p*****m
发帖数: 7030 | 1 恩 我是外行随便问问。我有点不理解的是 搞neuron发育的比较直接 因为neuron总
是有分类的（不同的layer 不同的projection map，不同的neurotransmitter等等
） 这样也许可以用transcription code分类然后看他们分别怎么起源和分化的。但
是astrocytes（或者说glia）好像分类不像neuron那么清楚把 这样除了能study这
些细胞整体是哪来的还有啥别的能搞得没 |
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p*****m
发帖数: 7030 | 2 阿 这个是另外一个问题 搞清楚glia什么功能当然有趣。我是说从发育角度 搞gli
al development还有什么特别有意思的问题没有
such
and |
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p*****m
发帖数: 7030 | 3 就是说起源都不清楚？我只有个很简单的印象就是glia也从neural progenitor ce
ll来 中间有七七八八的fate determination factor参与。呵呵 我基本对发育问题
的细节知道的是零。。而且好像oligodendrocyte比astrocyte的发育搞得清楚些？ |
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f***4
发帖数: 886 | 4 Freda Miller 不算这个领域的吧。glia中包括的很多，发育模式道理上应该是不一样
的。
看来，这个方向确实很多东西未知阿
recent |
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M**a
发帖数: 4816 | 5 Postdoctoral and medical fellow positions are available to participate in
ongoing studies of molecular and cellular neurobiology in developmental and
aged related brain disorders as well as clinical biomarker searching/
validation in Alzheimer’s disease. Candidates with a strong background in
molecular biology, including molecular cloning, vector design and
construction, enzyme assays and purification or cell biology, including
primary cell cultures of neurons, glia, endothelial cells and stem c |
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a****d
发帖数: 1919 | 6 for Ca2+ P method, make sure the pH~ 7.04; after adding the mixed plamids,
buffer, wait only half hour, then wash out the transfection medium as clean
as possible(you should be able to see only the black aggregates on the cell
surface, but not other places), then add fresh warm culture medium.
You can also try Fugene HD from Roche, which is better and less toxic
compared to lipofectamin and similar products.
you can also grow cortical neurons on top of glia cells to preserve activity
and help ce |
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z*t
发帖数: 863 | 7 问个问题
可不可以在fish里做tissue specific cancer,比如brain tumor,可以在神经系统里特
异KO/overexpress tumor supressor/oncogene来建立cancer model，我知道有人
manipulate fly glia,成功的在fly里引入了glioma模型。fish目前有类似mice的cre-
loxP系统么？ |
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s**2
发帖数: 1287 | 8 呵呵，借大牛的名气来做个广告。:)
Third Annual Tufts Neuroscience Symposium & William Shucar
Thursday May 12, 2011 9:00 AM - 5:00 PM
The Department of Neuroscience is hosting the third annual Tufts
Neuroscience Symposium & William Shucart Lecture on Thursday 5/12/11. All
talks will be in the Behrakis Auditorium, located in the Jaharis Family
Center for Biomedical Education and Nutrition Sciences at 150 Harrison
Avenue, Boston MA 02111. For directions and a campus map, please click here.
Lunch will be provided f... 阅读全帖 |
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a********k
发帖数: 2273 | 9 JCR Year and Edition: 2010 Science
Abbreviated Journal Title ISSN {2010} Total Cites Impact Factor
5-Year Impact Factor Immediacy Index {2010} Articles Cited Half-
Life Eigenfactor Score Article Influence Score
CA-CANCER J CLIN 0007-9235 9801 94.262 70.216 8.667 18
3.8 0.04923 24.782
ACTA CRYSTALLOGR A 0108-7673 13944 54.333 24.717 0.629 70
6.2 0.04... 阅读全帖 |
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n********k
发帖数: 2818 | 10 If I am not too out of date, I thought one perfect/classical example is newt
, and even the myofiber can be dedifferentiated and prolfirate again...maybe
reactive glia could be another potential one... Also, in germ lines...many
of these stuff (including EMT etc) are happening naturally, but how
frequently, how easy etc etc is another issue...I think ys ago your boss
together with the other two cows wrote a nice commentary on the natural de-
differentiation and transdifferentiation hype/hope?...... 阅读全帖 |
|
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s*******m
发帖数: 24 | 12 glia没有关系，只要是 GABAergic and Glutaminergic 这两种中只有一种表达就好。 |
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v********m
发帖数: 3531 | 13 whole cell时resistance多大？
为什么要抑制glia，怎么做的？
voltage clamp可以看到Na电流吗？ |
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g*********n
发帖数: 364 | 14 大概200-300M欧母。
抑制glia的是在培养2天后加的，胶质细胞生长剂。
如何在VOLTAGE CLAMP 下记录NA电流？我们一直做电流钳，封上细胞后就转到电流钳了
。不知道电压钳下如何记录NA电流。 |
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u**********d
发帖数: 573 | 15 不想让它们分化，就给它们mitogen，如楼上说的fgf2和egf。但是NSC传不了几代就会
自发分化的，要是这时候还给mitogen，glia就会大量地冒出来。 |
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C********4
发帖数: 308 | 16 假如下面的文章都是同一个人发表的。
请问在这个领域算是上等，还是中等，还是比较差的？
＝＝＝＝＝＝＝＝
1.
