g*********d
发帖数: 233 | 1 科学家呼吁关注全球基因组数据库污染
样品处理有可能是导致DNA数据库广泛污染的最主要原因
2月16日发表在《公共科学图书馆·综合》（PLoS ONE）期刊上的一份研究报告称康涅
狄格大学的遗
传学家Mark Longo及同事发现由顶级公共测序机构提供的测序结果构建的基因组数据库
中的大约
1/5的细菌、植物和非灵长类动物基因组数据受到了人类DNA的污染，样品处理有可能是
导致DNA数据
库广泛污染的最主要原因。这一研究报告引起了生物研究人员及各大权威媒体的高度关
注，《科学
家》（The Scientist）杂志以及《自然》（Nature）杂志均在其官方网络上第一时间
对这一事件
进行了报道。
Mark Longo等在报告中呼吁科学家们需更加努力以确保测序获得的基因组不受到污染，
并应对来自
公共基因组数据库的基因组进行潜在污染检测。
“基因组污染是一个大问题，但却不是一个新问题，”加州大学进化生物学家、美国能
源部联合基因组
研究所系统发育基因组学计划负责人Jonathan Eisen说：“这篇论文或可帮助提醒人们
注意这一问
题。”
污染有可能在测序的任何一个阶段导入到基因组序列中... 阅读全帖 |
|
m***T
发帖数: 11058 | 2 NGS市场又要热闹了。
http://www.bio-itworld.com/news/09/17/12/Sequencing-giants-BGI-
Sequencing Giants BGI and Complete Genomics Announce Merger Agreement
By Bio-IT World Staff
September 17, 2012 | In a stunning move for the genomics industry, two
leading DNA sequencing service providers – China’s BGI and Complete
Genomics – have entered into a definitive merger agreement.
In a prospective deal worth some $117 million, BGI Shenzhen has tendered an
offer of $3.15/share for all of Complete’s outstanding ... 阅读全帖 |
|
s**u
发帖数: 9035 | 3 发信人: shmu (shmu), 信区: Biology
标 题: Opening Faculty position in human genomics, UMB
发信站: BBS 未名空间站 (Mon Oct 17 08:04:37 2011, 美东)
Faculty position in human genomics at the Institute for Genome Sciences,
University of Maryland, Baltimore
The Institute for Genome Sciences is seeking a new faculty member at
the Assistant/Associate/ Professor level in the area of human genomics/
bioinformatics.
The Institute currently has 23 highly collaborative faculty working on
the human microbiome, human genome ... 阅读全帖 |
|
s**u
发帖数: 9035 | 4 Faculty position in human genomics at the Institute for Genome Sciences,
University of Maryland, Baltimore
The Institute for Genome Sciences is seeking a new faculty member at
the Assistant/Associate/ Professor level in the area of human genomics/
bioinformatics.
The Institute currently has 23 highly collaborative faculty working on
the human microbiome, human genome sequencing, genetic variation,
cancer genomics, host/pathogen interactions, and other areas of
interest related to modern genomics... 阅读全帖 |
|
s**u
发帖数: 9035 | 5 Faculty position in human genomics at the Institute for Genome Sciences,
University of Maryland, Baltimore
The Institute for Genome Sciences is seeking a new faculty member at
the Assistant/Associate/ Professor level in the area of human genomics/
bioinformatics.
The Institute currently has 23 highly collaborative faculty working on
the human microbiome, human genome sequencing, genetic variation,
cancer genomics, host/pathogen interactions, and other areas of
interest related to modern genomics... 阅读全帖 |
|
p******9
发帖数: 2 | 6 Job Description
Please send you CV to j******[email protected] if you interested.
Genomics Scientist
This position is based in Shanghai, China.
Duties and Responsibilities:
• Develop and validate PCR and Molecular diagnostic assays (including
both blood and tissue biomarkers) for Genomics projects.
• Participate in scientific activities and initiatives in support of
global Laboratory Operations in the Genomics Department.
