011 /PRNewswire/ -- The Strategic Autism Initiative
(SAI), an autism research foundation, reported today that an ongoing
investigation into the allegations of fraud against Dr. Andrew Wakefield
concerning a paper published in The Lancet in February, 1998 is being
conducted by an internationally respected scientist... 阅读全帖
a**y
发帖数: 118
39
这是那个指控医生的记者写的反驳文章。所以事情还是无法下定论。
不过我还是相信,即使mmr和autism有关联,不打mmr的风险更大。
http://briandeer.com/solved/david-lewis-1.htm
The Lewis accusations analysed
HEADING “MARKETING DRUGS WITH TABLOID SCIENCE”
DEER: The title is revealingly silly and insulting.
TEXT HEADED “COMMENTARY”
DAVID L LEWIS: “Editors at the British Medical Journal (BMJ) and Brian Deer
, a freelance reporter with no formal training in science or medicine…”
DEER: I’m an experienced investigative reporter with more than 25 years of
contentio... 阅读全帖
w*****s
发帖数: 230
40
现在准备带我家宝宝到外地看病寻求3rd opinion. 终于跟他的主治医生谈崩了。他们
彻底否定我家宝宝有faod,2nd opinion认为他应该是faod,但是需要进一步测试确诊。
几个月前就问了目前就诊的医院有关宝宝的liver tissue,答复是frozen stored in
paraffin block. 我对这些不懂,也就告诉3rd opinion医院有frozen liver tissue.
这家医院就要了,结果被告知没有。
然后我们联系医院,收到不同的答复:
第一次,什么都没有了。。 就算有也不会给外面。
第二次,有一些没有用完,可是不是frozen的。
第三次,有一些没有用完,不是frozen的,根本就没有frozen tissue in paraffin
block这个东西。
目前我们给州卫生机构file了complaint。 但是因为医院很大,top15全美,影响力非
同一般。就算州机构未必会秉公办事,我们需要知道一般这种biopsy tissue怎么处理。
我们目前的信息:
0.1mm-core needle sample两份
应该总长度是2cm x 2 ... 阅读全帖
t**x
发帖数: 20965
41
顶一下

诊。
m******j
发帖数: 5079
42
样品要么做冷冻的block, 要么是福尔马林处理了以后做paraffin block。
也可以一半样品做这个处理,一半样品做那个处理,不知道是他们没说清楚,还是你没
听明白。不会是冷冻的paraffin block.
转MedicalCareer和Medicalpractice,你看看那里有没有人知道。

诊。
s********8
发帖数: 619
43
是的,要么冷冻block, 要么paraffin block(石蜡包埋).石蜡样品是不用冷冻的.
w*****s
发帖数: 230
44
是不是冷冻的还有生物活性,而石蜡处理的就没有了?
如果这样,是不是应该在etiology不清楚的情况下,应该冷冻处理组织呢?
我是一点儿都不懂,打电话就听人家说frozen tissue in paraffin block stored up
to 10 years。 那么人家的意思是不是 frozen or paraffin?
这个有没有活性那么重要, 难道没有规章制度?
另外就是继续问那个读片子的问题,有看法吗?谢谢
m******j
发帖数: 5079
45
具体需要哪种block,应该是医生看需要决定的吧。
石蜡的容易保存,很多的测试都可以做。
冷冻block用于一般细胞表面蛋白的检测。
要哪种block还和抗体的敏感度有关。
此外样品也可以用液氮冷冻保存,这样的组织是有活性的。
我觉得可能的是他们用了一半液氮保存,用了一半做了block。
你可以再确认一下是不是这样。冷冻样品转地方很麻烦,有很多规定。
具体到你这个事例,我不懂,还是得问那一行的专家。取的样品从长度看有4厘米,可
是直径很小,总量感觉不大。

up
m******j
发帖数: 5079
46
http://en.wikipedia.org/wiki/Steatosis
从wiki看,第二张图是石蜡切片,解释了石蜡切片溶解脂肪粒后留下的空。
你们多google,应该可以找到更多信息。

诊。
w*****s
发帖数: 230
47
谢谢,看不懂,总是乱糟糟一大片。我们有切片,但是没有图片。
w******8
发帖数: 141
48
You may also consider:
1. Re-biopsy
2. Genetic test and genetic consultation

诊。
s********8
发帖数: 619
49
有些assay用石蜡切片就可以了,有些要用冷冻切片.不是说石蜡的就没有生物活性,要看
具体assay来确定.你知不知道更多关于你们要用的assay的信息?
m******j
发帖数: 5079
50
从wiki看,石蜡切片染色也可以判断脂肪代谢异常。
他们给你的切片估计是染过的,图片得通过显微镜摄像得到。你的切片如果是粉红色或
浅紫色的,就是染过的,如果是雾白的,那是没染过的。你拿切片请第三方看看。
第三个医院不是说可以通过其他测试来做的吗?问他们需要什么。实在不行,那就象楼
上说的在第三个地方再让他们取个样。
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