Metallic gold slows disease progression, reduces cell death and induces
astrogliosis while simultaneously increasing stem cell responses in an EAE
rat model of multiple sclerosis.
Pedersen DS, Fredericia PM, Pedersen MO, Stoltenberg M, Penkowa M, Danscher
G, Rungby J, Larsen A.
Histochem Cell Biol. 2012 Jul 22. [Epub ahead of print]
2.
Metallic silver fragments cause massive tissue loss in the mouse brain.
Locht LJ, Pedersen MØ, Markhol... 阅读全帖 |
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c********n
发帖数: 67 | 17 Neuron Glia Biol. 2009 May;5(1-2):1.
Those enigmatic NG2 cells . . . .
Richardson WD.
H***[email protected]
Thanks for your kindly help. |
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a**e
发帖数: 5094 | 18 是先分化成neuron和gial cells，再沿着radial glia前行，还是边分化变前行？
以前学的统统都还给老师了 |
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a**e
发帖数: 5094 | 19 是先分化成neuron和gial cells，再沿着radial glia前行，还是边分化变前行？
以前学的统统都还给老师了 |
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h*******r
发帖数: 182 | 20 前辈:
冒昧打扰, 一向可好?
我们有个小组准备今年申请, 现在做case presentation 的练习, 需要高年住院及
Attending指点. 能否请您来给讲解评点一下,大约1 小时, 3-4 cases. 具体时间看您
方便.
多谢了.
This question was one of my interested topics. To my understanding,
progenitors cells migrate along radial glia within VZ. Once they decide to
differentiate, they migrate away into destined areas. Migration is just part
of the differentiation program. However there are situations that migration
and maturation can be uncoupled.
Huskystar |
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n*********b
发帖数: 140 | 21 The maturation of post-migration cortical neurons is likely coupled with the
expansion of glia cells shortly after birth.
there |
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i*****e
发帖数: 633 | 22 就算不在乎debris的话，神经元密度实在是太低太低了。周末去不了实验室，周一放假
。周二我再去看看。希望能再多点吧。
神经元是不是有个soma,有processes的就一定是了？有些glia长的和神经元差不多啊。 |
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w******y
发帖数: 4871 | 23 我好多年没有做neuron培养了,neuron 和glia的大小上是有区别的吧.
neuron有axon and dendrite.
完了完了,第一年修的histology, neuroanatomy都忘掉了.....俺好几年不做动物实验
了,现在开始做人身上的实验了,要是半年后再出不了什么好结果,我就不干research了.
哦,那个"1"肯定是neuron,毕竟俺看了几年的猴脑切片,我以前的几位导师中有一位是做
电极插在活猴脑里的,还要事先训练猴子,猴子可聪明了,还会从我手心里拿东西吃.
你为什么要从化学转到生物上来啊? 化学不是不太难找工作的吗? |
|
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S**********g
发帖数: 109 | 25 就是阻断glia cell的活动，神经系统有两种细胞，一种是神经细胞也就是神经元，另
外一种就是glial cell也就是神经胶质细胞，想找到一种抑制剂，只抑制神经胶质细胞
但不抑制神经细胞 |
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b*********e
发帖数: 870 | 26 还是没看懂。你要inhibit glia 的啥活动？分裂？移动？还是别的啥？ |
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h*******o
发帖数: 4884 | 27 直接换到Neurobasal media +B27 去养，这样基本glia就长不了了 |
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v*******e
发帖数: 11604 | 28 哎，这两天恶补了神经细胞运输问题和睡觉问题，就给大家科普一下吧。
2013年的一篇文章（忘了那个杂志了，好像是nature）说，睡觉的时候老鼠脑子里面细
胞间隙扩大60%，方便脑脊液流动，测得细胞间废物清除速度增加了两倍。所以这个可
能是为什么需要睡觉。
神经细胞的axon确实是需要运输，这个60年代以来就有很多研究了。线粒体都能运，还
是双向的交通。可以运输线粒体，神经递质，细胞膜的蛋白质，细胞内那些线啊纤维啊
的构件都运。运输有快慢两种，就好像火车有快车慢车，慢车开起来也很快，但是要经
常停站。快车就直达，所以比慢车快两个数量级。线粒体和神经递质是快车运的，线啊
纤维啊的构件是慢车运的。
还有人认为axon里面有蛋白质合成，不光是靠细胞核附近合成的。理由包括，不少蛋白
质半衰期短，还没运到就烂掉了。还有些实验证据。又有人认为，神经细胞末端的mRNA
可以来自周围支持的glia细胞。 |
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n*******l
发帖数: 190 | 29 http://www.ncbi.nlm.nih.gov/pubmed/25700174
Science. 2015 Feb 19. pii: aaa1934. [Epub ahead of print]
Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-
seq.