• Participate in developing/identifying new strategies, techn... 阅读全帖 |
|
d***n
发帖数: 12 | 7 寻找Genomics方面的专家
我们也想借未鸣空间的宝地，公开求贤，寻找Genomics方面的专家，共同创业。
专业方向:
Next Gen DNA sequencing
Roche 454 Genome Sequencer FLX Standard and FLX Titanium
Solexa technology
Applied Biosystems SOLiD
Whole Genome Analysis
PCR Amplicon Sequencing
Metagenomics
Transcriptomics
Small RNA Analysis
Epigenetics
Genome sequencing and re-sequencing
ABI 3730xl, 3130xl
Amersham MegaBACE 4000 and 1000
文库构建
cDNA libraries (including standard cDNA library, large insert cDNA library,
normalized cDNA library and subtracted c |
|
n********b
发帖数: 27 | 8 A Genomics Center/Lab is seeking 1 qualified highly motivated bioinformatics
candidate in the field of bioinformatics, computational biology and/or
genomics. The position is open immediately upon a qualified candidate is
identified. The position requires a diverse range of expertise in data
mining, machine learning, statistics, biological database manipulation and
biology. The successful candidates must have a MS or a PhD degree or
equivalent experience in bioinformatics, computational biology,
... 阅读全帖 |
|
d***n
发帖数: 12 | 9 寻找Genomics方面的专家
我们也想借未鸣空间的宝地，公开求贤，寻找Genomics方面的专家，共同创业。
专业方向:
Next Gen DNA sequencing
Roche 454 Genome Sequencer FLX Standard and FLX Titanium
Solexa technology
Applied Biosystems SOLiD
Whole Genome Analysis
PCR Amplicon Sequencing
Metagenomics
Transcriptomics
Small RNA Analysis
Epigenetics
Genome sequencing and re-sequencing
ABI 3730xl, 3130xl
Amersham MegaBACE 4000 and 1000
文库构建
cDNA libraries (including standard cDNA library, large insert cDNA library,
normalized cDNA library and subtracted c |
|
d***n
发帖数: 12 | 10 寻找Genomics方面的专家
我们也想借未鸣空间的宝地，公开求贤，寻找Genomics方面的专家，共同创业。
专业方向:
Next Gen DNA sequencing
Roche 454 Genome Sequencer FLX Standard and FLX Titanium
Solexa technology
Applied Biosystems SOLiD
Whole Genome Analysis
PCR Amplicon Sequencing
Metagenomics
Transcriptomics
Small RNA Analysis
Epigenetics
Genome sequencing and re-sequencing
ABI 3730xl, 3130xl
Amersham MegaBACE 4000 and 1000
文库构建
cDNA libraries (including standard cDNA library, large insert cDNA library,
normalized cDNA library and subtracted c |
|
n********b
发帖数: 27 | 11 There is a Research Associate II position opening in a Genomics-Microarray
Core in a Cancer Center in southern CA-Los Angeles Area. Please send your
resume/CV to o****[email protected], if you are interested
in it. Thanks.
See job descriptions:
Job Descriptions
Research Associate II
Functional Genomics Core
1. Perform bench work related to research projects & services using
different genomic technologies including Affymetrix GeneChip, Agilent, Roche
NimbleGen and Illumina’s HiScanSQ microarray platfo... 阅读全帖 |
|
a******a
发帖数: 283 | 12 any trick to reduce the noise generated by the genomic DNA background? There
is no gene of interest in the genome, the primers only anneal to the viral
gene in the plasmid. I added the genomic DNA as background to generate
standard curve for future study (when measure genes instered in the genome).
However there is a lot of background when added the genomic DNA.
Primers work fine with only plasmids as DNA substrate. |
|
n********b
发帖数: 27 | 13 A Genomics Center/Lab is seeking 1 qualified highly motivated bioinformatics
candidate in the field of bioinformatics, computational biology and/or
genomics. The position is open immediately upon a qualified candidate is
identified. The position requires a diverse range of expertise in data
mining, machine learning, statistics, biological database manipulation and
biology. The successful candidates must have a MS or a PhD degree or
equivalent experience in bioinformatics, computational biology,
... 阅读全帖 |
|
n********b
发帖数: 27 | 14 A Genomics Center/Lab is seeking 1 qualified highly motivated bioinformatics
candidate in the field of bioinformatics, computational biology and/or
genomics. The position is open immediately upon a qualified candidate is
identified. The position requires a diverse range of expertise in data
mining, machine learning, statistics, biological database manipulation and
biology. The successful candidates must have a MS or a PhD degree or
equivalent experience in bioinformatics, computational biology,
... 阅读全帖 |
|
n********b
发帖数: 27 | 15 A Genomics Center/Lab is seeking 1 qualified and highly motivated
bioinformatics candidate in the field of bioinformatics, computational
biology and/or
genomics. The position is open immediately. The position requires a diverse
range of expertise in data mining, machine learning, statistics, biological
database manipulation and
biology. The successful candidates must have a MS (encouraged to apply) or a
PhD degree or equivalent experience in bioinformatics, computational
biology, biostatistics a... 阅读全帖 |
|
n********b
发帖数: 27 | 16 A Genomics Center/Lab is seeking 1 qualified highly motivated bioinformatics
candidate in the field of bioinformatics, computational biology and/or
genomics. The position is open immediately. The position requires a diverse
range of expertise in data mining, machine learning, statistics, biological
database manipulation and biology. The successful candidates must have a MS
or a PhD degree or equivalent experience in bioinformatics, computational
biology, biostatistics and genomics and will have ... 阅读全帖 |
|
m*********D
发帖数: 1727 | 17 请教行家：Feng Zhang的genome-wide Screening（CRISPR)后面的deep sequencing
对这个很感兴趣。读了几篇相关的文章和screening,前面的library--infection--drug
screening--genomic DNA extraction都懂。后面的deep sequencing部分就糊涂了。
理论上讲，resistant的细胞一定是有基因改变了的，我一直以为挑单克隆，一个一个
去作整个genome测序。仔细读了文章，是药物筛选几天后，细胞pool一起，再作deep
sequence；对照没有drug筛选（Non-resistant)的组，找出那些被CRISPR改变了的gene
，再确认。
几个问题：
1。这个deep sequencing是测（整个）genomic DNA，还是测留下来的guide sequence
（在lentiviral vector上面）？
2。可以测mRNA/cDNA序列来代替genomic DNA吗？突变的基因总该表现在mRNA上（先不
考虑其他类型的RNA）。
3。因为是pool一起的，不... 阅读全帖 |
|
n********b
发帖数: 27 | 18 A Genomics Center/Lab is seeking 1 qualified highly motivated bioinformatics
candidate in the field of bioinformatics, computational biology and/or
genomics. The position is open immediately upon a qualified candidate is
identified. The position requires a diverse range of expertise in data
mining, machine learning, statistics, biological database manipulation and
biology. The successful candidates must have a MS or a PhD degree or
equivalent experience in bioinformatics, computational biology,
... 阅读全帖 |
|
n********b
发帖数: 27 | 19 A Genomics Center/Lab is seeking 1 qualified highly motivated bioinformatics
candidate in the field of bioinformatics, computational biology and/or
genomics. The position is open immediately upon a qualified candidate is
identified. The position requires a diverse range of expertise in data
mining, machine learning, statistics, biological database manipulation and
biology. The successful candidates must have a MS or a PhD degree or
equivalent experience in bioinformatics, computational biology,
... 阅读全帖 |
|
n********b
发帖数: 27 | 20 A Genomics Center/Lab is seeking 1 qualified and highly motivated
bioinformatics candidate in the field of bioinformatics, computational
biology and/or
genomics. The position is open immediately. The position requires a diverse
range of expertise in data mining, machine learning, statistics, biological
database manipulation and
biology. The successful candidates must have a MS (encouraged to apply) or a
PhD degree or equivalent experience in bioinformatics, computational
biology, biostatistics a... 阅读全帖 |
|
d***n
发帖数: 12 | 21 寻找Genomics方面的专家
我们也想借未鸣空间的宝地，公开求贤，寻找Genomics方面的专家，共同创业。
专业方向:
Next Gen DNA sequencing
Roche 454 Genome Sequencer FLX Standard and FLX Titanium
Solexa technology
Applied Biosystems SOLiD
Whole Genome Analysis
PCR Amplicon Sequencing
Metagenomics
Transcriptomics
Small RNA Analysis
Epigenetics
Genome sequencing and re-sequencing
ABI 3730xl, 3130xl
Amersham MegaBACE 4000 and 1000
文库构建
cDNA libraries (including standard cDNA library, large insert cDNA library,
normalized cDNA library and subtracted c |
|
d***n
发帖数: 12 | 22 寻找Genomics方面的专家
我们也想借未鸣空间的宝地，公开求贤，寻找Genomics方面的专家，共同创业。
专业方向:
Next Gen DNA sequencing