Zeisel A1, Manchado AB1, Codeluppi S1, Lönnerberg P1, La Manno G1, Jur
éus A1, Marques S1, Munguba H1, He L2, Betsholtz C3, Rolny C4, Castelo-
Branco G1, Hjerling-Leffler J5, Linnarsson S5.
Author information
Abstract
The mammalian cerebral cortex supports cognitive functions such as
sensorimotor integration, m... 阅读全帖 |
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l********n
发帖数: 260 | 30 Rank full journal title Total Cites IF
1 ca-a cancer journal for clinicians 18,594 115.84
2 new england journal of medicine 268,652 55.873
4 lancet 185,361 45.217
6 nature biotechnology 45,986 41.514
7 nature 617,363 41.456
12 nature materials 64,622 36.503
13 jama-journal of the american medical association 126,479 35.289
14 nature reviews immunology 28,938 34.985
15 nature nanotechnology 34,387 34.048
16 ... 阅读全帖 |
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w*e
发帖数: 740 | 31 请教大家做utero－virus注射的时候都用什么样的病毒呀？我想尽量特异性地感染增值
的radial glia cells，永久和持续性表达都要做。
谢谢 |
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m*****e
发帖数: 129 | 32 Rank Full Journal Title Total Cites Journal Impact Factor
1 CA-A CANCER JOURNAL FOR CLINICIANS 20,488 131.723
2 NEW ENGLAND JOURNAL OF MEDICINE 283,525 59.558
3 NATURE REVIEWS DRUG DISCOVERY 25,460 47.12
4 LANCET 195,553 44.002
5 NATURE BIOTECHNOLOGY 48,650 43.113
6 NATURE REVIEWS IMMUNOLOGY 31,545 39.416
7 NATURE MATERIALS 72,306 38.891
8 NATURE REVIEWS MOLECULAR CELL BIOLOGY 36,784 38.602
9 NATURE 627,846 ... 阅读全帖 |
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w**m
发帖数: 4061 | 33 ft 这个单词我一直读成 micro-glia 拆着读 不过貌似也都知道 |
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m*********k
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m*********k
发帖数: 10521 | 35 "[STOCK]
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e***o
发帖数: 344 | 38 glial cells 对大多数neuroscientist来说，直接就被忽略不管了。随着技术手段的提
高，比如在glial cells弄chr2等等， glial cells的作用被渐渐的挖掘开来了。。。
想和大家讨论一下，glial cells到底是确实功能上底neuron一等呢？还是不好做，比
如不好patch，杂乱等等。。。。
这里求一片文章，谢谢，好像很有意思的哦。
http://onlinelibrary.wiley.com/doi/10.1002/glia.21096/pdf
Roles of glial cells in neural circuit formation: Insights from research in
insects
l******[email protected] |
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n***w
发帖数: 2405 | 39 This is a very interesting area to explore.
The contributions of glial cells (astrocytes, oligodendrocytes and microglia
) are more than what they originally were thought.
I have a particular interest in neuron-glia interactions and their outcomes
(neuroprotection in my case).
Also, gliovascular relationship should not be neglected neither since
astrocytes can form end-feet structures on endothelial cells which are
important for BBB integrity and nutrient supply. |
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n***w
发帖数: 2405 | 40 From my personal view, I think anti-oxidant capacity and metabolic coupling
are the two important roles. I don't know which one is the most important.
Also, early reactive gliosis forming scar may inhibit inflammation spread.
But persistent gliosis is bad.
I don't know how dopamine hyperactivity is connected to glia. Would you
please refer me an article?
Thanks.
suggested |
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m****g
发帖数: 530 | 42 在自然界中，某些动物天生就具有让人惊叹的再生能力，如蝾螈，如果断了一条腿，不
久以后便能长出一条新腿。斑马鱼
也能够在鳍断开的位置重新长出新的鳍。
相比之下，人类却只有最基本的再生能力。因此，科学家希望能通过对这些动物的研究
，最终找到修复或再生受损组织的
方法。
在一项发表于《Proceedings of the National Academy of Sciences》杂志的研究报
告中，密西根大学的研究人员利用模
式生物斑马鱼发现，斑马鱼的不同组织的再生过程中，某些基因是相同的，如斑马鱼鳍
的再生和心脏修复过程中的基因，
在眼睛的光受体的再生中也发挥作用。这表明，不论身体的哪部分受损，其再生机制可
能由一种共同的分子机制控制。
研究人员将斑马鱼置于强光下短暂处理，破坏斑马鱼眼睛中的光受体。这类似于人眼被
太阳光灼伤，如果损伤较严重，往
往会致人失明。但斑马鱼却能很快长出新的神经元修复损伤。
那么这些新形成的神经元是从什么分化而来呢？研究人员猜测可能来自于视网膜的一种
Müller glia细胞，这种猜测随后在
另一名研究人员的实验中得到证实。
在这项新的研究中，研究人员Zhao |
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