Roche 454 Genome Sequencer FLX Standard and FLX Titanium
Solexa technology
Applied Biosystems SOLiD
Whole Genome Analysis
PCR Amplicon Sequencing
Metagenomics
Transcriptomics
Small RNA Analysis
Epigenetics
Genome sequencing and re-sequencing
ABI 3730xl, 3130xl
Amersham MegaBACE 4000 and 1000
文库构建
cDNA libraries (including standard cDNA library, large insert cDNA library,
normalized cDNA library and subtracted c |
|
d***n
发帖数: 12 | 23 寻找Genomics方面的专家
我们也想借未鸣空间的宝地，公开求贤，寻找Genomics方面的专家，共同创业。
专业方向:
Next Gen DNA sequencing
Roche 454 Genome Sequencer FLX Standard and FLX Titanium
Solexa technology
Applied Biosystems SOLiD
Whole Genome Analysis
PCR Amplicon Sequencing
Metagenomics
Transcriptomics
Small RNA Analysis
Epigenetics
Genome sequencing and re-sequencing
ABI 3730xl, 3130xl
Amersham MegaBACE 4000 and 1000
文库构建
cDNA libraries (including standard cDNA library, large insert cDNA library,
normalized cDNA library and subtracted c |
|
n********b
发帖数: 27 | 24 A Genomics Center/Lab is seeking 1 qualified highly motivated bioinformatics
candidate in the field of bioinformatics, computational biology and/or
genomics. The position is open immediately. The position requires a diverse
range of expertise in data mining, machine learning, statistics, biological
database manipulation and biology. The successful candidates must have a MS
or a PhD degree or equivalent experience in bioinformatics, computational
biology, biostatistics and genomics and will have ... 阅读全帖 |
|
n********b
发帖数: 27 | 25 A Genomics Center/Lab is seeking 1 qualified highly motivated bioinformatics
candidate in the field of bioinformatics, computational biology and/or
genomics. The position is open immediately upon a qualified candidate is
identified. The position requires a diverse range of expertise in data
mining, machine learning, statistics, biological database manipulation and
biology. The successful candidates must have a MS or a PhD degree or
equivalent experience in bioinformatics, computational biology,
... 阅读全帖 |
|
n********b
发帖数: 27 | 26 A Genomics Center/Lab is seeking 1 qualified highly motivated bioinformatics
candidate in the field of bioinformatics, computational biology and/or
genomics. The position is open immediately upon a qualified candidate is
identified. The position requires a diverse range of expertise in data
mining, machine learning, statistics, biological database manipulation and
biology. The successful candidates must have a MS or a PhD degree or
equivalent experience in bioinformatics, computational biology,
... 阅读全帖 |
|
n********b
发帖数: 27 | 27 A Genomics Center/Lab is seeking 1 qualified highly motivated bioinformatics
candidate in the field of bioinformatics, computational biology and/or
genomics. The position is open immediately upon a qualified candidate is
identified. The position requires a diverse range of expertise in data
mining, machine learning, statistics, biological database manipulation and
biology. The successful candidates must have a MS or a PhD degree or
equivalent experience in bioinformatics, computational biology,
... 阅读全帖 |
|
n********b
发帖数: 27 | 28 A Genomics Center/Lab is seeking 1 qualified and highly motivated
bioinformatics candidate in the field of bioinformatics, computational
biology and/or genomics. The position is open immediately. The position
requires a diverse range of expertise in data mining, machine learning,
statistics, biological database manipulation and
biology. The successful candidates must have a MS (encouraged to apply) or a
PhD degree or equivalent experience in bioinformatics, computational
biology, biostatistics a... 阅读全帖 |
|
n********b
发帖数: 27 | 29 A Genomics Center/Lab is seeking 1 qualified highly motivated bioinformatics
candidate in the field of bioinformatics, computational biology and/or
genomics. The position is open immediately. The position requires a diverse
range of expertise in data mining, machine learning, statistics, biological
database manipulation and biology. The successful candidates must have a MS
or a PhD degree or equivalent experience in bioinformatics, computational
biology, biostatistics and genomics and will have ... 阅读全帖 |
|
x********6
发帖数: 986 | 30 【 以下文字转载自 Biology 讨论区 】
发信人: xiao170216 (xiaofeixiang), 信区: Biology
标 题: 请教大家做genome research的病人样本量问题
发信站: BBS 未名空间站 (Wed Apr 17 17:26:11 2013, 美东)
最近计划要开始做一个project是用几个deep sequencing方法来检测一种先天遗传疾病
（已被辨认是由一个控制染色体重构的单基因突变造成的）的基因组层面的缺陷（包括
甲基化，乙酰化，长距离转录等等）， 刚从其他搞临床的实验室获得了三个不同病人
（分别具有该基因的不同突变位点）的细胞样品。
这几天阅读了几篇发表在genome research和Nucleic Acids Research的几篇类似的文
章发现这些工作都测了大概几十个病人的样品。一方面由于我们实验室是一个basic
research lab，无法获得更多的人类临床样本，另外一方面目前的funding也不能支持
做太多样品的deep sequencing。
因此想请教一下各位大侠想genome research， Nuc... 阅读全帖 |
|
k****o
发帖数: 728 | 31 一个Proteomics/Genomics 方面的open access journal，我正在handle一个稿子，需
要寻找几个reviewers。文章title/abstract如下。杂志也不是多好，但也能派上用场
。感兴趣的可站内联系，我需要尽快发出邀请，已经拖了几天了。
请给出name, institute, position, email, expertise, and representative
publications related to this manuscript.
Article Title : Classification of non-animals and invertebrates based on
amino acid composition of complete mitochondrial genomes
Abstract :
Amino acid compositions were predicted from data sets of 47 non-animal and
58 invertebrate animal complete mit... 阅读全帖 |
|
a*******1
发帖数: 1187 | 32 【 以下文字转载自 Pharmaceutical 讨论区 】
发信人: bald (小颓), 信区: Pharmaceutical
标 题: 新泽西公司找有genomics背景的PhD Scientist (转载)
发信站: BBS 未名空间站 (Wed Nov 3 21:40:30 2010, 美东)
发信人: bald (小颓), 信区: Biology
标 题: 新泽西公司找有genomics背景的PhD Scientist
发信站: BBS 未名空间站 (Wed Nov 3 21:06:35 2010, 美东)
公司在找有genomics背景的PhD Scientist.我们是service company,做大量的SNP/
mutation analysis.希望你熟悉 how to look for genomic sequence in the
database, PCR primer design, PCR sequencing data analysis and call mutation/
insertion/deletion etc.如果你有分子生物学的背... 阅读全帖 |
|
|
c*******u
发帖数: 14 | 34 Functional genomics 功能基因组学
鬼子已经几乎完成了水稻基因组的测序工作，
所谓functional genomics是指：
Development and application of global experimental
approaches to assess gene function by making use of
the information and reagents provided by structural
genomics(see Functional Genomics in Plants, 1998,
Plant Physiol. 118:725-732) |
|
Z**********g
发帖数: 222 | 35 感觉很promising,可以解决不少争议问题:
1.CSC or clonal evolution:我觉得cancer genomics数据还是比较支持clonal
evolution模型的.从现有的ancer genomics数据来看,任何一个肿瘤里面可能存在成千
上万的突变,而且各个突变(包括点突变,拷贝数,重排等)的发生率迥异,表明随机突变和
克隆进化必然存在.这种genetic heterogeneity不太容易由CSC模型解释.毕竟CSC模型
从本质上来说更强调epigenetics.另外,把一个肿瘤里的CSC和nonCSC拿出来测序对比,
我觉得突变图谱肯定也不一样(虽然目前我还没有看到这方面的证据)
肿瘤本质上是基因病,根源是基因组不稳定性,所以我支持clonal evolution.
2.The relationship between primary and metastasis:对于转移,一个关键的问题是
早期还是晚期事件.如果转移发生在早期,原发瘤和转移瘤应该是平行进化,那么各自的
突变图谱应该比较divergent,而发生在晚期的话,应该convergence... 阅读全帖 |
|
y***i
发帖数: 11639 | 36 今天刚知道我需要找的是人的centromere序列或者位置（那哥们昨天说错了）。
1. 知道怎么找么？
2. 或者有什么地方可以找到？昨天有人给我一段mysql找老鼠的centromere
mysql --user=genome --host=genome-mysql.cse.ucsc.edu -A -N -e "show tables;"
mm9 \
| grep _gap | \
while read T; do mysql --user=genome --host=genome-mysql.cse.ucsc.edu -A -N
-e "select * from $T" mm9; done \
| sort -k8 | cut -f2- | head -24
我把mmp替换成hg19但没有结果出来，看来格式不同。你知不知道怎么改一下？
多谢。学到了些东西。 |
|
S*****P
发帖数: 323 | 37 http://news.sciencemag.org/scienceinsider/2011/06/sequence-yiel
DNA Sequence Yields Clues to Germany's 'Super Toxic' E. coli outbreak
by Martin Enserink on 2 June 2011, 5:50 PM |
Just from the high number of deaths and severe cases, scientists and public
health experts battling Germany's massive E. coli outbreak knew they were up
against something unusual. Now, early results from sequencing projects of
the enterohemorrhagic Escherichia coli (EHEC) strain appear to confirm that
a never-before-see... 阅读全帖 |
|
k****o
发帖数: 728 | 38 一个Proteomics/Genomics 方面的open access journal，我正在handle一个稿子，需
要寻找几个reviewers。文章title/abstract如下。杂志也不是多好，但也能派上用场
。感兴趣的可站内联系，我需要尽快发出邀请，已经拖了几天了。
请给出name, institute, position, email, expertise, and representative
publications related to this manuscript.
Article Title : Classification of non-animals and invertebrates based on
amino acid composition of complete mitochondrial genomes
Abstract :
Amino acid compositions were predicted from data sets of 47 non-animal and
58 invertebrate animal complete mit... 阅读全帖 |
|
W*****o
发帖数: 1780 | 39 做了一个基因的3'RACE，用的clontech试剂盒，发现扩出来的片段和genome DNA一样。
巧合的是genome DNA里有一段区域A含量很高，是不是可能oligodT正好match上，RT的
时候把污染的genomic DNA给扩出来了。（Reverse Transcriptose可能扩出DNA吗？）
又或者oligodT留在反应体系里，在后面的PCR的时候作为一条引物把genome DNA给扩出
来了。
那种可能性更高呢？ |
|
a******a
发帖数: 283 | 40 We want to detect a gene of interest inserted into human genome by
retrovirus, using qPCR. That said human genome will be in the background and
the gene of interest should have no overlap with human genome. However the
gene of interest is high GC content (around 70%). Currently we are building
a standard curve by mocking the reaction condition, but the result is not
that well.
Any recommendation? Anybody familiar with detecting gene insert in the
genome? Any recommendation regarding good literat... 阅读全帖 |
|
n********b
发帖数: 27 | 41 There is a genomics wet-lab full-time position opened in the Center for
Genomics at Loma Linda University, CA. This position, supported by hard
money, has a great potential of future career development with excellent
benefits including full medical insurance. The candidates with a Bachelor (
with many years of molecular biology/NGS experience) or a MS or a PhD in
life science,and Optional Practical Training (OPT) are encouraged to apply.
If you are interested, please send your resume/CV to Dr.... 阅读全帖 |
|
c*********r
发帖数: 1312 | 42 求内行科普一下，做3D genome，测序样本制备需要多少细胞或者细胞核？需要哪些好
的抗体？还需要哪些关键试剂或者方法？
我问这些问题是因为之前和一哥们儿聊过一些。他做AP后其中一个方向研究细胞周期中
的3D genome变化。需要找一个实验模型，要至少几个million的细胞，而且是高度同步
的。虽然使用物理和化学方法可以是培养的细胞同步，但是我总觉得会不会有一些
artifact。
我倒是有个很好的系统，某种动物胚胎发育过程中，前3个细胞周期天然高度同步，而
且从一个个体就可以拿到几个million个胚胎，关于cyclin的诺奖甚至都是在该物种里
最早发现的，可见很适合研究细胞周期。这种动物的进化地位其实比果蝇线虫更接近人
。但是问题在于这个系统不是主流模式生物，genome虽然在06年就发表了，后来也有不
断改进，但是很不完善。而且很多抗体估计都没有人在这个物种里开发和尝试过。考虑
到种种风险，我的同学决定还是上保险靠谱的动物，先做老鼠和人的细胞吧，就没有跟
进了。
各位评价一下这样的系统是否可以用来研究3D genome在细胞周期中的变化？ |
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发帖数: 1 | 43 xenopus吧 百万细胞可以啊
上Hi-C不要抗体啊
推荐你做targeted 这样测序只要几百个million reads
像liebermann那样一下测几个billion 测序量惊人 计算也很困难
[在 cellcreator (cellcreator) 的大作中提到：]
:求内行科普一下，做3D genome，测序样本制备需要多少细胞或者细胞核？需要哪些好
:的抗体？还需要哪些关键试剂或者方法？
:我问这些问题是因为之前和一哥们儿聊过一些。他做AP后其中一个方向研究细胞周期
中的3D genome变化。需要找一个实验模型，要至少几个million的细胞，而且是高度同
步的。虽然使用物理和化学方法可以是培养的细胞同步，但是我总觉得会不会有一些
:artifact。
:我倒是有个很好的系统，某种动物胚胎发育过程中，前3个细胞周期天然高度同步，而
:且从一个个体就可以拿到几个million个胚胎，关于cyclin的诺奖甚至都是在该物种里
:最早发现的，可见很适合研究细胞周期。这种动物的进化地位其实比果蝇线虫更接近
人。但是问题在于这个系统不是主流模式生物，genome虽然在06年... 阅读全帖 |
|
j****0
发帖数: 55 | 44 The Genome Science Group (B-6) in the Bioscience Division at Los Alamos
National Laboratory sponsors internships for post baccalaureate and graduate
students year-round. The program lasts two to six months. Students will
leave the program with the hands-on training from B-6 scientists that will
equip them with advanced technical knowledge and skills in one of the
following areas:
• Genomic Finishing
• Annotation and Analysis
• Comparative Genome
• Metagenome
& |
|
j****0
发帖数: 55 | 45 The Genome Science Group (B-6) in the Bioscience Division at Los Alamos
National Laboratory is seeking highly motivated postdoctoral researchers to
work on our Bioinformatics team to perform specialized analyses on newly
sequenced microbial genomes and Metagenomes. The work will involve running
sequence analysis software, analyzing genes by DNA/protein sequence, and
genomic structure, mining various gene annotation databases and preparing
manuscripts. The successful candidate will work closely |
|
g******1
发帖数: 295 | 46 工作地点在湾区，有兴趣的话发简历给hzheng At guardanthealth.com
ABOUT THE ROLE
In this role, you’ll wear multiple hats and get involved in a variety of
projects. Your main focus will be to help grow Guardant Health’s patient
referral business, helping connect physicians and their patients with
clinical trials based on the genomic alterations found using Guardant360.
You will be part of the Medical Affairs team but work closely with BioPharma
Business Development to facilitate this service.
Other projects will in... 阅读全帖 |
|
a****y
发帖数: 1035 | 47 【 以下文字转载自 Biology 讨论区 】
【 原文由 aubrey 所发表 】
Published online May 1, 2003: http://www.sciencemag.org/feature/data/sars/
Characterization of a Novel Coronavirus Associated with Severe Acute
Respiratory Syndrome
In March 2003, a novel coronavirus (SARS-CoV) was discovered in association
with cases of severe acute respiratory syndrome (SARS). The sequence of the
complete genome of SARS-CoV was determined, and the initial characterization
of the viral genome is presented in this report. The genome of |
|
p*****p
发帖数: 61 | 48 【 以下文字转载自 Biology 讨论区 】
发信人: pharlap (梦), 信区: Biology
标 题: 求审稿机会(生物)－－genomics, gene expression regulation, molecular biology
发信站: BBS 未名空间站 (Sat May 19 14:40:34 2012, 美东)
生物博士后，准备申请绿卡，需要积累审稿数量。
尝试给杂志编辑写了几封信没有回音，双管其下，也到这里来寻求帮助
领域关键词：
genomics,medical genomics,gene expression regulation, molecular biology,NGS
data analysis
希望得到推荐
有意帮忙者站内信件联系
非常感谢 |
|
a****y
发帖数: 1035 | 49 Published online May 1, 2003: http://www.sciencemag.org/feature/data/sars/
Characterization of a Novel Coronavirus Associated with Severe Acute
Respiratory Syndrome
In March 2003, a novel coronavirus (SARS-CoV) was discovered in association
with cases of severe acute respiratory syndrome (SARS). The sequence of the
complete genome of SARS-CoV was determined, and the initial characterization
of the viral genome is presented in this report. The genome of SARS-CoV is
29,727 nucleotides in length, h |
|
s***n
发帖数: 9 | 50 NEW YORK, Aug. 6 (Genome Technology) - Are you paid what you're worth? Would
you be better off in a bigger company? At a government agency? With a more
advanced degree?
After culling through the data from GT's first-ever genomics salary survey, we
tell you who gets the best pay by job title, company size, and sector (public
or private). Results come from a survey conducted this summer of nearly 12,000
readers of Genome Technology magazine - a group comprised of scientists who
work for pharmaceut |
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